9. Wakil, S. J., Green, DE, Mii, S., and Mahler, HR (1954) Studies on
... extended his studies of fatty acid biosynthesis to the Eschericia coli and identified the individual enzymes that constitute the pathway for fatty acid synthesis in bacteria which offering additional weights into the construction of fatty acid chains in animals. Using the bacterial system, he helped ...
... extended his studies of fatty acid biosynthesis to the Eschericia coli and identified the individual enzymes that constitute the pathway for fatty acid synthesis in bacteria which offering additional weights into the construction of fatty acid chains in animals. Using the bacterial system, he helped ...
Kinetics of Peroxynitrite Reaction with Amino Acids and Human
... plots of the rate constant with the sulfhydryl versus pH were bell-shaped. The kinetics of peroxynitrite reaction with all free L-amino acids were determined under pseudo-first order conditions. The most reactive amino acids were cysteine, methionine, and tryptophan. Histidine, leucine, and phenylal ...
... plots of the rate constant with the sulfhydryl versus pH were bell-shaped. The kinetics of peroxynitrite reaction with all free L-amino acids were determined under pseudo-first order conditions. The most reactive amino acids were cysteine, methionine, and tryptophan. Histidine, leucine, and phenylal ...
Vanillin formation from ferulic acid in Vanilla planifolia is catalysed
... conclude that VpVAN is catalysing vanillin and vanillin glucoside formation from ferulic acid and ferulic acid glucoside in a coupled non-oxidative hydratase/lyase reaction. A general amino-acid sequence identity search using GenBank showed that the VpVAN protein exhibits high sequence identity to c ...
... conclude that VpVAN is catalysing vanillin and vanillin glucoside formation from ferulic acid and ferulic acid glucoside in a coupled non-oxidative hydratase/lyase reaction. A general amino-acid sequence identity search using GenBank showed that the VpVAN protein exhibits high sequence identity to c ...
Organization and Integration of Large
... 1999, Oshry, 2007]. Thus, probably the greatest challenge within systems biology is the understanding and computational simulation of a complete organism. Despite the technological advances on the field of experimental methods and computational analysis tools, understanding of a multicellular organi ...
... 1999, Oshry, 2007]. Thus, probably the greatest challenge within systems biology is the understanding and computational simulation of a complete organism. Despite the technological advances on the field of experimental methods and computational analysis tools, understanding of a multicellular organi ...
Novel Allergen Structures with Tandem Amino Acid Repeats
... 7/9 (78%), and 8/9 (89%), respectively. Four sera from patients allergic to Bla g 2, but not to Bla g 1, and two sera from nonallergic patients were used as controls and gave negative results. Monoclonal antibodies against Bla g 1 (10A6), Bla g 2 (8F4), and Per a 1 (Per a 1– 03) were also tested; 10 ...
... 7/9 (78%), and 8/9 (89%), respectively. Four sera from patients allergic to Bla g 2, but not to Bla g 1, and two sera from nonallergic patients were used as controls and gave negative results. Monoclonal antibodies against Bla g 1 (10A6), Bla g 2 (8F4), and Per a 1 (Per a 1– 03) were also tested; 10 ...
Organization and Integration of Large-scale Datasets for
... 1999, Oshry, 2007]. Thus, probably the greatest challenge within systems biology is the understanding and computational simulation of a complete organism. Despite the technological advances on the field of experimental methods and computational analysis tools, understanding of a multicellular organi ...
... 1999, Oshry, 2007]. Thus, probably the greatest challenge within systems biology is the understanding and computational simulation of a complete organism. Despite the technological advances on the field of experimental methods and computational analysis tools, understanding of a multicellular organi ...
Title Biotin Biosynthesis in Microorganisms (Commemoration Issue
... 5.5 x 10-4M, 1.5X 10-3M and 1.5X10-3M, respectively. Opimum temperature was 32°C and optimum pH was 7.0. The pimelyl-CoA formed by this reaction was converted to its hydroxamate, and it was identical with the hydroxamate of synthetic pimelyl-CoA on paper chromatography. Metal chelating agents like e ...
... 5.5 x 10-4M, 1.5X 10-3M and 1.5X10-3M, respectively. Opimum temperature was 32°C and optimum pH was 7.0. The pimelyl-CoA formed by this reaction was converted to its hydroxamate, and it was identical with the hydroxamate of synthetic pimelyl-CoA on paper chromatography. Metal chelating agents like e ...
Amino Acid Catabolism: C
... by hydrolysis catalyzed by Asparaginase. This yields aspartate, which can be converted to oxaloacetate, e.g., by transamination. ...
... by hydrolysis catalyzed by Asparaginase. This yields aspartate, which can be converted to oxaloacetate, e.g., by transamination. ...
Amino Acid Catabolism: C
... by hydrolysis catalyzed by Asparaginase. This yields aspartate, which can be converted to oxaloacetate, e.g., by transamination. ...
... by hydrolysis catalyzed by Asparaginase. This yields aspartate, which can be converted to oxaloacetate, e.g., by transamination. ...
Propionic Acid Degradation by Syntrophic Bacteria During
... to maintain continuous and efficient biogas production. The real reasons for this are not yet fully described and as cultivation of propionate degraders is difficult, the knowledge about syntrophic organisms capable of anaerobic oxidation of propionic acid is restricted. Research allowing better underst ...
... to maintain continuous and efficient biogas production. The real reasons for this are not yet fully described and as cultivation of propionate degraders is difficult, the knowledge about syntrophic organisms capable of anaerobic oxidation of propionic acid is restricted. Research allowing better underst ...
Temperature-dependent effects of cadmium and purine nucleotides
... temperature in the absence of additives (control) or with addition of the varying amounts of cadmium (10–250·mol·l–1 Cd as CdCl2), purine nucleotides (0.5–10·mmol·l–1 ATP, ADP and GDP), free fatty acids (0.5–5·mol·l–1 of oleic and linoleic acid) or 2% fatty acid free bovine serum albumin (BSA). Af ...
... temperature in the absence of additives (control) or with addition of the varying amounts of cadmium (10–250·mol·l–1 Cd as CdCl2), purine nucleotides (0.5–10·mmol·l–1 ATP, ADP and GDP), free fatty acids (0.5–5·mol·l–1 of oleic and linoleic acid) or 2% fatty acid free bovine serum albumin (BSA). Af ...
1. dia
... There are many different resins and most of them are used for special cases and in individual laboratories. Here only the most widely applied resins will be presented. Resins are based on PS-DVB (1%) copolymer. 4-Alkoxybenzyl alcohol (Wang) resin: HO ...
... There are many different resins and most of them are used for special cases and in individual laboratories. Here only the most widely applied resins will be presented. Resins are based on PS-DVB (1%) copolymer. 4-Alkoxybenzyl alcohol (Wang) resin: HO ...
Raines, ChemRev 1998
... its integrity and solubility under drastic conditions: first, 0.25 N sulfuric acid at 5 °C, and then, pH 3.0 at 95-100 °C.84 The final step in this protocol calls for crystallization of the enzyme. The three-dimensional structure of RNase A is fully encoded by its amino acid sequence.85-89 This disc ...
... its integrity and solubility under drastic conditions: first, 0.25 N sulfuric acid at 5 °C, and then, pH 3.0 at 95-100 °C.84 The final step in this protocol calls for crystallization of the enzyme. The three-dimensional structure of RNase A is fully encoded by its amino acid sequence.85-89 This disc ...
Differential effects of nipecotic acid and w x 4,5,6,7
... 1990.. Accordingly, a reduction in the efficacy of GABAergic neurons normally results in seizures or epileptic episodes ŽHeinemann and Jones, 1990.. In recent years it has been established that compounds capable of inhibiting GABA transport and in particular astroglial uptake can act as anticonvulsi ...
... 1990.. Accordingly, a reduction in the efficacy of GABAergic neurons normally results in seizures or epileptic episodes ŽHeinemann and Jones, 1990.. In recent years it has been established that compounds capable of inhibiting GABA transport and in particular astroglial uptake can act as anticonvulsi ...
Par-1
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
NGRLW_SPODS_2.2 - National Genetics Reference Laboratories
... introduction of automation, many laboratories undertake complete redesign of primer sets for their genes of interest. This design and optimisation process can be relatively time consuming and may often be replicated for a particular gene in different laboratories. This document details a Standardise ...
... introduction of automation, many laboratories undertake complete redesign of primer sets for their genes of interest. This design and optimisation process can be relatively time consuming and may often be replicated for a particular gene in different laboratories. This document details a Standardise ...
Aalborg Universitet metabolic engineering approach
... a concept can be seen as one part of a solution as it gives the possibility of replacing oil refinery products with sustainable biomass derived substitutes. There are many types of biorefinery systems; the most described type is probably the enzymatic and microbial conversion of lignocellulose to di ...
... a concept can be seen as one part of a solution as it gives the possibility of replacing oil refinery products with sustainable biomass derived substitutes. There are many types of biorefinery systems; the most described type is probably the enzymatic and microbial conversion of lignocellulose to di ...
Characterization of the Phosphoenolpyruvate Carboxykinase Gene
... O06084), Chlorobium limicola (SWISS-PROT Q08262) and Treponema pallidum SWISS-PROT O83159), however, these four genes were identified during sequencing projects only by sequence similarity and can therefore not be considered as being functionally analyzed. The present study describes for the first t ...
... O06084), Chlorobium limicola (SWISS-PROT Q08262) and Treponema pallidum SWISS-PROT O83159), however, these four genes were identified during sequencing projects only by sequence similarity and can therefore not be considered as being functionally analyzed. The present study describes for the first t ...
Identification and expression of GH
... 0377BP (Choi et al., 2004), which are classed as GH-8 chitosanases. Based on this result, two further primers (BTMO-F and BTMO-R) were designed and PCR reactions were performed with the chromosomal DNAs of all 29 B. thuringiensis strains. As summarized in Table 1, PCR products were obtained from eac ...
... 0377BP (Choi et al., 2004), which are classed as GH-8 chitosanases. Based on this result, two further primers (BTMO-F and BTMO-R) were designed and PCR reactions were performed with the chromosomal DNAs of all 29 B. thuringiensis strains. As summarized in Table 1, PCR products were obtained from eac ...
Metabolism of bile acids
... addition, hepatocytes contain other transport proteins that may import bile salts, including the organic anion transporting polypeptide-C (OATP-C, SLC21A6), OATP-A (SLC21A3), OATP-B (SLC21A9) and OATP8 (SLC21A8) [25]. These transporters have a broad substrate specificity, they are bidirectional and ...
... addition, hepatocytes contain other transport proteins that may import bile salts, including the organic anion transporting polypeptide-C (OATP-C, SLC21A6), OATP-A (SLC21A3), OATP-B (SLC21A9) and OATP8 (SLC21A8) [25]. These transporters have a broad substrate specificity, they are bidirectional and ...
Lactobacillus paracasei
... rhamnosus (Hansen 1968; ATCC 7469T) and Lactobacillus casei subsp. alactosus (Mills and Lessel. (1973; ATCC 27216T). However, Johnson (1973) and Dellaglio et al. (1975) found high DNA:DNA homology between strains designated L. casei subsp. casei and L. casei subsp. alactosus, and in Bergey‟s Manual ...
... rhamnosus (Hansen 1968; ATCC 7469T) and Lactobacillus casei subsp. alactosus (Mills and Lessel. (1973; ATCC 27216T). However, Johnson (1973) and Dellaglio et al. (1975) found high DNA:DNA homology between strains designated L. casei subsp. casei and L. casei subsp. alactosus, and in Bergey‟s Manual ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.