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University of Groningen To dehydrate or not Khusainov, Rustem
University of Groningen To dehydrate or not Khusainov, Rustem

... threonine residues substituted by alanine in either one of the five rings and two mutants containing double point mutations, namely either in the ring A and D, or B and E, were generated (Fig. 1) . Fig. 2 shows examples of MALDI-TOF spectra, which were obtained after analysis of FPLC purified and fu ...
Mechanism of Posttranslational Regulation of Phenol
Mechanism of Posttranslational Regulation of Phenol

... reported previously (15) that many nucleotides bind tightly to PST at a Kd in the nanomolar range. However, to remain bound to PST through a series of protein purification procedures, the nucleotide might need to be irreversibly trapped inside the enzyme. As reported later for the conversion of the ...
Structure and Function of Thymosin β4
Structure and Function of Thymosin β4

... further confirmed with mutant K11P. K11P breaks the helix and renders thymosin β4 essentially inactive. This shows that an intact helix, along with the hydrophobic core, is essential for its activity. Further analysis shows that if the N-terminal helix was made just one residue longer, the placement ...
Intra- and Intergeneric Similarities of Pseudomonas and
Intra- and Intergeneric Similarities of Pseudomonas and

... marginata, Pseudomonas caryophylli, and Pseudomonas lemoignei. Each of these rRNA branches is as heterogeneous as a genus. The Pseudomonas solanacearum and Pseudomonas acidovorans rRNA branches are about as far removed from each other as they are from the genera Janthinobacterium and Derxia and the ...
The Proposed Effects of Nicotinamide Adenine Dinucleotide (NAD
The Proposed Effects of Nicotinamide Adenine Dinucleotide (NAD

... true then this could have advantageous implications in sporting performance (Figure 1). Arterial oxygen content does not decrease at exercise intensities <75% of VO2max [49]. VO2max is a measure of the ability of working muscles to oxidise metabolic substrates, with eventually a plateau in oxygen up ...
Identification and characterization of novel interaction
Identification and characterization of novel interaction

... C-terminal domain of the α subunit of the RNA polymerase C-terminal domain of the α subunit of the RNA polymerase Adenosindiphosphate Ampicillin Adenosinmomophosphate Ammoniumperoxodisulfate Adenosintriphosphate Bacillus base pair bovine serum albumin casamino acids cyclic Adenosinmomophosphate Cata ...
practice oxidative phosphorylation worksheet11
practice oxidative phosphorylation worksheet11

... A series of proteins in the inner membrane will be used to do so. Active transport utilized to move H+ against concentration gradient in the Electron Transport Chain! A series of redox reactions are employed as H+ ions are moved against a concentration gradient via active transport. Protein Complexe ...
Chapter 4: Cellular Metabolism
Chapter 4: Cellular Metabolism

... III. Control of Metabolic Reactions A. Enzyme Action (Outcome 4.5, 4.6) 1. Metabolic reactions require ________ before they proceed. (Outcome 4.5) 2. Heat energy increases _________________________________ and ______________________________________________________________ . (Outcome 4.5) 3. The coll ...
Localization and nucleotide specificity of Blastocystis succinyl‐CoA
Localization and nucleotide specificity of Blastocystis succinyl‐CoA

... E + succinyl-COA + Pi ↔ E − succinyl-PO3 + CoA ...
6.
6.

... shown that the group of non-CAS exons significantly differs from the group of CAS exons in many important parameters (discussed previously) (these differences are summarized in Table 1). This suggests that many of the apparently non-conserved splice variants in the human genome are non-functional. I ...
Yeast Supplemental Material
Yeast Supplemental Material

... fermentation. Cell division is decreased, leading to prevention of cell division if CO2 saturation conditions remain for too long during fermentation. This also leads to higher fatty acid contents in the cell membranes and high DNA and RNA contents in the nucleus. Transport proteins are disabled cau ...
Energy and Muscle Contraction
Energy and Muscle Contraction

... Assuming a person is adequately nourished, the cellular respiration will proceed at a rate that is directly proportional to oxygen delivery. If oxygen delivery increases, ATP production increases. If oxygen delivery decreases, ATP production decreases. ATP will be produced by cellular respiration is ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
Genome-wide Investigation of Cellular Functions for tRNA Nucleus

... biosynthesis, and leucine biosynthesis pathways, are primary targets of the tRNA trafficking processes. I confirmed the microarray data by both Northern and Western blot analyses. Levels of all tested target proteins involved in such amino acid biogenesis pathways are down-regulated when the tRNA n ...
Genetic Algorithms and their Application to the Artificial Evol
Genetic Algorithms and their Application to the Artificial Evol

... reshuffling of existing information But can have strong effects on phenotype STRI, University of Hertfordshire ...
Nicotinamide adenine dinucleotide homeostasis and
Nicotinamide adenine dinucleotide homeostasis and

... increased [33] on the contrary to what was observed in the guinea pig model cited above [31]. Differences in the method of NADH quantifications and the stage of HF in different animal models may account for this discrepancy. In the study of Liu M et al. [33], INa was decreased in failing cardiomyocy ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
Bacterial ribosome requires multiple L12 dimers for efficient initiation

... (L12 NTD)6 complex from T. maritima (1). It has been proposed (13) and later evidenced with FRET (18) that on the ribosome L12 dimer can exist with one hinge compact and the other extended. Although thought to be important for translation factor recruitment, physiological relevance of such conformat ...
Data mining for important amino acid residues in multiple sequence
Data mining for important amino acid residues in multiple sequence

... β-sheet which are linked by flexible regions, called loops. These folding patterns are mostly stabilized by backbone interactions like hydrogen bonds in which hydrogens are bound to highly electronegative atoms such as nitrogen and oxygen. A single protein usually contains several of these secondary ...
Gene Expression Profiles of Cold
Gene Expression Profiles of Cold

Raines, ChemRev 1998
Raines, ChemRev 1998

... its integrity and solubility under drastic conditions: first, 0.25 N sulfuric acid at 5 °C, and then, pH 3.0 at 95-100 °C.84 The final step in this protocol calls for crystallization of the enzyme. The three-dimensional structure of RNase A is fully encoded by its amino acid sequence.85-89 This disc ...
Xenopus Spinal Neurons Express Kv2 Potassium Channel
Xenopus Spinal Neurons Express Kv2 Potassium Channel

... and pXb4 occur at the third position of a codon and do not cause amino acid changes (Fig. I/l). pXb3 is 76 and 97% identical to XShab9 and XShab12, respectively, indicating that pXb3 is related to XShab12. Eight of the nine nucleotide differences between pXb3 and XShabl2 occur at the third position ...
34386 - PubAg
34386 - PubAg

... Volicitin Induction of Igl Expression Is Fast and Transient. The kinetics of Igl induction were investigated by Northern blot analysis with the bx1bx1 mutant maize line. GAP C, a gene not inf luenced by volicitin treatment (Fig. 2), was used to normalize for loading differences on the gels. The low ...
Structure of the Actin-Myosin Complex and Its Implications for
Structure of the Actin-Myosin Complex and Its Implications for

... this model is the idea that the crossbridge first binds in a weakly binding conformation and then undergoes an isomerization to a strongly binding form. The power stroke must occur within the tightly bound states in order that the energy of hydrolysis can be transduced as movement to the array of fi ...
Mouse Lefty2 and Zebrafish Antivin Are Feedback
Mouse Lefty2 and Zebrafish Antivin Are Feedback

... The murine “lefty” locus is composed of two highly conserved genes, lefty1 and lefty2, which are tightly linked on chromosome 1 (Meno et al., 1997). To examine the role of lefty2 in gastrulation and L–R asymmetry, we generated mutant mice deficient in lefty2. To inactivate lefty2, we constructed a t ...
Interaction between calmodulin and ER
Interaction between calmodulin and ER

... altered, facilitating its association with corepressors and blocking interaction with coactivators. Gene transcription is thus inhibited. ...
Characterization of the regulatory function of the 46
Characterization of the regulatory function of the 46

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