Tag-ItTM Mutation Detection Kit for CFTR 70+6
... Cystic Fibrosis occurs when a patient has 2 mutations (could be 2 different mutations) in the gene for CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
... Cystic Fibrosis occurs when a patient has 2 mutations (could be 2 different mutations) in the gene for CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
Cells: building blocks of living organisms
... The self-replicating genetic structures of cells containing the cellular DNA that bears in its nucleotide sequence the linear array of genes. In prokaryotes, chromosomal DNA is circular, and the entire genome is carried on one chromosome. Eukaryotic genomes consist of a number of chromosomes whose D ...
... The self-replicating genetic structures of cells containing the cellular DNA that bears in its nucleotide sequence the linear array of genes. In prokaryotes, chromosomal DNA is circular, and the entire genome is carried on one chromosome. Eukaryotic genomes consist of a number of chromosomes whose D ...
Sequence variation characteristics of D
... form long moving filaments or chains, while in others they remain fixed in one position where they provide ATP directly to a site of unusually high ATP consumption, such as being packed between adjacent myofibrils in a cardiac muscle cell or wrapped tightly around the fiagellum of a sperm. Although ...
... form long moving filaments or chains, while in others they remain fixed in one position where they provide ATP directly to a site of unusually high ATP consumption, such as being packed between adjacent myofibrils in a cardiac muscle cell or wrapped tightly around the fiagellum of a sperm. Although ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... Introduction The ability to target and amplify DNA sequences of interest using the polymerase chain reaction (PCR) has revolutionized the biological sciences. PCR can be used as a diagnostic tool in pathogen detection, genotyping, screening genes for mutations and identification of single nucleotide ...
... Introduction The ability to target and amplify DNA sequences of interest using the polymerase chain reaction (PCR) has revolutionized the biological sciences. PCR can be used as a diagnostic tool in pathogen detection, genotyping, screening genes for mutations and identification of single nucleotide ...
Water SA
... In order to compare GU and CFU values for pure culture, Legionella pneumophila Serogroup 1 was cultured in Legionella selective medium GVPC (Oxoid) for 4 days at 37°C. Once the culture was ready, a bacterial suspension was prepared transferring single colonies into sterile saline solution and adjust ...
... In order to compare GU and CFU values for pure culture, Legionella pneumophila Serogroup 1 was cultured in Legionella selective medium GVPC (Oxoid) for 4 days at 37°C. Once the culture was ready, a bacterial suspension was prepared transferring single colonies into sterile saline solution and adjust ...
Schlage Open Options AD-300 AD-400 Solution Sheet
... −− SSP-EP Controller supports up to 16 PIM400485s, 8 per port, and a maximum of 64 AD-400 locks −− IP Addressable PoE PIM400-1501 supports up to 16 AD-400 devices −− 900 MHz communication between AD-400 and PIM400 for secure, AES-128 bit encrypted ...
... −− SSP-EP Controller supports up to 16 PIM400485s, 8 per port, and a maximum of 64 AD-400 locks −− IP Addressable PoE PIM400-1501 supports up to 16 AD-400 devices −− 900 MHz communication between AD-400 and PIM400 for secure, AES-128 bit encrypted ...
AN ATTEMPT TO PRODUCE RECOMBINANT HORSE INSULIN D
... *Email: [email protected] ABSTRACT The present study aimed to produce horse insulin by use of recombinant DNA technology. Because the hormones such as somatotropin, prolactin and leptin, which are practically applicable in veterinary medicine, are also peptides, they can be obtained on the basis of t ...
... *Email: [email protected] ABSTRACT The present study aimed to produce horse insulin by use of recombinant DNA technology. Because the hormones such as somatotropin, prolactin and leptin, which are practically applicable in veterinary medicine, are also peptides, they can be obtained on the basis of t ...
Properties of Mitotic and Meiotic Recombination in the
... chromatid events have the phenotype 5-FOAR HygR GenR CanS. To determine the rates of these events shown in Table 1, for each strain, we allowed cells to form single colonies at 300 C. on rich growth medium (YPD). Each colony was suspended in water, and various dilutions were plated on SD complete, ...
... chromatid events have the phenotype 5-FOAR HygR GenR CanS. To determine the rates of these events shown in Table 1, for each strain, we allowed cells to form single colonies at 300 C. on rich growth medium (YPD). Each colony was suspended in water, and various dilutions were plated on SD complete, ...
Optimal estimation of diffusion coefficients from single
... may overestimate diffusion coefficients by several times their actual values if the fluctuations are not taken into account. The increased resolution that our method offers enables us to see a clear negative correlation between a protein’s residence time on the DNA molecule and its diffusion coeffic ...
... may overestimate diffusion coefficients by several times their actual values if the fluctuations are not taken into account. The increased resolution that our method offers enables us to see a clear negative correlation between a protein’s residence time on the DNA molecule and its diffusion coeffic ...
Biosynthesis of Glucosyl Glycerol, a Compatible Solute, Using
... centrifugation at 4 °C after a 3-h induction of the mfas gene by the addition of IPTG to a final concentration of 1 mM when the optical density at 600 nm (OD600) reached 0.5–0.6. The collected pellets were resuspended in 5 mL of lysis buffer (50-mM NaH2PO4, 300-mM NaCl, 10-mM imidazole, pH 7.0) per ...
... centrifugation at 4 °C after a 3-h induction of the mfas gene by the addition of IPTG to a final concentration of 1 mM when the optical density at 600 nm (OD600) reached 0.5–0.6. The collected pellets were resuspended in 5 mL of lysis buffer (50-mM NaH2PO4, 300-mM NaCl, 10-mM imidazole, pH 7.0) per ...
1 - IPPC
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
Specific PCR Primers for the Identification of Salmonella enterica
... Salmonella is one of the most common pathogens and a major cause of foodborne diseases in human worldwide (1,2,3). Contaminated poultry products have been identified as the principal sources of Salmonella leading to foodborne illness in human(4.5). The most common serotypes of Salmonella isolated fr ...
... Salmonella is one of the most common pathogens and a major cause of foodborne diseases in human worldwide (1,2,3). Contaminated poultry products have been identified as the principal sources of Salmonella leading to foodborne illness in human(4.5). The most common serotypes of Salmonella isolated fr ...
- Wiley Online Library
... emerged as crucial players in these processes. Cohesin is required for holding sister chromatids together whereas condensin, together with topoisomerase II, has an important role in organizing individual axes of sister chromatids prior to their segregation during anaphase. SMC and kleisin complexes ...
... emerged as crucial players in these processes. Cohesin is required for holding sister chromatids together whereas condensin, together with topoisomerase II, has an important role in organizing individual axes of sister chromatids prior to their segregation during anaphase. SMC and kleisin complexes ...
view - Association for Computational Linguistics
... There are 7,905,853,580,625 possible symmetric substitution ciphers in a 26 letter language like English. Even with the efficient means of scoring ciphers shown in section 8 (which can calculate a full cipher’s score in ∼ 300 microseconds) the brute force solution would take over 75 years of computi ...
... There are 7,905,853,580,625 possible symmetric substitution ciphers in a 26 letter language like English. Even with the efficient means of scoring ciphers shown in section 8 (which can calculate a full cipher’s score in ∼ 300 microseconds) the brute force solution would take over 75 years of computi ...
Lecture 13 Electrophoresis (Part-I)
... Troubleshooting : The pattern of the protein bands on polyacrylamide gel depend on several factors. As a result several types of defects are observed. Now we will discuss few of these defects and responsible factors. 1. Smiling : Uneven heating of the gel causes differential migration of proteins, w ...
... Troubleshooting : The pattern of the protein bands on polyacrylamide gel depend on several factors. As a result several types of defects are observed. Now we will discuss few of these defects and responsible factors. 1. Smiling : Uneven heating of the gel causes differential migration of proteins, w ...
Locked Nucleic Acid (LNA™)
... LNA™ has been proven to be a powerful tool in many molecular biological applications in which standard DNA oligonucleotides or RNA riboprobes do not show sufficient affinity or specificity. LNA™ can be used to overcome the difficulties of studying very short sequences and has greatly improved, and i ...
... LNA™ has been proven to be a powerful tool in many molecular biological applications in which standard DNA oligonucleotides or RNA riboprobes do not show sufficient affinity or specificity. LNA™ can be used to overcome the difficulties of studying very short sequences and has greatly improved, and i ...
Post-PCR sterilization: a method to control carryover contamination
... distinguishes amplicon from true target and primer oligonucleotides would be advantageous as a pre-PCR sterilization technique if the enzyme is also compatible with the PCR. With post-PCR sterilization, all of the PCR reagents can be present since polymerase activity, primer integrity, and true targ ...
... distinguishes amplicon from true target and primer oligonucleotides would be advantageous as a pre-PCR sterilization technique if the enzyme is also compatible with the PCR. With post-PCR sterilization, all of the PCR reagents can be present since polymerase activity, primer integrity, and true targ ...
A Comparative Study on the Recovery of EcoRI Endonuclease from
... Restriction enzymes have extensive applications in recombinant DNA technology. They are used in the preparation of recombinant molecules, and they provide an attractive system for the analysis of sequence specific DNA-protein interactions1 . Escherichia coli RI (EcoRI) endonuclease is a well-known r ...
... Restriction enzymes have extensive applications in recombinant DNA technology. They are used in the preparation of recombinant molecules, and they provide an attractive system for the analysis of sequence specific DNA-protein interactions1 . Escherichia coli RI (EcoRI) endonuclease is a well-known r ...
Telomere maintenance without telomerase
... recombination can replenish terminal G-rich telomeric tracts that would otherwise shorten in the absence of telomerase, as well as disperse and amplify sub-telomeric repeat elements. Investigation of the genetic requirements for this process have revealed that at least two dierent recombination pat ...
... recombination can replenish terminal G-rich telomeric tracts that would otherwise shorten in the absence of telomerase, as well as disperse and amplify sub-telomeric repeat elements. Investigation of the genetic requirements for this process have revealed that at least two dierent recombination pat ...
Chapter 4
... Dunnen et al., 2000] for unambiguous sequence variant descriptions used in clinical reports, literature and genetic databases. To check and interpret these descriptions the Mutalyzer program suite [Wildeman et al., 2008] has been built with as main purpose the automatic checking disambiguation and c ...
... Dunnen et al., 2000] for unambiguous sequence variant descriptions used in clinical reports, literature and genetic databases. To check and interpret these descriptions the Mutalyzer program suite [Wildeman et al., 2008] has been built with as main purpose the automatic checking disambiguation and c ...
The Molecular Basis of Imidazolinone Herbicide Resistance in
... products of the biosynthetic pathway valine, leucine, and isoleucine. ALS from maize has been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of ant ...
... products of the biosynthetic pathway valine, leucine, and isoleucine. ALS from maize has been partially purified and characterized (Muhitch et al., 1987). Since ALS is a labile enzyme it is very difficult to purify the active protein to homogeneity for further characterization and preparation of ant ...
to view
... pollinators mistake them as female.Therefore in the process of pseudocopulation they pollinate the flower. QUESTION BANK /BIOLOGY/XII ...
... pollinators mistake them as female.Therefore in the process of pseudocopulation they pollinate the flower. QUESTION BANK /BIOLOGY/XII ...
Control of DNA excision efficiency in Paramecium
... micronuclei undergo meiosis, whereas the macronuclei degenerate. The fusion of two gametic nuclei produces a zygotic nucleus. This nucleus divides twice and the daughter nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromos ...
... micronuclei undergo meiosis, whereas the macronuclei degenerate. The fusion of two gametic nuclei produces a zygotic nucleus. This nucleus divides twice and the daughter nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromos ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.