22. Recombinant DNA Technology
... 1. Heat shock: CaCl2 at 0oC then heat to 37-42oC 2. Electroporation – apply high voltage BAC – 5,000 to 400,000 bp insert ...
... 1. Heat shock: CaCl2 at 0oC then heat to 37-42oC 2. Electroporation – apply high voltage BAC – 5,000 to 400,000 bp insert ...
Microorganisms in Biotechnology
... depositing the new gene in the chromosome of that cell • The gene is then passed on to daughter cells as the cell divides ...
... depositing the new gene in the chromosome of that cell • The gene is then passed on to daughter cells as the cell divides ...
a10c Biotechnology
... 2. What is a restriction enzyme, and what does it catalyze? How do restriction enzymes differ in what they cleave? What do they "look for"? Name an example of a restriction enzyme. 3. Describe the steps of cloning (transferring a gene to bacteria for purposes of "growing" DNA or protein). What enzym ...
... 2. What is a restriction enzyme, and what does it catalyze? How do restriction enzymes differ in what they cleave? What do they "look for"? Name an example of a restriction enzyme. 3. Describe the steps of cloning (transferring a gene to bacteria for purposes of "growing" DNA or protein). What enzym ...
Genetic Engineering - Duplin County Schools
... • Continued breeding of individuals with similar characteristics • Useful in retaining a certain set of characteristics • Can produce some serious genetic defects ...
... • Continued breeding of individuals with similar characteristics • Useful in retaining a certain set of characteristics • Can produce some serious genetic defects ...
Worksheet for 4/16
... 4. PCR is a process used to clone a specific fragment of DNA. What are the 4 main components in a PCR and what are their purposes? ...
... 4. PCR is a process used to clone a specific fragment of DNA. What are the 4 main components in a PCR and what are their purposes? ...
Genetic Engineering Short Notes
... 1. Genetic engineering- remaking genes for practical purposes 2. Recombinant DNA- DNA made from two or more different organisms 3. Restriction enzyme- enzymes that recognize short specific DNA sequences and that cut the DNA there 4. Plasmid- small, circular DNA molecules that can replicate independa ...
... 1. Genetic engineering- remaking genes for practical purposes 2. Recombinant DNA- DNA made from two or more different organisms 3. Restriction enzyme- enzymes that recognize short specific DNA sequences and that cut the DNA there 4. Plasmid- small, circular DNA molecules that can replicate independa ...
DNA and Chromosomes
... What is the relationship between DNA, chromosomes, and any organism? Drag and drop the descriptive phrase to the correct column, thereby helping us to describe the relationships between these important components of inheritance. ...
... What is the relationship between DNA, chromosomes, and any organism? Drag and drop the descriptive phrase to the correct column, thereby helping us to describe the relationships between these important components of inheritance. ...
Library construction - Center for Bioinformatics and
... Insertion of gene-source DNA into the cloning vector using the same restriction enzyme; bind the fragmented DNA with DNA ligase Introduction of cloning vector into cells (transformation by bacterial cells) Cloning of cells (and foreign genes) Identification of cell clones carrying the gene of intere ...
... Insertion of gene-source DNA into the cloning vector using the same restriction enzyme; bind the fragmented DNA with DNA ligase Introduction of cloning vector into cells (transformation by bacterial cells) Cloning of cells (and foreign genes) Identification of cell clones carrying the gene of intere ...
BIOCHEMISTRY 4.1 HOMEWORK
... insert the fragment at a site that interrupts a selectable marker (such as the tetracycline-resistance gene of pBR322). The loss of function of the interrupted gene can be used to identify clones containing recombinant plasmids with foreign DNA. With a bacteriophage vector, it is not necessary to do ...
... insert the fragment at a site that interrupts a selectable marker (such as the tetracycline-resistance gene of pBR322). The loss of function of the interrupted gene can be used to identify clones containing recombinant plasmids with foreign DNA. With a bacteriophage vector, it is not necessary to do ...
Prentice hall Biology Worksheets
... Short Answer On the lines provided, list the kinds of information that can be found by knowing the sequence of a DNA molecule. 4. __________________________________________________________________________________ 5. __________________________________________________________________________________ 6 ...
... Short Answer On the lines provided, list the kinds of information that can be found by knowing the sequence of a DNA molecule. 4. __________________________________________________________________________________ 5. __________________________________________________________________________________ 6 ...
Answers to Gene technology exam 2011-10-18
... e) Cos-sites: Sequence that give single stranded base overhang, the size of the DNA between the cos sites determines if it can be packed into phage particles. ...
... e) Cos-sites: Sequence that give single stranded base overhang, the size of the DNA between the cos sites determines if it can be packed into phage particles. ...
DNA TECHNOLOGY
... 3. The process of recombinant DNA technology takes DNA from 1 organism, cuts it into small fragments, & inserts it into a host organism of the same or a different species. The host organism receiving recombinant DNA is called a transgenic organism. ...
... 3. The process of recombinant DNA technology takes DNA from 1 organism, cuts it into small fragments, & inserts it into a host organism of the same or a different species. The host organism receiving recombinant DNA is called a transgenic organism. ...
Genetic Engineering
... • Used to make millions of copies of select section of DNA • When small amount of DNA are found but large amounts are needed for analysis • Semen, blood, other tissues, long-dead specimens – DNA from all can be amplified • Thermus aquaticus – hot springs bacterium • http://www.dnalc.org/ddnalc.org/r ...
... • Used to make millions of copies of select section of DNA • When small amount of DNA are found but large amounts are needed for analysis • Semen, blood, other tissues, long-dead specimens – DNA from all can be amplified • Thermus aquaticus – hot springs bacterium • http://www.dnalc.org/ddnalc.org/r ...
DNA Cloning - MrMsciences
... • a line of genetically identical cells or individuals derived from a single ancestor • produces many copies of a piece of DNA • uses a little fraction as gene of interest • cultivates a large amount for studying functions ...
... • a line of genetically identical cells or individuals derived from a single ancestor • produces many copies of a piece of DNA • uses a little fraction as gene of interest • cultivates a large amount for studying functions ...
notes
... Bacteria provide the means • Bacteria have been vital in developing DNA technology • Thermus aquaticus (which lives in hot springs) provides DNA polymerase enzyme for PCR • Escherichia coli (which lives in our guts) provides “plasmids” (mini-chromosomes) used in cloning • 100s of bacterial species ...
... Bacteria provide the means • Bacteria have been vital in developing DNA technology • Thermus aquaticus (which lives in hot springs) provides DNA polymerase enzyme for PCR • Escherichia coli (which lives in our guts) provides “plasmids” (mini-chromosomes) used in cloning • 100s of bacterial species ...
Vocabulary 7
... • Rosalind Franklin – used Xrays to see DNA • James Watson and Francis Crick made models to determine DNA structure – double helix ...
... • Rosalind Franklin – used Xrays to see DNA • James Watson and Francis Crick made models to determine DNA structure – double helix ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
