A12_ScienceAndFiction - Collegiate Quiz Bowl Packet Archive
... Commemoration festival* that is happening in the city of Christminster. At this point, the protagonist and his wife, Arabella, have been married twice, although the love of his life will always be Sue Bridehead. However, the two of them decided to break it off when the titular protagonist’s eldest s ...
... Commemoration festival* that is happening in the city of Christminster. At this point, the protagonist and his wife, Arabella, have been married twice, although the love of his life will always be Sue Bridehead. However, the two of them decided to break it off when the titular protagonist’s eldest s ...
Nucleotide sequence of the Tn10 encoded tetracycline resistance
... The finding of a complex, overlapping promotor system for the tetracycline resistance gene carried on Tn10 and described in figure 3 is in agreement with previously published data [2]. A similar arrangement of overlapping promotors has been described for the tet proraotor of pBR322 [25]. The particu ...
... The finding of a complex, overlapping promotor system for the tetracycline resistance gene carried on Tn10 and described in figure 3 is in agreement with previously published data [2]. A similar arrangement of overlapping promotors has been described for the tet proraotor of pBR322 [25]. The particu ...
Thermodynamic and Biophysical Characterization of Cytochrome P450
... catalyze the oxidative cleavage of long-chain fatty acyl CoA substrates (involving multiple successive P450-mediated reactions) to produce pimeloyl CoA, a known intermediate in the biotin synthesis pathways of other bacteria (17, 18). In these experiments, enzyme activity was reconstituted using a r ...
... catalyze the oxidative cleavage of long-chain fatty acyl CoA substrates (involving multiple successive P450-mediated reactions) to produce pimeloyl CoA, a known intermediate in the biotin synthesis pathways of other bacteria (17, 18). In these experiments, enzyme activity was reconstituted using a r ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... reactions are singleplex. Please note that the “multiplex” in the GMO kit is slightly different than the “multiplexing” described above. In the GMO Investigator kit, primer pairs are mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I ...
... reactions are singleplex. Please note that the “multiplex” in the GMO kit is slightly different than the “multiplexing” described above. In the GMO Investigator kit, primer pairs are mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I ...
CHAPTER 16 ACID-BASE EQUILIBRIA AND SOLUBILITY
... Could you have predicted whether the pH should have increased or decreased after the addition of the sodium acetate to the pure 0.40 M acetic acid in part (a)? An alternate way to work part (b) of this problem is to use the Henderson-Hasselbalch equation. pH = pKa + log ...
... Could you have predicted whether the pH should have increased or decreased after the addition of the sodium acetate to the pure 0.40 M acetic acid in part (a)? An alternate way to work part (b) of this problem is to use the Henderson-Hasselbalch equation. pH = pKa + log ...
The metabolism of glucose by Pseudomonas aeruginosa
... Bacteria, because of the ease with which they may be grown, offer particular promise for the study of these newer metabolic pathways, Hhe research problems in this field involve mainly a study of the breakdown of hexoses by an oxidative mechanism either with or without the involvement of phosphate. ...
... Bacteria, because of the ease with which they may be grown, offer particular promise for the study of these newer metabolic pathways, Hhe research problems in this field involve mainly a study of the breakdown of hexoses by an oxidative mechanism either with or without the involvement of phosphate. ...
L19 Alkaloids MedicinalPlants
... primarily there are general alkaloid test and specific alkaloid test; -general test : can be applied to all classes of alkaloids. -specific test: for certain pure compound, or certain class or related compounds, for example; marqui test for caffeine, while taloquine for quinine. We cannot obtain pos ...
... primarily there are general alkaloid test and specific alkaloid test; -general test : can be applied to all classes of alkaloids. -specific test: for certain pure compound, or certain class or related compounds, for example; marqui test for caffeine, while taloquine for quinine. We cannot obtain pos ...
Kinetic studies of human tyrosyl
... Wild-type and mutant human TDP enzymes were expressed in E. coli TOP10 cells bearing pBAD/Thio-huTDP, pBAD/Thio-huTDPND39 and pBAD/Thio-huTDPND174, respectively. After induction with 0.02% arabinose for 2 h, E. coli cells were pelleted and broken in lysis buffer by sonication. Cell lysate was separa ...
... Wild-type and mutant human TDP enzymes were expressed in E. coli TOP10 cells bearing pBAD/Thio-huTDP, pBAD/Thio-huTDPND39 and pBAD/Thio-huTDPND174, respectively. After induction with 0.02% arabinose for 2 h, E. coli cells were pelleted and broken in lysis buffer by sonication. Cell lysate was separa ...
Uptake of organic nitrogen by plants
... by the prominent role of inorganic N in many arable soils and the dependence of many crop plants on this N source. It was also, naturally, motivated by the abundance of inorganic N fertilizers for agricultural use. After the widely discussed human perturbation of the global carbon (C) cycle, anthrop ...
... by the prominent role of inorganic N in many arable soils and the dependence of many crop plants on this N source. It was also, naturally, motivated by the abundance of inorganic N fertilizers for agricultural use. After the widely discussed human perturbation of the global carbon (C) cycle, anthrop ...
as a PDF - CiteSeerX
... Chromosomal recombination has both beneficial and deleterious consequences. During meiosis, recombination is generally considered to be essential to the orderly distribution of chromosomes. In mitotically growing cells of lower organisms, recombination provides for efficient repair of DNA damage, pa ...
... Chromosomal recombination has both beneficial and deleterious consequences. During meiosis, recombination is generally considered to be essential to the orderly distribution of chromosomes. In mitotically growing cells of lower organisms, recombination provides for efficient repair of DNA damage, pa ...
niculescu m - Revista de Chimie
... Development of concepts for collagen-based biomaterials began many years ago and new elements are constantly revealed [5, 6]. At the moment there is a rich palette of materials based on protein extracted from collagen and collagen matrix materials, mainly used in medical, pharmaceutical and cosmetic ...
... Development of concepts for collagen-based biomaterials began many years ago and new elements are constantly revealed [5, 6]. At the moment there is a rich palette of materials based on protein extracted from collagen and collagen matrix materials, mainly used in medical, pharmaceutical and cosmetic ...
Human RIF1 and protein phosphatase 1 stimulate DNA replication
... replicative helicase core complex MCM2-7 is known to affect DNA synthesis only mildly, as each cell cycle an excess of MCM2-7 becomes loaded on DNA, which serves as dormant origins. These origins have important functions in case of replication fork blockage and allow in this case reestablishment of ...
... replicative helicase core complex MCM2-7 is known to affect DNA synthesis only mildly, as each cell cycle an excess of MCM2-7 becomes loaded on DNA, which serves as dormant origins. These origins have important functions in case of replication fork blockage and allow in this case reestablishment of ...
Landick R, Yanofsky C. 1987. Transcription
... segment of the leader region. While the polymerase pauses in the leader region, the opportunity is provided for loading of a ribosome at the leader peptide ribosome-binding site of the leader transcript. After a ribosome loads and begins translation, it approaches the paused polymerase. As it nears ...
... segment of the leader region. While the polymerase pauses in the leader region, the opportunity is provided for loading of a ribosome at the leader peptide ribosome-binding site of the leader transcript. After a ribosome loads and begins translation, it approaches the paused polymerase. As it nears ...
rec-mediated recombinational hot spot activity in bacteriophage
... each cross is density-labeled and carries a marker in the A gene furthest left on the vegetative map (gene A ) , while the other parent is unlabeled and carries a marker in the furthest right A gene ( R ) .An A+R+ recombinant from this cross can conceivably be of essentially any density from nearly ...
... each cross is density-labeled and carries a marker in the A gene furthest left on the vegetative map (gene A ) , while the other parent is unlabeled and carries a marker in the furthest right A gene ( R ) .An A+R+ recombinant from this cross can conceivably be of essentially any density from nearly ...
AMINO ACID OXIDATION AND THE PRODUCTION OF UREA
... 3. During starvation or in uncontrolled diabetes mellitus, when carbohydrates are either unavailable or not properly utilized, cellular proteins are used as fuel. Under all these metabolic conditions, amino acids lose their amino groups to form -keto acids, the “carbon skeletons” of amino acids. Th ...
... 3. During starvation or in uncontrolled diabetes mellitus, when carbohydrates are either unavailable or not properly utilized, cellular proteins are used as fuel. Under all these metabolic conditions, amino acids lose their amino groups to form -keto acids, the “carbon skeletons” of amino acids. Th ...
Introduction to Carbohydrates
... length.] The carbons in a fatty acid can also be counted beginning at the ω (or methyl-terminal) end of the chain. • Arachidonic acid is referred to as an ω-6 fatty acid because the closest double bond to the ω end begins six carbons from that end. • Another ω-6 fatty acid is the essential linoleic ...
... length.] The carbons in a fatty acid can also be counted beginning at the ω (or methyl-terminal) end of the chain. • Arachidonic acid is referred to as an ω-6 fatty acid because the closest double bond to the ω end begins six carbons from that end. • Another ω-6 fatty acid is the essential linoleic ...
aminoacids
... Its hydrolysis yields urea Takes part in urea cycle Contributes in the formation of creatine An essential aminoacid Does not allow formation of α-helix ...
... Its hydrolysis yields urea Takes part in urea cycle Contributes in the formation of creatine An essential aminoacid Does not allow formation of α-helix ...
Microbial Genetics, Mutation and Repair Answer the following
... 7. Genetic information transferred from one bacteria to another using a pilus is known as _____________. 8. F+ cells contain a fertility _____________. 9. An ____________ plasmid contains both the genetic information needed for a sex pilus as well as parts of the bacterial chromosome. 10. Plasmids t ...
... 7. Genetic information transferred from one bacteria to another using a pilus is known as _____________. 8. F+ cells contain a fertility _____________. 9. An ____________ plasmid contains both the genetic information needed for a sex pilus as well as parts of the bacterial chromosome. 10. Plasmids t ...
Production of long chain alcohols and alkanes upon coexpression of
... activity. We next evaluated 8 ADOs from cyanobacteria and discovered a homolog from Cyanothece sp. pcc 7425 that when expressed with AAR results in a B5 higher accumulation of long chain alcohols and alkanes in E. coli BL21(DE3) for a combined titer of B10 mg L 1 pentadecane, heptadecane, tetradeca ...
... activity. We next evaluated 8 ADOs from cyanobacteria and discovered a homolog from Cyanothece sp. pcc 7425 that when expressed with AAR results in a B5 higher accumulation of long chain alcohols and alkanes in E. coli BL21(DE3) for a combined titer of B10 mg L 1 pentadecane, heptadecane, tetradeca ...
Branched-chain 2-oxoacids transamination increases
... stimulated by high glucose [1-4]. Early studies showed that BCKA were transaminated in a reaction probably catalyzed by a specific branched-chain 2-oxoacid aminotransferase (BCAT) activity [5-8]. Moreover, they can be oxidised to CO2 an acetoacetate [5,9,10] and it has been shown that oxo-4-methylpe ...
... stimulated by high glucose [1-4]. Early studies showed that BCKA were transaminated in a reaction probably catalyzed by a specific branched-chain 2-oxoacid aminotransferase (BCAT) activity [5-8]. Moreover, they can be oxidised to CO2 an acetoacetate [5,9,10] and it has been shown that oxo-4-methylpe ...
CH 2 -CH 2 -CH 2 -CH 2
... •8 FADH2 = 16 ATP (by oxidative phosphorylation) •8 GTP = 8 ATP •7 NADH generated by beta oxidation itself = 21 ATP (by oxidative phosphorylation) •7 FADH2 generated by beta oxidation itself = 14 ATP (by oxidative phosphorylation) Total number of ATP from 1 molecule of palmitate = 72 + 16 + 8 + 21 + ...
... •8 FADH2 = 16 ATP (by oxidative phosphorylation) •8 GTP = 8 ATP •7 NADH generated by beta oxidation itself = 21 ATP (by oxidative phosphorylation) •7 FADH2 generated by beta oxidation itself = 14 ATP (by oxidative phosphorylation) Total number of ATP from 1 molecule of palmitate = 72 + 16 + 8 + 21 + ...
Endonucleolytic processing of CCAless tRNA precursors by RNase
... directly responsible for tRNA maturation in B.subtilis and whether the CCA motif was indeed inhibitory for cleavage, we cloned the gene in-phase with the coding sequence for a C-terminal histidine tag and puri®ed YqjK from overexpressing E.coli cells (Figure 5A). The activity of the YqjK protein was ...
... directly responsible for tRNA maturation in B.subtilis and whether the CCA motif was indeed inhibitory for cleavage, we cloned the gene in-phase with the coding sequence for a C-terminal histidine tag and puri®ed YqjK from overexpressing E.coli cells (Figure 5A). The activity of the YqjK protein was ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.