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“HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON CEREBRUM, CEREBELLUM, PONS AND MEDULLA OBLONGATA IN GOAT (Capra hircus)” THESIS Submitted in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY IN VETERINARY ANATOMY AND HISTOLOGY BY AMOL MADHUKAR SALANKAR Enrollment No: V/02/1828 NAGPUR VETERINARY COLLEGE, NAGPUR MAHARASHTRA ANIMAL AND FISHERY SCIENCES UNIVERSITY, NAGPUR- 440006 (INDIA) 2017 DECLARATION OF STUDENT I hereby declare that the experimental research work and interpretation of the thesis entitled “HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON CEREBRUM, CEREBELLUM, PONS AND MEDULLA OBLONGATA IN GOAT (Capra hircus)” or part thereof has not been submitted for any other degree or diploma of any University nor the data have been derived from any thesis / publication of any University or scientific organization. The sources of materials used and assistance received during the course of investigation have been duly acknowledged. Date: Signature (AMOL MADHUKAR SALANKAR) Enroll. No. V/02/1828 Dr. R. S. DALVI Chairman, Advisory Committee DECLARATION OF ADVISORY COMMITTEE AMOL MADHUKAR SALANKAR has satisfactorily prosecuted his course of research for a period of not less than two semester and that the thesis entitled “HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON CEREBRUM, CEREBELLUM, PONS AND MEDULLA OBLONGATA IN GOAT (Capra hircus)” submitted by him is the result of student’s bonafide research work is sufficient to warrant its presentation to the examination in the subject of VETERINARY ANATOMY AND HISTOLOGY for the award of DOCTOR OF PHILOSOPHY degree by Maharashtra Animal and Fishery Sciences University, Nagpur. We also certify that the thesis or part thereof has not been previously submitted by him for a degree or diploma of any other University. Date: Place: Dr. R.S. DALVI Chairman / Advisor, Professor and Head Veterinary Anatomy and Histology ADVISORY COMMITTEE Name Designation Signature Chairman ……………….. 2) Dr. S. B. Banubakode Member ……………….. 3 Dr. N. C. Nandeshwar Member ……………….. 4) Dr. M. G. Thorat Member ………………... 5) Dr. S. K. Sahatpure Member 1) Dr. R. S. Dalvi ………………... CERTIFICATE This is to certify that the thesis entitled “HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON CEREBRUM, CEREBELLUM, PONS AND MEDULLA OBLONGATA IN GOAT (Capra hircus)” submitted by AMOL MADHUKAR SALANKAR to the Maharashtra Animal and Fishery Sciences University in partial fulfillment of the requirement for the degree of DOCTOR OF PHILOSOPHY has been approved by the student’s Advisory Committee after examination in collaboration with the External Examiner. Name & Signature of External Examiner Dr. R. S. Dalvi Dr. R. S. Dalvi Professor & Head Advisor / Guide Dept. of Anatomy and Histology N.V.C., Nagpur. Professor & Head Dept. of Anatomy and Histology N.V.C., Nagpur. ADVISORY COMMITTEE Name Designation Signature 1) Dr. R. S. Dalvi Chairman ……………….. 2) Dr. S. B. Banubakode Member ……………….. 3) Dr. N. C. Nandeshwar Member …................... 4) Dr. M. G. Thorat Member ………………... 5) Dr. S. K. Sahatpure Member ………………... (Dr. N. P. DAKSHINKAR) Associate Dean Nagpur Veterinary College, Nagpur. ACKNOWLEDGEMENT Undertaking this Ph.D. has been a truly life-changing experience for me and it would not have been possible to do without the support and guidance that I received from many people. I would like to first say a very big ‘thank you’ to my research guide cum chairman, advisory committee Dr. R. S. Dalvi, Professor and Head, Department of Veterinary Anatomy & Histology, Nagpur Veterinary College, Nagpur for his invaluable guidance, constant encouragement and keen interest which immensely helped me throughout the study period. Many thanks also to Dr. S. B. Banubakode, Associate Professor, Department of Veterinary Anatomy & Histology, Nagpur Veterinary College, Nagpur. Without his guidance and constant feedback this Ph.D. would not have been achievable. I greatly appreciate the support received through the members of my advisory committee Dr. N. C. Nandeshwar, Associate Professor, Department of Veterinary Anatomy & Histology, Nagpur Veterinary College, Nagpur, Dr. M. G. Thorat, Associate Professor, Department of Veterinary Surgery & Radiology, PGIVS, Akola and Dr. S. K. Sahatpure, Associate Professor, Department of Animal Reproduction, Obstetrics & Gynecology, Nagpur Veterinary College, Nagpur for their valuable support, suggestions, help and advice from time to time. I am very grateful to Dr. N. P. Dakshinkar, Associate Dean, Nagpur Veterinary College, Nagpur for his support and provisions of all facilities in the college to undertake and complete the research work. I also extend my sincere thanks to Dr. Rupali Y. Charjan and Dr. Umesh P. Mainde, Assistant Professors, Department of Veterinary Anatomy & Histology, Nagpur Veterinary College, Nagpur for their constant guidance, keen interest and valuable suggestions throughout the work. I express my sincere thanks to Mr. S. N. Gawande. University Librarian, MAFSU, Nagpur. I also offer my sincere thanks to Mr. Dinesh Patil, Assistant Professors of Statistics, Department of Veterinary Genetics, Nagpur Veterinary College, Nagpur for their suggestions and guidance as and when required. I was fortunate to get devoted and selfless help from my departmental colleagues Dr. Sirsikar, Dr. Gedam, Dr. Sukhdeve, Sriniwas, Khandate, Pawan Kawareti and Jigyasa Rana at various stages of my research work and my post graduate studies too. I express my gratitude to all of them. There are no words at my commands to pay regards my father Mr. Madhukar and mother Mrs. Vibha, who took pains to bring me up this stage, without their love, inspiration and blessing this Ph. D. could not have been accomplished. I shall be failing in my duties if do not express my gratitude towards my younger brother Dr. Prashant and his wife Dr. Shweta and nephew Sanvi, my father in law Mr. Bhimraoji Chaudhari and mother in law Mrs. Kamal Chaudhari, brother in law Dr. Praful, his wife Dr. Ritu and Dr. Amol Chaudhari for their love, constant support and well wishes, I want to preserve a special love for my relatives and well wishers, which enable me to complete the entire research program successfully. I would always remember the invaluable help, active cooperation and constant inspiration of my wife Dr. Sanjivani and my lovely son Arjun who makes my life happy and memorable and for rendering help during the course of study and making it possible for me to complete what I started. During my study in this esteemed institute I was fortunate to receive the kind of cooperation from almost everyone in one way or other. It is extremely difficult for me to thank all of them individually by names. This short coming may please be pardoned. (AMOL MADHUKAR SALANKAR) TABLE OF CONTENTS CHAPTERS NO. PARTICULARS PAGE NO. 1 INTRODUCTION 1-3 2 REVIEW OF LITERATURE 4-30 3 MATERIALS AND METHODS 31-35 4 RESULTS AND DISCUSSION 36-73 5 SUMMARY AND CONCLUSIONS 74-81 A BIBLIOGRAPHY I-XI B APPENDICES C VITA D THESIS ABSTRACT I-XXIV A a-b LIST OF TABLES Sr. No. Table Page No. 1 Biometrical observation showing of brain in group I and group II. 1 2 Biometrical showing different indices of brain in group I and group II. 2 3 4 5 6 7 8 9 10 11 12 Micrometrical observation showing thickness of different layers of frontal lobe of cerebral cortex in group I and group II (µm). Micrometrical observation showing thickness of different layers of temporal lobe of cerebral cortex in group I and group II (µm). Micrometrical observation showing thickness of different layers of occipital lobe of cerebral cortex in group I and group II (µm). Micrometrical observation showing neuronal count of different layers of frontal lobe of cerebral cortex in group I and group II. Micrometrical observation showing neuronal count of different layers of temporal lobe of cerebral cortex in group I and group II. Micrometrical observation showing neuronal count of different layers of occipital lobe of cerebral cortex in group I and group II. Micrometrical observation showing neuron density of different layers of frontal lobe of cerebral cortex in group I and group II. Micrometrical observation showing neuron density of different layers of temporal lobe of cerebral cortex in group I and group II. Micrometrical observation showing neuron density of different layers of occipital lobe of cerebral cortex in group I and group II. Total neuron count of different lobes of cerebral cortex in group I and group II. 3 4 5 6 7 8 9 10 11 12 13 Layer thickness of cerebellar cortex in group I and group II (µm). 13 14 Neuronal count of different layers of cerebellar cortex in group I and group II 14 15 Neuronal density of different layers of cerebellar cortex in group I and group II 15 16 Neuron count, total neuron count and neuronal density in Pons in group I and group II. 16 17 Neuron count, total neuron count and neuronal density in medulla oblongata in group I and group II. 17 LIST OF FIGURE No. Particulars Stain Mag 1 Photomicrograph of showing cerebral cortex with piamater (P) and blood vessel (B) (Gr.I). HE 100 X 2 Photomicrograph of showing cerebral cortex with six layers (Gr.- I, Frontal lobe). Gallocyanine 50 X 3 Photomicrograph of showing cerebral cortex with six layers (Gr.- II, Frontal lobe). Gallocyanine 50 X 4 Photomicrograph of showing cerebral cortex with six layers (Gr.- I, Temporal lobe). Gallocyanine 50 X 5 Photomicrograph of showing cerebral cortex with six layers (Gr.- II, Temporal lobe). Gallocyanine 50 X 6 Photomicrograph of showing cerebral cortex with six layers in group I (Occipital lobe). HE 50 X 7 Photomicrograph of showing different layers of cerebral cortex (Gr.- II, Occipital lobe). Gallocyanine 100 X 9 Photomicrograph of showing small neuron (M) and area calculated by Q-imaging software (Gr.II cerebral cortex). Toluidine blue 1000 X Photomicrograph of showing Large neuron (L) and area calculated by Q-imaging software (Gr.-II cerebral cortex). Toluidine blue 1000 X 11 Photomicrograph of showing spindle shape neuron (N) (Gr.- I cerebral cortex). Toluidine blue 1000 X 12 Photomicrograph of showing pyramidal shape neuron (N) (Gr.- II cerebral cortex). Toluidine blue 1000 X 13 Photomicrograph of showing stellate shape neuron (N) (Gr. -II cerebral cortex). Toluidine blue 1000 X 14 Photomicrograph of showing molecular layer of cerebral cortex (Gr.- I, Occipital lobe). Gallocyanine 100X 15 Photomicrograph of showing molecular layer of cerebral cortex (Gr.- II, Frontal lobe). Toluidine blue 200X 16 Photomicrograph of showing cells of Cajal (C) (Gr. - I, cerebral cortex). HE 200 X 10 17 Photomicrograph of showing external granular layer of cerebral cortex (Gr.- I, Frontal lobe). Toluidine blue 200X 18 Photomicrograph of showing external pyramidal layer of cerebral cortex (Gr.- II, temporal lobe). Toluidine blue 200X 19 Photomicrograph of showing internal granular layer(arrow) of cerebral cortex (Gr.- I, Frontal lobe). Gallocyanin 200X 20 Photomicrograph of showing internal pyramidal layer of cerebral cortex (Gr.- II, temporal lobe) Toluidine blue 200 X 21 Photomicrograph of showing internal pyramidal layer and Betz cells (arrow) of cerebral cortex (Gr.- I, frontal lobe). Toluidine blue 200 X Photomicrograph of showing multiform layer of cerebral cortex and white matter (arrow) (Gr.- II, temporal lobe). Toluidine blue 200 X Photomicrograph of showing molecular layer (M), Purkinje cell layer (P) and Granule cell layer (G) (Gr.-I, cerebellar cortex). HE 200 X Photomicrograph of showing molecular layer (M), Purkinje cell layer (P) and Granule cell layer (G) (Gr.-I, cerebellar cortex). HE 100 X Photomicrograph showing molecular layer (M), Purkinje cell layer (P) and Granule cell layer (G) (Gr.-II, cerebellar cortex). Silver Impregnation 100X 26 Photomicrograph showing arborisation (arrow) from Purkinje cell (Gr.-II, cerebellar cortex). Luxol fast 200 X 27 Photomicrograph of showing Basket (arrow) (Gr.-II, cerebellar cortex). Toluidine blue 400 X 28 Photomicrograph of showing Purkinje cells (arrow) (Gr.-I, cerebellar cortex). HE 200 X 29 Photomicrograph of showing Golgi cells (arrow) (Gr.- I, cerebellar cortex). HE 200 X 30 Photomicrograph of showing Epineurium (E), Perinurium (P) and Oligodendrocytes (arrow) (Gr.-II, Pons). Toluidine blue 200 X Toluidine blue 100 X 22 23 24 25 31 cells Photomicrograph of showing large (L), medium (M) and small (S) neurons (Gr.-I, Pons). 32 Photomicrograph of showing nerve fibres and oligodendrocyte (arrow) (Gr.-II, medulla oblongata). Toluidine blue 200 X 33 Photomicrograph showing large cells (Gr.- II, medulla oblongata). HE 200X 34 Photomicrograph of cerebrum (frontal lobe Gr. I) showing small neuron (N), blood vessel (B) and neuropil (P). PAS reaction 200X Photomicrograph of cerebrum (temporal lobe Gr. I) showing small neuron (N), blood vessel (B) and neuropil (P). PAS reaction 200X Photomicrograph of cerebrum (occipital lobe Gr. I) showing small neuron (N), blood vessel (B) and neuropil (P). PAS reaction 200X Photomicrograph of cerebrum (frontal lobe Gr. II) showing small neuron (N), blood vessel (B) and neuropil (P). PAS reaction 200X Photomicrograph of cerebrum (temporal lobe Gr. II) showing small neuron (N), blood vessel (B) and neuropil (P). PAS reaction 200X Photomicrograph of cerebrum (occipital lobe Gr. II) showing small neuron (N), blood vessel (B) and neuropil (P). PAS reaction 40 Photomicrograph of cerebrum (Gr. I) showing PAS activity in large neuron (L). PAS reaction 400X 41 Photomicrograph of cerebrum (Gr. II) showing PAS activity in large neuron (L). PAS reaction 200X 42 Photomicrograph of cerebellum (Gr.I) showing PAS activity in molecular layer(M), Purkinje cell layer (P),Granular layer and Neuropil (PL). PAS reaction 100X Photomicrograph of cerebellum (Gr. II) showing PAS activity in molecular layer(M), Purkinje cell layer (P), Granular layer and Neuropil (PL). PAS reaction 200X 44 Photomicrograph of pons (Gr. I) showing PAS activity in large neuron (L). PAS reaction 100X 45 Photomicrograph of pons (Gr. II) showing PAS activity in large neuron (L). PAS reaction 200X 46 Photomicrograph of medulla oblongata (Gr. I) showing PAS activity. PAS reaction 100X 35 36 37 38 39 43 200X 47 Photomicrograph of medulla oblongata (Gr. II) showing PAS activity. PAS reaction 100X Photomicrograph of cerebrum (Gr.I) showing acid phosphatase activity in blood vessel (B), neuron (N) and neuropil (P) Acid phosphatase 200X Photomicrograph of cerebrum from (Gr. II) showing acid phosphatase activity in blood vessel (B), neuron (N) and neuropil (P). Acid phosphatase 200X Photomicrograph of cerebellum (Gr. I) showing acid phosphatase activity in molecular layer (M), Purkinje cell layer (P), Granular layer and Neuropil (PL). Acid phosphatase 200X Photomicrograph of cerebellum (Gr. II) showing acid phosphatase in molecular layer (M), Purkinje cell layer (P), Granular layer (G) and Neuropil (PL). Acid phosphatase 100X Photomicrograph of pons from (Gr. I) showing acid phosphatase activity. Acid phosphatase 100X 53 Photomicrograph of pons (Gr. II) showing acid phosphatase activity. Acid phosphatase 100X 54 Photomicrograph of medulla oblongata from (Gr. I) showing acid phosphatase activity. Acid phosphatase 100X 55 : Photomicrograph of medulla oblongata (Gr. II) showing acid phosphatase activity. Acid phosphatase 100X Alkaline phosphatase 200X Alkaline phosphatase 200X Alkaline phosphatase 200X Alkaline phosphatase 200X 48 49 50 51 52 56 57 Photomicrograph of cerebrum (Gr.I) showing alkaline phosphatase activity. Photomicrograph of cerebrum (Gr. II) showing alkaline phosphatase activity. 58 Photomicrograph of cerebellum (Gr. I) showing alkaline phosphatase activity. 59 Photomicrograph of cerebrum (Gr. II) showing alkaline phosphatase activity. 60 Photomicrograph of pons (Gr.I) showing alkaline phosphatase activity. Alkaline phosphatase 100X 61 Photomicrograph of pons (Gr. II) showing alkaline phosphatase activity. Alkaline phosphatase 100X Photomicrograph of medulla oblongata from group I showing alkaline phosphatase activity. Alkaline phosphatase 200X 62 63 Photomicrograph of medulla oblongata (Gr. II) showing alkaline phosphatase activity. Alkaline phosphatase 200X 64 Photomicrograph of cerebrum (Gr. I) showing lipofuscin deposits. Sudan black B 200X 65 Photomicrograph of cerebrum (Gr. II) showing lipofuscin deposits. Sudan black B 200X 66 Photomicrograph of cerebrum (Gr. II) showing lipofuscin deposits and displaced nucleus. Sudan black B 1000X 67 Photomicrograph of cerebellum (Gr. I) showing lipofuscin deposits. Sudan black B 100X 68 Photomicrograph of cerebellum (Gr. II) showing lipofuscin deposits. Sudan black B 1000X 69 Photomicrograph of lipofuscin deposits. showing Sudan black B 400X 70 Photomicrograph of pons (Gr. II) showing lipofuscin deposits. Sudan black B 1000X 71 Photomicrograph of medulla oblongata (Gr. I) showing lipofuscin deposits. Sudan black B 200X 72 : Photomicrograph of medulla oblongata (Gr. II) showing lipofuscin deposits. Sudan black B 200X 73 Transmission electron photomicrograph showing normal neuron from cerebrum (Gr. I) with fatty deposit (arrow) Uranyl acetate and lead citrate X 9.6 M Transmission electron photomicrograph showing normal neuron (cerebrum Gr. I) with electron dense material (arrow). Uranyl acetate and lead citrate X 5.7 M Transmission electron photomicrograph (cerebrum Gr.II) showing granulation (G), pigmentation (P) and myelin degeneration (M) Uranyl acetate and lead citrate X 13.5M Transmission electron photomicrograph showing nerve fiber (M) (cerebrum Gr. II) bulging (B) and dense cytoplasm (C). Uranyl acetate and lead citrate X 12 M Transmission electron photomicrograph showing nerve cell (cerebrum, Gr. II) and displaced nucleus (arrow) Uranyl acetate and lead citrate X 7.7 M Transmission electron photomicrograph showing nerve cell (cerebellum, Gr. I) and fat deposites (arrow) Uranyl acetate and lead citrate X 4.8 M 74 75 76 77 78 pons (Gr. I) 79 80 81 82 83 84 85 86 87 Transmission electron photomicrograph showing nerve cell (cerebellum, Gr. I) and fat deposits (arrow) Uranyl acetate and lead citrate X 4.8 M Transmission electron photomicrograph showing myeline sheath (cerebellum, Gr. I) and deposits (arrow) Uranyl acetate and lead citrate X 57 M Transmission electron photomicrograph showing degenerating neuron (cerebellum, Gr. II) and shrunken mitochondria (arrow) Uranyl acetate and lead citrate X 9.6 M Transmission electron photomicrograph showing degenerating neuron (cerebellum, Gr. II) and shrunken mitochondria (arrow) with dense rim Uranyl acetate and lead citrate X 7.7 M Transmission electron photomicrograph showing apoptic neuron, shrunken mitochondria (M) and regressed axon (arrow), (cerebellum, Gr. II) Uranyl acetate and lead citrate X 6.7 M Transmission electron photomicrograph showing normal neuron (Pons, Gr. I) and very less deposits (arrow). Uranyl acetate and lead citrate X 7.7 M Transmission electron photomicrograph showing degenerative neuron (Pons, Gr. II) and cloudy mitochondria (arrow). Uranyl acetate and lead citrate X 7.7 M Transmission electron photomicrograph showing vesicular appearance of myelin (M) (Pons, Gr. II) and scanty cytoplasm (C). Uranyl acetate and lead citrate X 77 M Transmission electron photomicrograph showing normal neuron (medulla oblongata, Gr. I). Uranyl acetate and lead citrate X 7.7 M LIST OF GRAPHS Sr. No. I II III IV V VI VII VIII IX X XI XII XIII Graph Showing different indices of brain Showing thickness of different layers of frontal lobe Showing thickness of different layers of temporal lobe Showing thickness of different layers of occipital lobe Showing neuronal density of different layers of frontal lobe Showing neuronal density of different layers of temporal lobe Showing neuronal density of different layers of occipital lobe Showing total neuron count in different lobes. Showing neuronal density of different layers of cerebellar cortex Showing thickness of different layers of cerebellar cortex Showing neuronal count in different layers of cerebellar cortex Showing neuronal density in pons. Showing neuronal density in medulla oblongata. 1. INTRODUCTION The goat (Capra hircus) is an important livestock species in India. They are the most adaptable and geographically widespread livestock species. Goat is one of the earliest domesticated animals by human‟s around 10,000 years ago at the dawn of the Neolithic period in the Fertile Crescent (Porter, 1996 and Pringle 1998). Goats played a central role in the Neolithic agricultural revolution and the spread of human civilization around the globe (Legge, 1996 and Zeder and Hesse, 2000). Goats are gaining acceptance as an established model for biomedical research and for surgical training and teaching. They are used in medical, orthopaedic, psychological, chemotherapeutic and physiologic research (Lincicome and Hall, 1984). Goats are easy to transport and they appear to be handier than other members of the ruminant family. Compared with cattle, their small size permits goats to be maintained in a relatively small area. Goats are extensively studied for structural information being considered as a suitable model in small ruminant category. The visceral systems of animals of veterinary importance are extensively studied however the nervous system is scantily studied by the scientist in contrast to human subject. The complex well secured expression of brain is one of the restrictions to easy availability of samples. The present study is undertaken to explore the gross as well as microscopic details of chief components of this vital system. The system is peculiar in the sense, it is lodged in limited well secured place but governs total activity of the body. The system is highly evolved with clear-cut defined functions, which makes imperative to study the structural details of each component of brain. Even the slight change may lead to significant morbid changes in body function. The present study would be helpful to understand the finer details of major brain components with reference to morphology, microstructure, histochemistry and ultrastructure. The cerebrum is the largest part of the brain, associated with higher brain function such as thought and action. The cerebrum is divided into four lobes; these are frontal lobe, parietal lobe, occipital lobe, and temporal lobe. Frontal lobe is associated with reasoning, planning, parts of speech, movement, emotions, and problem solving. Parietal lobe is associated with movement, 1 orientation, recognition, perception of stimuli. Occipital lobe is associated with visual processing, however the temporal lobe is associated with perception and recognition of auditory stimuli, memory, and speech. The cerebral cortex is highly corrugated structure. This makes the brain more efficient, because it can increase the surface area of the brain and the amount of neurons within it. The importance of the cerebral cortex in various motor and cognitive functions has drawn scientist‟s attention to the study of its age-related modifications in the last few decades. Decreases in the functional capacity of the central nervous system with age occur universally in all living organisms. For instance, significant alteration in the gait control, sleeping cycle, and learning and memory with age are the three commonest neural impairments in aged humans. The cerebellum has alike surface configuration to that of cerebrum with finer structure. The cerebellum receives information from the sensory systems, the spinal cord, and other parts of the brain and then regulates motor movements. The cerebellum coordinates voluntary movements such as posture, balance, coordination, and speech, resulting in smooth and balanced muscular activity. The brain stem is responsible for regulation of basic vital life functions such as breathing, heartbeat, and blood pressure. The brain stem is made of the midbrain, pons, and medulla oblongata. The pons is involved in the transmission of signals to and from other structures in the brain, such as the cerebrum or the cerebellum. The pons is also involved in sensations such as hearing, taste, and balance. Medulla oblongata is the caudal most part of the brain stem, between the pons and spinal cord. It is responsible for maintaining vital body functions, such as breathing and heart rate. The medulla oblongata helps to regulate breathing, heart and blood vessel function, digestion, sneezing, and swallowing. Age is an important factor for most common neurodegenerative diseases, including Mild cognitive impairment, Alzheimer's disease, cerebrovascular disease, Parkinson's disease and Lou Gehrig's disease. While much research has focused on diseases of aging, there are few informative studies available on the molecular biology of the aging brain in the absence of neurodegenerative disease. However, research does suggest that the aging process is associated 2 with several structural, chemical, and functional changes in the brain. Considering the importance of brain in the neuroscience, the present study is undertaken with reference to structural details. The objectives of present study are 1. To study the histological changes in the cerebrum, cerebellum, pons and medulla oblongata in young and adult goat. 2. To study the histochemical changes in the cerebrum, cerebellum, pons and medulla oblongata in young and adult goat. 3 2. REVIEW OF LITERATURE 2.1. Brain morphology Olopade et al. (2005) carried out morphometric study on the brains of twenty West African Dwarf (WAD) sheep. The mean brain weight was 69.14g, while the mean brain length and depth were 7.48cm and 4.17cm respectively. The mean length of the cerebrum and cerebellum were 5.08 cm and 2.27cm, respectively. There was a significant difference in the weight of head, weight of brain, brain length and depth and in the relative brain weight. Animals aged one year and above, had significantly heavier body weights and longer cerebrum (p<0.05), than those below this age mark, the latter however had significantly lower relative brain weight. They concluded that the study will be useful in comparative Neuroanatomy and as baseline research data in neuropathology, pharmacology, anaesthesiology and neurophysiology. Olopade et al. (2007) studied neurometrics of the brain of Sahel goat. They found that the mean brain weight was 85.13g. The mean brain length, depth, cerebral length, depth and cerebellar length and depth were 9.38cm, 4.34cm, 5.78cm, 4.34cm, 2.79cm and 2.43cm respectively, while relative brain weight was 0.004. Animals below one year of age and 20kg body weight had significantly higher relative brain weight than animals at and above this age and body weight groups. Female goats had a lower mean brain weight than males. They concluded that there was a strong positive correlation between body weight and brain depth while a strong negative correlation existed between body weight and relative brain weight. Byanet et al. (2008) studied morphometry of brain of 10 African grasscutter. They found that the mean brain weight was 10.5±0.31 gm and mean brain length was 5.95±0.12 cm. The mean brain height was 1.59±0.10 cm. The mean cerebellar weight and length were found to be 1.26±0.05 and 3.48±0.45 cm respectively. They also reported that the ratio of the brain to body weight was 0.01, which was bigger than Red Sokoto sheep, but smaller than of man. They concluded that the increase in the body weight did not directly affect the brain weight. Byanet et al. (2014) recorded the morphometric parameters of brain of male and female grasscutters. They found that the mean brain weights were 4 9.80±0.50 g and 10.27±0.45 g for males and females respectively. The cerebral and cerebellar mean lengths of 3.14±0.04 cm and 1.34±0.04 cm for males, 6.26 ±0.10 cm and 3.80±0.32 cm for females were observed. The mean brain lengths were 5.63±0.07 cm and 6.26±0.1 cm for males and females respectively. They found significant differences in the body and olfactory bulb weights and also, in the whole brain and cerebral lengths between the males and females. Kigir et al. (2010) studied the nerometrics of the sahel goats using a total of 14 goats. They recorded brain weight, weight of the head, length of cerebrum, depth of cerebrum, length of cerebellum and depth of cerebellum were 96.14 kg, 1.19 kg, 7.18 cm, 3.81 cm, 3.42 cm and 2.77 cm respectively. They concluded that the animals more than 2 to 3 years age have slightly higher brain values than those less than 1½ years. The females had lower brain weight than males. Further they stated that the location had no effect on the neurometrical data of the Sahel goats. Olopade et al. (2011) studied the craniofacial indices and neuromorphometrics of the Nigerian local pig. They reported that the mean brain weight, mean brain length, cerebrum and cerebellum lengths, brain and cerebellar heights were 84±12 g, 6.9±1.5 cm, 4.9±1.7 cm, 2.2±1.0 cm, 5.2±0.88 cm and 3.0±1.1 cm respectively. There was a negative correlation between the weights of the animal and the height of the cerebellum, the length of cerebrum and length of the cerebellum and between the weight of the head and height of the cerebellum. They observed a positive correlation between the length of brain and the weight of brain, and between the length of the cerebrum and weight of brain. The cerebral length was statistically longer in the males than the females. They concluded that the data obtained from this study will provide added information in the field of comparative anatomy and porcine neuroanatomy research. Byanet and Dzenda (2014) studied 12 cerebella (6 males and 6 females) to determine the effect of sex and also its relationship to other body variables in adult African giant pouched rats using a quantitative morphometric method. They reported that mean weight of cerebellum was slightly higher in females (0.82 ± 0.03 g) than males (0.76 ± 0.02 g). In females, the cerebellum weight was positively correlated with the brain and the head weights. In males, the cerebellum weight was positively correlated with the body, the head and brain 5 weights. They concluded that the cerebellum in females may be estimated accurately from the brain mass, while in males, it may be used to estimate the body mass. 2.2. Neuronal density and neuron number Shefer (1972) studied absolute number of neurons and thickness of the cortex of areas 6, 10, 18, 21, 21/38 and 40 healthy persons of different ages and in patients with senile and vascular dementia or with Pick's or Alzheimer's disease. He reported that in old age the mean absolute number of neurons in mentally healthy persons was reduced by 20%, while the thickness of the cortex remain unchanged. In persons with senile and vascular dementia, the number of nerve cells was reduced by 35-38 %, but there was no decrease in the thickness of the cortex on its free surface. In Alzheimer's disease the number of nerve cells were reduced by half and the thickness of the cortex by 6% in Pick's disease and was characterized by mass death of nerve cells in the affected areas, leading to a reduction of 14-30 in their number, and there was decrease in thickness of the cortex by half. Rockel et al. (1980) counted number of neuronal cell bodies in a narrow strip (30 micrometers) through the depth of the neocortex in mouse, rat, cat, monkey and man. They reported that in mammalian evolution the area of the neocortex increases in larger brains but the number of neurons through the depth remains constant. They suggested that the intrinsic structure of the neocortex was basically more uniform. Terry et al. (1987) studied changes occurring in neocortical cell populations of normal aging brains. They reported that the total number of neurons, neuronal density and percentage of cell area remain unchanged. They concluded that the aging affects the frontal and temporal lobes more than the parietal. They stated that constant neuronal density coupled with diminished cortical volume (decreased brain weight and cortical thinning) was indicative of some neuronal loss with age. Vincent et al. (1989) studied light and electron microscopic examination of cerebral cortex in well-fixed young (5-6 years) and old (25-35 years) rhesus 6 monkeys to determine the effects of age on neurons. Light microscopic measurements of the mean cortical depth in vertically oriented 1-micron-thick sections revealed no obvious thinning with age, and the mean diameter of neuronal nuclei did not change with age. On the basis of counts of neuronal profiles containing nuclei in 250-microns-wide strips of 1-micron-thick sections passing through the entire depth of the cortex, no significant neuronal loss could be detected. Tigges et al. (1990) studied right or left area 4 in 19 rhesus monkeys, ranging in age from 1 day to 35 years, and assessed age-related changes in the neuronal population. Approximately one-third loss was observed in the total number of neurons in maturing monkeys. They reported no age-associated loss of neurons. Beaulieu (1993) used dissector method to estimate the numerical density of neurons and their actual number per column in the occipital, the parietal and the frontal cortex of adult rat. The numerical density of neurons in the frontal cortex (34,000/mm3) was significantly lower than in the two other neocortical areas (occipital: 52,000; parietal: 48,000/mm3). He also found an alternate distribution of low and high density of neurons from layers II-III to VI in the three cortical areas, with the highest density in layer IV of the two sensory areas. There were more neurons under 1 mm2 of surface in the parietal (90,000) than the occipital or the frontal cortex (71,000). He compared these values from the rat with those previously obtained in cat and monkey, and found that the number of neurons per cortical column was the highest in the sensory area preferentially used by each species. Sholl (1993) made a quantitative investigation of samples under standard conditions from different well-defined anatomical regions of the cerebral cortex in several mammals. He computed the total numbers of neurons in a cylinder of cortex with cross-sectional area 400 µ2. He reported that total number of neuron were about 80-60 in man and cat. The number of perikarya in a similar cylinder of mouse cortex was about 14. Heinsen et al. (1994) counted total nerve cell numbers in the right and left human entorhinal areas by cell density determinations with the optical dissector. They stated that the laminar composition of gallocyanin (Nissl)-stained sections 7 could easily be compared. They observed that the human entorhinal area was quantitatively characterized by an age-related nerve cell loss in pre as well as pri layers. Witelson et al. (1995) studied cytoarchitectonic area in the cortex of the planum temporale in men and women. Cortical depth, the number of neurons through the depth of cortex and the number of neurons per unit volume were obtained for the total cortex and for each of the six layers in each hemisphere. For total cortex in both hemispheres, depth and number of neurons were similar, but neuronal density was greater by 11% in women, with no overlap of scores between the sexes. The sex difference in neuron number was attributable to layers II and IV; in contrast, neuronal density did not differ between the sexes in layers Ill, V, and VI. They suggested that the cortical functional unit had a different ratio of input and output components in men and women which had implications for the sex differences in cognition and behavior. Gazzaley et al. (1997) examined cortex of 17 macaque monkeys, consisting of 3 juvenile (1–2 years old), 8 young adult (8–12 years old) and 6 aged (25–32 years old) monkeys. The results of the quantitative study demonstrated that there were no significant differences in the total number of cortex layer II neurons among juvenile, young adult, and aged monkeys and there was no correlation between neuron number and the age of the animals. They concluded that a significant source of variability in the adult and aged groups was likely to be biological in origin. Peinado et al. (1997) evaluated the quantitative and cytomorphometric effects of aging on neuronal and glial populations in cortex of the rat. They recorded cortical volume, neuronal density, glial density, neuronal area and shapes of the soma and nucleus in cortical layers I, II–IV, V, and VI. They found no changes with age in volume of the cortex or neuronal density. They concluded that the stability of neuronal density together with the increased number of glial cells and the changes in neuronal soma size was suggestive of aged-related cognitive impairment which had a consequence of neuronal dysfunction rather than actual neuronal losses. Greferath et al. (2000) investigated age-related changes in the number and size of neurons in female Dark Agouti rats. There was a 13% reduction in the number of neurons at 17 months compared to six months, and a 30% reduction 8 at 26 months. They recorded 8091±125 neurons at six months of age, 8187±223 at 17 months, 8203±353 at 20 months and 7066±853 at 26 months. They noted no significant differences between any of the four age-groups (six, 17, 20 and 26 months) with regard to the number of neurons. They observed strong correlation between the presence of spatial learning impairment and a reduction in the number of neurons. Peters (2002) stated that human and non-human primates showed cognitive decline during normal aging. This decline was attributed to a loss of cortical neurons, but recent studies have shown that there was no significant cortical neuronal loss with age. Neurons were found to acquire pigment, but the only other obvious changes were in layer 1 of neocortex. Layer 1 became thinner as apical tufts of pyramidal cells lose branches, as well as synapses, and at the same time the glial limiting membrane was found thickened. That might be contributing to cognitive decline because it would cause a slowing of conduction along nerve fibers and disrupting the timing in neuronal circuits. Davanlou and Smith (2004) estimated the total numbers of neurons, glial cells, and endothelial cells in rat cerebral cortex by using unbiased stereological counting techniques and systematic sampling. They devised a method for reducing problems associated with the uncertainties those arise when distinguishing between various types of cells. In a sample of brains, the mean total number of cells (neurons, glial and endothelial) in the syncortex of the rat brain was 128 x 106. These numbers of cells were categorized as 47% neurons, 24% glial cells, 17% endothelial cells, and 11% uncertain cell types. Cullen et al. (2006) suggested that neuronal density in left dorsolateral prefrontal cortex was increased in schizophrenia. They estimated neuronal density, size and shape in the prefrontal cortex of the left and right hemispheres of brains. They found that the mean total neuronal density for the schizophrenia series was 35,000 (s.d.= 6500) per mm3 on the left and 38,200 (s.d.=3600) per mm3 on the right. The corresponding values in control group were 41,500 (s.d.=8600) per mm3 and 36,200 (s.d.=5700) per mm3 respectively. Total neuronal density in control group brain was generally greater in the left hemisphere, while the reverse pattern was observed in the schizophrenia brains. This loss or reversal of asymmetry was most significant in cortical layer 3. Pyramidal neurons in this cell layer were significantly larger on the left and more 9 spherical in shape than on the right side in control brains, but size and shape did not differ between the two sides in schizophrenia. Jelsing et al. (2006a) estimated neocortical cell numbers from the developing pig brain. The postnatal development of neocortical neurons and glial cells from the experimental Gottingen minipig was compared with the postnatal development of neocortical neurons in the domestic pig. A significant postnatal development was observed in the Gottingen minipig brain for both neuronal (28%; P=0.01) and glial cells (87%; P<0.01). A corresponding postnatal development of neurons was not detected in the domestic pig brain. The mean total number of neocortical neurons was 324 million in the adult Gottingen minipig compared with 432 million in the domestic pig. They concluded that the domestic pig seemed to be a more suitable model for evaluating the effects of developmental insults on human brain growth and neuronal development than the Gottingen minipig. Yates et al. (2008) studied neuron number in the medial prefrontal cortex and primary visual cortex of young adult (85–90 days of age) and aged (19–22 months old) male and female rats in order to investigate any age-related losses. Possible sex differences in aging were also examined since sexually dimorphic patterns of aging have been seen in other measures. An age-related loss of neurons (18–20%), which was mirrored in volume losses, was found to occur in the primary visual cortex in both sexes in all layers except IV. They reported that there was loss of neurons (15 %) from layer V/VI of the ventral medial prefrontal cortex and observed decrease in volume of this region in male but not in female. In contrast, dorsal medial prefrontal cortex showed no age-related changes. They stated that the effects of aging clearly differ among regions of the rat brain and to some degree, between the sexes. Diao et al. (2009) examined the density of Nissl-stained neurons in the primary visual cortex of four young adult cats. The results of their study showed that there was no significant difference in the density of Nissl-stained neurons between young and old cats (P>0.05). They concluded that the effect of excitatory transmitter system in the old visual cortex was increased relative to the inhibitory transmitter system, which might cause an imbalance between cortical excitation and inhibition and might be an important factor mediating the visual function decline during aging. 10 Meyer et al. (2010) reported the number and distribution of NeuN-positive neurons within the C2, D2, and D3 TC projection columns in P27 rat somatosensory barrel cortex. They noted a single column with 19,109 ± 444 neurons (17 560 ± 399 when normalized to a standard-size projection column). Neuron density differences along the vertical column axis delineated „„cytoarchitectonic‟‟ layers. The resulting neuron numbers per layer in the average column were 63 ± 10 (L1), 2039 ± 524 (L2), 3735 ± 905 (L3), 4447 ± 439 (L4), 1737 ± 251 (L5A), 2235 ± 99 (L5B), 3786 ± 168 (L6A), and 1066 ± 170 (L6B). These data were then used to derive the layer-specific action potential (AP) output of a projection column. They confirmed that the ensembles of spiny L4 and thick-tufted pyramidal neurons emit the major fraction of APs of a column. The number of APs evoked in a column by a sensory stimulus (principal whisker deflection) was estimated as 4441 within 100 ms post-stimulus. Fischer et al. (2012) quantified the neuronal density in human cerebral cortex categories into young and old age group of equal size of either below or above 60 years of age. They found that the neuronal density in parietal, temporal, occipital, frontal and entorhinal cortex showed a tendency of age-related decline in each area. There was, however, no significant correlation between decreasing neuronal number and increasing age. Age-related changes were most pronounced in the temporal cortex and least obvious for the entorhinal cortex, these regional differences, however, were statistically not significant. Walloe et al. (2014) estimated total number of neurons in 94 normal Danish individuals between 18 and 93 years of age using stereological methods. They found that the total number of neocortical neurons in females was 19 × 109, where as in males it was 23 × 109, which accounts for a sex related difference of 16%. However, the total number of neocortical neurons varies among individuals by more than a factor of two, with a range of 118% (14.7–32.0 × 109 neurons), there was considerable overlap between men and women. They reported reductions in neocortical volume, surface area, white matter, archicortex volume, and brain weight with advancement of age. There were no changes in gray matter volume or neocortical thickness. The change in total number of neocortical neurons from age 18 to 93 years was 9.5%, resulting in an average “loss” of about 85,000 neurons per day. This age-dependent neuronal decrease was equivalent for both sexes. They found that the total neocortical neuron number in 11 individuals between 94 and 105 years of age (seven females, one male) was the same in very old females compared with younger women, group. 2.3. Cerebellar cortex Fox and Barnard (1957) studied cerebellar cortex in the adult monkey (Maccaca mulatta). They recorded quantitative observations and measurements in Golgi preparations and from cell counts and measurements in cresyl-violet preparations. In fixed preparations, there were an average of 510 Purkinje cells per mm2 of Purkinje cell layer and 2.4 million granule cells per mm 3 of granular layer. The ratio of the cross-sectional area of the molecular layer to the granular layer was 1.5 to 1. The average thickness of the granular layer was 0.2 mm. Nandy (1981) studied cerebellar cortices in 4, 10 and 20 year of Macaca nemestrina for the number of Purkinje (P) and granule cells and the deposition of lipofuscin in P cells in relation to aging. Lipofuscin distribution significantly increased within the P cells in these animals. The number of P cells was significantly reduced, while there were no changes in the number of granule cells. It appears from this and other studies that the Purkinje cells were more prone to aging changes than the granule cells of the cerebellum both in lipofuscin formation and cell loss. Although the precise functional significance of these changes in P cells was not clear, their vulnerability may be related to changes in motor function in old age Mwamengele et al. (1993) counted Purkinje neurons in mammalian cerebella. Nucleoli were chosen as the counting unit and numbers were estimated from uniform random samples of wax-embedded tissue sections in the cerebella of rat, rabbit, cat, dog, goat, sheep, pig, ox, horse and human. There was a significant linear relationship between log number and log weight. In Goat Cerebellar Purkinje cells number were 2.691 x 106. Mean cerebellar weight varied from 0.22 g (rat) to 91 g (human) and corresponding numbers of PC nucleoli from 0.25 to 15.7 millions. They analysed brains of females and males separately (cat, goat, pig, ox, horse, human) and found that there were no significant differences between the regression lines. They concluded that, for any given cerebellar weight, females and males had similar numbers of neurons. 12 Renovell et al. (1996) measured the number and volume of granule neurons (GC) in the cerebellar cortex in the human cerebellum for agedependent changes. The total number of GC decline significantly during the aging process. The human cerebellum aging involved a decrease of the number of GC in the granular layer. The group I showed a GC densities of 388±103 x10 3 mm3. While in the group II, this cellular type reduces to 23% (299 ±130 x103 mm3) and in the group III the decrease was greater to 38% (241 ± 66 x103 mm3) respect to the group I. Also the volume of GC was smaller in the group III. Furthermore they demonstrated that there exists a great variability in the GC densities in the II group 43% as compared to a 26% in the group I and 27% in the group III. Sjobeck et al. (1999) studied cerebellum and inferior olive of individuals of very high age. The study group included 15 non demented and basically healthy cases aged 32-104 years. Linear neuronal density was expressed as number of PC per millimeter tissue measured in the vermis and as neuronal numbers per square millimeter tissue in the inferior olive. The linear PC density clearly decreased with increasing age. In a comparison between the centenarian group and non centenarians, the mean PC density in the vermis of the former group was 6.09/mm and of the latter 2.85/mm. The difference between the two groups was significant. They concluded that aging results in reduced PC density in the vermis cerebelli, further accentuated in the very late stages of life. Pal et al. (2003) studied the gross anatomy and histology of the cerebellum of man and fowl. The shape, size, weight, volume, lobar and folial arrangement were considered under gross observation. The cerebellum of fowl presented a well-developed lobe, which represented the vermiform lobe of human cerebellum. They also studied histomorphology of the organ with the help of slides stained with H&E and Golgi-cox method. Large flask shaped Purkinje cells in single row were observed in both the species. The sizes of the Purkinje cells were greater but the population per unit volume was lesser in case of human cerebellum. Purkinje cell numbers in 1mm length in man was found to be 6.6, while Purkinje cell numbers in fowl was 18.9. However both the species presented a common type of histological organization. Jelsing et al. (2006b) estimated total number and perikaryon volume of cerebellar Purkinje cells, in the cerebellar cortex of the Gottingen minipig during 13 postnatal development. The total number of Purkinje cells in neonate to adult ranged from 1.83x106 to 2.82x106. The volume of the cerebellum increased almost four-fold from a mean of 2.45 cm3 (0.048) in neonates to 9.34 cm3 (0.094) in adults. The study demonstrated that a pronounced postnatal neurogenesis in Purkinje cell number and perikaryon volume was part of the growth and development of the cerebellum in the Gottingen minipig. The Purkinje cells of the Gottingen minipig were found to be substantially large compared with human and represents the largest cells described hitherto from mammalian cerebella. Zhang et al. (2006) studied age related changes in the structures of the cerebellar cortex of young adult and old cats. They measured thickness of the cerebellar cortex and the density of neurons in all the layers. There was a significant decrease in the thickness of molecular layer and total cerebellar cortex and significantly increased in the granular layer in old cats. They noted that the Purkinje cells (PCs) showed much fewer NF-IR dendrites in old cats than those in young adults. They further reported that there was loss of neurons and decrease in the number of dendrites of the PCs in the aged cerebellar cortex and correlated it to the functional decline of afferent efficacy and information integration in the senescent cerebellum. Agashiwala et al. (2008) developed a new method for stereologic sampling of the cerebellar cortex in an effort to quantify Purkinje cells in association with certain neurodegenerative disorders. Using this approach, they counted Purkinje cells in the right cerebella of four human male control specimens, aged 41, 67, 70 and 84 years, and estimated the total Purkinje cell number for the four entire cerebella to be 27.03, 19.74, 20.44 and 22.03 million cells, respectively. By this method they compared the density of the cells within the tissue as 266,274, 173,166, 167,603 and 183,575 cells/cm3 respectively. They stated that data demonstrated the accuracy of their approach which offers an improvement over previous methodologies. This approach could be applied to morphometric studies of other tissues. Axelrad et al. (2008) quantitatively assessed the number of Purkinje cells in brains of essential tremor ET patients and similarly aged controls. Calbindin immunohistochemistry was performed on paraffin sections of the cerebellum. Images were digitally recorded and blinded measurements of the number of Purkinje cells per millimeter of cell layer (linear density) were made. Purkinje cell 14 linear density was inversely correlated with age (r=-0.53, P=.006) and number of torpedoes (r=-0.42, P=.04). They demonstrated a reduction in Purkinje cell number in the brains of patients with ET who do not have Lewy bodies. These data further support the view that the cerebellum was anatomically, as well as functionally, abnormal in these ET cases. Whitney et al. (2008) studied autistic and normal brain of 13-54 years of age in human, irrespective of sex. They reported that Purkinje cell counts in normal human were 4.7±0.8 PCs/mm and in autistic brains it was 3.5±1.8 PCs/mm. The decrease in PC density was evident in some cases, the role of PCs in the clinical manifestations of autism remains obscure, since there was no apparent correlation between the density of PCs and the clinical severity of autism. Woodruff-Pak et al. (2010) used unbiased stereology to estimate the total number of Purkinje neurons in cerebellar cortex of mice. Study indicated significant loss of Purkinje neurons in the 18 and 24 month groups. There was a significant effect of age. They reported that the processes of aging impact brain structures and associated behaviors differentially, with cerebellum. They further noted that Post hoc comparisons of the significant age effect using the Turkey HSD test indicated significantly fewer Purkinje neurons in the age groups of 18 and 24 months in comparison with the age groups of 4, 8, and 12 months. Yesmin et al. (2011) calculated age wise change in the number of Purkinje cells in Bangladeshi people. They reported Purkinje cell as 160.71 ± 24.47 in group A (Age 20-29 years) and 152.20 ± 6.49 in group D (age> 50 years), where the mean reduction was 2.5% per decade. Histological studies revealed that the number of Purkinje cell per square mm decreased with age which was statistically significant. Maseko et al. (2012) studied the cerebellar cortex of the African elephant by using a combination of basic neuroanatomical and immunohistochemical stains with Golgi and stereologic analysis. Stereologic analysis revealed that the volume of the somata of the Purkinje cells averaged between 8,186 and 8,903 µm3 across the regions. The Nissl-stained material determined that the density of Purkinje cells varied between 5,894 and 8,812 cells/mm3. The posterior hemispheric and vermal blocks had higher densities than the anterior 15 hemispheric and vermal blocks. The stereologic analysis confirmed that neuronal density was low in the elephant cerebellar cortex, providing for a larger volume fraction of the neuropil. They concluded that quantitatively larger and more complex cerebellar cortex likely represents part of the neural machinery required to control the complex motor patterns involved in movement of the trunk and the production of infrasonic vocalizations. Viswasom et al. (2013) studied the number of Granule cells in the human cerebellar cortex and its quantitative variation with respect to age was studied in seventy human cerebellums using light microscopy. The study revealed a progressive decrease in number of granule cells with increase in the fibre components. They concluded that the number of granule cells showed statistically significant negative correlation with age and the study provides more information regarding the quantitative histological structure of human cerebellar cortex. Louis et al. (2014) quantified cerebellar molecular layer and cellular density in 15 essential tremor (ET) cases, 15 controls, and 7 spinocerebellar ataxia (SCA) cases. They stated that the Purkinje cell count differed across the three groups (p < 0.001), with the highest counts in controls, intermediate counts in ET cases and lowest counts in SCA cases. ET cases and controls had similar molecular layer cellular density (p = 0.79) but SCA cases had higher values than both groups (p < 0.01). A robust inverse correlation between Purkinje cell count and molecular layer cellular density (i.e., brains with more Purkinje cell loss had higher molecular layer cellular density), observed in SCA and controls (r =-0.55, p = 0.008), was not observed in ET cases. Although Purkinje cell counts were reduced in ET cases compared to controls, an increase in molecular layer cellular density was not evident in ET. The increase in molecular layer cellular density, observed in SCA cases, may require a more marked loss of PCs than occurs in ET. 2.4. Size and shape of cell body Games and Winer (1988) studied brain of adult male albino rats. They found that the layer II-III boundary showed a sharp decrease in packing density and larger somata. The layer III had both pyramidal and nonpyramidal neurons the somatic area of which was 149 μm2. There was a slight increase in the 16 packing density of the small stellate cells and the chief neuronal population of layer IV. The average somatic area of layer IV was 111 μm2 which was much smaller than that of cells in layers III or V. Layer V had a lower neuronal packing density and large cell rise than layer IV. The large pyramidal cells were more numerous in the layer (Vb) than in the layer (Va). The average somatic area of layer V was 182 μm2. Layer VI contained closely packed, flattened neural somata with an average perikaryal area of 122 μm2. They concluded that the data would serve as a basis for the subsequent identification of experimentally labeled cells on the basis of their somatic shape and dendritic profiles. Krimer et al. (1997) studied enterorrhinal cortex in 14 postmortem schizophrenic brains and 14 matched controls. They noted that total neuronal numbers in the schizophrenic group compared with controls were: 10185 ± 1730 versus 10105 ± 3144 in Ir, 6923 ± 2276 versus 6631 ± 1575 in Ic and 7303 ± 1125 versus 7746 ± 1330 in S. They found pyramidal shaped neurons in third layer of cerebral cortex in schizophrenic patients. They concluded that mild quantitative abnormalities might exist in the ERCr and might possibly be revealed in a larger sample of schizophrenic brains. Peinado et al. (1997) evaluated the quantitative and cytomorphometric effects of aging on neuronal and glial populations in cortex of the rat. They recorded cortical volume, neuronal density, glial density, and neuronal area, and shapes of the soma and nucleus in cortical layers I, II–IV, V, and VI using serial sections stained with cresyl-fast violet. They found age-related decrease in the area of the neuronal soma in layers II–IV, V, and VI. They concluded that the stability of neuronal density together with the increased number of glial cells and the changes in neuronal soma size was suggestive of aged-related cognitive impairment which had a consequence of neuronal dysfunction rather than actual neuronal losses. Zecevic and Rakic (2001) studied the layer I of the cerebral cortex and found that, in the macaque monkey, neurons of this layer were generated during the entire 2 month period of corticogenesis. The large, classical Cajal-Retzius cells were generated first and that processes of these cells form a stereotyped, rectangular network oriented parallel to the pial surface. Rabinowicz et al. (2002) investigated gender differences in size of neuronal somata. They reported that the neuronal soma size was found to be 17 838.7±10.5 μm3 in case of male while in female it was 871.2±14.9 μm 3. The female group showed significantly larger neuropil volumes than males, whereas neuronal soma size and astrocytic volumes did not differ. The expanded data confirmed higher neuronal densities in males than in females without a gender difference in cortical thickness. Srivastava et al. (2009) studied cyto-architecture and morphology of the neuronal types of the dorsomedial cortex of the lizard, Hemidactylus flaviviridis. Bitufted neurons had vertically arranged fusiform somata, which were long, slender, or short thick (20 x 9 μm, mean size), but they always had their cell bodies located in the center of the cell layer. Pyramidal neurons had conical to pyramidal shaped somata (19 x 13 μm, mean size) situated in the inner rim of the cell layer. The length of soma ranged from 15 to 23 μm while width ranged from 10 to 16 μm. Inverted pyramidal neurons had conical to pyramidal shaped somata (21 x 12 μm, mean size), whose apical dendrite oriented towards the inner plexiform layer. The length of soma ranged from 13 to 30 μm while width ranged from 8 to 12 μm. Bipyramidal neurons had biconical or bipyramidal shape cell body (20 x 12 μm, mean size), whose length varied from 14 to 26 μm and width ranged from 6 to 15 μm. Multipolar neurons had polygonal or ovoid cell bodies (19 x 12 μm, mean size). The length of soma ranged from 15 to 25 μm while width ranged from 10 to 16 μm. Sur et al. (2011) compared characteristics of silver stained nucleolus of the Purkinje cells and structural differences of the cortex, in turkeys, ducks, pigeons, and starlings. The thickness of the molecular and granular layer at the summit of the folia was the highest in pigeons, whereas the highest value of the molecular and granular layer was determined at the deep of the folia in turkeys. The highest number of Purkinje cells per unit scale was observed in pigeons and starlings. The mean sizes of the Purkinje cells were greater in turkeys than in other species. The mean area of the Purkinje cell nucleus and counts were found to be higher in turkeys. However, there was no difference in the mean ratio of Purkinje cell area to Purkinje cell nucleolus area among the species. They concluded that the results obtained from this study could be of particular interest to comparative biologists and physiologists. 18 2.5. Cortex width Shefer (1972) studied thickness of the cortex in areas 6, 10, 18, 21, 21/38 and 40 and in the healthy persons of different ages and in patients with senile and vascular dementia with Pick's or Alzheimer's disease. In old age, the mean thickness of the cortex on the free surface of the gyri in healthy persons found unchanged. In persons with senile and vascular dementia, there was no decrease in the thickness of the cortex on its free surface. In Alzheimer's disease, the thickness of the cortex reduced by 6%. Pick's disease was characterized by mass death of nerve cells in the affected areas, leading to decrease in thickness of the cortex by half. The subicular cortex thickness in old age was reduced by 28 % and in diseases leading to dementia by 47-71%. Games and Winer (1988) studied adult male albino rats. Layer I had few neurons and extended some 140 μm below the pial surface. It formed 13% of the total thickness of the cortex (averaging 1100 μm). Layer II was 125 μm thick, represented 11% of the cortical thickness. Layer III was 190 μm thick and formed 17% of the cortical thickness. Layer IV was 105 μm thick, represented 10% of the cortical depth. Layer V was 270 μm thick, formed 26% of the cortical thickness. Layer VI was 245 μm thick, represented 22% of the cortical depth. Witelson et al. (1995) analysed of nine brain from five men and four women. They found that the average cortical depth did not differ between the sexes for either total cortex or for any layer. There was a tendency for layer III to be greater in men (902 µm) than women (810 µm) by 11%. In contrast, layers II and IV showed virtually no difference between the sexes. The total cortical width was found to be 2735 µm in women and 2923 µm in men. The minimum cortical width was found to be 275 µm in women and 277 µm in men in cortical layer II. Semendeferi et al. (2001) studied values for the relative width of the supragranular, granular, and infragranular layers. In the human, layer I had a mean value of 11% of the total cortical depth, layers II and III make up 43% of the cortical thickness and infragranular layers V and VI was found to constitute 40%. Layer IV showed to make up 6% of the total depth. The size of the infragranular layers was almost half the total thickness of the cortex of the frontal pole, while the size of layers II and III was reduced. With regard to the gibbon and the macaque, the values were similar, with the exception of layer IV, which was much wider in the gibbon brain (11% vs. 6% in the macaque). 19 Rabinowicz et al. (2002) investigated gender differences in cortical thickness. The histo-morphometric study included brains of 6 males and 5 females, 12 to 24 yr old. The cortical thickness was found to be 2.580± 0.036 mm in case of male, while in female it was 2.656±0.034 mm. The female group showed significantly larger neuropil volumes than males, whereas neuronal soma size and astrocytic volumes did not differ. The expanded data confirmed higher neuronal densities in males than in females without a gender difference in cortical thickness. Cullen et al. (2006) suggested that neuronal density in left dorsolateral prefrontal cortex was increased in schizophrenia. They estimated neuronal density, size and shape in the prefrontal cortex of the left and right hemispheres of brains. They found marked decrease in layer 3 thickness. This loss or reversal of asymmetry was most significant in cortical layer 3. Pyramidal neurons in this cell layer were significantly larger on the left and more spherical in shape than on the right side in control brains, but size and shape did not differ between the two sides in schizophrenia. Altamura et al. (2007) assessed the thickness and neuronal cell density of various cerebral cortical areas in mice. The thickness of layer IV was decreased in aged mice. Overall cortical thickness was decreased in many cortical areas of 5-HTT ko mice with increased in supragranular and infragranular layers, which compensate entirely for decreased layer IV thickness, resulting in unchanged or even enhanced cortical thickness. Srivastava et al. (2009) studied cyto-architecture and morphology of the neuronal types of the dorsomedial cortex of the lizard & Hemidactylus flaviviridis. They recorded three neuronal layers in dorsomedial cerebral cortex. The thickness of outermost layer-I ranged from 28-210 μm. This layer had only few neuronal somas and also the dendrites which were ascending from subjacent layer-II. The Layer-II was characterized by densely packed neuronal cell bodies. It also contained dendrites descending from outer layer-I and ascending from inner layer-III. The thickness of layer-II ranged from 19-93 μm. Layer-III was 28269 μm thick and had loosely packed neuronal cell bodies. It also contained dendrites descending from layer-I and II and ascending processes from ependymal layer. The ependymal layer was observed in all the cortical areas except the lateral cortex. 20 Sur et al. (2011) compared characteristics of silver stained nucleolus of the Purkinje cells and structural differences of the cortex, in turkeys, ducks, pigeons, and starlings. The thickness of the molecular and granular layer at the summit of the folia was the highest in pigeons, whereas the highest value of the molecular and granular layer was determined at the deep of the folia in turkeys. They concluded that the results obtained from this study could be of particular interest to comparative biologists and physiologists. Smiley et al. (2012) measured neuron density and width of the cortex in planum temporal and also in prefrontal area 9 of the same brains. They reported smaller volume and width of the outer cortex (layers I-III) in bilateral planum temporal in the left hemisphere of schizophrenic patients. There was no significant effect of schizophrenia on neuronal density or width of cortex in both the cortical regions. 2.6. Histochemistry and histoenzymology Manocha (1970) studied distribution of some phosphatases (alkaline and acid phosphatase and ATPase) in the various regions and nuclei of the aged squirrel monkey brain. The alkaline phosphatase activity was concentrated in the blood vessels and the peripheral part of the neurons of cerebrum and cerebellum. Acid phosphatase (AC) was concentrated in the pyramidal cells of cerebral cortex and the Purkinje cells of cerebellar cortex. They concluded that AC activity was more related to static maintenance metabolism of cells than to dynamic functional metabolism. Sohal and Sharma (1972) compared fine structure and population density of neurons in the brains of young and senescent male houseflies to elucidate the role of the nervous system in the physiological aging of multicellular organisms. Statistical comparisons of neuronal populations in 3 day old flies with those of 30 day old flies showed no significant difference in the number of neurons. Neurons of senescent flies showed several significant deteriorative changes in their fine structure including loss of ribosomes, focal cytoplasmic degeneration, autophagy and accumulation of acid-phosphatase-positive dense residual bodies. The observations made in this study suggest that the age-related impairment in function of the nervous system may be due to intraneuronal alterations rather than to the loss of nerve cells. 21 Sood and Mulchandani (1977) studied the histochemical mapping of the distribution of acid and alkaline phosphatases and succinic dehydrogenase in the medulla oblongata and spinal cord of mouse. The acid phosphatase was observed in neurons of all the nuclei with activity varying from intense to moderate. In alkaline phosphatase preparations most of the nuclei were either intensely positive or moderately positive. In case of succinic dehydrogenase out of 36 nuclei studied, 9 nuclei were intensely positive, 11 areas were moderately positive, 7 were mildly positive and 9 were completely devoid of enzymatic activity. They correlated this distribution of the above enzyme with the functional significance of the various nuclei. Hafiza and Sood (1979) studied the distribution of alkaline phosphatase and 5-nucleotidase in the medulla oblongata of Taphozous melanopogon (Bat). They found that the activity of alkaline phosphatase was stronger in neurons than in neuropil and that of 5-nucleotidase was stronger in neuropil than in neurons. Blood capillaries were completely negative for alkaline phosphatase and intensely positive for 5-nucleotidase. Mohanakumar and Sood (1979) comparatively studied the acid and alkaline phosphatases within the medulla oblongata and pons of the hedgehog (Paraechinus micropus). They stated that the cellular elements were positive for acid phosphatase, irrespective of their sensory or motor nature and the large neurons of all the nuclei were more strongly positive than the small cells. Furthermore, the nuclei which contain a dense population of neurons, appeared more strongly positive than areas containing scattered neurons. Alkaline phosphatase preparations showed a strong activity in the walls of blood capillaries of the medulla oblongata and pons. Reaction of this enzyme in the neuropil varies from moderate to strong. Sood and Sinha (1983) studied distribution of alkaline phosphatase (GPAII) and acetylcholinesterase (CHO-A) in the hind brain of Channa punctatus and Heteropneustes. They stated that CHO-A was localized only in the neurons. The motor neurons show a stronger staining than the sensory neurons. In Heteropneustes the sensory nuclei of the VIIth and Xth nerve are better developed and show stronger activity of CHO-A than Channa. In Channa, motor nuclei were better developed and show stronger activity of CHO-A than in Heteropneustes. Irrespective of their sensory or motor nature, all the cranial 22 nerve nuclei exhibit a strong GP-AII activity. Purkinje cells of Heteropneustes showed stronger activity of GP-AII than Channa. Khan and Sood (1984) studied histoenzymological architecture and location of phosphatases (acid and alkaline phosphatases, 5-nucleotidase, adenosine triphosphatase) of the rhombencephalon and mesencephalon of a fresh water turtle. They reported that concentration of acid phosphatase was higher in large neurons. Alkaline phosphatase predominates in blood vessels. Neuropil and neuronal activity of this enzyme was restricted to limited nuclei, only. 5-nucleotidase was localized in all the cells as well as in the neuropil. Adenosine triphosphatase activity was quite strong in all the brain areas irrespective of their sensory and motor nature. In turtle brain it was not been possible to distinguish sensory and motor areas on the basis of phosphatases distribution as in fishes and mammals by several workers. Ng and Tam (1986) studied agewise changes in acid and alkaline phosphatase activities of cerebrum, cerebellum and brainstem in mouse. Acid and alkaline phosphatase activities in the mouse cerebrum, brainstem and cerebellum increased during the late fetal and perinatal period and reached a maximum on postnatal day 28 (P28). Myelination was most extensive on P28– P45 in the brainstem and in the cerebellum, and phosphatidic acid phosphatase activity concomitantly decreased to a low level after birth. They correlated the changes in acid and alkaline phosphatase activities with the extent of myelination than with the overall brain tissue growth. Nakamura et al. (1989) studied age-dependent change in activities of seven lysosomal enzymes was studied in four brain regions (cerebrum, hippocampus, pons and cerebellum) of Wistar rats. The activity of cathepsin D was significantly increased with aging in the four regions. The age-dependent change in activities of acid and alkaline DNases showed the characteristic regional difference, and the ratio of acid to alkaline DNases was increased with aging in all regions. Acid RNase showed the lowest activity in 18-month-old rats, and alkaline RNase activity was decreased with aging. Acid phophatase showed no significant age-dependent change except in pons. They demonstrated that all of the lysosomal enzyme activities do not change in parallel with aging and the age-dependent change showed the characteristic regional difference. 23 Capucchio et al. (2010) studied systematically characterize lesions in the brains of 60 horses aged from 7 to 23 years. They reported that the PAS positive activity increases with age in horse brain. Lipofuscin storage chiefly affected the large cortical neurons and numerous neurons of the brainstem nuclei in 57 horses (95%). Lipofuscin was observed mostly in the perikaryon, between the nucleus and the axon hillock. They could not found lipofuscin pigment in the brains of the young control animals. Gerhauser et al. (2012) studied 520 Syrian golden hamsters to detect background lesions observed in the CNS. They reported PAS positive activity in few large neurons in cerebellum but they did not find any agewise difference. Hamsters (92.1%) of the oldest age group showed a mild lipofuscinosis characterised by LFB and PAS positivity in few large neurons. These cells were localised predominantly in the spinal cord ventral horns, the medulla oblongata including the facial and cochlear nuclei. Kellett et al. (2011) investigated Memory and Aging cohort (121 AD patients, 89 mild cognitive impairment (MCI) patients and 180 control subjects) in human brain. They reported increasing alkaline phosphatase activity. Alkaline phosphatase was present on neuronal membranes that reflect the neuronal loss. Alkaline phosphatase activity was significantly higher in the AD patients relative to the controls. Plasma alkaline phosphatase activity inversely correlated with cognitive function in controls, MCI and AD patients. These data indicated that plasma alkaline phosphatase activity was increased in AD and inversely correlates with cognitive function. Vardy et al. (2012) measured the activity of alkaline phosphatase in human brain in Alzheimer‟s disease and appropriate age-matched controls, and in an ageing series of brains. In addition, they also measured TNAP activity in plasma from 110 AD and 110 non-demented control participants. They reported that there was no increase in alkaline phosphatase activity with the advancement of age, but the activity was found increased in brain of Alzheimer‟s disease. Manich et al. (2016) studied age related changes in hippocampus, piriform, entorhina and cerebral cortices of mice brain. They reported progressive appearance and expansion of degenerative granular structures frequently referred as "PAS granules" because of their positive staining with periodic acidSchiff (PAS). They reported the presence of a neo-epitope in mice hippocampal 24 PAS granules and the existence of natural IgM auto-antibodies directed against the neo-epitope in the plasma of the animals. They suggested that neo-epitopes may turn into a useful brain-ageing biomarker and that autoimmunity could become a new focus in the study of age-related degenerative processes. 2.7. Lipofuschin pigment Whiteford (1964) conducted an experiment to determine the occurrence, distribution, and significance of lipofuscin in the central nervous systems of the dog and pig. Tissue blocks representing the medulla oblongata, pons, cerebellum, mesencephalon, and diencephalon were embedded in paraffin, sectioned and stained for lipofuscin pigment. He reported that lipofuscin was widely distributed throughout the central nervous systems of the aged canine and porcine specimens. The intracytoplasmlc pigment granules were seen varied in pattern of distribution with advancement of age than to the functional type of neuron in which it occurred. He concluded that the amount of pigment contained within the individual cell, and the number of cells containing pigment increased with age and appeared to be independent of breed and sex. Heinsen (1981) studied the distribution of lipofuscin in the perikarya of Purkinje cells of vermal and hemispheric lobules in 7 rats of 30-38 months old, by the point-counting method. He found that the Purkinje cells of lobule VIa were extremely lipofuscin-rich. The Purkinje cells of the hemispheres, lobules V, Vlb+c and VII contain considerable amounts of a finely granular lipofuscin while lobules I-III and VIII- IXa contain globular type. The Purkinje cells of sublobule XI d c and X were lipofuscin-poor cells. The Purkinje cells immediately rostral and caudal to the primary fissure were rich in lipofuscin and there was 2 to 2½ times as much lipofuscin in these cells as in the Purkinje cells of lobules X and IX d c. The Purkinje cells in lobules II-IV were found to accumulate increasing amounts of lipofuscin as one approaches the primary fissure. He concluded that the Purkinje cells of lobule I and IX were not fit into this pattern, because the cells in lobule I had more lipofuscin than the cells in lobule II. Nandy (1981) studied cerebellar cortices in 4, 10 and 20 year of Macaca nemestrina for the number of Purkinje (P) and granule cells and the deposition of lipofuscin in P cells in relation to aging. Lipofuscin distribution significantly increased within the P cells in these animals. The number of P cells was 25 significantly reduced, while there were no changes in the number of granule cells. It appears from this and other studies that the Purkinje cells are more prone to aging changes than the granule cells of the cerebellum both in lipofuscin formation and cell loss. Although the precise functional significance of these changes in P cells is not clear, their vulnerability may be related to changes in motor function in old age. Newsholme et al. (1985) studied paresis afflicted 85 Merino ewes kept on old wheat lands in the Western Cape, where vegetation was sparse but dominated by Trachyandra divaricata. They found abundant, yellowish-brown pigment granules in the cytoplasm of most of the larger neurons and in some non-nervous tissues. Shrinkage and loss of a few randomly scattered axons were observed in the white matter of the spinal cord in 2 sheep. Histochemical and ultrastructural features of the pigment were consistent with those of lipofuscin. They concluded that the Trachyandra poisoning appears to be the first documented example in farm animals of an acquired lipofuscin storage disease involving nervous and non-nervous tissues. Sturrock (1989) carried quantitative histological study sections of brains from mice upto 31 months of age. The average diameter of large neurons in the lateral vestibular nucleus was 12.2±0.2 µm at 25 months of age which increased to 14.3±0.2 at 31 months. The average diameter of small neurons was 11.02±0.2 µm at 25 months of age, which was 10.9±0.1 at 31 months. Large neurons were found to accumulate lipofuscin from 25 months and their mean nuclear diameter increased significantly between 25 and 28 months of age. Small neurons showed very little lipofuscin even at 31 months of age and their mean nuclear diameter remained constant between 6 and 31 months. Tigges et al. (1990) studied age related changes in neuronal population of area 4 of 19 rhesus monkeys, ranging in age from 1 day to 35 years. They found that lipofuscin granules were dissemble in Betz cells beginning at the age of 5 years and their number increased with increasing age. In the older rhesus monkeys, the lipofuscin granules were so large and numerous that in some Betz cell soma, they displaced the nucleus from its usual location in the center of the cell. They found no age-related change in thickness of area 4. Monteiro (1991) studied quantitative age related changes occurring in somatic organelles of the neocerebellar Purkinje cells using female rats aged 2 to 26 24 months. He calculated the somatic volumes and volumetric fractions. He stated that the dense bodies having lipofuscin were found the display the most expressive ultrastructural age changes. Sharma and Singh (1994) examined the alteration in the number of lipofuscin containing neurons in the parietal cortex of brain rat at 4,8,16 and 24 months of age. They noted in the neuron. They found increase in number of neurons with lipofuscin with advancement of age. Similarly they observed decreased PAS positive neurons and increased NBS positive neurons with advancement of age. The statistical interrelations between the neuron changes indicated that the scattered lipofuscin gets aggregated with the advancement of age. Mochizuki et al. (1995) compared the autofluorescence features of lipofuscin in the brain and adrenal of rat. They reported that in 18 -21 months old rats, the brain lipofuscin was granular and its autofluorescence was bright whitish yellow to bright orange. On the contrary, the adrenal lipofuscin was not demarcated as granules, and its autofluorescence was subdued orange. They concluded that the present results showed that the autofluorescence features of the bright whitish yellow brain lipofuscin and the adrenal lipofuscin were quite different. Borras et al. (1999) compared brains of 20 old dogs, ranging in age from 8 to 18 years, with those of 10 young dogs using routine staining techniques. They noted age-related changes with respect to lipofuscin, polyglucosan bodies, and β-amyloid. Narrowing of gyri and widening of sulci were also evident in some of the oldest dogs. They recorded mild to moderate ventricular enlargement in aged animals. Meningeal calcification was observed in dog‟s dorsal hemispheric leptomeninges and concentric basophilic structures resembling plammoma bodies. They observed small localized foci of satellitosis and neuronophagia in 10 dogs (50%) particularly in frontal cerebral cortex and dorsal thalamus section which was supposed to play a role in the pathogenesis of the canine cognitive dysfunction syndrome. Gilissen et al. (1999) compared aged dwarf (Cheirogaleus medius) and mouse (Microcebus murinus) lemurs by autofluorescence for lipofuscin and iron distribution in brain sections. Lipofuscin accumulation was observed in the aged animals, but not in the young ones. Affected regions where no iron accumulation 27 was observed include the hippocampus (granular and pyramidal cells), the olfactory nucleus and the olfactory bulb (mitral cells), the basal forebrain, the hypothalamus, the cerebellum (Purkinje cells), the neocortex (essentially in the pyramidal cells), and the brainstem. They concluded that the different biochemical and morphological cellular compartments might be involved in iron and lipofuscin deposition. The nonuniform distribution of lipofuscin indicated that brain structures were not equally sensitive to the factors causing lipofuscin accumulation. Vila et al. (2000) carried out quantitative study of the lipofuscin content by image analysis in brains of known-age, pond-reared prawns Penaeus japonicas. Three distinct measurements of lipofuscin levels (% area fraction, granule density and mean granule size) were recorded in ten sections of the olfactory lobe cell mass (OLCM) per animal. The concentration of lipofuscin increased significantly with age and was independent of sex. The relationship between age and lipofuscin concentration was best described by a seasonalized von Bertalanffy function, since the accumulation rate of the pigment dramatically slowed down in fall-winter, and ascribed it to a result of reduced seasonal metabolism. They confirmed the potential of the lipofuscin method in the estimation of physiological age in penaeids and suggested that the application of this methodology could be useful in studies of age structure in wild populations. Sobrino et al. (2001) developed a new method on basis of the gradual deposition of lipofuscin in post-mitotic tissues for calculating age of animals. The identification of the age-pigment realized on transversal sections of the olfactory lobe cells mass in brain of shrimp (Aresteus antennatus). Three different measurements of lipofuscin levels (%area fraction, granule density and granule mean size) were recorded in ten distinct sections. Relationship between body size and lipofuscin concentration (% area fraction and density granule) increased significantly. They found that the age calculation from the lipofuscin distributions was more successful than for body size distribution. Growth parameters using Von Bertalanffy function were derived for this species on basis of the lipofuscin. They confirmed the potential of the lipofuscin method for the resolution of cohorts in deep-water pennies and suggested that the method could be useful in studies of the age structure in wild populations. 28 Nesic et al. (2013) studied brain samples from 59 dogs from four groups according to age ( group A, up to 5 years; group B, 5-10 years; group C, 10-15 years; and group D, >15 years). Parts of the brain (frontal cortex, parietal cortex, hippocampus, cerebellum and medulla oblongata) were stained with haematoxylin and eosin, periodic acid Schiff and Ziehl Neelsen techniques. They found that the pigment was detected in 80% of the dogs in group D, while this percentage was 30% in group A. In groups A and B, lipofuscin accumulated only in neurons of the medulla oblongata. In group C and D lipofuscin was detected in various percentages in neurons of all brain sections. They concluded that the lipofuscin was found mostly accumulated in large neurons of the nuclei of the medulla oblongata. The accumulation of lipofuscin pigment in neurons increased with the age and progressively involved neurons of different brain regions. 2.8. Electron microscopy: Vincent et al. (1989) studied light and electron microscopic examination of cerebral cortex in well-fixed young (5-6 years) and old (25-35 years) rhesus monkeys to determine the effects of age on neurons. They found that in old monkeys the neurons showed little cytological evidence of advanced age beyond the presence of a few lipofuscin granules, although the neuropil contains some profiles of degenerating small-caliber dendrites, myelinated axons, and a few axon terminals. Large vacuoles, some 10 microns or more in diameter, were present in the neuropil of the old animals. Some of these vacuoles appeared to represent a late stage in the degeneration of myelinated axons, for that they were bounded by a thin, laminated sheath. Other large vacuoles, of unknown origin, often contain membranous debris and had an attenuated limiting membrane. They concluded that the cell bodies of neurons in area 17 of old rhesus monkeys did not show significant structural changes due to age, although some of the neuronal processes in the neuropil were affected. Peters et al. (2000) studied myelin sheaths in young and old monkeys. They reported local splitting of the major dense line to accommodate dense cytoplasm derived from the oligodendrocytes. Other alterations were the formation of redundant myelin so that formation of double sheaths, in which one layer of compact myelin was surrounded by another one. These alterations in myelin increase in frequency with the ages of the monkeys, and there was a 29 significant correlation between the breakdown of the myelin and the impairments in cognition exhibited by individual monkeys. They suggested that the breakdown of myelin could impair cognition by leading to a change in the conduction rates along axons, resulting in a loss of synchrony in cortical neuronal circuits. Peters (2002) stated that human and non-human primates showed cognitive decline during normal aging. He stated that some myelin sheath exhibit degenerative changes such as splitting of myelin sheath and formation of balloons with the advancement of age. He further suggested that such degenerative changes lead to cognitive decline since they cause change in conduction velocity, which results in dysfunction of normal timing in neuronal circuits. Neurons were found to acquire pigment, but the only other obvious changes were in pyramidal cells lose branches, as well as synapses, and at the same time the glial limiting membrane was found thickened. That might be contributing to cognitive decline because it would cause a slowing of conduction along nerve fibers and disrupting the timing in neuronal circuits. 30 3. MATERIALS AND METHODS Collection of samples: The present study was conducted on the samples of cerebrum, cerebellum, pons and medulla oblongata of brain from 20 goats irrespective of sex. The Group-I comprised of goats between 9 to 12 months of age and GroupII comprised of goats of 13 months and above age. The brain samples were procured from the slaughter house, Municipal Corporation and Ahillyabai Holkar sheli-mendhi Vikas Mahamandal Bondri, immediately after the slaughter. The fresh samples thus collected were brought to laboratory in the container containing ice cubes. Samples were preserved in 10% neutral buffered formalin for 48 hours for routine processing by paraffin technique. Unfixed fresh samples were also utilized for cryostat sectioning. Gross observations: The biometrical / morphometrical observations of the brain were recorded as follows. 1. Weight of the brain (Wt): The weight of brain on digital balance before treating with 10% neutral buffered formalin for 48 hours. The measurements were recorded in Gram (gm). 2. Length of brain (LB): Distance from the most rostral point of the olfactory bulb to the medulla oblongata, recorded in centimetres (cm). 3. Width of brain (WB): Largest distance between lateral borders of two hemispheres, recorded in centimetres (cm). 4. Depth of brain (DB): Distance from the dorsal aspect of the cerebrum to the ventral aspect of the cerebrum, recorded in centimetres (cm). 5. Length of cerebrum (LC): Distance from the rostral pole of the cerebrum to the caudal pole, caudal to the occipital gyrus, recorded in centimetres (cm). 31 6. Width of cerebrum (WC): Largest distance between lateral borders of two cerebral hemispheres, recorded in centimetres (cm). 7. Depth of cerebrum (DC): Distance from the dorsal aspect of the cerebrum to the ventral aspect of the cerebrum, recorded in centimetres (cm). 8. Length of cerebellum (LCB): Distance from the most rostral end of the cerebellum where it is in contact with the cerebrum, to the most caudal point or far extremity, rostral to the medulla oblongata, recorded in centimetres (cm). 9. Width of cerebellum (WCB): Widest distance between two lateral lobes, recorded in centimeters (cm). 10. Depth of cerebellum (DCB): Distance from the highest point of the median vermis to the roof of the fourth ventricle, recorded in centimetres (cm). 11. Brain Index= (length of brain x 100) / width of brain. 12. Cerebral Index = (length of cerebrum x 100) / width of cerebrum. 13. Cerebellar Index = (Width of cerebellum x 100) / length of cerebellum. Processing and preparing samples for Histological study: The samples were fixed in 10% neutral buffered formalin for 48 hours at room temperature. After fixation, the tissues were washed in running tap water for overnight, dehydrated in ascending grades of alcohol (70%, 80%, 90%, 95% and absolute alcohol), cleared in xyline, infiltrated in three changes of melted paraffin at 600 C for 4 hours and embedded in fresh melted paraffin by employing manual tissue schedule as per the method of Drury and Wallington (1980). The paraffin blocks were prepared and stored in refrigerator. Sections were cut at ten micron thickness with help of rotary microtome, mounted on glass slides, dried at 370 C and preserved carefully for different staining as follows. 1. The Haematoxylin and Eosin staining methods as per Drury and Wallington (1980). 2. Toluidine blue as per Bancroft & Stevens (1982) 32 3. Luxol fast blue as per Bancroft & Stevens (1982) 4. Silver impregnation method as per Bancroft & Stevens (1982) The stained slides were observed under light microscope to study the histoarchitecture of the cerebrum, cerebellum, pons and medulla oblongata. Micrometrical parameters: The following micrometrical parameters were carried out for the various components of the brain and recorded in micrometer (µm) as per the method of Culling (1969). The micrometrical parameters were recorded by calculating averages of 3 to 4 fields for each prepared slides. 1. The Neuron numbers per square mm in cerebrum at different lobes.(Frontal, temporal and occipital lobes) 2. The Neuron numbers per square mm in cerebrum at different layers. 3. Thickness of cerebral cortex (µm). 4. Thickness of each layers of cerebral cortex (µm). 5. Cell packing density for total cerebral cortex (per cubic mm). 6. Cell packing density for each cerebral layer (per cubic mm). 7. The Neuron numbers in cerebellum at different layers (per square mm). 8. Thickness of each layers of cerebellar cortex (µm). 9. Cell packing density for each cerebellar layer (per cubic mm). 10. The Neuron numbers in pons (per square mm). 11. Cell packing density for pons (per cubic mm). 12. The Neuron numbers in medulla oblongata (per square mm). 13. Cell packing density for medulla oblongata (per cubic mm). All the measurements recorded were analysed statistically as per the standard method given by Snedecor and Cochran (1994). Finally, the slides were microphotographed. 1. The Neuron numbers in cerebrum and cerebellum in different layers. 33 The method used for neuronal count was based on a computer assisted manual method involving cell differentiation in Nissl-stained sections under the microscope, marking neurons and defining layer thickness using photomicrography. Low power photomicrographs of the sites chosen for measurement for each slide were used to delineate the six cortical layers. The number of neurons was counted for each layer from the tracings with the aid of a computer. This neuron count is used for calculating the neuron density by using following formula (Witelson et al. 1995). Nv = n/(d x w x t) Where, Nv = the number of neurons per 1 mm3 of cortical tissue (cell packing density) n = the mean number of neurons from two traverses, d = the mean cortical or laminar depth of the two traverses (in mm) w = the width of the traverse (in mm) t = the mean section thickness (in mm). Number of neurons under 1 mm2 of cortical surface for each layer Nc = n/(wxt), Where Nc = the number of neurons under 1 mm2 of cortical surface, n = the mean number of neurons from two traverses, w = the width of the traverse (in mm), t = the mean section thickness (in mm). 2. Thickness of cerebral and cerebellar cortex Thickness of cerebral and cerebellar cortex was calculated by photomicrography using Haematoxylin and Eosin staining method and Toludine blue staining method. 34 Processing and preparing samples for histochemical study: Fresh tissue samples were cut at 10 µ thickness on cryostat and stained for glycogen, lipid, alkaline phosphatase and acid phosphatase by staining with the following procedures: 1. Periodic Acid Schiff‟s (PAS) reaction method for glycogen (Singh and Sulochana,1996). 2. Standard Sudan Black B method for lipids (Singh and Sulochana,1996). 3. Acid phosphatase activity as per Bancroft & Stevens (1982). 4. Alkaline phosphatase activity as per Bancroft & Stevens (1982). Transmission Electron Microscopy (TEM) Protocol : Samples were fixed in 2.5% gluteraldehyde in 0.1 M phosphate buffer (pH 7.2) for 24 hrs at 40c and washed with PBS for 2 times each 45 minutes, then post fixed in 1% aqueous Osmium Tetroxide for 2 hrs later washed with deionised distilled water for 4 times each 45 minutes, dehydrated in series of graded alcohols, infiltrated and embedded in araldite 6005 resin or spur resin (Spurr 1969). Incubated at 800C for 48 hrs for complete polymerization. Ultra thin (60 nm) sections were made with a glass knife on ultra microtone (Lieca Ultra cut UCT-GA-D/E-1/00), mounted on copper grids and stained with saturated aqueous Uranyl acetate (UA) and counter stained with Reynolds lead citrate (LC). Viewed under TEM (Model: Hitachi, H-7500 from Japan) at required magnifications as per standard procedure at RUSKA Lab‟s College of Veterinary Sciences, SPVNRTSUVAFS, Rajendranagar, Hyderabad, India. 35 4. RESULTS AND DISCUSSION In the present study the fresh samples of goat brain were collected on ice. The biometrical observations of whole brain were recorded. Pieces of cerebrum, cerebellum, pons and medulla oblongata were cut separately for histological, histochemical, histoenzymic and electron microscopic study. The general histological structures of these parts of brain were studied from the sections stained with hematoxylin and eosin method. Various tissue components were studied from the sections stained with Toluidine blue, Gallocyanin, Luxal fast and silver impregnation stains. Age wise histochemical and histoenzymic changes in the cerebrum, cerebellum, pons and medulla oblongata were observed by performing PAS reaction for glycogen, Azo dye coupling method for acid phosphatase and alkaline phosphatase and lipofuscin deposited by Sudan black B. Age wise changes pertaining to myelin degeneration, pigment deposition and mitochondrial changes if any, were studied by Electron microscopic method. 4.1. Biometrical observations: The gross morphology indicated that the cerebrum of goat is oblong to oval in shape. The two hemispheres were separated by a mid sagittal cleft. The olfactory lobe was blunt. The gyri and sulci were prominent. The gyri were fine and sulci were deeper. The cerebellum was oval with wider capacity along transverse axis. Because of longer width the cerebellum appears more globular in shape. Pons was transversely elongated structure located on ventral aspect of brain stem with faint median furrow. While the medulla oblongata was distal continuation of brain and continued as spinal cord. It was dorsolaterally flattened cord like structure. The different measurements of various components of brain are recorded as follows. 4.1.1. Whole brain: Weight of the brain in Group I was recorded as 120.30±2.85 gm which ranged between 104 gm to 134 gm while in Group II, it ranged between 125 gm to 140 gm with mean value of 131.60±1.75 gm (Table 1). A highly significant difference was observed in the weight of whole brain in Group I and Group II with the advancement of age. In agreement with the present findings, Olopade et al. 36 (2011) in Nigerian pig and Olopade et al. (2005) in African dwarf sheep reported positive correlation between the weight of brain and weight of head. However, this observation of the present study is not in accordance with that reported by Byanet et al. (2008). They stated that there is no proportional increase in the brain weight with increase in body weight in African grasscutter. Kigir et al. (2010) also reported that there was no significant difference between weights of brain with the advancement of age in Sahel goat. Olopade et al. (2007) reported that the weight of brain in the Nigerian goats were 85.13 gm in Sahel, 85.85 gm in Red Sokoto and 56.89 gm in the West African Dwarf goat. These values were much lesser than the current findings, this variation in weight of brain might be due to breed difference. The variation in the weight of the whole brain in Group I and Group II with the advancement of age found during present study might be attributed to the fact that brain is the site for deposition of fat which is more during the advanced age or might be due to proliferation of neuronal supporting tissuemicroglia. The Length of whole brain in Group I ranged between 9.20 cm to 10.80 cm with mean value of 9.91±0.15 cm and in Group II, it ranged between 9.20 cm to 10.60 cm with mean value of 9.77±0.13 cm. The Width of the brain in Group I ranged between 5.6 cm to 6.9 cm with mean value of 6.33±0.16 cm and for Group II, it ranged between 5.6 cm to 6.7 cm with mean value of 6.23±0.10 cm. Depth of the brain in Group I ranged between 3.7 cm to 4.3 cm with mean value of 3.98±0.06 cm and for Group II, it ranged between 3.6 cm to 4.4 cm with mean value of 3.96±0.09 cm. These observations of the present study showed that there was linear decrease in the length, width and depth of brain with advancement of age. However, no significant difference in the length, width and the depth of brain was observed during present study. These observations of the present study are in accordance with those reported by Olopade et al. (2005) and Olopade et al. (2007) who reported similar decrease in measurements of whole brain in goat with advancement of age. This decrease in the measurements of various parameters of the brain could be ascribed to age associated shrinkage of neuronal tissues or elements. 37 4.1.2. Cerebrum During the present study, it was noted that the length of the cerebrum in Group I ranged between 7.60 cm to 8.50 cm with mean value of 7.82±0.15 cm and in Group II, it ranged between 7.60 cm to 8.60 cm with mean value of 8.13±0.11 cm (Table 1). Similarly the width of the cerebrum in Group I ranged between 5.10 cm to 6.90 cm with mean value of 6.04±0.18 cm and in Group II, it ranged between 5.60 cm to 6.80 cm with mean value of 6.26±0.13 cm. Although, there was no significant difference between the length and width of cerebrum with the advancement of age, but the length as well as width of cerebrum was found increased with the advancement of age. In contrast with the observations of the present study, Olopade et al. (2007) and Kigir et al. (2010) in goat reported decrease in length and width of the cerebrum with the advancement of age. Depth of the cerebrum in Group I ranged between 3.70 cm to 4.30 cm with mean value of 3.98±0.06 cm and in Group II, it ranged between 3.60 cm to 4.40 cm with mean value of 3.96±0.09 cm. Although, the depth of cerebrum showed slight decrease from Group I to Group II, but there was no significant difference in the depth of cerebrum with the advancement of age. Kigir et al. (2010) reported similar decrease in the depth of the cerebrum with the advancement of age in goat and they correlated this decrease to the fact that as the animal begin to grow, a rostrocaudal compression occurs in the brain development. 4.1.3. Cerebellum The average length of the cerebellum in Group I and Group II was recorded as 3.12±0.10 cm and 3.15±0.10 cm respectively. The average width of the cerebellum in Group I and Group II was recorded as 4.70±0.21 cm and 4.42±0.15 cm respectively (Table 1). There was no significant difference between the length and width of cerebellum with advancement of age. However, depth of the cerebellum in Group I was recorded as 3.01±0.05 cm and in Group II, it was 3.11±0.06 cm. These values indicated that there was increase in depth of cerebellum with the advancement of age. This might be attributed to the progressive growth of brain tissues. These findings of the present study are in agreement with those reported by Olopade et al. (2007) in Sahel goat. They 38 stated that this occurrence of increase in length and depth of the cerebellum with the advancement of age may translate to early independence and cognitive function for young goats. The measurement of various components of brain thus recorded were utilised for calculations of different indices such as brain index, cerebral index, cerebellar index. (Table 2). The brain index in Group I was recorded as 157.21±3.65, while in Group II it was 157.38±4.31. There was no significant difference in the brain index of Group I and Group II. Similarly, the cerebral index also did not show any significant difference between Group I and Group II. The cerebral index of the brain in Group I ranged between 104.41 to 152.94 with mean value of 130.54±4.68 and for Group II it ranged between 117.91 to 153.57 with mean value of 130.40±3.32. The cerebellar index of the brain in Group I ranged between 131.25 to 192.85 with mean value of 151.80±6.79 and for Group II it ranged between 111.76 to 161.51 with mean value of 140.79±4.27. There was no significant difference between Group I and II with respect to cerebellar index. 4.2. Histomorphology: The histomorphology of the cerebrum, cerebellum, pons and medulla oblongata was studied by staining sections with routine Haematoxylin and eosin, Toluidine blue, Gallocyanine, Luxol fast and Bielschwosky silver staining methods. 4.2.1. Cerebrum: The cerebrum was microscopically found to be made up of mainly the gray matter, which forms the cortex, while the inner part of cerebrum was made up of white matter, which was composed of myelinated axons. The grey matter externally showed the presence of very thin layer of pia matter, which was projected inside the cerebral cortex at sulci and afforded pathways for blood vessels (Fig. 1). 39 4.2.1.1. Cerebral cortex The microstructure of cerebral cortex in almost all the regions of the cerebrum viz. frontal, temporal and occipital lobes in Group I as well as in Group II, showed six layers with scattered neurons supported by varying amount of glial tissue viz. the molecular layer, the external granular layer, the external pyramidal, the internal granular, the internal pyramidal and the multiform layer (Fig. 2 to 7). The thickness of these layers was however, not uniform in all the lobes. The total cortical depth in Group I at frontal, temporal and occipital lobes was recorded as 1785.77±12.75 µm, 1745.59±11.57 µm and 1781.85±10.33 µm respectively, while in Group II, it was recorded as 1666.82±18.13 µm, 1669.10±16.20 µm and 1565.50±23.36 µm respectively. These values indicated that the cortical depth at all the three lobes under study decreased with the advancement of age. Similarly, it was also noted that there was highly significant difference in the cortical depth of the frontal, temporal and occipital lobes (table 3 to 5). In contrast with the findings of the present study, Shefer (1972) reported that thickness of the cerebral cortex remain unchanged during old age, while in Alzheimer's disease the thickness of the cerebral cortex reduced by 6%. Similarly, in Pick's disease, thickness of the cortex reduced by half. He correlated this reduction in cortical depth to mass death of nerve cells in the affected areas with the advancement of age. Tigges et al. (1990) reported no change in thickness of cortical depth of frontal lobe in monkey. In contrast with the findings of the present study regarding significant difference in cortical depth of temporal lobe with advancement of age, Smiley et al. (2012) reported that there was no significant effect of schizophrenia on neuronal density in temporal lobe with advancement of age in human. Sholl (1993) compared total cortical thickness of cerebrum in human, cat and mouse. His findings regarding total thickness of cerebral cortex in human and cat are in agreement with the present observations, but he did not report any age dependent changes in the cortical thickness. The variations in the reduction of the cortical depth at frontal, temporal and occipital lobes in the present study might be attributed to the species difference as regards to development of cerebral cortex, which is highly evolved in human beings as compared to animals. This can also be attributed to the decrease in neuronal elements along with supporting tissue with the advancement of age. 40 Three types of neurons were identified in the cerebral cortex based on the size. These neurons are categorised according to the area of the soma as small (1 to 100 µm3), medium (101 to 200 µm3) and large (more than 200 µm3) (Fig. 8, 9 and 10). Different shapes of neurons were recorded during the present study viz. spindle, pyramidal and stellate (Fig 11, 12 and 13). Similar shaped neurons were observed by Srivastava et al. (2009) in lizard and Sur et al. (2011) in turkey, duck, pigeon and starling. In Group I, the number of small neurons in frontal lobe, temporal lobe and occipital lobe were recorded as 645.4±44.29, 669.3±42.70 and 665.5±34.60 respectively; whereas in Group II, these numbers were recorded as 563.78± 52.70, 523.90± 35.20 and 531.58± 42.00 respectively. The number of medium size neurons in frontal lobe, temporal lobe and occipital lobe of Group I were recorded as 335.15±22.80, 329.22±27.90 and 285.53±24.93 respectively, whereas in Group II, these numbers were recorded as 225.55±24.80, 265.22±27.90 and 285.53±24.93 respectively. Similarly, the large sized neuron were 335.15±22.80, 329.22±27.90 and 285.53±24.93 in frontal lobe, temporal lobe and occipital lobe of Group I while in Group II, the number of neurons was 225.55±24.80, 265.22±27.90 and 285.53±24.93 respectively. The total number of neurons in frontal lobe of cerebral cortex irrespective of their size in Group I was 661.4±9.12 and in Group II, it was 612.8±8.19. The total number of neurons at temporal lobe was 687.3±15.99 and 664.8±9.73 in Group I and Group II respectively. The overall number of neurons irrespective of their size in occipital lobe was 681.5±14.79 and 615.4±5.47 in Group I and Group II respectively (Table 12). These observations regarding total number of neuron in cerebral cortex in almost all the lobes indicated that there was linear decrease with the advancement of age. These observations of the present study regarding total neuron count in frontal and temporal lobes of cerebral cortex showed highly significant difference between Group I and Group II with the advancement of age. However occipital lobe of cerebral cortex did not show any significant difference between Group I and Group II with respect to total neuron number in cerebral cortex. In agreement with the present observations, Shefer (1972) reported that the total neuron number in different areas of cerebrum decreases by 20% with the advancement of age in human. Greferath et al. (2000) reported 30% 41 reduction in number of neurons in caudal basal forebrain of female Dark Agouti rats at 26 months. In contrast to current findings Davanlou et al. (2004) reported the mean total number of cells was 128 x 106 in the cortex of the rat brain, which was found to be very high in number. Sholl (1993) reported that total numbers of neuron were about 80-60 in man and cat, which was very less in number compared to the present findings. Heinsen et al. (1994) reported that the total number of nerve cell loss was 50% in the human entorhinal areas characterized by an aging. The neuronal density was calculated based on the number of neurons in 1 3 mm area. The neuronal density in frontal lobe of cerebral cortex in Group I and Group II was recorded as 37074.73±605.76 and 36775.42±415.51 respectively. In temporal lobe, the neuronal density was 38896.97±720.07 and 38571.99±292.39 in Group I and Group II respectively. The density of neurons in occipital lobe of cerebral cortex was 40053.80±809.46 and 39842.70±575.80 in Group I and Group II respectively (Table 9, 10 and 11). These observations regarding neuronal density in cerebral cortex in almost all the lobes indicated that there was a linear decrease with the advancement of age. The neuronal density differed non significantly in all the lobes of cerebral cortex in Group I and Group II. Similar findings were reported by Fischer et al. (2012) regarding numerical neuronal density in the cerebral cortex showed a tendency of age-related decline. There was however, no significant correlation between decreasing neuronal number and increasing age in frontal, temporal and occipital lobes of cerebrum. In agreement with the present observations, Tigges et al. (1990) reported that the neuronal density decreases with the advancement of age in monkey; however they reported significant difference in neuronal density with the advancement of age, which was not significant in present study. Similar observations were also recorded by Beaulieu (1993) regarding numerical density of neurons in the occipital, the parietal and the frontal cortex of adult rat. Smiley et al. (2012) reported that there was no significant effect of schizophrenia on neuronal density in temporal lobe with advancement of age in human. In contrast to present study, Rockel et al. (1980) reported that there were 147,000 neurons present beneath 1 mm2 of cortical surface of area 4, which was much more in numbers than the findings of the present study. In contrast with the findings of present study Vincent et al. (1989) in rhesus monkeys and Terry et al. 42 (1987) in human reported that the neuronal density did not change significantly in any of the three cortical regions with advancement of age. Diao et al. (2009) reported no significant difference in the density of Nissl-stained neurons between young and old cats in primary visual cortex. Peinado et al. (1997) reported no age wise changes in neuronal density along with the increased number of glial cells. This was suggestive of aged-related cognitive impairment which had a consequence of neuronal dysfunction rather than actual neuronal losses. 4.2.1.2. The molecular layer: The uppermost layer of cerebral cortex showed the molecular layer. It was the outer most layer of cerebral cortex. It was thickest layer amongst all six layers. It contributed 22.40% of the total cortical depth. The thickness of this layer in frontal lobe of Group I varied from 381.99 µm to 430.42 µm with an average thickness of 405.05±18.66 µm. The thickness of this molecular layer in frontal lobe of Group II varied from 375.18 µm to 429.29 µm, with average thickness of 403.32±19.10 µm. The thickness of molecular layer in temporal lobe of Group I varied from 381.47 µm to 427.21 µm with average thickness of 400.42±14.44 µm. whereas in temporal lobe of Group II, it varied from 390.88 µm to 463.17 µm, with an average of 418.50±21.76 µm. Similarly, the thickness of molecular layer in occipital lobe of Group I was calculated as 383.22 µm to 436.34 µm with an average of 403.69±16.49 µm. While it ranged between 259.74 µm to 332.44 µm, in occipital lobe of Group II with an average of 300.46±22.89 µm (Table 3, 4 and 5). These observations indicated that there was linear decrease in the thickness of molecular layer in almost all the lobes under study with the advancement of age. Although, there was no significant decrease in the thickness of molecular layer of cerebral cortex in frontal lobe of Group I and Group II, but significant decrease was recorded in molecular layer of cerebral cortex between Group I and Group II in temporal as well as in occipital lobe with the advancement of age. Peters (2002) stated that the Layer 1 became thinner as apical tufts of pyramidal cells lose branches. That might be contributing to cognitive decline because it would cause a slowing of conduction along nerve fibers and disrupting the timing in neuronal circuits. The molecular layer of the cerebral cortex during present study showed that the number of small sized neurons in frontal lobe of Group I were in 43 the range of 22 to 59 with mean value 38.60 ±12.29. However, small sized neurons in Group II, varied from 24 to 59, with mean of 38.90±11.36. The small size neurons in temporal lobe of Group I varied between 28 to 58 with mean of 42.20±11.86, while in Group II, it varied from 29 to 55 with mean of 38.90±7.50. The small size neurons in occipital lobe of Group I ranged between 27 to 63 with the mean of 42.90±12.83. While, the small size neurons in Group II, varied between 34 to 68, with mean of 46.40±10.51. The medium size neurons in frontal lobe of Group I vary in-between 6 to 16, while the average number was 8.9±3.31. In Group II the medium size neurons vary in-between 3 to 13, while the average number was 8.0±2.82. The medium size neurons in temporal lobe of Group I vary in-between 06 to 14 with mean of 10.40±2.59 while in Group II it varies inbetween 07 to 14 with mean value of 10.40±2.31. The medium size neurons in occipital lobe of Group I ranged between 07 to 14 with mean of 10.20±2.40 while in Group II, it ranged between 06 to 14 with mean of 10±2.50 neurons (Table 6, 7 and 8). During the present study, it was noted that the small sized neuron as well as medium sized neuron in frontal, temporal, and occipital lobes of cerebral cortex did not show any significant difference between Group I and Group II with the advancement of age. It was noted that the large sized neurons were absent in almost all the lobes of cerebral cortex in Group I as well as in Group II. (Fig. 14 and 15). Available literature indicated that the molecular layer did not show any considerable large sized neuron in cerebral cortex in human beings. The molecular layer was found to be lodged with the small size spindle shaped neurons, which were scattered but their number was comparatively very less. The soma of the molecular neuron was stellate shaped and showed numerous cerebral vesicles. The Nissl substance was finely granular with centrally placed nuclei. Some neurons showed parallel orientation to surface and are called as „Cells of Cajal‟ (Fig. 16). The deeper layer of the molecular layer had some medium size scattered neurons. This peculiar arrangement might be attributed to focus on the maximum reception of stimuli from the surface and act as relay centres. In agreement with the findings of the present study, Zecevic and Rakic (2001) reported that remarkable perpendicular orientation of C-R cells processes in the plane parallel to the pial surface may be related to the cortical patterning and specification and a more precise geometrical pattern of laminar and columnar organization in the primate cortex. They further reported that only a subset of C-R cell survives in adulthood and many of them change their 44 morphology. They stated that the identification of several sub populations of layer I cell with selective persistence in primates may help in elucidating their possible role in congenital malformation of human cereberal cortex such as lissencephalic. The neuronal density in molecular layer of frontal lobe of cerebral cortex in Group I and Group II was recorded as 11769±1229 and 11565±790 respectively. In temporal lobe, the neuronal density was 13119±1006 and 11781±396 in Group I and Group II respectively. The density of neurons in occipital lobe of cerebral cortex was 13188±1100 and 18771±1149 in Group I and Group II respectively (Table 9, 10 and 11). These observations of the present study regarding neuronal density in molecular layer of frontal and temporal lobes of cerebral cortex in Group I and Group II showed linear decrease but there was no significant difference with the advancement of age. The neuronal density in molecular layer of occipital lobe of cerebral cortex showed significant linear increase. These observations are in agreement with those reported by Witelson et al. (1995) in human who recorded similar count with respect to neuronal density in molecular layer of temporal lobe of cerebral cortex. Greferath et al. (2000) in rat reported the similar number of neurons in layer I of cerebral cortex but reported much less neuronal density of small, medium and large size neurons in layer I. 4.2.1.3. The external granular layer: This layer was below the outer layer and had densely packed neurons and glial cells. This layer was found to contribute nearly 13.33% of the total cortical depth. The thickness of this layer in frontal lobe in Group I varied from 213.70 µm to 269.23 µm with an average thickness of 238.65±19.17 µm. The thickness of this layer in Group II showed variation from 203.97 µm to 249.23 µm, with an average thickness of 225.73±15.49 µm. The thickness of external granular layer in temporal lobe of Group I ranged from 247.18 µm to 213.43 µm with average thickness of 231.12±11.92 µm. The thickness of this layer in temporal lobe of Group II ranged from 276.33 µm to 223.13 µm, with the average of 248.60±21.01 µm. The thickness of external granular layer in occipital lobe of Group I ranged from 231.88 µm to 267.38 µm with average thickness of 251.24±11.59 µm. The thickness of this layer in occipital lobe of Group II ranged from 229.35 µm to 193.87 µm, with the average thickness of 212.97±12.30 µm 45 (Table 3, 4 and 5). Although no significant difference was noticed in thickness of external granular layer of cerebral cortex in frontal lobe, but significant difference was found in thickness of external granular layer of cerebral cortex in temporal and occipital lobes with advancement of age from Group I to Group II. The neurons in the external granular layer were stellate shaped with centrally located nuclei. These neurons were arranged perpendicular to surface. The cell size was found to increase towards the bottom of the layer (Fig. 17). Beaulieu (1993) observed ovoid and triangular neurons in second layer. He further stated that layer II showed marked increase in neuronal density. However, he did not mention any agewise change in the number of neurons. Walloe et al. (2014) reported that they observed granular cells in second layer of cerebrum in human. The number of small size neurons in frontal lobe of Group I and Group II were 120.70±18.40 and 129.30±13.20 respectively. The number of small size neurons in temporal lobe of Group I and Group II were 124±17.22 and 118.20±11.80 respectively. The number of small size neurons in occipital lobe of Group I and Group II 118.20±18.27 and 120.80±15.25 respectively. No significant difference was noticed in the number of small sized neurons in frontal, temporal and occipital lobes of granular layer of cerebral cortex with advancement of age. The number of medium size neurons in frontal, temporal and occipital lobes of external granular layer of Group I was 49.60±12.59, 47.50±9.36 and 42.10±8.86 respectively whereas in Group II these numbers were 44.0±11.20, 40.40±8.79. and 46.30±8.40 respectively. No significant difference was recorded during present study with respect to number of medium size neurons in all the regions of Group I and Group II with the advancement of age (Table 6, 7 and 8). Unlike first layer i.e. molecular layer of cerebral cortex, exhibited few, but scanty number of large neurons in external granular layer (Fig. 17). The number of large size neurons in frontal lobe of Group I ranged between 05 to 17 with a mean of 10.70±3.94, while in Group II it ranged between 08 to 23, with a mean of 13.80±4.84. The temporal lobe of Group I showed 07 to 16 number of large size neurons with a mean of 11.00±2.86, while in Group II it ranged from 08 to 16 with mean of 11.90±2.72. In occipital lobe of Group I, the number of large size 46 neurons ranged in-between 04 to 16 with mean of 10.40 ±3.43, while in Group II it ranged from 08 to 18 with mean of 12.20±3.36 neurons. The number of large size neurons in Group I and Group II did not show any significant difference with the advancement of age. The neuronal density in external granular layer of frontal lobe of cerebral cortex in Group I and Group II was recorded as 76102±3331 and 83175±2146 respectively. In temporal lobe, the neuronal density was 79140±2841 and 69098±3333 in Group I and Group II respectively. The density of neurons in occipital lobe of cerebral cortex was 68080±2738 and 84310±2609 in Group I and Group II respectively (Table 9, 10 and 11). These observations of the present study regarding neuronal density in frontal lobe of external granular layer of cerebral cortex did not show any significant difference between Group I and Group II with the advancement of age, however the neuronal density in temporal and occipital lobes showed significant difference. These observations recorded in present study regarding neuronal density in temporal and occipital lobes of granular layer of cerebral cortex are in agreement with those recorded by Walloe et al. (2014) in human. In contrast with the current findings, Gazzaley et al. (1997) reported that there was no significant difference in the total number of layer II neurons among juvenile, young adult, and aged monkeys. 4.2.1.4. The external pyramidal layer: This layer was nearly similar to the external granular layer in thickness. It contributed nearly 14% of the total cortical depth. The neurons had pyramidal shape. During the present study, it was noticed that the small pyramidal neurons were present towards the top of the layer, while the large pyramidal neurons were located at bottom part of the layer. These neurons were more densely packed. This layer was very easily demarcated from external granular and internal granular layer (Fig. 18). Similar observations were recorded by Games and Winer (1988) in albino rats measured thickness of third layer of cerebral cortex and stated that it constitutes about 16% of the total cortical depth. This observation is in concurrence with the present findings. In contrast with present observations, Cullen et al. (2006) in human noted that third layer constitutes about 31% of total cortical depth. Krimer et al. (1997) reported pyramidal shaped neurons in third layer of cerebral cortex in schizophrenic patients. In agreement with the 47 observations of the present study regarding shape of the neuronal cell body in third layer of cerebral cortex, Srivastava et al. (2009) described that the neuronal pyramidal cells were characterised by dendritic skirts immerging from triangular shape cell body. The thickness of external pyramidal layer in frontal lobe of Group I varied from 234.42 µm to 268.25 µm with average thickness of 249.93±12.24 µm. The thickness of this layer in Group II varied from 203.91 µm to 249.19 µm, with an average thickness of 221.70±15.91 µm. The thickness of external pyramidal layer in temporal lobe of Group I varied from 207.37 µm to 249.87 µm with an average thickness of 231.08±13.40 µm and in Group II, it varied from 167.43 µm to 199.21 µm, with an average thickness of layer was 184.47±11.47 µm. The thickness of external pyramidal layer in occipital lobe of Group I varied from 206.19 µm to 249.28 µm with average thickness of 228.91±16.28 µm, and in Group II it varied from 191.05 µm to 227.38 µm, with an average of 213.51±12.88 µm (Table 3, 4 and 5). A significant difference was noticed in the thickness of external pyramidal layer in frontal, temporal and occipital lobes of cerebral cortex in Group I and Group II with the advancement of age during the present study. Cullen et al. (2006) reported reduction in thickness of external pyramidal layer in schizophrenia. The external pyramidal layer showed more population of medium and large size neurons than small size neurons in comparison to other layers. The number of small size neurons in frontal lobe of Group I ranged between 43 to 86 with a mean of 67.40±12.66. In Group II, small size neurons varied from 39 to 67 with a mean of 54.60±8.79. The small size neurons in temporal lobe of Group I varied from 46 to 70 with mean of 58.90±7.70 while Group II, it varied from 40 to 69 with mean of 53.30±8.60. The small size neurons in occipital lobe of Group I, ranged between 51 to 78 with a mean of 65.20±9.00, while in Group II, it varied between 44 to 73, with a mean of 56.10±8.60. The population of small size neuron in frontal and occipital lobes showed significant difference between Group I and Group II. But no significant difference was noted in the population of small size neuron in temporal lobe. Rabinowicz et al. (2002) reported that average neuron size in the third layer of cerebral cortex was 185 sq. um. The size of this neuron is nearly similar to the small sized neuron of the present study. 48 The medium size neurons in frontal lobe of Group I varied from 41 to 71, with average number of 56.80±11.06. In Group II, the medium size neuron ranged in between 37 to 63 with an average of 47.30±9.04. The medium size neurons in temporal lobe of Group I varied from 39 to 60 with a mean of 48.50±7.90, while in Group II, it varied in between 32 to 63 with a mean value of 48.60±9.33. The medium size neurons in occipital lobe of Group I ranged between 38 to 65 with a mean of 50.90±9.37, while in Group II it ranged between 37 to 62 with mean of 49.70±9.32 neurons. The medium size neuron in all the three lobes of cerebrum did not show any significant difference with the advancement of age from Group I to Group II. The large size neurons in frontal lobe of Group I ranged in between 14 to 28 neurons with mean of 20.80±4.09, while in Group II, it ranged in between 15 to 24, with mean of 17.80±2.86. Similarly, in temporal lobe of Group I, it varied from 16 to 25 with a mean of 20.10±3.24, while in Group II, it was in the range of 13 to 23 with a mean of 18.10±3.63. In occipital lobe of Group I, large size neurons ranged in between 16 to 28 with a mean of 21.80±4.02, while in Group II, it varied from 11 to 24 with mean of 17.70±4.00 neurons. No significant difference in the number of large size neurons in frontal and temporal lobes of cerebrum was recorded with advancement of age, while occipital lobe showed significant difference. (Table 6, 7 and 8). The neuronal density in external pyramidal layer of frontal lobe of cerebral cortex in Group I and Group II was recorded as 55368±2031 and 56164±2808 respectively. In temporal lobe, the neuronal density was 55266±1850 and 65383±2842 in Group I and Group II respectively. The density of neurons in occipital lobe of external pyramidal layer in cerebral cortex was 60328±1555 and 57875±1645 in Group I and Group II respectively. These observations of the present study regarding neuronal density in frontal and temporal lobes of external pyramidal layer of cerebral cortex showed there was linear increase in the neuronal density from Group I to Group II. A significant difference was observed in temporal lobe between Group I and Group II with the advancement of age. In agreement with the findings of the present study, Meyer et al. (2010) in rat reported that the neuronal density in frontal lobe increases with the advancement of age, but there was no significant difference. However, in contrast with the findings of the present study, Cullen et al. (2006) reported that the overall 49 neuronal density in external pyramidal layer of cerebral cortex did not differ with advancement of age. They further stated that there was no correlation between neuronal density and age. In contrast with the findings of the present study, Yates et al. (2009) stated that although there was decrease in neuronal density in frontal and temporal lobes of cerebral cortex of rat, but there was no significant difference with the advancement of age 4.2.1.5. The internal granular layer: This layer is compressed in between external and internal pyramidal layer. It contributes nearly 19.32% of the total cortical depth. This layer consists of less number of large sized neurons compared to small and medium sized neurons. The cells found in this layer were surrounded by abundant glial tissue (Fig 19). In contrast with the findings of the present study, Semendeferi et al. (2001) reported that distribution of layer four makes up to 6% of total thickness of cerebral cortex in human, Chimpanzee and Bonobo, while in Gibbon it was up to 11%. Similarly, Games and Winer (1988) recorded thickness of layer four of cerebral cortex and stated that it constitutes about 10% of total thickness of cerebral cortex. During the present study, it was observed that the thickness of internal granular layer in frontal lobe of cerebral cortex in Group I varied from 321.76 µm to 365.23 µm with average thickness of 344.49±5.18 µm. The thickness of this layer in frontal lobe of Group II varied from 223.67 µm to 267.03 µm, with an average thickness of 244.50±4.70 µm. The thickness of internal granular layer in temporal lobe of Group I varied from 324.21 µm to 358.93 µm with an average thickness of 341.59±3.72 µm. The thickness of this layer in temporal lobe of Group II varied from 217.06 µm to 269.13 µm, with an average thickness of 241.55±5.36 µm. The thickness of internal granular layer in occipital lobe of Group I varied from 323.43 µm to 357.36 µm with an average of 338.50±3.95 µm. The thickness of this layer in occipital lobe of Group II varied from 208.63 µm to 253.17 µm, with an average of 231.20±5.10 µm (Table 3, 4 and 5). A significant difference was noted in the thickness of internal granular layer in frontal, temporal and occipital lobes of cerebral cortex in Group I and Group II with the advancement of age. There was linear decrease in the thickness of internal granular layer of cerebral cortex from Group I to Group II with the advancement 50 of age. Altamura et al. (2007) reported that the thickness of internal granular layer of cerebral cortex decreases with the advancement of age but they did not observed any significant difference with the advancement of age in mice. The small size neurons in frontal lobe of Group I ranged in-between 39 to 77 with the mean of 57.50±4.00. The small size neurons in Group II varied from 38 to 68 with the mean of 49.50±3.24. The small size neurons in temporal lobe of Group I varied in-between 43 to 69 with mean of 56.80±2.66, while in Group II, it varied from 48 to 72 with mean of 57.80±2.40. The small size neurons in occipital lobe of Group I ranged between 42 to 77, with the mean of 58.50±3.69. The small size neurons in Group II, varied from 43 to 70, with the mean of 56.20±2.48. There is no significant difference between the number of small size neuron in Group I and Group II. The medium size neurons in frontal lobe of Group I varied in-between 21 to 50, with the average number of 52.10±3.01. In Group II, the medium size neurons varied in-between 13 to 41, with an average number was 45.40±2.75. The medium size neurons in temporal lobe of Group I vary in-between 24 to 50 with mean of 36.50±2.68 while in Group II it varies in-between 19 to 44 with mean value of 29.40±2.30. The medium size neurons in occipital lobe of Group I ranged between 24 to 53 with mean of 38.30±3.18, while in Group II it were ranged between 25 to 47 with a mean of 36.00±2.57 neurons (Table 6, 7 and 8). These values indicated that the number of medium size neurons in temporal lobe of cerebral cortex showed significant difference with the advancement of age. However, the medium size neurons in temporal and occipital lobes of cerebral cortex did not show any significant difference between Group I and Group II. In this layer large size neurons in frontal lobe of Group I ranged inbetween 04 to 16 neurons with a mean of 8.30±1.2, while in Group II, it ranged in-between 06 to 13, with mean of 8.70±0.70. Similarly in temporal lobe of Group I, it varied from 07 to 14 with mean of 10.70±0.79, while in Group II, it varied from 07 to 14 with mean of 10.60±0.77. In occipital lobe of Group I, large size neurons ranged in-between 07 to 18 with mean of 11.60±1.31 while in Group II, it varied from 07 to 16 with mean of 11.50±1.65 neurons. No significant difference was noted during the present study in the large size neurons in frontal, temporal and 51 occipital lobes of cerebral cortex with the advancement of age from Group I to Group II. The neuronal density in internal granular layer of frontal lobe of cerebral cortex in Group I and Group II was recorded as 34199±1819 and 42331±2386 respectively. In temporal lobe, the neuronal density was 35210±1672 and 40664±1991 in Group I and Group II respectively. The density of neurons in occipital lobe of internal granular layer in cerebral cortex was 37366±1432 and 51792±2228 in Group I and Group II respectively. These observations of the present study regarding neuronal density in frontal and occipital lobes of internal granular layer of cerebral cortex showed significant difference between Group I and Group II with the advancement of age. In contrast with the findings of the present study, Beaulieu (1993) counted the Neuronal density in frontal, temporal and occipital lobes of cerebral cortex in adult rats, but their number was much less than that recorded during present study. Similarly, Witelson et al. (1995) in human recorded neuronal density in internal granular layer of cerebral cortex, which was also less than that recorded during present study. 4.2.1.6. The internal pyramidal layer: This layer was thick layer and contributed 18.87% of the total cortical thickness. In this layer, moderate quantities of large and medium size pyramidal cells were present surrounded by large quantity of glial tissue. This layer was densely packed due to larger size cells. This layer was found to contain more number of large size pyramidal cells compared to any other layer (Fig. 20). Some giant pyramidal neurons were also found in this layer called as Betz cell (Fig. 21). Similar observations were reported by Fischer et al. (2012) in human. They noted densely packed large pyramidal neurons in the internal pyramidal layer. Davanlou et al. (2004) reported similar findings and observed large conical cell bodies internal pyramidal layer of cerebral cortex in mouse. In agreement with the findings of the present study, Semendeferi et al. (2001) reported large darkly stained pyramidal cells in internal pyramidal layer of cerebral cortex in chimpanzee, gibbon and bonobo. Cullen et al. (2006) reported the thickness of internal pyramidal layer as 16%, which was reduced to 15% with the advancement of age in human. Games and Winer (1988) reported thickness of internal pyramidal layer as 26% of total thickness of cerebral cortex in albino rats. 52 The thickness of internal pyramidal layer in frontal lobe of cerebral cortex in Group I varied from 311.72 µm to 359.44 µm with an average of 337.66±5.9 µm. The thickness of this layer in frontal lobe of Group II varied from 305.78 µm to 349.15 µm, with an average thickness of 332.92±4.61 µm. The thickness of internal pyramidal layer in temporal lobe of Group I varied from 303.27 µm to 339.08 µm with an average of 322.32±3.91 µm. The thickness of this layer in temporal lobe of Group II varied from 318.32 µm to 377.81 µm, with an average thickness of 342.32±6.8 µm. The thickness of this layer in occipital lobe of Group I varied from 314.29 µm to 359.39 µm with an average thickness of 332.69±5.01 µm. The thickness of this layer in occipital lobe of Group II varied from 281.39 µm to 349.24 µm, with an average thickness of 314.21±8.10 µm. No significant difference was noted in the thickness of internal pyramidal layer of frontal and occipital lobes of cerebral cortex with the advancement of age from Group I to Group II during the present study. However, a significant difference was recorded in the thickness of internal pyramidal layer of temporal lobe in Group I and Group II. Games and Winer (1988) in albino rat reported that thickness of internal pyramidal layer was 270 µm which is less than the observations made in the present study. This variation in the observation could be attributed to the species difference and degree of motor activity of the portion of innervations concern. Beaulieu (1993) recorded much higher thickness of the internal pyramidal layer at frontal and occipital lobes of cerebral cortex in adult rat than that recorded during present study. The small size neurons in frontal lobe of Group I ranged in-between 41 to 73 with the mean of 60.40±3.34. The small size neurons in Group II varied from 39 to 61, while the mean numbers of neuron were 52.10±2.29. The small size neurons in temporal lobe of Group I ranged in between 49 to 74 with a mean of 61.00±2.65, while in Group II, it varied from 40 to 63 with a mean of 51.50±2.58. The small size neurons in occipital lobe of Group I ranged between 47 to 79 with a mean of 61.60±3.60. The small size neurons in Group II varied in-between 39 to 72 with the mean of 55.10±3.50. These figures recorded during present study indicated that there is linear decrease in the number of small size neurons in frontal, temporal and occipital lobes of cerebral cortex from Group I and Group II. But there was no significant difference between the numbers of small size neurons in frontal and occipital lobes of the cerebral cortex under study with the advancement of age. 53 The medium size neurons in frontal lobe of Group I varied from 29 to 52, with the average of 41.30±2.57. In Group II, the medium size neurons varied inbetween 33 to 50, while the average was 41.10±1.63. The medium size neurons in temporal lobe of Group I varied in between 41 to 63 with mean of 53.10±2.40 while in Group II, it varied in-between 31 to 59 with a mean value of 45.60±2.53. The medium size neurons in occipital lobe of Group I ranged between 36 to 63 with a mean of 51.90±3.11, while in Group II it ranged between 32 to 64 with a mean of 48.20±3.13 neurons (Table 6, 7 and 8). Although no significant difference was noticed in the number of medium size neurons in frontal and occipital lobes of cerebral cortex between Group I and Group II, but there was linear decrease in the number of medium size neurons in all the lobes of cerebral cortex with the advancement of age. In these layer large size neurons in frontal lobe of Group I ranged inbetween 14 to 29 neurons with a mean of 22.00±1.49, while in Group II, it ranged in-between 13 to 22, with mean of 17.70±0.98. Similarly, in temporal lobe of Group I, it ranged from 22 to 34 with a mean of 28.00±1.22, while in Group II, it was in the range of 21 to 31 with mean of 27.00±1.06. In occipital lobe of Group I, large size neurons ranged in-between 21 to 36 with mean of 28.90±1.41, while in Group II, it varied from 22 to 39 with a mean of 28.50±1.55 neurons. A significant difference was noticed in the number of large size neurons in frontal lobe, but temporal and occipital lobes of cerebral cortex did not show any significant difference in Group I and Group II with the advancement of age. Since no references are available in literature on number of small, medium and large sized neuron present in internal pyramidal layer of cerebral cortex, the values could not be compared. The neuronal density in internal pyramidal layer of frontal lobe of cerebral cortex in Group I and Group II was recorded as 36640±744 and 33401±915 respectively. In temporal lobe, the neuronal density was 44084±978 and 36295±896 in Group I and Group II respectively. The density of neurons in occipital lobe of internal pyramidal layer in cerebral cortex was 42694±791 and 39680±1289 in Group I and Group II respectively. These observations of the present study regarding neuronal density in frontal and temporal lobes of internal pyramidal layer of cerebral cortex showed significant difference between Group I and Group II with the advancement of age. However occipital lobe of cerebral 54 cortex did not show any significant difference between Group I and Group II with respect to neuronal density in internal pyramidal layer of cerebral cortex. 4.2.1.7. The multiform layer: This was the innermost layer of the cerebral cortex and somewhat less in thickness. This layer contributed nearly 12.88% of cortical depth. In this layer, mixture of pyramidal, stellate and large spindle shaped neurons was present which were surrounded by large numbers of glial cells. At the bottom of this layer, the spindle shaped neurons were present which demarcated this layer from deeper white matter zone (Fig. 22). Vincent et al. (1989) observed similar cells but described it as pyramidal and spiny stellate shaped cells in multiform layer of cerebral cortex in Macaque Monkey. However, in contrast with the findings of the present study, Games and Winer (1988) in albino rat observed closely packed flattened neuronal soma in multiform layer of cerebral cortex. They also reported that this layer constitutes about 22% of total cortical thickness. Cullen et al. (2006) however, reported that multiform layer constituted about 18% of total cortical thickness in human. During the present work, it was observed that the thickness of multiform layer in frontal lobe of Group I varied from 211.06 µm to 249.58 µm with an average thickness of 230.77±4.14 µm. The thickness of this layer in frontal lobe of Group II varied from 229.27 µm to 269.23 µm, with the average of 248.93±4.26 µm. The thickness of this layer in temporal lobe of Group I varied from 203.99 µm to 249.53 µm with an average of 227.43±5.68 µm. The thickness of multiform layer in temporal lobe of Group II varied from 312.19 µm to 279.13 µm, with an average of 240.20±6.67 µm. The thickness of this layer in occipital lobe of Group I varied from 214.48 µm to 259.31 µm with an average of 236.37±4.66 µm. The thickness of this multiform layer in occipital lobe of Group II varied from 273.43 µm to 328.46 µm, with an average of 298.05±6.62 µm. These observations of the present study showed that there is progressive increase in the thickness of multiform layer at frontal, temporal and occipital lobes of cerebral cortex with the advancement of age from Group I to Group II. There was a significant difference between increase in thickness of multiform layer at frontal and occipital lobes of cerebral cortex. But the temporal lobe of cerebral cortex did not show any significant difference between Group I and Group II with respect to increase in 55 thickness of multiform layer, although there was linear increase in thickness with the advancement of age. The number of small size neurons in frontal lobe of cerebral cortex in Group I ranged in between 23 to 44 with a mean of 34.70±2.21. The number of small size neurons in Group II varied from 22 to 49 with a mean of 38.80±2.62. The number of small size neurons in temporal lobe of Group I ranged in between 34 to 56 with a mean of 43.50±2.07, while in Group II, it varied from 31 to 53 with a mean of 40.40±2.08. The small size neurons in occipital lobe of Group I ranged in between 29 to 58, with a mean number of 40.50±2.96. The number of small size neurons in Group II ranged in between 27 to 52, with a mean of 38.70±2.19. The medium size neurons in frontal lobe of Group I varied in between 06 to 15, with an average of 10.20±0.97. In Group II, the number of medium size neuron varied in between 06 to 13, with an average of 9.2±0.68. The number of medium size neurons in temporal lobe of Group I varied in between 07 to 18 with a mean of 11.70±1.6, while in Group II, it varied in between 08 to 16 with mean value of 11.80±0.86. The medium size neurons in occipital lobe of Group I ranged between 07 to 21 with mean of 13.80±1.54, while in Group II it ranged between 08 to 20 with a mean of 13.80±1.18 neurons (Table 5, 6 and 7). In this layer large size neurons in frontal lobe of Group I ranged in between 0 to 4 neurons with a mean of 1.40±0.40, while in Group II, it ranged in between 0 to 4 with a mean of 1.60±0.45. Similarly in temporal lobe of Group I, it varied from 0 to 3 with a mean of 1.60±0.37, while in Group II, it varied from 0 to 3 with a mean of 1.60±0.26. In occipital lobe of Group I, large size neurons ranged in between 1 to 6 with a mean of 2.50±0.54, while in Group II, it varied from 0 to 4 with a mean of 1.60±0.40 neurons. These observations of the present study regarding number of small, medium and large size neurons in all the three lobes i.e. frontal, temporal and occipital lobes of cerebral cortex did not show any significant difference in Group I and Group II with the advancement of age. The neuronal density in multiform layer of frontal lobe of cerebral cortex in Group I and Group II was recorded as 20195±1456 and 17994±1247 respectively. In temporal lobe, the neuronal density was 24829±1352 and 56 22552±102 in Group I and Group II respectively. The density of neurons in occipital lobe of multiform layer in cerebral cortex was 24047±1344 and 18321±3808 in Group I and Group II respectively (Table 9, 10 and 11). These observations of the present study regarding neuronal density in multiform layer of occipital lobe of cerebral cortex showed significant difference between Group I and Group II with the advancement of age. While frontal and temporal lobe of cerebral cortex did not show any significant difference between Group I and Group II. In agreement with the findings of the present study, Yates et al.(2009) reported that although the neuronal density in multiform layer of cerebral cortex increases with the advancement of age in rats, but this increase was non significant. Witelson et al. (1995) recorded neuronal density in multiform layer of cerebral cortex in human which was much less than that recorded during the present study. This variation in the neuronal density could be attributed to the species difference. Cullen et al. (2006) in human reported that the neuronal density of cerebral cortex was reduced with the advancement of age. However, they stated that there was no correlation between neuronal density and age. 4.2.2. Cerebellum: The cerebellum was found to have a thin cortex of grey matter that overlies white matter. The cerebellum was found to have three layers from outside to inside as molecular layer, Purkinje cell layer and granular layer (Fig. 23, 24 and 25) 4.2.2.1. Molecular layer: The molecular layer was the outer most layer of cerebellar cortex, which consisted of neuropil, with medium to small sized scattered stellate and spindle shaped neurons. It also consisted of capillaries, that travels throughout molecular layer and become enlarged on entering the middle layer. This layer had a compact arborisation of dendrites originating from purkinje cells (Fig. 26). Molecular layer also contains axons of neurons of granular innermost layer of cerebellum which are believed to maintain continuity for propagation of signal throughout cerebellar tissue. Few basket cells were encountered at the deeper part of this layer (Fig. 27). Similar observations were recorded by Maseko et al. (2012) reported that stellate cells were present in upper 2/3rd and basket cells 57 were found in lower 1/3rd of cerebellar cortex in elephant, which is in agreement with the findings of the present study. During the present study, the thickness of molecular layer of cerebellar cortex in group I varied from 176.85 µm to 398.51 µm with an average thickness of 318.27±25.95 µm. The thickness of this layer in group II showed variation from 194.53 µm to 371.44 µm, with an average thickness of 303.72±17.46 µm. A gradual reduction in the thickness of molecular layer was noticed during present study from group I to group II. (Table 13) However, no significant difference was noticed in the thickness of molecular layer of cerebellar cortex in group I and group II with the advancement of age. The reduction in the thickness of molecular layer observed during the present study with the advancement of age could be attributed to loss of dendritic fibres of Purkinje cells entered in the molecular layer from the Purkinje cell layer as is being described by Zhang et al. (2006) who reported reductions in the thickness of molecular layer with the advancement of age in cat; however, in contrast with the findings of the present study, they reported that there was significant difference in the reduction of thickness of molecular layer with advancement of age in cat. In agreement with the findings of the present study, Pal et al. (2003) recorded the thickness of molecular layer in man and fowl as 343.5 µm and 294 µm respectively. Both these values recorded by them fall within the range of the present study. Similar observations regarding thickness of molecular layer of cerebellum were recorded by Fox and Barnard (1957) in adult monkey. The neuron number in molecular layer of cerebellar cortex in group I and group II was recorded as 68.9±2.48 and 63.4±2.65 respectively (Table 14). A non significant difference was noted in neuron number of molecular layer with advancement of age from group I to group II. Louis et al. (2014) reported neuron number as 25.6±4.53 in human, which is much less than that recorded during present study. This variation in the number of neuron may be attributed to the species difference. During the present work, the neuronal density in molecular layer of cerebellar cortex in group I and group II was recorded as 23204.84±2396 and 21920.35±2232 respectively (Table 15). A gradual decrease in the neuronal density was observed with the advancement of age. But there was no significant 58 difference in neuronal density of group I and group II. In agreement with the findings of the present study, Zhang et al. (2006) reported that the neuronal density in molecular layer of cerebellum decreases with the advancement of age in cat. However, they recorded significant decrease in neuronal density of molecular layer, which is in contrast with the current findings. 4.2.2.2. Purkinje cell layer : During the present study, it was observed that Purkinje cell layer was the second layer which was interposed in between the molecular layer and the granular layer of cerebellum. It was thinnest layer and consisted of chiefly Purkinje cells. These Purkinje cells were found scattered throughout this layer. These Purkinje cells were arranged in two ways, i.e. vertical and obliquely horizontal. At certain places, Purkinje cells were arranged in two rows, of which the upper cells were smaller in size than the lower cells (Fig. 27). Few Purkinje cells were flask shaped and of larger size with vertical orientation. These cells were found to dominate the middle layer. During the present work, it was noted that the thickness of Purkinje cell layer of cerebellar cortex in group I varied from 34.63 µm to 74.63 µm with an average thickness of 58.31±4.41 µm. In group II, the thickness of this layer was found to vary from 37.98 µm to 72.06 µm, with an average thickness of 55.66±4.14 µm. (Table 13) These values indicated that there was reduction in the thickness of Purkinje cell layer from group I to group II. But no significant difference was noticed in the decrease of thickness of Purkinje cell layer of cerebellar cortex with advancement of age from group I to group II. This decrease in thickness of Purkinje cell layer observed during the present study could be due to atrophic changes of dendritic arborisation of Purkinje cell during advancement of age. The number of neurons in Purkinje cell layer of cerebellar cortex in group I and group II were recorded as 10±0.55 and 9.8±0.41 respectively (Table 14). These values indicated that there was linear decrease in the number of neurons from group I to group II with the advancement of age. But there was no significant difference between the number of neurons in group I and group II. However, the neuronal density in Purkinje cell layer of cerebellar cortex in group I and group II was recorded as 18670.59±2325 and 18690.59±1770 respectively (Table 15), 59 which indicated that the neuronal density increased with the advancement of age from group I to group II. This increase is density of Purkinje cell might be the result of reduction in layer thickness. Woodruff-Pak et al. (2010) indicated significant loss of Purkinje neurons in aged mice. They reported that the processes of aging impact brain structures. The observations recorded by Viswasom et al. (2013) in human regarding decrease in number of Purkinje cells with advancement of age are in agreement with the findings recorded in the present study, however they reported significant difference in number of Purkinje cell with the advancement of age, which was not significant in present study. Nandy (1981) reported reduction in Purkinje cells significantly with age in Macaca nemestrina. Yesmin et al. (2011) reported that the number of Purkinje cell per square mm decreased with age which was significant in Bangaladesh people. They correlated this with changes in motor function in old age. In contrast to the observations recorded during present study, Whitney et al. (2008) reported non significant difference in reduction in density of Purkinje cells in control and acoustic brain in human. Similar findings regarding reduction in neuronal density of Purkinje cells with advancement of age was reported by Zhang et al. (2006) in cat. 4.2.2.3. Granular layer : The granular layer of cerebellum was the innermost layer. It lies below the Purkinje cell layer. This layer consisted of abundant neurons of small size granular cells. These cells were spherical in shape. The neurons were denser in upper layer, while towards bottom the cells become scantier. Few Golgi cells were found at the superficial part of this layer. The Golgi cells were larger than the granular cell. The Golgi cells were spindle shaped with centrally located nucleus (Fig. 29). These observations of the present study are in agreement with the findings reported by Maseko et al. (2012) in African Elephant. They observed Golgi cells within upper half of the granule cell layer. During the present work, it was observed that the thickness of granular layer of cerebellar cortex in group I was in the range of 311.85 µm to 549.58 µm with an average of 452.35±26.01 µm. The thickness of this layer in group II showed variation from 389.19 µm to 548.27 µm, with an average thickness of 471.46±19.63 µm (Table 13). Although there was linear increase in the thickness 60 of granular layer from group I to group II, but no significant difference was noticed in the thickness of granular layer of cerebellar cortex with advancement of age. These findings of the present study regarding increase in thickness of granular layer corroborates with the observations reported by Zhang et al. (2006) in cat. However, in contrast with the findings of the present study, they reported significant increase in thickness of granular layer with advancement of age. The measurements regarding thickness of granular layer reported by Pal et al. (2003) in fowl and man were less than that recorded during present study and do not corroborate with observations of the present study. This variation could be attributed to the species variation. The number of neuron in Granular cell layer of cerebellar cortex in group I and group II was recorded as 288.40±9.01 and 286.90±5.71 respectively (Table 14). There was no significant difference between group I and group II regarding number of neurons in granular layer with advancement of age. However, the values recorded showed that there was progressive decrease in the number of neuron from group I to group II. This observation of the present study is in agreement with the findings recorded by Viswasom et al. (2013) in human. They also noted progressive decrease in number of granular cells with the advancement of age. Renovell et al. (1996) reported significant decline in number of granular cells with advancement of age in human. Although similar decline was noted in the number of granular cell during present study, but there was no significant difference in group I and group II with advancement of age. The neuronal density in Granular cell layer of cerebellar cortex in group I and group II was recorded as 65379.71±3716 and 61750.72±2714 respectively (Table 15). This observation of the present study showed that there was progressive decrease in the density of granular cells from group I to group II with the advancement of age. However, no significant difference was noted in group I and group II. In accordance with the findings of the present study, Zhang et al. (2006) reported that density decreased by 27% with advancement of age in cat, but in contrast with the present findings they reported that there was significant difference in the neuronal density with advancement of age. Renovell et al. (1996) reported that the total number of Granular cells decline significantly during the aging process in human. Nandy (1981) found no age related changes in granular density in Macaca –nemestrina. 61 4.2.3. Pons During the present study, it was observed that the pons contain large number of nerve fibres with few neurons. The fibre tracks were noted to be well organized in the form of nerve fascicles. In cross section, the nerve fibres were surrounded by distinct epineurium, which send its septa and divide it into small fascicles by perinurium and each axon was covered by endoneurium and myelin sheath. Oligodendrocyte cells with distinct nucleoli were adjacent to axon (Fig. 30). In between the fibres, large number of glial cells were found which were of round to stellate shaped with centrally located nuclei. Very few large multipolar neurons were also found at deep layer. With appreciable perineuronal spaces especially around the large neurons were observed. During the present work, three types of neuron were identified in the Pons based on their size. These neurons were categorised according to the perimeter of the soma as small (1 to 100 µm), medium (101 to 200 µm) and large (more than 200 µm) (Fig 31). In group I, the number of small neurons was recorded as 20±0.93; whereas in group II, these numbers were recorded as 17.8±0.813. The number of medium size neurons of group I were recorded as 9.1±0.525, whereas in group II, these numbers were recorded as 9±0.614. Similarly, the large sized neuron were 3.1±0.433 in pons of group I while in group II, the number of neurons were 2.6±0.33 (Table 16). From the values regarding number of small, medium and large neurons recorded during present study, it was observed that the number of neurons decreased from group I to group II with the advancement of age. However, this decrease in the number of neurons from group I to group II was statistically non significant. The overall number of neurons in pons irrespective of size also did not show any significant difference between group I (32.2 ±1.152) and group II (29.4±0.90), although the number was found reduced with the advancement of age (Table 16). The neuronal density of neurons in Pons was recorded as 3220±115.27 in group I, while in group II it was recorded as 2940±90.921. This figure indicated that there was reduction in density with the advancement of age from group I to group II (Table 16). The neuronal density of neuron in pons did not show any significant difference between group I and group II. This reduction in the number 62 as well as density of neuron in pons may be related to the loss of neuron with the age factor. These observations of the present study could not be compared with the observations of the other scientists since very little literature is available regarding this parameter. 4.2.4. Medulla oblongata During the present study, the medulla oblongata in all the age groups was noted to be made up of chiefly the broad bands of nerve fibres, which were longitudinally placed and had some oligodendrocyte cells scattered amongst them (Fig. 32). In between these fibres, neurons of spindle and stellate type were observed. Although all size of neurons were found in the superficial layer, but only medium to large sized neuronal cells were observed in deeper layer of medulla oblongata (Fig. 33). In the present study, the number of small neurons was 14±0.73 in group I, whereas in group II, these numbers were noted as 13.4±1.00. The number of medium size neurons in group I and group II were recorded as 6±0.39 and 4.9±0.37 respectively. Similarly, the large sized neuron were 1.8±0.29 in medulla oblongata of group I, while in group II, the numbers were 1.7±0.21 (Table 17). From the values regarding number of small, medium and large neurons recorded during present study, it was observed that the number of neurons decreased from group I to group II with the advancement of age. However, this decrease in the number of neurons from group I to group II was statistically non significant. The overall number of neurons in medulla oblongata irrespective of size was 21.8±0.69 and 20±1.14 in group I and group II respectively (Table 17). These values also did not show any significant difference between group I and group II, although the number was found reduced with the advancement of age. The neuronal density of neurons in medulla oblongata was recorded as 2180±69.60 in group I, while in group II it was recorded as 2000±114.50 (Table 17). These figures indicated that there was reduction in density with the advancement of age from group I to group II. The neuronal density of neuron in medulla oblongata did not show any significant difference between group I and group II. 63 This reduction in the number as well as density of neuron in medulla oblongata may be related to the loss of neuron with the age factor. These observations of the present study could not be compared with the observations of the other scientists since very little literature is available regarding this parameter. From the observations of the present study regarding reduction in number of neurons as well as reduction in density of neuron from group I to group II with advancement of age, it can be concluded that there is permanent loss of neuron with the advancement of age. 4.3. Histochemistry and histoenzymology 4.3.1. Glycogen: 4.3.1.1. Cerebrum: In the present study, the PAS activity for presence of glycogen in small neurons of almost all the layers of cerebrum at frontal, temporal and occipital lobes in group I showed weak activity, but the blood vessels and neuropil showed mild PAS positive activity (Fig.34, 35 and 36) while in group II with the advancement of age, the PAS activity was recorded as mild to moderate in small neurons of almost all layers of cerebrum, blood vessels and neuropil of frontal, temporal and occipital lobes (Fig. 37, 38 and 39). The large neurons in various layers of cerebrum at frontal, temporal and occipital lobes however showed mild PAS positive activity in group I. The activity for presence of glycogen in large neurons of group II exhibited mild to moderate activity (Fig. 40 and 41). These observations regarding presence of glycogen in small neurons, large neurons, blood vessels and neuropil of cerebrum indicated that the PAS activity increases with the advancement of age from group I to group II. These observations of present study regarding increasing trend in PAS activity with the advancement of age are in agreement with the findings reported by Manich et al. (2016) who reported progressive increase in PAS activity in brain of aged mice. In agreement with findings of present study Capucchio et al. (2010) reported that the PAS positive activity increases with age in horse brain. The presence of glycogen is related with the energy requirement for metabolism. The continuous decrease in number of neurons with the advancement of age observed during present study might lead to additional 64 metabolic burden on the available neurons, blood vessels and neuropil and may require more energy to carry out its normal function. 4.3.1.2. Cerebellum : During the present study it was noted that there was mild to moderate PAS positive activity for the presence of glycogen in neuropil, molecular cells, purkinje cells and granular cells of cerebellum in group I (Fig. 42). With the advancement of age in group II the PAS activity for the presence of glycogen was observed as moderate to intense in neuropil, molecular cells, purkinje cells and granular cells of cerebellum (Fig. 43). Observations of the present study corroborates with the findings reported by Gerhauser et al. (2012) in hamster, who reported PAS positive activity in few large neurons in cerebellum but they did not report any agewise difference. The increase PAS positive activity for presence of glycogen in cerebellum with advancement of age observed during present study might be ascribed to the more energy requirement for carrying out cerebellar function during advanced age. 4.3.1.3. Pons : The neuronal cells of the pons showed weak PAS positive activity in group I where as in group II mild PAS positive activity was noted in the neuronal cells of pons with the advancement of age (Fig. 44 and 45). This increase in PAS positive activity could be attributed to the deposition of lipofuscin granules during advancement of age. 4.3.1.4. Medulla oblongata: During the present study weak PAS positive activity was observed in cellular component of medulla oblongata in group I, where as weak to mild PAS positive activity was noticed in the medulla oblongata in group II with the advancement of age (Fig. 46 and Fig. 47). Similar observations were reported by Gerhauser et al. (2012) in hamster. They reported PAS positive activity in few large neurons of medulla oblongata in oldest group. The presence of PAS activity during advanced age recorded during present study could be due to deposition of lipofuscin granules. 65 4.3.2. Acid phosphatase: 4.3.2.1. Cerebrum: During the present work, it was observed that the acid phosphatase activity in small as well as large neurons, blood vessels and neuropil of different layers at frontal, temporal and occipital lobes of cerebrum was mild in group I, while in group II, this activity was moderate in all the components of cerebrum in all the lobes with the advancement of age (Fig. 48 and 49). Similar findings were reported by Sohal and Sharma (1972) in aged flies brain. They recorded accumulation of dense residual bodies of acid phosphatase. However, in contrast with the findings of the present study, Nakamura et al. (1989) found no significant age dependent changes in rat. The findings of the present study regarding increasing trend of acid phosphatase activity with the advancement of age may be indicative of continuous alterations occurring in cerebrum due to degeneration of neurons. Although, the exact physiological function of acid phosphatase in cerebrum is not known, the fundamental significance of phosphatase was their ability to catalyse the hydrolases of phosphate esters. 4.3.2.2. Cerebellum: The acid phosphatase activity in neurons, neuropils and blood vessels of cerebellum in group I exhibited mild activity, while in group II, the acid phosphatase activity was moderate (Fig. 50 and 51). There was gradual increase in the activity of acid phosphatase with the advancement of age from group I to group II. Similar findings were also recorded by Manocha (1970) in aged squirrel monkey. They relate this activity to static maintenance metabolism of cells than to dynamic functional metabolism. The report of occurrence of acid phosphatase activity with the advancement of age in present study was contrary to the statements made by Nakamura et al. (1989) in rat. They stated that although acid phosphatase activity was noted in cerebellum, but there was no significant age dependent change in the activity of acid phosphatase. Release of lysosomal hydrolases associated with modifications in neuronal cells occurring during ageing might be the probable reason for increase in the acid phosphatase activity with the advancement of age observed during present study. 66 4.3.2.3. Pons: In the present study, the acid phosphatase activity in all the cellular elements of pons was moderate in group I, whereas it was intense in group II with the advancement of age (Fig. 52 and 53). In agreement with the observations of the present study, Mohankumar and Sood (1979) reported that all the cellular elements in pons of Hedgehog were positive for acid phosphatase activity with the advancement of age. Similarly, Nakamura et al. (1989) reported significant age dependent changes in pons of rat. The increased acid phosphatase activity with the advancement of age observed during present study could be due to accumulation of acid phosphatase positive dense residual bodies over the pons during advanced age. 4.3.2.4. Medulla oblongata: The acid phosphatase activity in all the cellular elements of medulla oblongata during present work was found to be moderate in group I, whereas it was intense in group II with the advancement of age (Fig. 54 and 55). This observation of the present study is in agreement with that reported by Mohankumar and Sood (1979) in medulla oblongata of Hedgehog. They reported positive activity for acid phosphatase in all the components of medulla oblongata with the advancement of age. Similar observations were also reported by Hafiza and Sood (1979) in bat. They noted strong activity in the large neurons of medulla oblongata. The findings of the present study regarding increasing trend of acid phosphatase activity with the advancement of age from group I to group II in all the components of medulla oblongata may be attributed to the alterations caused due to degeneration of neurons in medulla oblongata or accumulation of dense residual bodies or may be ascribed to the phagocytosis which is likely to occur after degeneration of neuronal cells. However, the exact cause of intense acid phosphatase activity remained unknown. 67 4.3.3. Alkaline phosphatase : 4.3.3.1. Cerebrum : During the present work, it was observed that the alkaline phosphatases activity in small as well as large neurons, blood vessels and neuropils of different layers at frontal, temporal and occipital lobe of cerebrum was weak in group I where as in group II it was mild (Fig. 56 and 57). There was increase in the activity of alkaline phosphatase with the advancement of age. Manocha (1970) reported that the alkaline phosphatase activity was concentrated in the blood vessels and the peripheral part of the neurons of cerebrum. In agreement with the findings of the present study, Kellett et al. (2011) reported increasing alkaline phosphatase activity on neuronal membrane in human brain injury and Alzheimer‟s disease. Vardy et al. (2012) in human reported that there was no increase in alkaline phosphatase activity with the advancement of age, but the activity was found increased in brain of Alzheimer‟s disease. 4.3.3.2. Cerebellum: The alkaline phosphatase activity in neurons, neuropils and blood vessels of cerebellum in group I as well as group II exhibited weak to mild activity (Fig. 58 and 59). In agreement with the findings of the present study, Ng and Tam (1986) stated that alkaline phosphates activity increases with advancement of age in mouse. 4.3.3.3. Pons: In the present study, the alkaline phosphatase activity in all the cellular elements of pons was mild and moderate in group I and group II respectively (Fig. 60 and 61). In agreement with the observations of the present study, Mohankumar and Sood (1979) reported that all the cellular elements in pons of Hedgehog were positive for alkaline phosphatase activity with the advancement of age. 68 4.3.3.4. Medulla oblongata: The alkaline phosphatase activity in all the cellular elements of medulla oblongata during present work was found to be moderate in group I as well as in group II (Fig. 62 and 63). There was no difference in the intensity of activity with the advancement of age. In contrast with the findings of the present study, Mohankumar and Sood (1979) reported that all the cellular elements in medulla oblongata of Hedgehog were positive for alkaline phosphatase activity during advanced age. 4.3.4. Lipofuscin: 4.3.4.1. Cerebrum : During the present work, mild lipofuscin pigmentation was observed in small neurons and large neuron of different layers at frontal, temporal and occipital lobes of cerebrum in group I where as in group II, moderate to intense lipofuscin pigmentation was noted with the advancement of age (Fig. 64 and 65). The lipofuscin pigmentation in general was found more towards the axon hillock and the periphery of the neuron in small as well as large neurons of group I and group II. It was observed that the number of cells containing pigment increased with age. It was also noted that due to heavy lipofuscin pigmentation, the nucleus of the large neuron was shifted toward periphery with the advancement of age (Fig. 66). Similar observations were reported by Whiteford (1964). In agreement with the findings of the present study, Tigges (1990) stated that the lipofuscin granules were discernible in large neurons in cerebrum of monkey. He further mentioned that these lipofuscin granules increased with the advancement of age. Findings of the present study were also supported by Nesic et al. (2013), who reported that there was agewise progression in deposition of lipofuscin in large neurons of cerebrum in human. Whiteford (1964) stated that although agewise increase in deposition of lipofuscin in neuron was observed in dog and pig, but the deposition was independent of breed and sex. These findings corroborates with the observations of the present study. Gilissen et al. (1999) observed accumulation of lipofuscin in cerebrum of dwarf lemur and gray lemur only in aged animals but not in young animals. These findings are also in accordance with the observations of the present study. 69 The formation or deposition of lipofuschin, although is not clearly understood, but it is felt that agewise increase in number of lipodal granules in neurons with advancement of age observed during present study may due to the fact that the homogenous distribution of the granules might be getting gradually lost and may gathered in clusters in the cell body to form finally a localised mass, which might continue to increase in size with the advancement of age. Similarly, the accumulation of lipofuscin deposition even in young age cannot be eliminated either by degradation or by exocytosis and get accumulated in cell and since oxidative reactions are obligatory for life, they act as age dependent enhancer of lipofuscin accumulation with the advancement of age. 4.3.4.2. Cerebellum: During the present work, weak to mild pigmentation was observed in Purkinje cells, molecular cells and granule cells of cerebellum in group I, whereas in group II, moderate lipofuscin pigmentation was recorded in Purkinje cells, molecular cells and granule cells of cerebellum. This indicated that the pigmentation increased with the advancement of age from group I to group II (Fig. 67 and 68). Similar findings were reported by Heinsen (1981) and Monteiro (1991) in rats. They recorded increased lipofuscin pigment in the perikarya of Purkinje cells. Gilissen et al. (1999) investigated lipofuscin deposits in large Purkinje cells of dwarf lemur with advancement of age. Findings of the present study regarding increased lipofuscin pigmentation with advancement of age also corroborates with the findings reported by Nesic et al. (2013) and Gilissen et al. (1999) who reported age related progressive lipofuscin accumulation in cerebellum of aged cheirogaleids lemur. Nandy (1981) reported deposition of lipofuscin in Purkinje cells in relation to aging in Macaca nemestrina. These observations of the present study as well as findings of other scientists related with progressive accumulation of lipofuscin in cerebellum with the advancement of age are a basic biological ageing process. The lipofuscin pigments, which were present in the region of dendritic attachment, are likely to cause interference with the propagation of nerve impulse. It can also be concluded that the lipofuscin pigment may be detrimental to the cells by virtue of being a rigid mass leading to disturbance in plasticity of the cells. 70 4.3.4.3. Pons : Pons in general showed weak to mild lipofuscin pigmentation in neurons of group I, while in group II, there was moderate pigmentation of lipofuschin. This indicated that the pigmentation increased with the advancement of age from group I to group II (Fig. 69 and 70). In agreement with the findings of the present study, Gilissen et al. (1999) reported that accumulation of lipofuscin was observed only in aged cheirogaleids lemur but not in young ones. The findings of the present study are also supported by Whiteford (1964) in dog and pig. He stated that the amount of pigment contained within the individual cells and the number of cells containing pigment increased with age, but were independent of breed and sex. 4.3.4.4. Medulla oblongata: During the present study, a weak to mild lipofuscin pigmentation in neurons of medulla oblongata was observed in group I, while in group II, moderate pigmentation of lipofuscin was recorded. There was linear increase in lipofuscin pigmentation in medulla oblongata with the advancement of age from group I to group II (Fig. 71 and 72). These findings of the present study are in accordance with those reported by Whiteford (1964) in dog and pig. He reported that the amount of pigmentation of lipofuscin within the individual cells increased with age. Nesic et al. (2013) observed progressive agewise increase in accumulation of lipofuscin in neurons of medulla oblongata. This observation is also in agreement with the findings of the present study. The findings of the Gilissen et al. (1999) regarding lipofuscin accumulation in medulla oblongata of aged lemur also corroborates with the findings of the present study. The age wise increasing trend of lipofuscin accumulation of neuron of cerebrum, cerebellum, pons and medulla oblongata observed during present study could be justified by considering different modalities operating within the nerve cells such as Lipofuscin is deposited in cell as a result of metabolic process. Initial pigment deposition occurs in relation to level of activity characteristic of a nuclear group. Within a cell group lipofuscin accumulates as a function of time. 71 Accumulation of lipofuscin in neuron may be a criterion of basic biological aging processes. 4.4. Electron microscopy 4.4.1. Cerebrum : During the present study, cerebrum of young goats, at 10 months of age showed mild fatty deposits scattered in almost all the organelles of cell. These fatty deposits were electron dense material and appeared as black coloured spots (Fig. 73 and 74). With the advancement of age there was progressive granulation followed by mild pigmentation on myelin sheath (Fig. 75). The myelin sheath was found to undergo splitting and cytoplasm of oligodendrocyte appeared as electron dense, which subsequently appeared as projected myelin balloons (Fig. 76). The myelin balloon was found to increase in size and caused disruption of topography of neuronal cells. Due to increase in size of myelin balloons, the vacuoles are formed and nucleus gets pushed towards one side (Fig. 77). These observations of the present study are in agreement with the findings reported by Peters (2002) in human and non human primates. He stated that some myelin sheath exhibit degenerative changes such as splitting of myelin sheath and formation of balloons with the advancement of age. He further suggested that such degenerative changes lead to cognitive decline since they cause change in conduction velocity, which results in dysfunction of normal timing in neuronal circuits. He further mentioned that due to degeneration, other changes such as formation of redundant myelin and increase thickness of myelin, which were observed, could be suggestive of continuation of some myelin formation with advancement of age. Similar observations were also recorded by Peters et al. (2000) and Vincent et al. (1989), they reported that in old monkey the vacuoles appeared to represent a late stage in the degeneration of myelinated axons, for that they were bounded by a thin, laminated sheath. 4.4.2. Cerebellum: The electron microscopy of cerebellum in group I, showed electron dense material in the cytoplasm of the neuron, which was considered as fatty deposits (Fig. 78). The purkinje cells of cerebellum showed presence of electron dense material of fatty bodies (Fig. 79). The deposits of fat, which appeared as electron 72 dense material, were found in the myelin sheath of the young goats (Fig. 80). During the present study, it was observed that the neurons of the cerebellum undergo degenerative changes with the advancement of age. Initially, the degenerative process was found to start with shrinkage of mitochondria (Fig. 81) and regression of axon making the cell non functional. Further the process of apoptosis continued and nucleus became narrow and fat deposits appeared in nucleoplasm (Fig. 82). Finally the neurons of the cerebellum undergo complete degeneration with total shrinkage of mitochondria and cell was considered as apoptic (Fig. 83). 4.4.3 Pons: In young goats of group I, the pons showed less fatty deposits with no granulation as well as no degenerative changes in the organelles of neuronal cells (Fig. 84). However, with the advancement of age in group II, almost all the organelles of neuronal cells of pons exhibited disruption. The mitochondria appeared hedgy (Fig. 85). Similarly, myelin showed large vesicular appearance with very little cytoplasm (Fig. 86). 4.4.4. Medulla oblongata: The electron microscopic structure of medulla oblongata in young goats of group I showed normal structure of all the cell organelle. There were no fatty deposits in neuropil of medulla oblongata (Fig. 87). However, little granulation was present in myelin sheath of medulla oblongata of young goats (Fig. 88). In group II, with the advancement of age, myelin sheath of the medulla oblongata showed electron dense material of fatty deposits. The degenerated mitochondria in the form of vesicles showed electron lucent areas and were found gathered at one place (Fig. 89). 73 5. SUMMARY AND CONCLUSIONS The present work was carried on 20 samples each of cerebrum, cerebellum, pons and medulla oblongata of goat divided into two groups as 9 to 12 months and 13 months and above. The biometrical observation of whole brain, were recorded and samples of cerebrum, cerebellum, pons and medulla oblongata were cut and processed for histological, histochemical, histoenzymic and electron microscopy. The tissue sections were stained with hematoxylin and eosin, Toluidine blue, Gallocyanin, Luxol fast and silver impregnation stains for histological observations. The tissues were also stained to verify the presence of glycogen, acid phosphatase, alkaline phosphatase and lipofuscin. Agewise changes in myelin degeneration, pigment deposition and mitochondrial changes, if any, were studied by Electron microscopic method. The gross morphology indicated that the cerebrum of goat was oblong to oval in shape. The cerebellum was oval with wider dimension along transverse axis. Pons was transversely elongated structure located on ventral aspect of brain stem with faint median furrow. The medulla oblongata was distal continuation of brain and continued as spinal cord. A highly significant difference was observed in the weight of whole brain in Group I and Group II with the advancement of age. There was linear decrease in the length, width and depth of brain with advancement of age. However, no significant difference in the length, width and the depth of brain was observed. There was no significant difference between the length and width of cerebrum with the advancement of age, but the length as well as width of cerebrum was found increased with the advancement of age. Although, the depth of cerebrum showed slight decrease from Group I to Group II, but there was no significant difference in the depth of cerebrum with the advancement of age. No significant difference between the length and width of cerebellum was observed with advancement of age. There was no significant difference in the brain index of Group I and Group II. Similarly, the cerebral index also did not show any statistical difference 74 between Group I and Group II. There was no significant difference between Group I and II with respect to cerebellar index. The microstructure of cerebral cortex in almost all the regions of the cerebrum viz. frontal, temporal and occipital lobes in Group I as well as in Group II, showed six layers with scattered neurons supported by varying amount of glial tissue viz. the molecular layer, the external granular layer, the external pyramidal, the internal granular, the internal pyramidal and the multiform layer. The cortical depth at all the three lobes of cerebrum under study decreased with the advancement of age. There was significant difference in the cortical depth of the frontal, temporal and occipital lobes of cerebrum in Group I and Group II. Three types of neuron were identified in the cerebral cortex based on the size as small, medium and large. Different shapes of neurons were recorded during the present study viz. spindle, pyramidal and stellate. The total number of neuron in frontal and temporal lobes of cerebral cortex showed highly significant difference between Group I and Group II with the advancement of age. However, occipital lobe of cerebral cortex did not show any significant difference between Group I and Group II with respect to total number of neuron in cerebral cortex. These observations regarding neuronal density in cerebral cortex in almost all the lobes indicated that there was linear decrease with the advancement of age. The neuronal density differed non significantly in all the lobes of cerebral cortex in Group I and Group II. The uppermost layer of cerebral cortex showed the molecular layer. Although, there was no significant decrease in the thickness of molecular layer of cerebral cortex in frontal lobe of Group I and Group II, but significant decrease was recorded in thickness of molecular layer of cerebral cortex between Group I and Group II in temporal as well as in occipital lobe with the advancement of age. The small sized neuron as well as medium sized neuron in frontal, temporal, and occipital lobes of cerebral cortex did not show any significant 75 difference between Group I and Group II with the advancement of age. It was noted that the large sized neurons were absent in almost all the lobes of cerebral cortex in Group I as well as in Group II. The soma of the molecular neuron was stellate shaped and showed numerous cerebral vesicles. Some neurons showed parallel orientation to surface and are called as „Cells of Cajal‟. The neuronal density in molecular layer of frontal and temporal lobes of cerebral cortex in Group I and Group II showed linear decrease but there was no significant difference with the advancement of age. The neuronal density in molecular layer of occipital lobe of cerebral cortex showed significant linear increase from Group I and Group II. No significant difference was noticed in thickness of external granular layer of cerebral cortex in frontal lobe, but significant difference was found in thickness of external granular layer of cerebral cortex in temporal and occipital lobes with advancement of age from Group I to Group II. The neurons in the external granular layer were stellate shaped with centrally located nuclei. These neurons were arranged perpendicular to surface. No significant difference was noticed in the number of small sized neurons in frontal, temporal and occipital lobes of granular layer of cerebral cortex with advancement of age. No significant difference was recorded during present study with respect to number of medium size neurons in all the regions of Group I and Group II with the advancement of age. The numbers of large size neurons in Group I and Group II did not show any significant difference with the advancement of age. The neuronal density in frontal lobe of external granular layer of cerebral cortex did not show any significant difference between Group I and Group II with the advancement of age, however, the neuronal density in temporal and occipital lobe showed significant difference. A significant difference was noticed in the thickness of external pyramidal layer in frontal, temporal and occipital lobes of cerebral cortex in Group I and 76 Group II with the advancement of age. The external pyramidal layer showed more population of medium and large size neurons than small size neurons in comparison to other layers. The population of small size neuron in frontal and occipital lobe showed significant difference between Group I and Group II. But no significant difference was noted in the population of small size neuron in temporal lobe. The number of medium size neuron in all the three lobes of cerebrum did not show any significant difference with the advancement of age from Group I to Group II. No significant difference in the number of large size neurons in frontal and temporal lobes of cerebrum was recorded with advancement of age, while occipital lobe showed significant difference. A significant difference was observed in neuronal density of temporal lobe between Group I and Group II with the advancement of age. A significant difference was noted in the thickness of internal granular layer in frontal, temporal and occipital lobes of cerebral cortex in Group I and Group II with the advancement of age. The neuronal density in frontal and occipital lobes of internal granular layer of cerebral cortex showed significant difference between Group I and Group II with the advancement of age. The internal pyramidal layer was densely packed due to larger size cells. This layer was found to contain more number of large size pyramidal cells compared to any other layer. No significant difference was noted in the thickness of internal pyramidal layer of frontal and occipital lobes of cerebral cortex with the advancement of age from Group I to Group II during the present study. However, a significant difference was recorded in the thickness of internal pyramidal layer of temporal lobe in Group I and Group II. There was no significant difference between the number of small size neurons in frontal and occipital lobes of the internal pyramidal layer of the cerebral cortex with the advancement of age. No significant difference was noticed in the number of medium size neurons in frontal and occipital lobes of cerebral cortex between Group I and Group II, but there was linear decrease in the number of medium size neurons in all the lobes of cerebral cortex with the advancement of age. A significant difference was noticed in the number of large 77 size neurons in frontal lobe but temporal and occipital lobes of cerebral cortex did not show any significant difference in Group I and Group II with the advancement of age. Neuronal density in frontal and temporal lobes of internal pyramidal layer of cerebral cortex showed significant difference between Group I and Group II with the advancement of age. Occipital lobe of cerebral cortex did not show any significant difference between Group I and Group II with respect to neuronal density in internal pyramidal layer of cerebral cortex. A significant difference was noticed between Group I and Group II with respect to increase in thickness of multiform layer at frontal and occipital lobe of cerebral cortex. But the temporal lobe of cerebral cortex did not show any significant difference between Group I and Group II with respect to increase in thickness of multiform layer. The number of small, medium and large size neurons in all the three lobes i.e. frontal, temporal and occipital lobe of cerebral cortex did not show any significant difference in Group I and Group II with the advancement of age. The neuronal density in multiform layer of occipital lobe of cerebral cortex showed significant difference between Group I and Group II with the advancement of age. While, frontal and temporal lobe of cerebral cortex did not show any significant difference between Group I and Group II. No significant difference was noticed in the thickness of molecular layer of cerebellar cortex in group I and group II with the advancement of age. There was no significant difference in neuronal density of group I and group II. No significant difference was noticed in the decrease of thickness of Purkinje cell layer of cerebellar cortex with advancement of age from group I to group II. The neuronal density increased with the advancement of age from group I to group II. No significant difference was noticed in the thickness of granular layer of cerebellar cortex with advancement of age. There was progressive decrease in the density of granular cells from group I to group II with the advancement of age. However, no significant difference was noted in group I and group II. 78 It was observed that the pons contain large number of nerve fibres with few neurons. The number of small, medium and large neurons in pons decreased from group I to group II with the advancement of age. The decrease in the number of neurons from group I to group II was statistically non significant. The overall number of neurons in pons irrespective of size also did not show any significant difference between group I and group II. The neuronal density of neuron in pons did not show any significant difference between group I and group II. The medulla oblongata at all the age groups was noted to be made up of chiefly the broad bands of nerve fibres, which were longitudinally placed. The numbers of small, medium and large neurons were decreased from group I to group II with the advancement of age. However, this decrease in the number of neurons from group I to group II was statistically non significant. The neuronal density of neuron in medulla oblongata did not show any significant difference between group I and group II. Regarding reduction in number of neurons as well as reduction in density of neuron from group I to group II with advancement of age, it can be concluded that there is permanent loss of neuron with the advancement of age. The presence of glycogen in small neurons, large neurons, blood vessels and neuropil of cerebrum increases with the advancement of age from group I to group II. It was noted that there was mild to moderate PAS positive activity for the presence of glycogen in neuropil, molecular cells, purkinje cells and granular cells of cerebellum in group I. With the advancement of age in group II the PAS activity for the presence of glycogen was observed as moderate to intense in neuropil, molecular cells, purkinje cells and granular cells of cerebellum. The neuronal cells of the pons showed weak PAS positive activity in group I, where as, in group II mild PAS positive activity was noted. The PAS positive activity was not observed in any cellular component of medulla oblongata in group I, where as weak to mild activity was noticed in group II with the advancement of age. The acid phosphatase activity in small as well as large neurons, blood vessels and neuropil of different layers at frontal, temporal and occipital lobes of cerebrum was mild in group I, while in group II, it was moderate. The acid phosphatase activity in neurons, neuropils and blood vessels of cerebellum in 79 group I exhibited mild activity, while in group II, it was moderate. The acid phosphatase activity in all the cellular elements of pons was moderate in group I, whereas it was intense in group II. The acid phosphatase activity in all the cellular elements of medulla oblongata was found to be moderate in group I, whereas it was intense in group II with the advancement of age. The alkaline phosphatases activity in small as well as large neurons, blood vessels and neuropils of different layers at frontal, temporal and occipital lobe of cerebrum was weak in group I, where as in group II it was mild. The alkaline phosphatase activity in neurons, neuropils and blood vessels of cerebellum in group I as well as group II exhibited weak to mild activity. The alkaline phosphatase activity in all the cellular elements of pons was mild and moderate in group I and group II respectively. The alkaline phosphatase activity in all the cellular elements of medulla oblongata during present work was found to be moderate in group I as well as in group II. Mild lipofuscin pigmentation was observed in small and large neuron of different layers at frontal, temporal and occipital lobes of cerebrum in group I whereas in group II, moderate to intense lipofuscin pigmentation was noted with the advancement of age. It was also noted that due to heavy lipofuscin pigmentation, the nucleus of the large neuron was shifted toward periphery with the advancement of age. Weak to mild pigmentation was observed in Purkinje cells, molecular cells and granule cell of cerebellum in group I, where as in group II, moderate lipofuscin pigmentation was recorded. Pons in general showed weak to mild lipofuscin pigmentation in neurons of group I, while in group II, there was moderate pigmentation of lipofuschin. A weak to mild lipofuscin pigmentation in neurons of medulla oblongata was observed in group I, while in group II, moderate pigmentation was recorded. Cerebrum of young goats, at 10 months of age showed mild fatty deposits scattered in almost all the organelles of cell. Electron microscopically cerebrum showed progressive granulation followed by mild pigmentation on myelin sheath. The myelin sheath was found to undergo splitting and cytoplasm of oligodendrocyte appeared as electron dense, which subsequently appeared as projected myelin balloons. Due to increase in size of myelin balloons, the vacuoles are formed and nucleus gets pushed towards one side. The neurons of 80 the cerebellum were found to undergo complete degeneration with total shrinkage of mitochondria and cell was considered as apoptic with the advancement of age. Electron microscopically almost all the organelles of the neuronal cells of pons exhibited disruption. The mitochondria appeared hedgy and myelin showed large vesicular appearance with very little cytoplasm with the advancement of age. Electron microscopically the degenerated mitochondria in the form of vesicles showed electron lucent areas and were found gathered at one place during advancement of age in medulla oblongata. 81 Fig. 1: Photomicrograph showing cerebral cortex with piamater (P) and blood vessel (B) (Gr. I). (Haematoxylin and Eosin X 100) Fig. 2: Photomicrograph showing cerebral cortex with six layers viz. molecular, outer granular, outer pyramidal, inner granular, inner pyramidal and multiform layer (Gr.- I, Frontal lobe) (Gallocyanine X 50) Fig. 3: Photomicrograph showing cerebral cortex with six layers viz. molecular, outer granular, outer pyramidal, inner granular, inner pyramidal and multiform layer (Gr.- II, Frontal lobe) (Gallocyanine X 50) Fig. 4: Photomicrograph showing cerebral cortex with six layers viz. molecular, outer granular, outer pyramidal, inner granular, inner pyramidal and multiform layer (Gr.I,Temporal lobe) (Gallocyanine X 50) Fig. 5: Photomicrograph showing cerebral cortex with six layers viz. molecular, outer granular, outer pyramidal, inner granular, inner pyramidal and multiform layer (Gr.- II, Temporal lobe) (Gallocyanine X 50) Fig. 6: Photomicrograph showing cerebral cortex with six layers viz. molecular, outer granular, outer pyramidal, inner granular, inner pyramidal and multiform layer (Occipital lobe, Gr. I) (Haematoxylin & Eosin X 50) Fig. 7: Photomicrograph showing different layers of cerebral cortex viz. molecular, outer granular, outer pyramidal, inner granular, inner pyramidal and multiform layer (Gr.II, Occipital lobe) (Gallocyanine X 100) Fig. 8: Photomicrograph showing small neuron (S) and area calculated by Q-imaging software. (Gr.-I cerebral cortex) (Toluidine blue X 1000) Fig. 9: Photomicrograph showing medium neuron (M) and area calculated by Q-imaging software. (Gr.-II cerebral cortex) (Toluidine blue X 1000) Fig. 10: Photomicrograph showing Large neuron (L) and area calculated by Q-imaging software. (Gr.-I cerebral cortex) (Toluidine blue X 1000) Fig. 11: Photomicrograph showing spindle shape neuron (N). (Gr.- I cerebral cortex) (Toluidine blue X 1000) Fig.12: Photomicrograph showing pyramidal shape neuron (N) (Gr.- II cerebral cortex) (Toluidine blue X 1000) Fig. 13: Photomicrograph showing stellate shape neuron (N) (Gr. -II cerebral cortex) (Toluidine blue X 1000) Fig. 14: Photomicrograph showing molecular layer of cerebral cortex. (Gr.- I, Occipital lobe) (Gallocyanine X 100) Fig. 15: Photomicrograph showing molecular layer of cerebral cortex. (Gr.- II, Frontal lobe) (Toluidine blue X 200) Fig. 16: Photomicrograph showing cells of Cajal (C) in layer I. (Gr. -I, cerebral cortex) (Haematoxylin & Eosin X 200) Fig. 17: Photomicrograph showing external granular layer of cerebral cortex. (Gr.- I, Frontal lobe) (Toluidine blue X 200) Fig. 18: Photomicrograph showing external pyramidal layer of cerebral cortex. (Gr.- II, temporal lobe) (Toluidine blue X 200) Fig. 19 Photomicrograph showing internal granular layer (arrow) of cerebral cortex. (Gr.- I, Frontal lobe) (Gallocyanin X 200) Fig. 20: Photomicrograph showing internal pyramidal layer of cerebral cortex. (Gr.- II, temporal lobe) (Toluidine blue X 200) Fig. 21: Photomicrograph showing internal pyramidal layer and Betz cells (arrow) of cerebral cortex. (Gr.- I, frontal lobe) (Toluidine blue X 200) Fig. 22 : Photomicrograph showing multiform layer of cerebral cortex and white matter (arrow). (Gr.- II, temporal lobe) (Toluidine blue X 200) Fig. 23: Photomicrograph showing molecular layer (M), Purkinje cell layer (P) and Granule cell layer (G). (Gr.-I, cerebellar cortex) (Haematoxylin & Eosin X 200) Fig. 24: Photomicrograph showing molecular layer (M), Purkinje cell layer (P) and Granule cell layer (G). (Gr.-II, cerebellar cortex) (Haematoxylin & Eosin X 100) Fig. 25: Photomicrograph showing molecular layer (M), Purkinje cell layer (P) and Granule cell layer (G) (Gr.-II, cerebellar cortex) (Silver impregnation X 100) Fig. 26: Photomicrograph showing arborisation (arrow) from Purkinje cell. (Gr.-II, cerebellar cortex) (Luxol fast X 200) Fig. 27 : Photomicrograph showing Basket cells (arrow). (Gr.-II, cerebellar cortex) (Toluidine blue X 400) Fig. 28: Photomicrograph showing Purkinje cells (arrow). (Gr.-I, cerebellar cortex) (Haematoxylin & Eosin X 200) Fig. 29: Photomicrograph showing Golgi cells (arrow). (Gr.- I, cerebellar cortex) (Haematoxylin & Eosin X 200) Fig. 30: Photomicrograph showing Epineurium (E), Perinurium (P) and Oligodendrocytes (arrow). (Gr.-II, Pons) (Toluidine blue X 200) Fig. 31: Photomicrograph showing large (L), medium (M) and small (S) neurons (Gr.-I, Pons) (Toluidine blue X 100) Fig. 32: Photomicrograph showing nerve fibres and oligodendrocyte (arrow) (Gr.-II, medulla oblongata) (Toluidine blue X 200) Fig. 33: Photomicrograph showing large cells (Gr.- II, medulla oblongata ) Haematoxylin & Eosin X 200) Fig. 34: Photomicrograph of cerebrum showing weak PAS activity in small neuron (N) and mild activity in blood vessel (B) and neuropil (P). (frontal lobe, Gr. I) (PAS reaction x 200) Fig. 35: Photomicrograph of cerebrum showing weak PAS activity in small neuron (N) and mild activity in blood vessel (B) and neuropil (P). (temporal lobe, Gr. I) (PAS reaction x 200) Fig. 36: Photomicrograph of cerebrum showing weak PAS activity in small neuron (N) and mild activity in blood vessel (B) and neuropil (P). (occipital lobe, Gr. I) (PAS reaction x 200) Fig. 37: Photomicrograph of cerebrum showing mild to moderate PAS activity in small neuron (N), blood vessel (B) and neuropil (P). (frontal lobe, Gr. II) (PAS reaction x 200) Fig. 38: Photomicrograph of cerebrum showing mild to moderate PAS activity in small neuron (N), blood vessel (B) and neuropil (P). (temporal lobe, Gr. II) (PAS reaction x 200) Fig. 39: Photomicrograph of cerebrum showing mild to moderate PAS activity in small neuron (N), blood vessel (B) and neuropil (P). (occipital lobe Gr. II) (PAS reaction x 200) Fig. 40: Photomicrograph of cerebrum showing mild PAS activity in large neuron (L). (Gr. I) (PAS reaction x 400) Fig. 41: Photomicrograph of cerebrum showing mild to moderate PAS activity in large neuron (L). (Gr. II) (PAS reaction x 200) Fig. 42: Photomicrograph of cerebellum showing mild to moderate PAS activity in molecular cell (M), Purkinje cell (P), Granular cell and Neuropil (PL). (Gr.I) (PAS reaction x 100) Fig. 43: Photomicrograph of cerebellum showing moderate to intense PAS activity in molecular cell (M), Purkinje cell (P), Granular cell and Neuropil (PL). (Gr. II) (PAS reaction x 200) Fig. 44: Photomicrograph of pons showing weak PAS activity in large neuron (L). (Gr. I) (PAS reaction x 100) Fig. 45 : Photomicrograph of pons showing mild PAS activity in large neuron (L). (Gr. II) (PAS reaction x 200) Fig. 46: Photomicrograph of medulla oblongata showing weak PAS positive activity in neuronal cell. (Gr. I) (PAS reaction x 100) Fig.47: Photomicrograph of medulla oblongata showing weak to mild PAS positive activity. (Gr. II) (PAS reaction x 200) Fig. 48: Photomicrograph of cerebrum showing mild acid phosphatase activity in blood vessel (B), neuron (N) and neuropil (P). (Gr. I) (Acid phosphatase x 200) Fig. 49: Photomicrograph of cerebrum showing mild acid phosphatase activity in blood vessel (B), neuron (N) and neuropil (P). (Gr. II) (Acid phosphatase x 200) Fig. 50: Photomicrograph of cerebellum showing moderate acid phosphatase activity in molecular cell (M), Purkinje cell (P), Granular cell and Neuropil (PL). (Gr. I) (Acid phosphatase x 200) Fig. 51: Photomicrograph of cerebellum showing moderate acid phosphatase activity in molecular cell (M), Purkinje cell (P), Granular cell (G) and Neuropil (PL). (Gr. II) (Acid phosphatase x 100) Fig. 52: Photomicrograph of pons showing moderate acid phosphatase activity in cellular elements. (Gr. I) (Acid phosphatase x 100) Fig. 53: Photomicrograph of pons showing intense acid phosphatase activity. (Gr. II) (Acid phosphatase x 100) Fig. 54: Photomicrograph of medulla oblongata showing moderate acid phosphatase activity in large neuron (arrow). (Gr. I) (acid phosphatase x 100) Fig. 55: Photomicrograph of medulla oblongata showing moderate acid phosphatase activity in large neuron. (Gr. II) (Acid phosphatase x 100) Fig. 56: Photomicrograph of cerebrum showing weak alkaline phosphatase activity in neurons (arrow). (Gr. I) (Alkaline phosphatase x 200) Fig. 57 : Photomicrograph of cerebrum showing mild alkaline phosphatase activity. (Gr. II) (Alkaline phosphatase x 200) Fig. 58 : Photomicrograph of cerebellum showing weak to mild alkaline phosphatase activity. (Gr. I) (Alkaline phosphatase x 200) Fig. 59 : Photomicrograph of cerebrum showing weak to mild alkaline phosphatase activity. (Gr. II) Fig. 60: (Alkaline phosphatase x 200) Photomicrograph of pons showing mild alkaline phosphatase activity. (Gr. I) (Alkaline phosphatase x 100) Fig. 61: Photomicrograph of pons showing moderate alkaline phosphatase activity. (Gr. II) (Alkaline phosphatase x 100) Fig. 62 : Photomicrograph of medulla oblongata showing moderate alkaline phosphatase activity. (Gr. I) (Alkaline phosphatase x 200) Fig. 63: Photomicrograph of medulla oblongata showing moderate alkaline phosphatase activity. (Gr. II) (Alkaline phosphatase x 200) Fig. 64: Photomicrograph of cerebrum showing mild lipofuscin deposits in large and small neuron (arrow). (Gr. I) (Sudan black B x 200) Fig. 65: Photomicrograph of cerebrum showing moderate to intense lipofuscin deposits in neurons (arrow). (Gr. II) (Sudan black B x 200) Fig. 66: Photomicrograph of cerebrum showing moderate to intense lipofuscin deposits (arrow) and displaced nucleus (arrow yellow). (Gr. II) (Sudan black B x 1000) Fig. 67: Photomicrograph of cerebellum showing weak to mild lipofuscin deposits. (Gr. I) (Sudan black B x 100) Fig. 68: Photomicrograph of cerebellum showing moderate lipofuscin deposits in Purkinje cell (arrow). (Gr. II) (Sudan black B x 1000) Fig. 69: Photomicrograph of pons showing weak to mild lipofuscin deposits. (Gr. I) (Sudan black B x 400) Fig. 70 : Photomicrograph of pons showing moderate lipofuscin deposits. (Gr. II) (Sudan black B x 1000) Fig. 71: Photomicrograph of medulla oblongata showing weak to mild lipofuscin deposits. (Gr. I) (Sudan black B x 200) Fig. 72 : Photomicrograph of medulla oblongata showing moderate lipofuscin deposits. (Gr. II) (Sudan black B x 200) Fig. 73: Transmission electron photomicrograph showing normal neuron from cerebrum with fatty deposit (arrow). (Gr. I) (X 9.6 M) Fig. 74: Transmission electron photomicrograph showing normal neuron with electron dense material (arrow). (cerebrum Gr. I) (X 5.7 M) Fig. 75: Transmission electron photomicrograph showing granulation (G), pigmentation (P) and myelin degeneration (M) (cerebrum Gr. II) (X13.5 M) Fig. 76: Transmission electron photomicrograph showing nerve fiber (M) bulging (B) and dense cytoplasm (C). (cerebrum Gr. II) (X 12 M) Fig. 77: Transmission electron photomicrograph showing nerve cell and displaced nucleus (arrow). (cerebrum, Gr. II) (X 7.7 M) Fig. 78: Transmission electron photomicrograph showing nerve cell and fat deposites (arrow). (cerebellum, Gr. I) (X 4.8 M) Fig. 79: Transmission electron photomicrograph showing nerve cell and fat deposits (arrow). (cerebellum, Gr. I) (X 4.8 M) Fig. 80: Transmission electron photomicrograph showing myelin sheath (cerebellum, Gr. I) and deposits (arrow) (X 57.9 M) Fig. 81: Transmission electron photomicrograph showing degenerating neuron and shrunken mitochondria (arrow). (cerebellum, Gr. II) (X 9.6 M) Fig. 82: Transmission electron photomicrograph showing degenerating neuron and shrunken mitochondria (arrow) with dense rim. (cerebellum, Gr. II) (X 7.7 M) Fig. 83: Transmission electron photomicrograph showing apoptic neuron, shrunken mitochondria (M) and regressed axon (arrow). (cerebellum, Gr. II) (X 6.7 M) Fig. 84: Transmission electron photomicrograph showing normal neuron and very less deposits (arrow). (Pons, Gr. I) (X 7.7 M) Fig. 85: Transmission electron photomicrograph showing degenerative neuron and cloudy mitochondria (arrow). (Pons, Gr. II) (X 7.7 M) Fig. 86: Transmission electron photomicrograph showing vesicular appearance of myelin (M) and scanty cytoplasm (C). (Pons, Gr. II) (X 77.2 M) Fig. 87: Transmission electron photomicrograph showing normal neuron. (medulla oblongata, Gr. I). (X 7.7 M) Fig. 88: Transmission electron photomicrograph showing normal myelin sheath (medulla oblongata, Gr. I) and deposits (arrow). (X 23 M) Fig. 89: Transmission electron photomicrograph showing myelin sheath mitochondria (M) and deposits (arrow). (medulla oblongata, Gr. II) (X 15.5 M) Table 1 : Biometrical observations of brain in group I and group II Group I Observations Group II Sample mean SD SE Sample mean SD SE T –stat F-cal P Weight of brain (gm) 120.30 8.87 2.85 131.60 5.50 1.75 3.41 2.87* 0.003 Length of brain (cm) 9.91 0.50 0.15 9.77 0.43 0.13 0.66 2.10 0.51 Width of brain (cm) 6.33 0.50 0.16 6.23 0.32 0.10 0.52 2.13 0.60 Depth of brain (cm) 3.98 0.19 0.06 3.96 0.30 0.09 0.17 2.13 0.86 Length of cerebrum (cm) 7.82 0.50 0.15 8.13 0.34 0.11 1.60 2.11 0.12 Width of cerebrum (cm) 6.04 0.57 0.18 6.26 0.44 0.13 0.96 2.10 0.34 Depth of cerebrum (cm) 3.98 0.19 0.06 3.96 0.30 0.09 0.17 2.13 0.86 Length of cerebellum (cm) Width of cerebellum (cm) Depth of cerebellum (cm) * significant at 1% 3.12 0.31 0.10 3.15 0.31 0.10 0.21 2.10 0.83 4.70 0.69 0.21 4.42 0.48 0.15 1.12 2.11 0.27 3.01 0.16 0.05 3.11 0.21 0.06 1.18 2.10 0.25 Table 2: Showing different indices of brain in group I and group II Group I Group II Observations Sample mean SD SE Sample mean SD SE T –stat F-cal P Brain Index 157.21 11.57 3.65 157.38 13.64 4.31 0.03 2.10 0.97 Cerebral Index 130.54 14.8 4.68 130.4 10.52 3.32 0.02 2.11 0.98 Cerebellar Index 151.8 21.49 6.79 140.79 13.51 4.27 1.37 2.13 0.190 Table 3: Micrometrical observations showing thickness of different layers of frontal lobe of cerebral cortex in group I and group II. (µm) Group I Layers Group II SD SE Total Depth Sample mean 1785.77 SD SE T –stat F-cal P 12.75 Sample mean 1666.82 40.32 57.36 18.13 5.31 2.92* 6.94 Layer I 405.05 18.66 5.90 403.32 19.10 6.04 0.20 2.10 0.83 Layer II 238.65 19.17 6.60 225.73 15.49 4.9 1.65 2.10 0.11 Layer III 249.93 12.24 3.87 221.70 15.91 5.03 4.43 2.89* 0.00 Layer IV 344.49 16.38 5.18 244.50 15.14 4.78 15.16 2.87* 1.06 Layer V 337.66 18.77 5.90 332.92 14.61 4.62 0.63 2.10 0.53 Layer VI 230.77 13.10 4.14 248.93 13.48 4.26 3.05 2.87* 0.00 * significant at 1% Table 4 : Micrometrical observations showing thickness of different layers of temporal lobe of cerebral cortex in group I and group II. Group I Layers Group II SD SE Total Depth Sample mean 1745.5 SD SE T –stat F-cal P 11.57 Sample mean 1669.10 36.61 51.29 16.2 3.832 2.920** 0.0015 Layer I 400.42 14.44 4.56 418.50 21.76 6.88 2.188 2.119* 0.043 Layer II 11.92 13.40 3.77 4.23 248.60 21.01 6.64 2.288 2.144* 0.03 Layer III 231.12 231.08 184.47 11.47 3.63 8.351 2.87** 1.32 Layer IV 341.59 11.79 3.72 241.55 16.97 5.36 15.304 2.920** 5.65 Layer V 322.32 12.39 3.91 342.32 21.53 6.8 2.54 2.144* 0.023 Layer VI 227.43 17.98 5.68 240.20 21.09 6.67 0.909 2.100 0.374 **significant at 1% , *significant at 5% level Table 5 : Micrometrical observations of thickness of different layers of occipital lobe of cerebral cortex in group I and group II. Group I Layers Sample mean SD Group II SE Total Depth 1781.8 32.66 10.33 Sample mean 1565.5 Layer I 403.69 16.49 5.21 Layer II 251.24 11.59 3.66 Layer III 228.91 16.28 Layer IV 338.5 Layer V Layer VI SD SE T –stat F-cal P 73.87 23.36 8.47 3.05** 2.09 300.46 22.89 7.23 11.56 2.92** 3.48 12.3 3.89 7.159 2.878** 1.14 5.15 212.97 213.51 12.88 2.34 2.10* 0.03 12.51 3.95 231.2 16.15 4.07 5.1 16.63 2.10** 5.90 332.69 15.86 5.01 314.21 25.64 1.93 2.13 0.07 236.37 14.75 4.66 298.05 20.96 7.60 2.92** 1.05 ** significant at 1% , * significant at 5% level 8.1 6.62 Table 6 : Micrometrical observations showing neuronal count of different layers of frontal lobe of cerebral cortex of group I and group II. * significant at 5% level / ** significant at 1% level Layer I Group I Group II Statistic Layer II Layer III Layer IV Layer V Layer VI Size S M L S M L S M L S M L S M L S M L Mean 38.6 8.9 - 120.7 49.60 10.7 67.40 56.80 20.80 57.50 52.1 8.30 60.40 41.30 22.0 34.70 10.20 1.4 SD 12.2 3.31 - 18.40 12.59 3.94 12.66 11.06 4.09 12.65 9.53 3.91 10.58 8.15 4.73 6.99 3.08 1.26 SE 3.88 1.04 - 5.82 3.98 1.24 4.00 3.49 1.65 4.00 3.01 1.20 3.34 2.57 1.49 2.21 0.97 0.4 Mean 38.9 8.0 - 129.3 44 13.8 54.60 47.30 17.80 49.50 45.4 8.70 52.10 41.10 17.70 33.80 9.2 1.6 SD 11.3 2.82 - 13.20 11.20 4.84 8.79 9.04 2.86 10.24 8.69 2.21 7.24 5.17 3.129 8.31 2.15 1.43 SE 3.59 0.89 - 4.17 3.54 1.53 2.78 2.86 0.90 3.24 2.75 0.70 2.29 1.63 0.98 2.62 0.68 0.45 T –stat 0.05 0.65 1.20 2.10 2.10 2.11 2.10 1.54 1.55 1.24 0.28 2.04 0.06 2.39 0.26 0.84 0.33 F-cal 2.10 2.10 - 2.11 2.10 2.10 2.62* 2.10 2.10 2.10 2.11 2.14 2.11 2.13 2.11* 2.10 2.11 2.10 P value 0.95 0.52 - 0.24 0.30 0.13 0.01 0.05 0.13 0.13 0.23 0.78 0.05 0.94 0.02 0.79 0.41 0.74 Table 7: Micrometrical observations showing neuronal count of different layers of temporal lobe of cerebral cortex in group I and group II. Layer I Group I Group II Statistic Layer II Layer III Layer IV Layer V Layer VI Size S M L S M L S M L S M L S M L S M L Mean 42.2 10.40 - 124 47.50 11.0 58.9 48.5 20.1 56.8 36.50 10.7 61.00 53.10 28.0 43.5 11.70 1.60 SD 11.86 2.59 - 17.22 9.36 2.86 7.7 7.90 3.24 8.41 8.47 2.49 8.38 7.59 3.88 6.57 3.36 1.17 SE 3.75 0.81 - 5.44 2.96 0.96 2.45 2.56 1.02 2.66 2.68 0.79 2.65 2.40 1..22 2.07 1.065 0.371 Mean 38.90 10.40 - 118.2 40.40 11.9 53.3 48.6 18.1 57.8 29.40 10.6 51.50 45.60 27.0 40.4 11.80 1.6 SD 7.5 2.31 - 11.8 8.79 2.72 8.6 9.33 3.63 8.27 7.94 2.45 8.16 8.03 3.36 6.60 2.74 0.83 SE 2.3 0.73 - 3.75 2.78 0.86 2.74 2.9 1.14 2.40 2.30 0.77 2.58 2.53 1.06 2.08 0.86 0.26 T –stat 0.74 0.0 - 0.87 0.41 0.71 1.52 0.02 1.29 0.26 5.45 0.09 2.56 2.14 0.67 1.05 0.07 0.00 F-cal 2.13 2.10 - 2.11 2.11 2.10 2.10 2.10 2.10 2.10 2.10** 2.10 2.10* 2.10* 2.10 2.10 2.10 2.11 P value 0.46 1.0 - 0.39 0.68 0.48 0.14 0.97 0.21 0.79 4.30 0.92 0.01 0.04 0.50 0.30 0.94 1.00 * significant at 5% level / ** significant at 1% level Table 8: Micrometrical observations showing neuronal count of different layers of occipital lobe of cerebral cortex of group I and group I. * significant at 5% level / ** significant at 1% level Layer I Group I Group II Statistic Layer II Layer III Layer IV Layer V Layer VI Size S M L S M L S M L S M L S M L S M L Mean 42.90 10.20 - 118.2 42.10 10.40 65.20 50.90 21.80 58.50 38.30 11.60 61.60 51.90 28.90 40.50 13.80 2.50 SD 12.83 2.40 - 18.27 8.68 3.43 9.00 9.37 4.02 11.69 10.06 4.14 11.39 9.86 4.48 9.36 4.89 1.71 SE 4.05 0.77 - 5.70 2.74 1.08 2.84 2.96 1.27 3.69 3.18 1.31 3.60 3.11 1.41 2.96 1.54 0.54 Mean 46.40 10 - 120.80 46.30 12.20 56.10 49.70 17.70 56.20 36.0 11.5 55.10 48.20 28.50 38.70 13.80 1.60 SD 10.51 2.5 - 15.25 8.40 3.36 8.60 9.32 4.00 7.80 8.13 3.13 11.26 9.89 4.97 6.92 3.73 1.26 SE 3.3 0.81 - 4.80 2.60 1.06 2.70 2.94 1.26 2..48 2.57 1.65 3.5 3.13 1.55 2.19 1.18 0.40 T –stat 0.66 0.17 - 0.34 1.09 1.18 2.30 0.28 2.28 0.51 0.86 0.06 1.28 0.83 0.18 0.48 0.0 1.33 F-cal 2.10 2.10 - 2.10 2.10 2.10 2.10* 2.10 2.10* 2.11 2.10 2.10 2.10 2.10 2.10 2.10 2.10 2.10 P value 0.51 0.86 - 0.73 0.28 0.25 0.03 0.77 0.03 0.61 0.39 0.95 0.21 0.41 0.85 0.63 1.00 0.19 Table 9: Neuron density of different layers of frontal lobe of cerebral cortex in group I and group II. Group I Group II Layers Sample mean SD SE SD SE T –stat F-cal P 605 Sample mean 36775 Total Depth 37075 1915 1314 415 0.34 2.11 0.68 Layer I 11769 3889 1229 11565 2498 790 0.140 2.131 0.89 Layer II 76102 10536 3331 83175 6789 2146 1.784 2.131 0.09 Layer III 55368 6424 2031 56164 8880 2808 0.22 2.11 0.82 Layer IV 34199 5752 1819 42331 7546 2386 2.71 2.10* 0.01 Layer V 36640 2354 744 33401 2895 915 2.74 2.10* 0.01 Layer VI 20195 4607 1456 17994 3943 1247 1.14 2.10 0.26 * significant at 5% level Table 10 : Neuron density of different layers of temporal lobe of cerebral cortex in group I and group II. Group I Layers Group II Sample mean 38896 SD SE SD SE T –stat F-cal P 720 Sample mean 38571 2277 924 292 0.418 2.17 0.68 13119 3182 1006 11781 1252 396 1.23 2.17 0.23 Layer II 79140 8984 2841 69098 10540 3333 2.29 2.10* 0.03 Layer III 55266 5850 1850 65383 8989 2842 2.98 2.94** 0.009 Layer IV 35210 5289 1672 40664 6297 1991 2.10 2.10 0.05 Layer V 44084 3094 978 36295 2835 896 5.86 2.87** 1.49 Layer VI 24829 4278 1352 22552 3237 1023 1.34 2.10 0.19 Total Depth Layer I ** significant at 1% , * significant at 5% level Table 11 : Neuron density of different layers of occipital lobe of cerebral cortex in group I and group II. Group I Layers Group II Sample mean 40054 SD SE SD SE T –stat F-cal P 809 Sample mean 39843 2559 1820 575 0.212 2.11 0.83 13188 3481 1100 18771 3636 1149 3.507 2.87** 0.002 Layer II 68080 8658 2738 84310 8252 2609 4.290 2.87** 0.0004 Layer III 60328 4919 1555 57875 5204 1645 1.08 2.10 0.292 Layer IV 37366 4529 1432 51792 7047 2228 5.44 2.94** 6.80 Layer V 42694 2501 791 39680 4077 1289 1.99 2.13 0.06 Layer VI 24047 4250 1344 18321 3808 1204 3.17 2.87** 0.005 Total Depth Layer I ** significant at 1% , * significant at 5% level . Table 12: Total neuron count of different lobes of cerebral cortex in group I and group II Total Depth Lobes Group I Sample mean 661.4 Frontal lobe Temporal 681.5 lobe Occipital 687.3 lobe ** significant at 1% Group II SD SE T –stat F-cal P 9.12 Sample mean 612.8 25.92 8.19 3.96 2.87** 0.00 46.77 14.79 615.4 17.30 5.47 4.19 3.10** 0.00 50.58 15.99 664.8 30.79 9.73 1.20 2.13 0.24 SD SE 28.86 Table 13: Layer thickness of cerebellar cortex in group I and group II (µm). Group I Group II Layers Sample mean SD SE Sample mean SD SE T –stat F-cal P Molecular cell layer Purkinje 318.27 82.06 25.95 303.72 55.24 17.46 0.46 2.11 0.64 58.31 13.95 4.41 55.66 13.12 4.14 0.43 2.10 0.66 452.35 82.26 26.01 471.46 62.09 19.63 0.28 2.10 0.56 cell layer Granular cell layer Table 14: Neuronal count of different layers of cerebellar cortex in group I and group II. Group I Group II Layers Sample mean SD SE Sample mean SD SE T –stat F-cal P Molecular cell layer Purkinje 68.90 7.86 2.48 63.40 8.39 2.65 1.51 2.10 0.14 10.00 1.76 0.55 9.8 1.31 0.41 0.28 2.10 0.77 288.40 28.50 9.01 286.90 18.05 5.71 0.14 2.13 0.89 cell layer Granular cell layer Table 15: Neuronal density of different layers of cerebellar cortex in group I and group II. Group I Group II Layers Sample mean SD SE Sample mean SD SE T –stat F-cal P Molecular cell layer Purkinje 23204.84 7577 2396 21920 7058 2232 0.39 2.10 0.69 18670 7352 2325 18690.59 5598 1770 0.006 2.10 0.99 65379.71 11752 3716 61750.72 8585 2714 0.784 2.11 0.44 cell layer Granular cell layer Table 16: Neuron count, total neuron count and neuronal density in Pons in Group I and Group II Neuron count Group I Group II Statistic Total Neuron count Neuronal density Size S M L Mean 20 9.10 3.10 32.20 3220 SD 2.94 1.66 1.37 3.64 364.53 SE 0.93 0.52 0.43 1.15 115.27 Mean 17.80 9.00 2.60 29.40 2940 SD 2.57 1.94 1.07 2.87 287.51 SE 0.81 0.61 0.33 0.90 90.92 T –stat 1.77 0.12 0.90 1.90 1.90 F-cal 2.10 2.10 2.10 2.10 2.10 P value 0.09 0.90 0.37 0.07 0.07 Table 17: Neuron count, total neuron count and neuronal density in medulla oblongata in Group I and Group II Neuron count Group I Group II Statistic Total Neuron count Neuronal density Size S M L Mean 14.00 6.0 1.18 21.80 2180 SD 2.30 1.24 0.91 2.20 220.10 SE 0.73 0.39 0.29 0.69 69.60 Mean 13.40 4.90 1.70 20.00 2000 SD 3.16 1.19 0.67 3.62 362.09 SE 1.00 0.37 0.21 1.14 114.50 T –stat 0.48 2.01 0.27 1.34 1.34 F-cal 2.11 2.10 2.10 2.13 2.13 P value 0.63 0.05 0.78 0.19 0.19 Graph 1: Showing different indices of brain 160 140 120 100 Group I 80 Group II 60 40 20 0 Brain Index Cerebral Index Cerebellar Index Index Graph II: Showing thickness of different layers of frontal lobe 1800 1600 Thickness (µm) 1400 1200 1000 800 Group I 600 Group II 400 200 0 Total Depth I II III Cortical Layers IV V VI Graph III: Showing thickness of different layers of temporal lobe 1800 1600 Thickness (µm) 1400 1200 1000 800 Group I 600 Group II 400 200 0 Total Depth I II III IV V VI Cortical Layers Graph IV: Showing thickness of different layers of occipital lobe 1800 1600 Thickness (µm) 1400 1200 1000 800 Group I 600 Group II 400 200 0 Total Depth I II III Cortical Layers IV V VI Graph V: Showing neuronal density of different layers of frontal cortex 90000 80000 70000 50000 40000 Group I 30000 Group II 20000 10000 0 Total Depth I II III IV V VI Cortical Layers Graph VI: Showing neuronal density of different layers of temporal lobe 80000 70000 60000 Density Density 60000 50000 40000 30000 Group I 20000 Group II 10000 0 Total Depth I II III Cortical Layers IV V VI Graph VII: Showing neuronal density of different layers of occipital lobe 90000 80000 Neuronal density 70000 60000 50000 40000 Group I 30000 Group II 20000 10000 0 Total Depth I II III IV V VI Cortical layers Graph VIII: Showing total neuron count in different lobes 700 Total neuron count 680 660 640 Group I 620 Group II 600 580 560 Frontal lobe Temporal lobe Cerebral Lobes Occipital lobe Graph IX: Showing neuronal density of different layers of cerebellar cortex 70000 Neuron Density 60000 50000 40000 Group I 30000 Group II 20000 10000 0 Molecular cell layer Purkinje cell layer Granular cell layer Cerebellar cortex layer Graph X: Showing thickness of different layers of cerebellar cortex 500 450 Thickness (µm) 400 350 300 250 Group I 200 Group II 150 100 50 0 Molecular cell layer Purkinje cell layer Granular cell layer Cortical layers Graph XI: Showing neuron count in different layers of cerebellar cortex 300 200 150 Group I Group II 100 50 0 Molecular cell layer Purkinje cell layer Granular cell layer Cortical layers Graph XII: Showing neuronal density in Pons 3250 3200 Neuronal Density Neuron number 250 3150 3100 3050 Group I 3000 Group II 2950 2900 2850 2800 Group I Group II Graph XIII: Showing neuronal density in medulla oblongata 2200 Neuronal Density 2150 2100 Group I 2050 Group II 2000 1950 1900 Group I Group II BIBLIOGRAPHY Agashiwala, A. 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Dakshinkar Associate Dean, Nagpur Veterinary College, Nagpur ABSTRACT The present work is carried on 20 samples each of cerebrum, cerebellum, pons and medulla oblongata of goat. The biometrical, histological, histochemical, histoenzymic and electron microscopic observation of cerebrum, cerebellum, pons and medulla oblongata were recorded. The gross morphology indicated that cerebrum of goat was oblong to oval in shape. The cerebellum was oval along transverse axis. Pons was transversely elongated. The medulla oblongata continued as spinal cord. A highly significant statistical difference was observed in weight of whole brain with advancement of age. The microstructure of cerebral cortex, showed six layers. There was significant statistical difference in the cortical depth of cerebrum in Group I and Group II. Different shapes of neurons in cerebral cortex were recorded viz. spindle, pyramidal and stellate. The total number of neuron in frontal and temporal lobes of cerebral cortex showed highly significant statistical difference between Group I and Group II, but significant statistical decrease was recorded in Group I and Group II in temporal as well as in occipital lobe. It was noted that large sized neurons were absent in cerebral cortex in Group I as well as in Group II. Some neurons in molecular layer showed parallel orientation to surface and are called as ‘Cells of Cajal’. Significant statistical difference was found in thickness of external granular layer of cerebral cortex with advancement of age from Group I to Group II. The neurons in external granular layer were stellate shaped with centrally located nuclei. These neurons were arranged perpendicular to surface. A significant statistical difference was noticed in thickness of external pyramidal layer of cerebral cortex with advancement of age. The external pyramidal layer showed more population of medium and large size neurons than small size neurons in comparison to other layers. A significant statistical difference was observed in neuronal density of temporal lobe with advancement of age. A significant statistical difference was noted in the thickness of internal granular layer in cerebral cortex with advancement of age. The number of medium size neurons in internal granular layer of temporal lobe of cerebral cortex showed significant difference with advancement of age. The neuronal density in frontal and occipital lobes of internal granular layer of cerebral cortex showed significant statistical difference with advancement of age. A significant difference was recorded in thickness of internal pyramidal layer of temporal lobe in Group I and Group II. A significant statistical difference was noticed in number of large size neurons of internal pyramidal layer in frontal lobe of cerebral cortex with advancement of age. Neuronal density in frontal and temporal lobes of internal pyramidal layer of cerebral cortex showed significant statistical difference with advancement of age. A significant statistical difference was noticed between increase in thickness of multiform layer at frontal and occipital lobe of cerebral cortex with advancement of age. The neuronal density in multiform layer of occipital lobe of cerebral cortex showed significant statistical difference with advancement of age. The neuronal density in Purkinje cell layer of cerebellum increased with advancement of age from group I to group II. There was progressive decrease in density of granular cells of cerebellum from group I to group II with the advancement of age. There was reduction in number of neurons as well as density of neurons in medulla oblongata from group I to group II with advancement of age. The presence of glycogen in small neurons, large neurons, blood vessels and neuropil of cerebrum as well as in neuropil, molecular cells, Purkinje cells and granular cells of cerebellum increases with advancement of age from group I to group II. Pons and medulla oblongata showed weak to mild glycogen activity with advancement of age. The neurons, blood vessels and neuropil of cerebrum as well as cerebellum exhibited gradual increase in the acid phosphatase activity with the advancement of age. Pons and medulla oblongata showed intense activity in all cellular components. The alkaline phosphatases activity in small and large neurons, blood vessels and neuropil of cerebrum as well as cerebellum showed weak to mild activity whereas pons and medulla oblongata showed moderate alkaline phosphatase activity in their cellular components. Moderate to intense lipofuscin pigmentation was noted with advancement of age in cerebrum. It was also noted that due to heavy lipofuscin pigmentation, the nucleus of the large neuron was shifted toward periphery with advancement of age. Lipofuscin pigmentation was found to increase from weak to moderate in Purkinje cells, molecular cells and granular cells of cerebellum and also in neurons of pons and medulla oblongata. Electron microscopically cerebrum showed progressive granulation followed by mild pigmentation on myelin sheath. The myelin sheath was found to undergo splitting and cytoplasm of oligodendrocyte appeared as electron dense, which subsequently appeared as projected myelin balloons. Due to increase in size of myelin balloons, the vacuoles are formed and nucleus gets pushed towards one side. The neurons of the cerebellum were found to undergo complete degeneration with total shrinkage of mitochondria and cell was considered as apoptic with advancement of age. Electron microscopically, almost all the organelles of neuronal cells of pons exhibited disruption. The mitochondria appeared cloudy and myelin showed large vesicular appearance with very little cytoplasm with advancement of age. 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