Effect of Alanine-293 Replacement on the Activity, ATP Binding, and
... aminoacylation activity of LeuRS than any of the other substitutions A293D, A293R, A293G, A293I, A293Y and A293F. 293A is only involved in the binding of ATP, and all amino acid substitutions above caused stronger binding of ATP. Moreover, the negative charge at this site, induced by mutation A293D, ...
... aminoacylation activity of LeuRS than any of the other substitutions A293D, A293R, A293G, A293I, A293Y and A293F. 293A is only involved in the binding of ATP, and all amino acid substitutions above caused stronger binding of ATP. Moreover, the negative charge at this site, induced by mutation A293D, ...
The Protein Cevalently Linked to the 5'... of Poliovirus RNA by Victor Robert Ambros
... be recovered from poliovirus RNA chains varying in length from 7,50C nucleotides (full-sized RNA) to about 500 nucleotides. ...
... be recovered from poliovirus RNA chains varying in length from 7,50C nucleotides (full-sized RNA) to about 500 nucleotides. ...
Environmental enteric dysfunction is associated with carnitine
... aliquot was analyzed using acidic, positive ion-optimized conditions. Another aliquot was analyzed using basic, negative ion-optimized conditions. Two independent injections were done using separate dedicated columns (Waters UPLC BEH C18–2.1 × 100 mm, 1.7 μm). Extracts reconstituted in acidic condit ...
... aliquot was analyzed using acidic, positive ion-optimized conditions. Another aliquot was analyzed using basic, negative ion-optimized conditions. Two independent injections were done using separate dedicated columns (Waters UPLC BEH C18–2.1 × 100 mm, 1.7 μm). Extracts reconstituted in acidic condit ...
Heredity & Human Affairs
... • What is the probability associated with this chi-square? • Should you accept or reject your hypothesis of a 1:1 ratio? ...
... • What is the probability associated with this chi-square? • Should you accept or reject your hypothesis of a 1:1 ratio? ...
DNA Methylation Analysis
... multiplexed genotyping of bisulfite-converted genomic DNA. First, genomic DNA is treated with a bisulfite conversion kit. The result is DNA that contains unconverted cytosines (if they were methylated) or uracils (derived from unmethylated cytosine). This differentially converted DNA is interrogated ...
... multiplexed genotyping of bisulfite-converted genomic DNA. First, genomic DNA is treated with a bisulfite conversion kit. The result is DNA that contains unconverted cytosines (if they were methylated) or uracils (derived from unmethylated cytosine). This differentially converted DNA is interrogated ...
Lactate Acid Fermentation of Acetic Acid in the Butyric Spec. and the
... bid, considerable gas was given off, and the pH rose to 8.2 to 8.4. After 72 hours a determination of lactic acid by the method of Friedemann and Graeser (1933) showed that the lactate was completely decomposed. Several soils of different types were used as inocula for enrichment cultures of this ty ...
... bid, considerable gas was given off, and the pH rose to 8.2 to 8.4. After 72 hours a determination of lactic acid by the method of Friedemann and Graeser (1933) showed that the lactate was completely decomposed. Several soils of different types were used as inocula for enrichment cultures of this ty ...
Rh phenotype prediction by DNA typing and its
... the most prevalent RHD-negative allele cannot yet be detected specifically. For the time being, we prefer therefore the term DNA typing rather than genotyping, when an RhD phenotype prediction by molecular techniques is attempted. We have summarized the molecular genetics of the RH genes according t ...
... the most prevalent RHD-negative allele cannot yet be detected specifically. For the time being, we prefer therefore the term DNA typing rather than genotyping, when an RhD phenotype prediction by molecular techniques is attempted. We have summarized the molecular genetics of the RH genes according t ...
abnormalities of chromosome structure
... MUTAGENS: agents that are known to increase the frequency of mutations. Radiation from x-rays or nuclear fallout forms electrically charged ions that produce chemical reactions which change DNA bases. A variety of chemicals can induce mutations because they are chemically similar to the DNA ba ...
... MUTAGENS: agents that are known to increase the frequency of mutations. Radiation from x-rays or nuclear fallout forms electrically charged ions that produce chemical reactions which change DNA bases. A variety of chemicals can induce mutations because they are chemically similar to the DNA ba ...
The DNA Binding Properties of Saccharomyces cerevisiae Rad51
... stoichiometric excess of protein. Without nucleotide cofactor, Rad51 protein binds both ssDNA and dsDNA but only at pH values lower than 6.8; in this case, the apparent binding stoichiometry covers the range of 1 protein monomer per 6 –9 nucleotides or base pairs. Therefore, Rad51 protein displays t ...
... stoichiometric excess of protein. Without nucleotide cofactor, Rad51 protein binds both ssDNA and dsDNA but only at pH values lower than 6.8; in this case, the apparent binding stoichiometry covers the range of 1 protein monomer per 6 –9 nucleotides or base pairs. Therefore, Rad51 protein displays t ...
Evolutionary aspects of recombination in RNA viruses
... genomes of accumulated deleterious changes (Muller, 1964) and to create or spread beneficial combinations of mutations in an efficient manner (Fisher, 1930 ; Muller, 1932), two processes which are not available to clonal organisms. Two distinct but not mutually exclusive types of genetic exchange op ...
... genomes of accumulated deleterious changes (Muller, 1964) and to create or spread beneficial combinations of mutations in an efficient manner (Fisher, 1930 ; Muller, 1932), two processes which are not available to clonal organisms. Two distinct but not mutually exclusive types of genetic exchange op ...
on the importance of cooperativity effects - RSC Publishing
... contact pair is Glu–A, which represents 32.5% of all the contact pairs. From the results gathered in ESI Table 4† it can be deduced that A (92.8%), G (65.2%), and U (71.1%) interact preferentially with Glu. If we only consider surface (inter-chain) interactions by removing Cl and SO42, all these p ...
... contact pair is Glu–A, which represents 32.5% of all the contact pairs. From the results gathered in ESI Table 4† it can be deduced that A (92.8%), G (65.2%), and U (71.1%) interact preferentially with Glu. If we only consider surface (inter-chain) interactions by removing Cl and SO42, all these p ...
Lecture 1 - "Hudel" Luecke
... Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes that compose ...
... Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes that compose ...
Connection of Propionyl-CoA Metabolism to Polyketide
... known about the cellular parameters controlling polyketide biosynthesis in fungi. We recently presented data suggesting that perturbations in the acyl-CoA pool can impair polyketide biosynthesis in A. nidulans. We found that blockage of propionate metabolism by mutation in the mcsA gene (encoding me ...
... known about the cellular parameters controlling polyketide biosynthesis in fungi. We recently presented data suggesting that perturbations in the acyl-CoA pool can impair polyketide biosynthesis in A. nidulans. We found that blockage of propionate metabolism by mutation in the mcsA gene (encoding me ...
CP1 Domain in Escherichia coli Leucyl
... Cloning and Expression of the Gene Encoding CP1Leu. The DNA fragment encoding CP1 of LeuRS (from Thr129 to Gly389) and flanked by NcoI and HindIII restriction sites was generated by PCR and inserted into the corresponding sites of pMFT7H6 to yield CP1Leu with an N-terminal His6 tag. The sequence of ...
... Cloning and Expression of the Gene Encoding CP1Leu. The DNA fragment encoding CP1 of LeuRS (from Thr129 to Gly389) and flanked by NcoI and HindIII restriction sites was generated by PCR and inserted into the corresponding sites of pMFT7H6 to yield CP1Leu with an N-terminal His6 tag. The sequence of ...
Deep Insight Section The vagaries of non-traditional mendelian Aa = aa !
... Aneuploid mode and mood of chromosomes: The results for chromosome 1 UPD's emphasize the major role of maternal meioses errors causing gamete nullisomy and disomy in cases of either maternal or parental UPD1 (8 cases). Paternal meioses errors also account for 4 cases. The perfect culprit for Non-Tra ...
... Aneuploid mode and mood of chromosomes: The results for chromosome 1 UPD's emphasize the major role of maternal meioses errors causing gamete nullisomy and disomy in cases of either maternal or parental UPD1 (8 cases). Paternal meioses errors also account for 4 cases. The perfect culprit for Non-Tra ...
Archive Microbiology
... destruction (Wagner et al., 1956). Also in this case the obtained extracts were evaporated three times in order to reduce the acid concentration. This procedure gave recoveries of some 40--50~, which agrees with the observation of Wagner et al. (1956). Before use in the Warburg assays, the obtained ...
... destruction (Wagner et al., 1956). Also in this case the obtained extracts were evaporated three times in order to reduce the acid concentration. This procedure gave recoveries of some 40--50~, which agrees with the observation of Wagner et al. (1956). Before use in the Warburg assays, the obtained ...
1 Professor D.Sci. Judit Kosáry Nutritional biochemistry of the
... after identification they got chemical names and short ‘accepted’ names, as well. The basis of the trivial system was on one hand the polar-apolar properties of the vitamin (water-soluble and fatsoluble) and the other hand the order (sequence) of isolation according to ABC. For example at first the ...
... after identification they got chemical names and short ‘accepted’ names, as well. The basis of the trivial system was on one hand the polar-apolar properties of the vitamin (water-soluble and fatsoluble) and the other hand the order (sequence) of isolation according to ABC. For example at first the ...
Saccharomyces cerevisiae Metabolic engineering of for production
... their limited ability to synthesize B-type vitamins and amino acids (Chopin, 1993). These requirements increase the cost and complicate downstream processing (Benninga, 1990; Vaidya et al., 2005). Furthermore, these prokaryotic organisms are generally unable to grow and produce organic acids at the ...
... their limited ability to synthesize B-type vitamins and amino acids (Chopin, 1993). These requirements increase the cost and complicate downstream processing (Benninga, 1990; Vaidya et al., 2005). Furthermore, these prokaryotic organisms are generally unable to grow and produce organic acids at the ...
The Enolase Superfamily: A General Strategy for Enzyme
... enzyme family and may allow identification of active site residues. The availability of increasing numbers of threedimensional structures now provides an opportunity to expand families defined on the basis of conserved catalytic properties to include enzymes that catalyze different overall reactions ...
... enzyme family and may allow identification of active site residues. The availability of increasing numbers of threedimensional structures now provides an opportunity to expand families defined on the basis of conserved catalytic properties to include enzymes that catalyze different overall reactions ...
PDF format - JOP. Journal of the Pancreas
... Context Dietary proteins and amino acids can modulate pancreatic function. Objective Our aim was to estimate the levels of plasma amino acids in chronic pancreatitis patients and study their relationship with disease characteristics as well as exocrine and endocrine insufficiency. Patients One hundr ...
... Context Dietary proteins and amino acids can modulate pancreatic function. Objective Our aim was to estimate the levels of plasma amino acids in chronic pancreatitis patients and study their relationship with disease characteristics as well as exocrine and endocrine insufficiency. Patients One hundr ...
All fatty acids are not equal: discrimination in plant membrane lipids
... storage lipids. To date, .300 naturally occurring fatty acids have been described in seed oils1,2, and it has been estimated that thousands more could be present throughout the plant kingdom. The structures of these fatty acids can vary in chain length from 8 to 24 carbons, they can have double bond ...
... storage lipids. To date, .300 naturally occurring fatty acids have been described in seed oils1,2, and it has been estimated that thousands more could be present throughout the plant kingdom. The structures of these fatty acids can vary in chain length from 8 to 24 carbons, they can have double bond ...
Molecular Plant-Microbio Interactions
... Accession number of the nucleotide sequence is AF323694. ...
... Accession number of the nucleotide sequence is AF323694. ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.