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A SMN missense mutation complements SMN2 restoring snRNPs
A SMN missense mutation complements SMN2 restoring snRNPs

... mice that were heterozygous for the transgene, homozygous for SMN2 and homozygous for the mouse Smn knockout allele (SMN(VDQNQKE)þ/2 ; SMN2 þ/þ ; Smn2/2 ) or (SMN (A111G)þ/2 ; SMN2 þ/þ ; Smn2/2 ) were obtained. Expression of transgenes RT– PCR analysis revealed that the three SMN(VDQNQKE) lines had ...
Carnitine metabolism and biosynthesis in yeast Saccharomyces
Carnitine metabolism and biosynthesis in yeast Saccharomyces

... the distribution and flux of many important metabolic pathways, since many intermediates of metabolism can not easily cross compartmental membranes. In the case of energy metabolism, such membranes are impermeable to CoA-activated acyl residues, the primary source of energy production in respiratory ...
A Theory of Conceptual Advance: Explaining Conceptual Change in
A Theory of Conceptual Advance: Explaining Conceptual Change in

... of science. For conceptual problems have certain presuppositions which can be made explicit and understood only by studying the history of this problem (Lennox 2001a, 2001b). Contemporary debates and theoretical issues may have certain parallels to past debates and ideas that scientists have forgott ...
The monogenic primary dystonias
The monogenic primary dystonias

... postures (Fahn et al., 1987). Primary dystonias can be distinguished from secondary ones (Fahn et al., 1998; Geyer and Bressman, 2006). Primary forms solely or mainly present with dystonia. They are frequently inherited as monogenic traits and usually lack gross neuropathological changes. Dystonia p ...
Full-Text PDF
Full-Text PDF

... ricin and this work reported a molecular weight of approximately 62,766 Da [16]. In addition to analysis on purified ricin, matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS) of intact ricin was also reported using crude extracts from castor beans [17]. Altho ...
Determining the Transcription Factor Genes Populating a Fruit Fly
Determining the Transcription Factor Genes Populating a Fruit Fly

... The methodological goal of the RNAi screen was to reduce the expression of Drosophila melanogaster transcription factor genes individually along the dorsal midline of the abdomen. The reduction of gene expression occurs through the exploitation of the endogenous fruit fly RNA interference pathway. I ...
Nucleic acid vaccines for ehrlichia chaffeensis and methods of use
Nucleic acid vaccines for ehrlichia chaffeensis and methods of use

... disease caused by members of the tribe Ehrlichiae have been described. Ehrlichiae infect leukocytes and endothelial cells of many different mammalian species, some of them causing serious human and veterinary diseases. Over 400 cases of ...
TRANSLOCATIONS INVOLVING T H E THIRD AND THE FOURTH
TRANSLOCATIONS INVOLVING T H E THIRD AND THE FOURTH

... and noDeJoffspringwereproduced ...
PDF
PDF

... The mutant phenotype of gnom was first described by Jürgens et al. (Jürgens et al., 1991). gnom mutant embryos fail to properly form a root and shoot apical meristem, and bilateral symmetry is not established, resulting in a ball-shaped embryo. To better understand the origin of the patterning defec ...
Molecular characterization of a cluster of at least two
Molecular characterization of a cluster of at least two

... S. salivarius DNA and the isolation and characterization of the GTFpositive clones LA-8 and LA-33 have been described previously (Pitty et al., 1989). Bacteriophage L DNA was purified from 1 litre liquid lysates according to the method of Silhavy et al. (1984). Both the purification of plasmids from ...
Molecular characterization of a cluster of at least two
Molecular characterization of a cluster of at least two

... S. salivarius DNA and the isolation and characterization of the GTFpositive clones LA-8 and LA-33 have been described previously (Pitty et al., 1989). Bacteriophage L DNA was purified from 1 litre liquid lysates according to the method of Silhavy et al. (1984). Both the purification of plasmids from ...
Genetic polymorphisms among C57BL/6 mouse inbred strains
Genetic polymorphisms among C57BL/6 mouse inbred strains

... to these as ‘‘C57’’ or ‘‘C57 black’’ or ‘‘B6’’), it is important to distinguish the exact background when examining the genotype because of the possible effects on phenotype. Thus, knockout and transgenic mice produced in C57BL/6J and C57BL/6N inbred substrains might co-exist in a given animal house ...
Multiple sequence alignment for phylogenetic purposes
Multiple sequence alignment for phylogenetic purposes

... but instead establish homology between characters and their states. This is not a trivial semantic issue, because many of the algorithms used for sequence alignment have precious little relevance to establishing homology, even if they do line up the letters quite neatly. Putting biology into an alig ...
Analysis and engineering of acetyl
Analysis and engineering of acetyl

... acetyl-CoA synthesis is prodfft dependent. Moreofier, it demonstrated that, for some prodffts, it can be adfiantageoffs to combine mffltiple acetyl-CoA prodffction pathflays. To optimise a metabolic process sffch as cytosolic acetyl-CoA synthesis in yeast, ef cient and easy-to-ffse genetic engineering tools are ...
TaqMan® SNP Genotyping Assays User Guide
TaqMan® SNP Genotyping Assays User Guide

... Genotyping Assay overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 Assay setup guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Reagents and samples preparatio ...
VAAST 2 User`s Guide
VAAST 2 User`s Guide

... line.    This  header  line  begins  with  the  ‘>’  character.    The  first  contiguous  set  of  non-­‐ whitespace  characters  after  the  ‘>’  are  used  as  the  ID  of  that  sequence.    This  ID  must   match  EXACTLY  wi ...
Polyploidy
Polyploidy

... abnormal formation of vocal mechanisms. ...
2.1.databases_intro - T
2.1.databases_intro - T

... •Write down the PubMed Identifier (the number in the PMID field) of that interesting paper you just find. It could be very useful in your subsequent search for related items such as associated gene and protein sequences ...
Mechanisms underlying inhibition of muscle disuse
Mechanisms underlying inhibition of muscle disuse

... sequencing and functionally characterising its skeletal muscle transcriptome, and to conduct gene expression profiling to identify transcriptional pathways associated with metabolic depression and maintenance of muscle function in aestivating burrowing frogs. A transcriptome was assembled using next ...
The KetelD Dominant-Negative Mutations Identify
The KetelD Dominant-Negative Mutations Identify

... intriguing biological phenomena and raises questions about the origin and function of factors required for the initiation of a new life. What are those factors? Where and how are they made? What are their functions? It has long been known that most of the factors that are required during early embry ...
Unipro UGENE Manual
Unipro UGENE Manual

... Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched from the UGENE graphical interface, an external tool needs a corresponding executable file. The list of the external tools can be found on this page. The standard package does not include the tools, whereas ...
Unipro UGENE Manual
Unipro UGENE Manual

... Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched from the UGENE graphical interface, an external tool needs a corresponding executable file. The list of the external tools can be found on this page. The standard package does not include the tools, whereas ...
The Center for Organogenesis | University of Michigan, Ann Arbor
The Center for Organogenesis | University of Michigan, Ann Arbor

... cell fate decisions in numerous processes (Uwanogho et al., 1995). Sox1, Sox2 and Sox3 are all expressed in the lens (Kamachi et al., 1998). Sox2 and Sox3 expression in the early lens placode is dependent on the optic vesicle and this implies that they are responsive to inductive signals (Kamachi et ...
The Genetics of Migraine
The Genetics of Migraine

... disorder. Are the mechanisms of symptomatic migraine attacks different from those of primary migraine attacks? Or is it just that patients with these organic cerebral disorders have a lower threshold for developing migraine attacks? As described above, the IHS classification distinguishes different ...
Identification of Redeye, a new sleep-regulating protein whose
Identification of Redeye, a new sleep-regulating protein whose

... and iso31 genomes respectively. Following alignment with the published Drosophila genome, unique nucleotide polymorphisms present in rye mutants were pooled together as potential EMS-induced mutations (n = 26,224). Within the 11 Mb region identified through recombination mapping, there were 1457 pot ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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