Chance and Necessity in Arthur Peacocke`s Scientific Work
... time understood it. Such thoughts must have started to be formed in the mind of Peacocke and lead him on the path of pondering the nature of the interaction of living matter with the non-living world and communion of the whole of creation. At the very root of his subsequent deep thoughts on the natu ...
... time understood it. Such thoughts must have started to be formed in the mind of Peacocke and lead him on the path of pondering the nature of the interaction of living matter with the non-living world and communion of the whole of creation. At the very root of his subsequent deep thoughts on the natu ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... rate-limiting enzymes of polyamine biosynthesis can increase by orders of magnitude. Growth regulation by polyamines may be related in part to interactions with nucleic acids. Early evidence for this interaction emphasized the ability of polyamines to alter DNA conformation by condensation and aggre ...
... rate-limiting enzymes of polyamine biosynthesis can increase by orders of magnitude. Growth regulation by polyamines may be related in part to interactions with nucleic acids. Early evidence for this interaction emphasized the ability of polyamines to alter DNA conformation by condensation and aggre ...
Inhibition of RNA Synthesis by Anthracycline Analogs
... of anthracyclines in the treatment of leukemias and other forms of cancer. The biological and pharmacological activities of these drugs appear to be, at least in part, due to their ability to interact with DNA. ...
... of anthracyclines in the treatment of leukemias and other forms of cancer. The biological and pharmacological activities of these drugs appear to be, at least in part, due to their ability to interact with DNA. ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... with cold 0.3 ~ TCA. 1 rag. thymus DNA and 2 rag. serum albumin were added before the first precipitation, and small amounts of additional serum albumin were added at each subsequent washing and precipitation, to ensure rapid flocculation and tight packing of the sediments. The washed precipitate wa ...
... with cold 0.3 ~ TCA. 1 rag. thymus DNA and 2 rag. serum albumin were added before the first precipitation, and small amounts of additional serum albumin were added at each subsequent washing and precipitation, to ensure rapid flocculation and tight packing of the sediments. The washed precipitate wa ...
![arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007](http://s1.studyres.com/store/data/014946021_1-c477dba1add7a260e278ca181f537c79-300x300.png)
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... medicine is now at the forefront of scientific research. To realize this potential, however, we need a revolutionary sequencing method for the cost-effective and rapid interrogation of individual genomes. This capability is likely to be provided by a physical approach to probing DNA at the single nu ...
... medicine is now at the forefront of scientific research. To realize this potential, however, we need a revolutionary sequencing method for the cost-effective and rapid interrogation of individual genomes. This capability is likely to be provided by a physical approach to probing DNA at the single nu ...
Investigating the DNA-Binding Interactions of Small
... thank the Goodson Group members for their help and support. I consider the Goodson group to be family. I would also like to thank my collaborators (Prof. Wilson, Prof. Imperiali, Prof. Laine) and dissertation committee members (Prof. Chen, Prof. Kuroda, Prof. Robertson). Finally, I would like to tha ...
... thank the Goodson Group members for their help and support. I consider the Goodson group to be family. I would also like to thank my collaborators (Prof. Wilson, Prof. Imperiali, Prof. Laine) and dissertation committee members (Prof. Chen, Prof. Kuroda, Prof. Robertson). Finally, I would like to tha ...
Defining characteristics of Tn5 Transposase non
... While non-specific DNA plays a role in target localization for many recombinases, transcription factors and restriction enzymes, the importance of nonspecific DNA interactions for transposases has not been investigated. Here, we discuss non-specific DNA-Tn5 Transposase (Tnp) interactions and suggest ...
... While non-specific DNA plays a role in target localization for many recombinases, transcription factors and restriction enzymes, the importance of nonspecific DNA interactions for transposases has not been investigated. Here, we discuss non-specific DNA-Tn5 Transposase (Tnp) interactions and suggest ...
Press release
... protocol and secondly also on the starting weight or volume. It also depends to a large extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which i ...
... protocol and secondly also on the starting weight or volume. It also depends to a large extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which i ...
2. Assessing the probative value of DNA evidence
... Using the Four Practitioner Guides – Notes, Caveats and Disclaimers The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to functi ...
... Using the Four Practitioner Guides – Notes, Caveats and Disclaimers The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to functi ...
Assessing the Probative Value of DNA Evidence
... Using the Four Practitioner Guides – Notes, Caveats and Disclaimers The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to functi ...
... Using the Four Practitioner Guides – Notes, Caveats and Disclaimers The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to functi ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... the plasmid do not share homology with the yeast genome, circular inverted (head-to-head) dimer plasmids are theprincipal product of repair. By measurements of the DNA concentration dependence of transformation with alinearized plasmid, and by transformation with mixtures of genetically marked plasm ...
... the plasmid do not share homology with the yeast genome, circular inverted (head-to-head) dimer plasmids are theprincipal product of repair. By measurements of the DNA concentration dependence of transformation with alinearized plasmid, and by transformation with mixtures of genetically marked plasm ...
Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... ratio, its execution time is too high for long sequences. DNACompress (Chen et al., 2002) consists of two parts. All approximate repeats including complementary palindromes are detected by a separate software tool called PatternHunter (Ma et al., 2002) in the first part. Those approximate repeats an ...
... ratio, its execution time is too high for long sequences. DNACompress (Chen et al., 2002) consists of two parts. All approximate repeats including complementary palindromes are detected by a separate software tool called PatternHunter (Ma et al., 2002) in the first part. Those approximate repeats an ...
Analysis of clones carrying repeated DNA sequences in two YAC
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
2- pcr primer design and reaction optimisation
... Thus, the annealing temperature chosen for a PCR depends directly on length and composition of the primer(s). One should aim at using an annealing temperature (Ta) about 5oC below the lowest Tm of the pair of primers to be used (Innis and Gelfand, 1990). A more rigorous treatment of Ta is given by R ...
... Thus, the annealing temperature chosen for a PCR depends directly on length and composition of the primer(s). One should aim at using an annealing temperature (Ta) about 5oC below the lowest Tm of the pair of primers to be used (Innis and Gelfand, 1990). A more rigorous treatment of Ta is given by R ...
Graph-based Methods for the Design of DNA - TUBdok
... evaluations of several DNA word sets from the literature is performed. 2. A new approach is developed for solving a minimal free energy (MFE) problem for the hybridization complex built by a pair of partially complementary DNA molecules. It introduces a concept of the hybridization graph to represen ...
... evaluations of several DNA word sets from the literature is performed. 2. A new approach is developed for solving a minimal free energy (MFE) problem for the hybridization complex built by a pair of partially complementary DNA molecules. It introduces a concept of the hybridization graph to represen ...
Equilibrium Statistics of Channel-confined DNA
... of different experimental techniques [31, 33], showing that it is a good model of DNA, except when subjected to very high forces [34, 35], or in the presence of positive polyvalent ions [36]. The contour length can be directly computed from the lateral distance between base pairs as L = (Number of b ...
... of different experimental techniques [31, 33], showing that it is a good model of DNA, except when subjected to very high forces [34, 35], or in the presence of positive polyvalent ions [36]. The contour length can be directly computed from the lateral distance between base pairs as L = (Number of b ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... on DNA and RNA can optimize the use of these compounds and their analogues as medical drugs. Hoechst dyes 33342 and 33258 are closely related bisbenzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl)-5[5-(4-methylpiperazine-1-yl) benzimidazo-2-yl]-benzimidazole. Hoechst 33342 contains additional ethyl gr ...
... on DNA and RNA can optimize the use of these compounds and their analogues as medical drugs. Hoechst dyes 33342 and 33258 are closely related bisbenzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl)-5[5-(4-methylpiperazine-1-yl) benzimidazo-2-yl]-benzimidazole. Hoechst 33342 contains additional ethyl gr ...
Assessment by Molecular Dynamics Simulations of the Structural
... typically binds three successive base-pairs of DNA sequence. For a typical zinc finger, it is well established that the majority of contacts are between the side-chains of helical residues 2 1 (the residue immediately preceding the a-helix), 3 and 6 with the G-rich strand (primary strand), whose 50 ...
... typically binds three successive base-pairs of DNA sequence. For a typical zinc finger, it is well established that the majority of contacts are between the side-chains of helical residues 2 1 (the residue immediately preceding the a-helix), 3 and 6 with the G-rich strand (primary strand), whose 50 ...
Complete
... physical model for explaining the complicated separation characteristics of DNA prisms is developed. The model assumes that DNA molecules in such devices act as damped springs, which change their lengths in response to the applied electric pulses, instead of a rigid chain, as assumed in prior works. ...
... physical model for explaining the complicated separation characteristics of DNA prisms is developed. The model assumes that DNA molecules in such devices act as damped springs, which change their lengths in response to the applied electric pulses, instead of a rigid chain, as assumed in prior works. ...
pyrimidine
... An automated sequencing machine can handle 4500 bases/hour That’s one of the technologies that has made large-scale sequencing projects like the human genome project possible ...
... An automated sequencing machine can handle 4500 bases/hour That’s one of the technologies that has made large-scale sequencing projects like the human genome project possible ...
Charge Transport in DNA - Insights from
... based on thermodynamic observations.[26] Since then, reports have been constantly appearing against,[27] as well as in favor of the thermally induced melting.[28] Importantly, the nature of the overstretched state of DNA probably depends on the rate of stretching. A dsDNA oligomer was reported to de ...
... based on thermodynamic observations.[26] Since then, reports have been constantly appearing against,[27] as well as in favor of the thermally induced melting.[28] Importantly, the nature of the overstretched state of DNA probably depends on the rate of stretching. A dsDNA oligomer was reported to de ...
RHD - Labex
... Maternal cell-free DNA in plasma of pregnant women consists of longer fragments than in non pregnant women ...
... Maternal cell-free DNA in plasma of pregnant women consists of longer fragments than in non pregnant women ...
the association of chloroplast dna with photosynthetic membrane
... The chloroplast vesicles can be fixed and processed gently with minimal manipulation for light-microscope autoradiography. When the vesicles dry down on to the slide only the grana rich regions can be readily visualized and the stroma appears clear. Observations indicate that the grana rich regions ...
... The chloroplast vesicles can be fixed and processed gently with minimal manipulation for light-microscope autoradiography. When the vesicles dry down on to the slide only the grana rich regions can be readily visualized and the stroma appears clear. Observations indicate that the grana rich regions ...
Recombinant DNA technology and molecular cloning
... coli host strain K-12 is infected by phage λ·K, the phage DNA is not recognized as foreign because it has the same methylation pattern as the E. coli host genome. When the phage DNA replicates, the newly replicated DNA is modified by a specific methylase to maintain the pattern. Methylated DNA is no ...
... coli host strain K-12 is infected by phage λ·K, the phage DNA is not recognized as foreign because it has the same methylation pattern as the E. coli host genome. When the phage DNA replicates, the newly replicated DNA is modified by a specific methylase to maintain the pattern. Methylated DNA is no ...
DNA
Deoxyribonucleic acid (/diˌɒksiˌraɪbɵ.njuːˌkleɪ.ɨk ˈæsɪd/; DNA) is a molecule that carries most of the genetic instructions used in the development, functioning and reproduction of all known living organisms and many viruses. DNA is a nucleic acid; alongside proteins and carbohydrates, nucleic acids compose the three major macromolecules essential for all known forms of life. Most DNA molecules consist of two biopolymer strands coiled around each other to form a double helix. The two DNA strands are known as polynucleotides since they are composed of simpler units called nucleotides. Each nucleotide is composed of a nitrogen-containing nucleobase—either cytosine (C), guanine (G), adenine (A), or thymine (T)—as well as a monosaccharide sugar called deoxyribose and a phosphate group. The nucleotides are joined to one another in a chain by covalent bonds between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone. According to base pairing rules (A with T, and C with G), hydrogen bonds bind the nitrogenous bases of the two separate polynucleotide strands to make double-stranded DNA. The total amount of related DNA base pairs on Earth is estimated at 5.0 x 1037, and weighs 50 billion tonnes. In comparison, the total mass of the biosphere has been estimated to be as much as 4 TtC (trillion tons of carbon).DNA stores biological information. The DNA backbone is resistant to cleavage, and both strands of the double-stranded structure store the same biological information. Biological information is replicated as the two strands are separated. A significant portion of DNA (more than 98% for humans) is non-coding, meaning that these sections do not serve as patterns for protein sequences.The two strands of DNA run in opposite directions to each other and are therefore anti-parallel. Attached to each sugar is one of four types of nucleobases (informally, bases). It is the sequence of these four nucleobases along the backbone that encodes biological information. Under the genetic code, RNA strands are translated to specify the sequence of amino acids within proteins. These RNA strands are initially created using DNA strands as a template in a process called transcription.Within cells, DNA is organized into long structures called chromosomes. During cell division these chromosomes are duplicated in the process of DNA replication, providing each cell its own complete set of chromosomes. Eukaryotic organisms (animals, plants, fungi, and protists) store most of their DNA inside the cell nucleus and some of their DNA in organelles, such as mitochondria or chloroplasts. In contrast, prokaryotes (bacteria and archaea) store their DNA only in the cytoplasm. Within the chromosomes, chromatin proteins such as histones compact and organize DNA. These compact structures guide the interactions between DNA and other proteins, helping control which parts of the DNA are transcribed.First isolated by Friedrich Miescher in 1869 and with its molecular structure first identified by James Watson and Francis Crick in 1953, DNA is used by researchers as a molecular tool to explore physical laws and theories, such as the ergodic theorem and the theory of elasticity. The unique material properties of DNA have made it an attractive molecule for material scientists and engineers interested in micro- and nano-fabrication. Among notable advances in this field are DNA origami and DNA-based hybrid materials.The obsolete synonym ""desoxyribonucleic acid"" may occasionally be encountered, for example, in pre-1953 genetics.