TaqMan® SNP Genotyping Assays User Guide
... User attention words Two user attention words may appear in this document. Each word implies a particular level of observation or actions as described below: Note: Provides information that may be of interest or help but is not critical to the use of the product. ...
... User attention words Two user attention words may appear in this document. Each word implies a particular level of observation or actions as described below: Note: Provides information that may be of interest or help but is not critical to the use of the product. ...
Specificity of Insertion by the Translocatable Tetracycline Resistance Element Tn10.
... different insertions within a cluster. Different insertions within a cluster usually have the same reversion frequency; however, heterogeneity in reversion frequency has been detected in at least two clusters. For most clusters, the available data are consistent with the simple possibility that all ...
... different insertions within a cluster. Different insertions within a cluster usually have the same reversion frequency; however, heterogeneity in reversion frequency has been detected in at least two clusters. For most clusters, the available data are consistent with the simple possibility that all ...
Product description P018-G1 SHOX-v03 - MRC
... SHOX gene function. - Please note that single exon deletions have a considerable chance of being a false positive result, either due to non-pathogenic copy number variants (Benito-Sanz et al. 2011a), mutations within the probe binding site (Barca-Tierno et al. 2011) or due to impurities in the DNA. ...
... SHOX gene function. - Please note that single exon deletions have a considerable chance of being a false positive result, either due to non-pathogenic copy number variants (Benito-Sanz et al. 2011a), mutations within the probe binding site (Barca-Tierno et al. 2011) or due to impurities in the DNA. ...
Designing synthetic MLPA probes - MRC
... • Without previous design experience, designing MLPA probes can be difficult. Although this protocol tries to describe MLPA probe design rules as comprehensively and clearly as possible, it is impossible to convey all our in-house probe design experience. • At MRC-Holland, probes are elaborately tes ...
... • Without previous design experience, designing MLPA probes can be difficult. Although this protocol tries to describe MLPA probe design rules as comprehensively and clearly as possible, it is impossible to convey all our in-house probe design experience. • At MRC-Holland, probes are elaborately tes ...
StanfordEncyclopedia_2016_Inheritance
... of genetic information encoded in DNA (or, in some viruses in RNA), a view often referred to as “geno-centrism”. It should be noted that the replicator concept itself does not rule out non-genetic replicators (Dawkins 1976, see also the discussion by Sterelny, Smith, & Dickison 1996). The dualinheri ...
... of genetic information encoded in DNA (or, in some viruses in RNA), a view often referred to as “geno-centrism”. It should be noted that the replicator concept itself does not rule out non-genetic replicators (Dawkins 1976, see also the discussion by Sterelny, Smith, & Dickison 1996). The dualinheri ...
NO sensing by FNR: regulation of the Escherichia coli NO
... the ¯avohaemoglobin-encoding gene of E.coli, hmp, is upregulated by NO and RNS; this appears not to involve SoxRS (Poole et al., 1996). We have reported (MembrilloHernaÂndez et al., 1998) a mechanism of hmp gene regulation that involves interaction between S-nitrosothiols and Hcy. Intracellular Hcy ...
... the ¯avohaemoglobin-encoding gene of E.coli, hmp, is upregulated by NO and RNS; this appears not to involve SoxRS (Poole et al., 1996). We have reported (MembrilloHernaÂndez et al., 1998) a mechanism of hmp gene regulation that involves interaction between S-nitrosothiols and Hcy. Intracellular Hcy ...
New variants of the tet(M) gene in Clostridium
... Analysis of the region upstream of the tet(M) genes showed that tet(M)1, tet(M)3, tet(M)5 and tet(M)6 were preceded by a complete leader peptide sequence, whereas this region was partially deleted in the region upstream of tet(M)2 and tet(M)4 for the loss of 124 bp (data not shown). The residual tra ...
... Analysis of the region upstream of the tet(M) genes showed that tet(M)1, tet(M)3, tet(M)5 and tet(M)6 were preceded by a complete leader peptide sequence, whereas this region was partially deleted in the region upstream of tet(M)2 and tet(M)4 for the loss of 124 bp (data not shown). The residual tra ...
Genetics Practice Test
... 125. How did Douglas Prasher find the gene that make the GFP protein? 126. What are two ways in which scientists transform plant cells without using plasmids? 127. Compare the genes in Dolly and the sheep from which she was cloned. 128. Describe how a scientist might make a transgenic animal. 129. H ...
... 125. How did Douglas Prasher find the gene that make the GFP protein? 126. What are two ways in which scientists transform plant cells without using plasmids? 127. Compare the genes in Dolly and the sheep from which she was cloned. 128. Describe how a scientist might make a transgenic animal. 129. H ...
TaqMan-Based Real-Time PCR for Genotyping Common
... The region chosen as the target sequence is located in the intronic sequence 5⬘ of the junction region of the gene duplication of the HP2 allele (intron 4; Fig. 1). The HapMap and NCBI databases have not reported any single-nucleotide polymorphisms within this region. Although we could have used any ...
... The region chosen as the target sequence is located in the intronic sequence 5⬘ of the junction region of the gene duplication of the HP2 allele (intron 4; Fig. 1). The HapMap and NCBI databases have not reported any single-nucleotide polymorphisms within this region. Although we could have used any ...
(From the Department of Genetics, Carnegie Institution of
... of freezing and thawing is to make the intracellular DNA labile to DNase, without, however, causing much of it to leach out of the ceils. Freezing and thawing and formaldehyde fixation have a negligible effect on unadsorbed phage, and formaldehyde fixation alone has only a mild effect on infected ce ...
... of freezing and thawing is to make the intracellular DNA labile to DNase, without, however, causing much of it to leach out of the ceils. Freezing and thawing and formaldehyde fixation have a negligible effect on unadsorbed phage, and formaldehyde fixation alone has only a mild effect on infected ce ...
Independent functions of viral protein and nucleic
... 3. The DNA of phage adsorbed to unheated bacteria is resistant to DNase, presumably because it is protected by cell structures impervious to the enzyme. Graham and collaborators (personal communication) were the first to discover the sensitization of phage DNA to DNase by adsorption to heat-killed b ...
... 3. The DNA of phage adsorbed to unheated bacteria is resistant to DNase, presumably because it is protected by cell structures impervious to the enzyme. Graham and collaborators (personal communication) were the first to discover the sensitization of phage DNA to DNase by adsorption to heat-killed b ...
The American University in Cairo School of Science and Engineering
... variable, inhibition of the SHV activity by p-chloromercuribenzoate is substrate-related; and varies depending on the substrate used for the assay; however, studies never confirmed the validity of this hypothesis (Paterson and Bonomo, 2005). 7KH6+9ȕ-lactamase family originally was found in Klebsi ...
... variable, inhibition of the SHV activity by p-chloromercuribenzoate is substrate-related; and varies depending on the substrate used for the assay; however, studies never confirmed the validity of this hypothesis (Paterson and Bonomo, 2005). 7KH6+9ȕ-lactamase family originally was found in Klebsi ...
BIO 402 - National Open University of Nigeria
... Strasburger demonstrated that the principles of ferlization developed by Oscar Hertwig for animals held also for plants. Strasburger made reciprocal crosses between different plant species and found that the results were similar. Since the egg and sperm were unequal with respect to size and amount o ...
... Strasburger demonstrated that the principles of ferlization developed by Oscar Hertwig for animals held also for plants. Strasburger made reciprocal crosses between different plant species and found that the results were similar. Since the egg and sperm were unequal with respect to size and amount o ...
Emended description of the order Chlamydiales, proposal of
... The current taxonomic classification of Chlamydia is based on limited phenotypic, morphologic and genetic criteria. This classification does not take into account recent analysis of the ribosomal operon or recently identified obligately intracellular organisms that have a chlamydia-like developmenta ...
... The current taxonomic classification of Chlamydia is based on limited phenotypic, morphologic and genetic criteria. This classification does not take into account recent analysis of the ribosomal operon or recently identified obligately intracellular organisms that have a chlamydia-like developmenta ...
Standard PDF - Wiley Online Library
... chromosomes and DNA sequences from P. carinii from mouse, ferret, and human also differ greatly from each other. The genome of a ferret P. carinii appears to be up to 1.7 times larger than those of P. carinii from other hosts. Nearly two dozen P. carinii genes have been cloned and sequenced. The typ ...
... chromosomes and DNA sequences from P. carinii from mouse, ferret, and human also differ greatly from each other. The genome of a ferret P. carinii appears to be up to 1.7 times larger than those of P. carinii from other hosts. Nearly two dozen P. carinii genes have been cloned and sequenced. The typ ...
Differential Enzyme Targeting As an Evolutionary Adaptation to
... 1994). Species that retain both translation and transcription start sites, such as the rat (Oda, Funai, and Ichiyama 1990) and the marmoset (Purdue, Lumb, and Danpure 1992), retain the potential to target AGT to both organelles. The final intracellular destination of AGT is dependent on the expressi ...
... 1994). Species that retain both translation and transcription start sites, such as the rat (Oda, Funai, and Ichiyama 1990) and the marmoset (Purdue, Lumb, and Danpure 1992), retain the potential to target AGT to both organelles. The final intracellular destination of AGT is dependent on the expressi ...
Gene Section MLL (myeloid/lymphoid or mixed lineage leukemia) Atlas of Genetics and Cytogenetics
... Variable breakpoints on both genes. Abnormal protein ...
... Variable breakpoints on both genes. Abnormal protein ...
Male-killing i n two species of insect Wolbachia 1~ THE ROYAL
... obtained when rate heterogeneity among sites was taken into account and estimated parameter values were used. The only difference between this tree and that found through parsimony was in the Glade of five Wolbachia strains containing the A. encedon and A. bipunctata male-killing strains. Within thi ...
... obtained when rate heterogeneity among sites was taken into account and estimated parameter values were used. The only difference between this tree and that found through parsimony was in the Glade of five Wolbachia strains containing the A. encedon and A. bipunctata male-killing strains. Within thi ...
Presence of multiple group I introns closely 23S rRNAs of lichen-forming
... LAGLIDADG family of HE comprises more than 200 proteins and is the most diverse of the HE families since its members are present in the genomes of plant and algal chloroplasts, fungal and protozoan mitochondria, bacteria, and archaea [37]. One reason for the wide distribution of these proteins appea ...
... LAGLIDADG family of HE comprises more than 200 proteins and is the most diverse of the HE families since its members are present in the genomes of plant and algal chloroplasts, fungal and protozoan mitochondria, bacteria, and archaea [37]. One reason for the wide distribution of these proteins appea ...
Human mitochondrial transfer RNAs: Role of pathogenic
... conceivable due to their central role in mitochondrial protein synthesis. In comparison, a little less than half of the mitochondrial mutations affect protein coding genes, which comprises 68% of the entire mitochondrial genome.42 To achieve their goal of carrying amino acids for protein synthesis, ...
... conceivable due to their central role in mitochondrial protein synthesis. In comparison, a little less than half of the mitochondrial mutations affect protein coding genes, which comprises 68% of the entire mitochondrial genome.42 To achieve their goal of carrying amino acids for protein synthesis, ...
Selecting Degenerate Multiplex PCR Primers
... Single Nucleotide Polymorphisms (SNPs) are individual base differences in DNA sequences between individuals. It is estimated that there are roughly three million SNPs in the human genome [12]. Association studies between SNPs and various diseases, as well as differences in how individuals respond to ...
... Single Nucleotide Polymorphisms (SNPs) are individual base differences in DNA sequences between individuals. It is estimated that there are roughly three million SNPs in the human genome [12]. Association studies between SNPs and various diseases, as well as differences in how individuals respond to ...
14 Chromosomes
... Some newborn babies have an abnormal number of chromosomes in their cells. A baby may have an additional chromosome, giving a total of 47 instead of the normal 46. One additional chromosome or one missing chromosome typically has deleterious effects on development and, for most chromosomes, death oc ...
... Some newborn babies have an abnormal number of chromosomes in their cells. A baby may have an additional chromosome, giving a total of 47 instead of the normal 46. One additional chromosome or one missing chromosome typically has deleterious effects on development and, for most chromosomes, death oc ...
uncorrected page proofs
... cells. A baby may have an additional chromosome, giving a total of 47 instead of the normal 46. One additional chromosome or one missing chromosome typically has deleterious effects on development and, for most chromosomes, death occurs during early development and the pregnancy never proceeds to te ...
... cells. A baby may have an additional chromosome, giving a total of 47 instead of the normal 46. One additional chromosome or one missing chromosome typically has deleterious effects on development and, for most chromosomes, death occurs during early development and the pregnancy never proceeds to te ...
Inference of homologous recombination in bacteria using whole
... largely in private. It is currently not feasible to track individual organisms in natural conditions which makes it difficult to observe the environmental perturbations experienced by particular lineages. Genomic deoxyribonucleic acid (DNA) sequencing provides a window onto how bacteria disperse, di ...
... largely in private. It is currently not feasible to track individual organisms in natural conditions which makes it difficult to observe the environmental perturbations experienced by particular lineages. Genomic deoxyribonucleic acid (DNA) sequencing provides a window onto how bacteria disperse, di ...
International Agency for Research on Cancer (IARC)
... Glutathione S-transferase-mediated conjugation of glutathione to the 8,9-epoxide reduces DNA damage, and this mechanism is important in reducing the tumour burden in experimental animals. Animal species, such as the mouse, that are resistant to aflatoxin carcinogenesis have three to five times more ...
... Glutathione S-transferase-mediated conjugation of glutathione to the 8,9-epoxide reduces DNA damage, and this mechanism is important in reducing the tumour burden in experimental animals. Animal species, such as the mouse, that are resistant to aflatoxin carcinogenesis have three to five times more ...
Extrachromosomal DNA
Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.