Complete genome sequence of the prototype lactic acid bacterium
... the Staden package (86). The resulting draft assembly was then mapped on the genome of L. lactis IL1403 using the Projector software package (97) to order the contigs into large scaffolds. Standard PCR, followed by primer walk sequencing of the resulting products, was used to close the remaining 800 ...
... the Staden package (86). The resulting draft assembly was then mapped on the genome of L. lactis IL1403 using the Projector software package (97) to order the contigs into large scaffolds. Standard PCR, followed by primer walk sequencing of the resulting products, was used to close the remaining 800 ...
4. Rh Phenotyping
... 1. Place serum in a properly labeled tube (if not already done) and prepare a 4-6% red cell suspension for each patient in an appropriately labeled tube (refer to procedure 3). 2. Set up tubes for ABO and D type and an additional tube for each Rh typing sera (remember, anti-D is part of the forward ...
... 1. Place serum in a properly labeled tube (if not already done) and prepare a 4-6% red cell suspension for each patient in an appropriately labeled tube (refer to procedure 3). 2. Set up tubes for ABO and D type and an additional tube for each Rh typing sera (remember, anti-D is part of the forward ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... peptic diseases, gastric cancer and MALT-lymphoma [1]. There are many virulence factors for H. pylori colonization and infection but CagA is one of the most studied virulence of H. pylori. This toxin is encoded by cagA gene located on cagPAI, and protein CagA 120–145 kDa in size [2]. H. pylori strai ...
... peptic diseases, gastric cancer and MALT-lymphoma [1]. There are many virulence factors for H. pylori colonization and infection but CagA is one of the most studied virulence of H. pylori. This toxin is encoded by cagA gene located on cagPAI, and protein CagA 120–145 kDa in size [2]. H. pylori strai ...
Comparison of different PCR tests for detecting Shiga toxin
... reaction, with no need for any further processing of the samples and without opening the test tubes, greatly reducing the risk of carry-over contamination. Another advantage of real-time PCR methods is that they do not need ethidium bromide, which is subject to strict and constraining regulations in ...
... reaction, with no need for any further processing of the samples and without opening the test tubes, greatly reducing the risk of carry-over contamination. Another advantage of real-time PCR methods is that they do not need ethidium bromide, which is subject to strict and constraining regulations in ...
Fulltext PDF - Indian Academy of Sciences
... stambh A locus of Drosophila melanogaster. stmA is localized to polytene bands 44D1.2 on 2R. stmA mutations are of two types: temperature-sensitive (ts) adult and larval paralytic or unconditional embryonic or larval lethal. Twelve alleles reported in this study fall into two intragenic complementin ...
... stambh A locus of Drosophila melanogaster. stmA is localized to polytene bands 44D1.2 on 2R. stmA mutations are of two types: temperature-sensitive (ts) adult and larval paralytic or unconditional embryonic or larval lethal. Twelve alleles reported in this study fall into two intragenic complementin ...
GENETICS DEFINITION
... Select differently depending on type. Additive: identify the best and breed best to best. Non-additive: easiest to outbreed or crossbreed. ...
... Select differently depending on type. Additive: identify the best and breed best to best. Non-additive: easiest to outbreed or crossbreed. ...
Characterization of an Immuno-dominant Variable Surface
... region of both actin and ferredoxin genes (Fig . 5), the only other genes of E. histolytica where sequence has been determined. The transcriptional start site of M17 was mapped to an adenine residue 17 by 5' of the start codon by primer extension sequence analysis using oligonucleotide SR09 (data no ...
... region of both actin and ferredoxin genes (Fig . 5), the only other genes of E. histolytica where sequence has been determined. The transcriptional start site of M17 was mapped to an adenine residue 17 by 5' of the start codon by primer extension sequence analysis using oligonucleotide SR09 (data no ...
Assessing the role of anammox in a nitrogen contaminated aquifer
... Dissimilatory reduction of nitrate to ammonium (DNRA) process occurs in low oxygen settings similar to denitrification, and both processes can even compete for available nitrate. This process is mediated by prokaryotes carrying the nrfA gene. (See review: Simon 2002). One of the main concerns about ...
... Dissimilatory reduction of nitrate to ammonium (DNRA) process occurs in low oxygen settings similar to denitrification, and both processes can even compete for available nitrate. This process is mediated by prokaryotes carrying the nrfA gene. (See review: Simon 2002). One of the main concerns about ...
Metabolic engineering of Escherichia coli and Corynebacterium glutamicum for the -threonine
... impetus to further improve productivity of the bioconversion and reduce its costs. The most efficient solution is to develop more productive bacterial strains with reduced growth retardation. Strains surviving multiple rounds of mutation are genetically undefined and vulnerable to further changes, occ ...
... impetus to further improve productivity of the bioconversion and reduce its costs. The most efficient solution is to develop more productive bacterial strains with reduced growth retardation. Strains surviving multiple rounds of mutation are genetically undefined and vulnerable to further changes, occ ...
The growth hormone auxin
... Auxin biosynthesis 1. Necessary for determining the physiological roles of auxin 2. Necessary for understanding auxin movements and dynamics 3. Provides genetic foundations for dissecting the mechanisms of auxin in plant development ...
... Auxin biosynthesis 1. Necessary for determining the physiological roles of auxin 2. Necessary for understanding auxin movements and dynamics 3. Provides genetic foundations for dissecting the mechanisms of auxin in plant development ...
Nectary formation is ABC independent - Development
... occupy lateral positions and four medial positions. The medial glands are oblong and smaller than the lateral ones. The lateral glands consist of two oblong confluent parts or are sometimes large and disk-shaped, the width of which is slightly greater than the diameter of the stamen filaments (prese ...
... occupy lateral positions and four medial positions. The medial glands are oblong and smaller than the lateral ones. The lateral glands consist of two oblong confluent parts or are sometimes large and disk-shaped, the width of which is slightly greater than the diameter of the stamen filaments (prese ...
Investigating genotype-phenotype relationships in
... can cause the re-adjustment of fluxes distributed around important metabolic branches. Furthermore, this study demonstrates not only the need for development of new computational tools accounting for regulatory targets for metabolic engineering, but also the need for better understanding the relatio ...
... can cause the re-adjustment of fluxes distributed around important metabolic branches. Furthermore, this study demonstrates not only the need for development of new computational tools accounting for regulatory targets for metabolic engineering, but also the need for better understanding the relatio ...
The Bacterial Toxin RelE Displays Codon
... At a RelE concentration four times less than the concentration of ribosomes that were competent in dipeptide formation (Zavialov et al., 2001; Experimental Procedures), peptide synthesis from the mRNA with UAG as stop codon was completely blocked by the toxin. With twenty times less RelE than active ...
... At a RelE concentration four times less than the concentration of ribosomes that were competent in dipeptide formation (Zavialov et al., 2001; Experimental Procedures), peptide synthesis from the mRNA with UAG as stop codon was completely blocked by the toxin. With twenty times less RelE than active ...
Unequal Crossing-over in Unique PABP2 Mutations in Japanese
... We analyzed PABP2 in both index patients and the 2 siblings of patient A after obtaining informed consent. ...
... We analyzed PABP2 in both index patients and the 2 siblings of patient A after obtaining informed consent. ...
The role of Grainy head in epithelial tissue growth
... The grainy head (grh) gene family encodes an important group of transcription factors that play a remarkably conserved role in epithelial organ development, epithelial barrier formation and epithelial repair upon damage in different organisms. The regulation and molecular targets of Grh are numerous ...
... The grainy head (grh) gene family encodes an important group of transcription factors that play a remarkably conserved role in epithelial organ development, epithelial barrier formation and epithelial repair upon damage in different organisms. The regulation and molecular targets of Grh are numerous ...
Sucrose breakdown in the potato tuber - publish.UP
... In this work different approaches are undertaken to improve the understanding of the sucroseto-starch pathway in developing potato tubers. At first an inducible gene expression system from fungal origin is optimised for the use of studying metabolism in the potato tuber. It is found that the alc sys ...
... In this work different approaches are undertaken to improve the understanding of the sucroseto-starch pathway in developing potato tubers. At first an inducible gene expression system from fungal origin is optimised for the use of studying metabolism in the potato tuber. It is found that the alc sys ...
Time to asthma onset Asthma score Bivariate analysis - Hal-CEA
... Linkage analysis of the martingale residuals was performed using the Maximum Likelihood Binomial method (Abel and Muller-Myhsok 1998; Alcais and Abel 1999), as implemented in MLB-GENEHUNTER (Abel and Muller-Myhsok 1998). As opposed to the Variance Components (VC) method, this method does not requir ...
... Linkage analysis of the martingale residuals was performed using the Maximum Likelihood Binomial method (Abel and Muller-Myhsok 1998; Alcais and Abel 1999), as implemented in MLB-GENEHUNTER (Abel and Muller-Myhsok 1998). As opposed to the Variance Components (VC) method, this method does not requir ...
Molecular and biochemical characterization of cytosolic
... ADP-glucose (ADPG) can be synthesized in the cytosol and taken up directly by the amyloplast as well as being synthesized within the organelle. The possession of a cytosolic AGPase could bypass the need for the G1P produced by the plastidial PGM reaction, since ADPG synthesis could be directly linke ...
... ADP-glucose (ADPG) can be synthesized in the cytosol and taken up directly by the amyloplast as well as being synthesized within the organelle. The possession of a cytosolic AGPase could bypass the need for the G1P produced by the plastidial PGM reaction, since ADPG synthesis could be directly linke ...
allJoiner
... appears in fields of multiple tables, sometimes even multiple databases. • $gbd is a variable that contains a comma-separated list of genome databases. • An identifier consists of a an identifier line, a required comment in quotes, and a list of database.table.field where the identifier is used. The ...
... appears in fields of multiple tables, sometimes even multiple databases. • $gbd is a variable that contains a comma-separated list of genome databases. • An identifier consists of a an identifier line, a required comment in quotes, and a list of database.table.field where the identifier is used. The ...
The one-eyed pinhead gene functions in mesoderm and endoderm
... RAPD PCR assays were performed as described by Postlethwait et al. (1994). In initial experiments, the RAPD marker 15AH.500 was found to be linked to oep. Since RAPD markers are strain-specific and therefore not informative in all mapping crosses, a sequence-tagged site (STS) marker for the 15AH.500 ...
... RAPD PCR assays were performed as described by Postlethwait et al. (1994). In initial experiments, the RAPD marker 15AH.500 was found to be linked to oep. Since RAPD markers are strain-specific and therefore not informative in all mapping crosses, a sequence-tagged site (STS) marker for the 15AH.500 ...
Hermann Joseph Muller - National Academy of Sciences
... Sturtevant used the data from Morgan’s first X-linked mutations and constructed a map. Muller was in awe of Sturtevant’s interpretation. (Sturtevant was still an undergraduate when he created the first map). Muller suggested using the ratio of crossovers to the total of crossovers and noncrossovers ...
... Sturtevant used the data from Morgan’s first X-linked mutations and constructed a map. Muller was in awe of Sturtevant’s interpretation. (Sturtevant was still an undergraduate when he created the first map). Muller suggested using the ratio of crossovers to the total of crossovers and noncrossovers ...
gemini Documentation
... While other tools (e.g., curl) can be used to download this file, wget is required by the installation script. Please install wget if it is not already available: Once you have downloaded the above installation script, you can proceed as follows: $ python gemini_install.py /usr/local /usr/local/shar ...
... While other tools (e.g., curl) can be used to download this file, wget is required by the installation script. Please install wget if it is not already available: Once you have downloaded the above installation script, you can proceed as follows: $ python gemini_install.py /usr/local /usr/local/shar ...
Biosynthesis of geranial, a potent aroma compound in ginger
... experiments using deuterium-labeled geraniol and geranyl acetate. GC-MS analysis revealed that the incorporated geraniol and geranyl acetate were transformed and detected as geranial, geraniol, geranyl acetate and citronellol; however, nerol and neral were hardly detected. Next, we isolated and char ...
... experiments using deuterium-labeled geraniol and geranyl acetate. GC-MS analysis revealed that the incorporated geraniol and geranyl acetate were transformed and detected as geranial, geraniol, geranyl acetate and citronellol; however, nerol and neral were hardly detected. Next, we isolated and char ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.