Download Worksheet 6 - Iowa State University

Worksheet 6
Supplemental Instruction
Iowa State University
Leader:
Course:
Instructor:
Date:
Laura
Bio 313
Dr. Rodermel
9/22/15
WARM-UP:
Diagram Rolling-circle replication and show all the possible outcomes:
1. What kind of RNA is transcribed by the following polymerases?
a. RNA polymerase I
b. RNA polymerase II
c. RNA polymerase III
2. Draw the DNA Template with RNA being transcribed, be sure to include the Tx Bubble,
direction of synthesis, template and nontemplate strand
3. What is the PURPOSE of Initiation, Elongation, and Termination?
a. Initiation
b. Elongation
c. Termination
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4. How does sigma recognize the promoter? Can sigma always bind to the promoter?
5. What roll does conformational changes play in transcription? Why are they important?
6. How is the process of transcription similar and/or different between Prokaryotes and
Eukaryotes?
CHALLENGE QUESTIONS:
7. The following DNA nucleotides are found near the end of a bacterial transcription unit.
3’ – AGCATACAGCAGACCGTTGGTCTGAAAAAAGCATACA – 5’
a. Mark the point at which transcription will terminate
b. Is this terminator Rho dependent or Rho independent
c. Draw a diagram of the RNA that will be transcribed from this DNA, including it’s
nucleotide sequence and any secondary structure.
8. Suppose that the string of A nucleotides following the inverted repeat in a rhoindependent terminator were deleted but that the inverted repeat was left intact. How will
this deletion affect termination? What will happen when RNA polymerase reaches this
region?