7.013 Spring 2005 Problem Set 1Solutions
... d) Gln12 is the 12th amino acid in primary sequence. Val 98 is the 98th amino acid in the primary sequence. Explain how these amino acids are far apart in the primary sequence of the protein yet are close to each other in the region of the protein diagrammed above. When the protein folds into its fi ...
... d) Gln12 is the 12th amino acid in primary sequence. Val 98 is the 98th amino acid in the primary sequence. Explain how these amino acids are far apart in the primary sequence of the protein yet are close to each other in the region of the protein diagrammed above. When the protein folds into its fi ...
Facile and green production of aqueous graphene dispersions for
... Graphene consists of sp2-hybridized carbon atoms that form a one-atom-thick, two-dimensional honeycomb-like hexagonal structure. Graphene was discovered in 20041 and since then has found many applications in nanoscience and nanotechnology.2 In particular, graphene has emerged as a functional materia ...
... Graphene consists of sp2-hybridized carbon atoms that form a one-atom-thick, two-dimensional honeycomb-like hexagonal structure. Graphene was discovered in 20041 and since then has found many applications in nanoscience and nanotechnology.2 In particular, graphene has emerged as a functional materia ...
Intra- and Intergeneric Similarities of Pseudomonas and
... marginata, Pseudomonas caryophylli, and Pseudomonas lemoignei. Each of these rRNA branches is as heterogeneous as a genus. The Pseudomonas solanacearum and Pseudomonas acidovorans rRNA branches are about as far removed from each other as they are from the genera Janthinobacterium and Derxia and the ...
... marginata, Pseudomonas caryophylli, and Pseudomonas lemoignei. Each of these rRNA branches is as heterogeneous as a genus. The Pseudomonas solanacearum and Pseudomonas acidovorans rRNA branches are about as far removed from each other as they are from the genera Janthinobacterium and Derxia and the ...
No Slide Title
... suffering from HFI is to have one of two tests: 1) An enzymatic assay to determine aldolase activity. The aldolase is obtained from patient liver tissue in an invasive surgical procedure called a liver biopsy. 2) A fructose tolerance test. Fructose is injected intravenously under controlled conditio ...
... suffering from HFI is to have one of two tests: 1) An enzymatic assay to determine aldolase activity. The aldolase is obtained from patient liver tissue in an invasive surgical procedure called a liver biopsy. 2) A fructose tolerance test. Fructose is injected intravenously under controlled conditio ...
University of Groningen Citrate driven transamination for
... metabolize citrate and is hardly found in other environments. The genes encoding the citrate metabolic pathway are located on the chromosome, in contrast to the gene encoding the citrate uptake system that is encoded on plasmids. The widely studied and sequenced strain IL1403 belongs to this group a ...
... metabolize citrate and is hardly found in other environments. The genes encoding the citrate metabolic pathway are located on the chromosome, in contrast to the gene encoding the citrate uptake system that is encoded on plasmids. The widely studied and sequenced strain IL1403 belongs to this group a ...
Mechanism of Posttranslational Regulation of Phenol
... a sulfuryl group transfer (16). Routine analysis of the activity of sulfotransferase generally measures only the physiological activity (11, 12) catalyzed by β-form PST, and the R-form activity was mostly ignored (15, 16). The role of the two enzyme forms in physiological condition is yet to be stud ...
... a sulfuryl group transfer (16). Routine analysis of the activity of sulfotransferase generally measures only the physiological activity (11, 12) catalyzed by β-form PST, and the R-form activity was mostly ignored (15, 16). The role of the two enzyme forms in physiological condition is yet to be stud ...
1. Introduction to Natural Products Chemistry
... • Some animals, notably some soft corals and frogs produce highly bioactive alkaloids. In some cases, however, the alkaloids were found to have been ingested in the diet by the organism and then modified for use. Well-known examples are the pyrrolizidine alkaloids in caterpillars and moths. Alkaloid ...
... • Some animals, notably some soft corals and frogs produce highly bioactive alkaloids. In some cases, however, the alkaloids were found to have been ingested in the diet by the organism and then modified for use. Well-known examples are the pyrrolizidine alkaloids in caterpillars and moths. Alkaloid ...
glucagon superfamily peptides exhibiting nuclear hormone
... CROSS REFERENCE TO RELATED APPLICATIONS ...
... CROSS REFERENCE TO RELATED APPLICATIONS ...
Organic Acid
... is colored red. 3 - Application in food Organic acids are used in food preservation because of their effects on bacteria. The key basic principle on the mode of action of organic acids on bacteria is that non-dissociated (non-ionized) organic acids can penetrate the bacteria cell wall and disrupt th ...
... is colored red. 3 - Application in food Organic acids are used in food preservation because of their effects on bacteria. The key basic principle on the mode of action of organic acids on bacteria is that non-dissociated (non-ionized) organic acids can penetrate the bacteria cell wall and disrupt th ...
Multiple sequence alignment for phylogenetic purposes
... pairwise amino-acid identity among the six full sequences is 21%, which is within what is known as the ‘twilight zone’ of protein similarity, where sequence alignment can be very problematic. The two most different sequences, human hydroxyacylglutathione hydrolase (or glyoxalase II) (labelled 1qh5A ...
... pairwise amino-acid identity among the six full sequences is 21%, which is within what is known as the ‘twilight zone’ of protein similarity, where sequence alignment can be very problematic. The two most different sequences, human hydroxyacylglutathione hydrolase (or glyoxalase II) (labelled 1qh5A ...
Methods and strategies of peptide ligation
... CT-COOH), the functional group can be placed either at the side chain or at the ␣-amine (or as an ester). A common combination for an NT-amine-nucleophile is to place the nucleophile at the side chain of an NT-amino acid. This spatial configuration is found in many naturally occurring amino acids. E ...
... CT-COOH), the functional group can be placed either at the side chain or at the ␣-amine (or as an ester). A common combination for an NT-amine-nucleophile is to place the nucleophile at the side chain of an NT-amino acid. This spatial configuration is found in many naturally occurring amino acids. E ...
Identification of Modified Amino Acids by Edman Sequencing
... Biomolecular Resource Facilities (ABRF) has directed numerous studies focused on various aspects of Edman degradation of proteins and peptides. These studies provide a means for participating laboratories to compare their analyses against a benchmark of those from other laboratories that provide thi ...
... Biomolecular Resource Facilities (ABRF) has directed numerous studies focused on various aspects of Edman degradation of proteins and peptides. These studies provide a means for participating laboratories to compare their analyses against a benchmark of those from other laboratories that provide thi ...
INVESTIGATION OF THE REPLACEMENT OF CYSTEINE
... never reached these heights without the support, encouragement and guidance of several individuals. Therefore, I would like to acknowledge all the people whose support, patience and efforts have resulted in this dissertation. I would like to thank my advisor, Dr. Susan Lever f ...
... never reached these heights without the support, encouragement and guidance of several individuals. Therefore, I would like to acknowledge all the people whose support, patience and efforts have resulted in this dissertation. I would like to thank my advisor, Dr. Susan Lever f ...
Amino Acid-Protecting Groups
... - α,α-Dimethyl-3,5-dimethoxybenzyloxycarbonyl (Ddz).36 Although Ddz is more acid-stable than the Bpoc and the Trt groups, its removal with 1-5% TFA in DCM makes it compatible with t Bu-type side chain protection.,37 It can also be removed by photolysis at wavelengths above 280 nm,36 which makes it p ...
... - α,α-Dimethyl-3,5-dimethoxybenzyloxycarbonyl (Ddz).36 Although Ddz is more acid-stable than the Bpoc and the Trt groups, its removal with 1-5% TFA in DCM makes it compatible with t Bu-type side chain protection.,37 It can also be removed by photolysis at wavelengths above 280 nm,36 which makes it p ...
Rat sn-glycerol-3-phosphate acyltransferase - UNC
... with EcoRI to give two fragments of 0.8 and 0.9 kb. The 0.8 kb fragment, which contained DNA homologous to the coding region from the mouse GPAT cDNA sequence, was random prime labeled with [32 P]dCTP and used to probe a liver cDNA library prepared from 7 days old rats [19,20]. A 4.6 kb clone was is ...
... with EcoRI to give two fragments of 0.8 and 0.9 kb. The 0.8 kb fragment, which contained DNA homologous to the coding region from the mouse GPAT cDNA sequence, was random prime labeled with [32 P]dCTP and used to probe a liver cDNA library prepared from 7 days old rats [19,20]. A 4.6 kb clone was is ...
Mechanism of citric acid accumulation by Aspergillus niger in solid
... of the enzymes of interest. It was concluded that internal accumulation of citrate or oxalate is an immediate cause of its excretion. Supplemented by evidence from the ratio of ATP/AMP in the cells, a complete hypothesis describing citrate accumulation in A .niger Yang No.2 and MH 15- 15 is proposed ...
... of the enzymes of interest. It was concluded that internal accumulation of citrate or oxalate is an immediate cause of its excretion. Supplemented by evidence from the ratio of ATP/AMP in the cells, a complete hypothesis describing citrate accumulation in A .niger Yang No.2 and MH 15- 15 is proposed ...
Characterization of an Immuno-dominant Variable Surface
... was fractionated by preparative 5-15% gradient SDS-PAGE . After electrophoretic transfer to nitrocellulose the 125-kD region was excised from the blot, ground to a powder, and suspended in PBS. 100 141 of the suspension were diluted 1:9 with PBS and injected three times intraperitoneally into mice a ...
... was fractionated by preparative 5-15% gradient SDS-PAGE . After electrophoretic transfer to nitrocellulose the 125-kD region was excised from the blot, ground to a powder, and suspended in PBS. 100 141 of the suspension were diluted 1:9 with PBS and injected three times intraperitoneally into mice a ...
Classification and Nomenclature of Amines
... • Salts of amines are generally white crystalline solids with high melting points. ...
... • Salts of amines are generally white crystalline solids with high melting points. ...
Chapter 27 Amino acid
... The measured pKa of glycine is 2.34. Glycine is stronger than a typical carboxylic acid because the positively charged N acts as an electron-withdrawing, acid-strengthening substituent on the carbon. ...
... The measured pKa of glycine is 2.34. Glycine is stronger than a typical carboxylic acid because the positively charged N acts as an electron-withdrawing, acid-strengthening substituent on the carbon. ...
Some pyridoxal analogs and their transamination with amino acids
... of the roles of vitamin Bg 'coenzymes in enzymic reactions has developed from several different types of experimental ap proaches. The approaches which will be emphasized in this review include investigations on nonenzymic model systems and studies of enzyme systems reacting with antagonists (carbo ...
... of the roles of vitamin Bg 'coenzymes in enzymic reactions has developed from several different types of experimental ap proaches. The approaches which will be emphasized in this review include investigations on nonenzymic model systems and studies of enzyme systems reacting with antagonists (carbo ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.