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Snorks Lab File
Snorks Lab File

... DNA TO PROTEINS Demonstrate how the genetic information in DNA molecules provides instructions for assembling protein molecules and that this is virtually the same mechanism for all life forms. ...
XTalkDB: a database of signaling pathway crosstalk
XTalkDB: a database of signaling pathway crosstalk

... each abstract. Otherwise, we selected paper titles that we judged were likely to report crosstalk. vi) Note that some publications may support crosstalk from pathway A to pathway B and other papers in the other direction. It is also possible for a publication to support crosstalk in both directions. ...
Introduction to Genetics
Introduction to Genetics

... preceding biology course. We begin by considering the importance of genetics to each of us, to society at large, and to students of biology. We then turn to the history of genetics, how the field as a whole developed. The final part of the chapter reviews some fundamental terms and principles of gen ...
BT314 Virology
BT314 Virology

... preceding biology course. We begin by considering the importance of genetics to each of us, to society at large, and to students of biology. We then turn to the history of genetics, how the field as a whole developed. The final part of the chapter reviews some fundamental terms and principles of gen ...
Regulatory Motifs in DNA Sequences
Regulatory Motifs in DNA Sequences

Muscle alanine synthesis and hepatic gluconeogenesis
Muscle alanine synthesis and hepatic gluconeogenesis

Biosynthesis of theanine (g-ethylamino-L
Biosynthesis of theanine (g-ethylamino-L

... examined (Fig. 5). When whole seedlings were used, incorporation of 15N into theanine in roots increased essentially linearly with incubation time. 15N was also incorporated into theanine in shoots, but at a lower rate than in roots. Incorporation into cotyledons was negligible (Fig. 5A). Rates of i ...
To remember Sir Hans Krebs: Nobelist, Friend, and Adviser
To remember Sir Hans Krebs: Nobelist, Friend, and Adviser

... illustration of the Krebs cycle. As Krebs explained in his Nobel prize lecture, “Of major significance was another new observation. Citrate was not only broken down at a rapid rate but was also readily formed in muscle and in other tissues. ”g The Krebs cycle, according to E. Hammarsten, Karohnska I ...
using polymerase chain reaction for detection of enterotoxigenic
using polymerase chain reaction for detection of enterotoxigenic

... Key words: Enterotoxigenic Vibrio cholerae, enterotoxin, cholera toxin, polymerase chain reaction (PCR) INTRODUCTION Cholera has been one of the most feared diseases for human. It was recognized that two biotypes were agglutinated by a single antiserum designated O1 in the 1930s. Other strains of Vi ...
universidade estadual de maringá metabolic alterations caused by
universidade estadual de maringá metabolic alterations caused by

... predominantly its activity as an uncoupler. The observations corroborating uncoupling, which have also been traditionally reported for other uncouplers of oxidative phosphorylation, are: a) stimulation of oxygen consumption at low concentrations; b) diminution of the ATP content combined with diminu ...
Amino Acids in Animal Nutrition
Amino Acids in Animal Nutrition

... to produce the quantity of meat, milk, fish and eggs demanded by European consumers. The availability of amino acids has allowed feeds to be produced using smaller quantities of protein rich raw materials allowing these limited scarce resources to be used more sparingly. Starting in the 1950’s with ...
Taurine Deficiency in Cats
Taurine Deficiency in Cats

... Figure 1. Chemical structure of taurine ...
Engineering of Aromatic Amino Acid Metabolism in
Engineering of Aromatic Amino Acid Metabolism in

Presence of multiple group I introns closely 23S rRNAs of lichen-forming
Presence of multiple group I introns closely 23S rRNAs of lichen-forming

... [17], characteristic of the HE involved in intron mobility. The LAGLIDADG family of HE comprises more than 200 proteins and is the most diverse of the HE families since its members are present in the genomes of plant and algal chloroplasts, fungal and protozoan mitochondria, bacteria, and archaea [3 ...
Wolbachia John H. Werren and Jeremy D. Bartos
Wolbachia John H. Werren and Jeremy D. Bartos

... nearly identical to each other, with only a single base pair difference between two subsets. N. giraulti sequences were also nearly identical, with two sequences sharing a single base pair difference and one sequence containing a single unique difference. N. giraulti and N. longicornis differed from ...
Cloning, Sequencing, and Characterization of Luciola italica
Cloning, Sequencing, and Characterization of Luciola italica

... collected in the countryside of Bologna, Italy, flash frozen in liquid nitrogen and total RNA was extracted from the firefly lanterns. The L. italica luciferase cDNA was successfully cloned by RT-PCR using a genespecific primer set based on the DNA sequence of the Eastern European Luciola mingrelica ...
Notes for Chaper 4 of the Jones/Pevzer book
Notes for Chaper 4 of the Jones/Pevzer book

... An Introduction to Bioinformatics Algorithms ...
Schlage Open Options AD-300 AD-400 Solution Sheet
Schlage Open Options AD-300 AD-400 Solution Sheet

OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed

... and hence the fragments are size fractionated • The resolution of separation depends on the type and concentration of gel and the length of the run: ‚mini‘ gels are fairly low resolution and typically used for separating fragments that differ by >100 bp • By adding a fluorescent marker that is incor ...
4. characterisation of novel proteins
4. characterisation of novel proteins

... cassette encoding the two desaturase enzymes at a single genetic locus. The promoters and leader sequences used in the gene constructs are soybean-derived elements, known to be spatially and temporally active only in the developing seed. Data generated over three generations confirmed stability of t ...
Staphylococcus haemolyticus lipase
Staphylococcus haemolyticus lipase

Flavin coenzymes
Flavin coenzymes

... Mo – e.g. nitrogenase (固氮酶) Se – e.g. glutathion peroxidase (谷胱苷肽过氧化物酶) Mn, V, etc. ...
PATRICK PROBLEMS 2 Key
PATRICK PROBLEMS 2 Key

... Note the presence of the tetrahedral centre bearing the alcohol group - also present in the intermediate. Note also that there is an extra methylene group between this tetrahedral centre and proline. This is necessary if the inhibitor is to be stable to the enzyme. Further details regarding the desi ...
Metabolism and biochemical characteristics of yogurt bacteria
Metabolism and biochemical characteristics of yogurt bacteria

... 2 strains of subspecies salivarius of S salivarius. This cross-hybridization suggests that both species share common DNA sequences, but more data are needed to support a possible close relationship between these 2 streptococci. In addition, the probe easily detects RFLP (restriction fragment length ...
Synthesis and Pharmacological Evaluation of
Synthesis and Pharmacological Evaluation of

... Cancer, a disease of worldwide importance, is a group of diseases characterized by uncontrolled growth and spread of abnormal cells. It is now generally accepted that a neoplastic transformation is related to genes alteration or oncogene activation, allowing progress in the development of new treatm ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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