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Lab One: A beginner’s guide to the spikerbox Brain Cells need to communicate with each other to control the body A brain with only one neuron is not a brain A brain is a network (friendship) of neurons How do neurons talk? Method 1: Chemical communication e.g. Bacteria Con: slow, limited by diffusion How do neurons talk? Method 2: Chemical communication through synapse e.g. stretching the neuron to cover more space Con: still slow, signal must travel long way through cell How do neurons talk? Method 3: Electrical and chemical combination Electricity carries signal quickly down long axon to synapse, chemical transmission at synapse to the next neuron Electrical Neural Communication: The Action Potential Action potentials (“spikes”) are generated by Na infusion depolarizing the cell membrane and K expulsion repolarizing it. Spike “moves” as depolarization propagates down axon Anatomy of the Neuron Wikipedia.org Model of the nervous system: Discoid cocroach (Blaberus discoidalis) Neuroanatomy of Cockroach Neuroanatomy of Cockroach Leg Procedures: Put the cockroach in a jar of ice water Procedures: Cut off one of roach’s legs near the body (Put petroleum jelly on exposed wound on body and leg, return cockroach to house, leg will grow back if cockroach not full grown adult) Procedures: Place leg on cork of SpiderBox, with a bit of leg overhang Procedures: Put one electrode in the femur and the other in the coxa Procedures: Observe the spike trains, demonstrating neuron communication Classify spikes by amplitude: binning Entering Your Data Condition No Stimulation Toothpick Stimulation Blowing Through Straw Method Notes Timing Notes