KAIE LOKK Comparative genome-wide DNA methylation
... base. Methylated cytosines have a crucial role in mammalian development, and the proportion of methylated CpG sites can vary greatly over a genome. Repeated sequences tend to be the most heavily methylated, while CpG-rich regulatory regions are almost exclusively unmethylated in all human tissues. A ...
... base. Methylated cytosines have a crucial role in mammalian development, and the proportion of methylated CpG sites can vary greatly over a genome. Repeated sequences tend to be the most heavily methylated, while CpG-rich regulatory regions are almost exclusively unmethylated in all human tissues. A ...
Phylogenetic analysis of members of the genus Rickettsia using the
... al., 1997). When the degree of infection was optimal, as estimated by Gimenez staining, rickettsiae were harvested and cultures were centrifuged at 12 000 g for 10 min, resuspended in medium and stored at k70 mC until nucleic acid purification was performed. Nucleic acid purification and PCR amplifi ...
... al., 1997). When the degree of infection was optimal, as estimated by Gimenez staining, rickettsiae were harvested and cultures were centrifuged at 12 000 g for 10 min, resuspended in medium and stored at k70 mC until nucleic acid purification was performed. Nucleic acid purification and PCR amplifi ...
Kinetic studies of human tyrosyl
... CACATGTTCCCATGC-3¢ were used as the forward and reverse primers. Similarly, oligonucleotides of 5¢-AAGTAT AACTCTCGAGCCCTCCACATCAAGG-3¢ and 5¢-GG AGGGCACCCACATGTTCCCATGC-3¢ were used as primers to generate the huTDPND174 variant. Generation of expression constructs to produce wildtype and mutant huma ...
... CACATGTTCCCATGC-3¢ were used as the forward and reverse primers. Similarly, oligonucleotides of 5¢-AAGTAT AACTCTCGAGCCCTCCACATCAAGG-3¢ and 5¢-GG AGGGCACCCACATGTTCCCATGC-3¢ were used as primers to generate the huTDPND174 variant. Generation of expression constructs to produce wildtype and mutant huma ...
Microplate-Based Pathlength Correction Method for Photometric
... measured with a Multiskan GO microplate spectrophotometer, and the pathlength correction was performed by SkanIt Software. The results of the 96-well plate are shown in Figure 4 and the results of the 384-well plate are shown in Figure 5. ...
... measured with a Multiskan GO microplate spectrophotometer, and the pathlength correction was performed by SkanIt Software. The results of the 96-well plate are shown in Figure 4 and the results of the 384-well plate are shown in Figure 5. ...
Chapter 3 Transposon Mutagenesis of Rhodobacter sphaeroides
... briefly explain mutagenesis and the protocol that we will be using. Mutagenesis The study of any microorganism's properties can be greatly enhanced by the generation of mutations in genes of interest. Creation of mutations and subsequent genetic mapping can elucidate the identity, relative size, num ...
... briefly explain mutagenesis and the protocol that we will be using. Mutagenesis The study of any microorganism's properties can be greatly enhanced by the generation of mutations in genes of interest. Creation of mutations and subsequent genetic mapping can elucidate the identity, relative size, num ...
Discovering the Distribution of Palindromic Sequences in the
... plot the sequential lengths (sequence x length) of both the Primary Assembly and mRNA palindromes (see figures 1-2). Each respective plot’s number of palindromes was counted. The SMAD4 mRNA .fasta file and the mutation-list on the .csv file were run through a Java program which created and saved 15 ...
... plot the sequential lengths (sequence x length) of both the Primary Assembly and mRNA palindromes (see figures 1-2). Each respective plot’s number of palindromes was counted. The SMAD4 mRNA .fasta file and the mutation-list on the .csv file were run through a Java program which created and saved 15 ...
Engineered bacteriophage-defence systems in bioprocessing
... A wide variety of engineered phage-resistance systems that target different stages in the lytic life cycle have been constructed using information that has been garnered from the examination of sequence data (FIG. 1). Although this review will be focused on dairy microorganisms, the general principl ...
... A wide variety of engineered phage-resistance systems that target different stages in the lytic life cycle have been constructed using information that has been garnered from the examination of sequence data (FIG. 1). Although this review will be focused on dairy microorganisms, the general principl ...
Chapter 29 Slides
... by transfection - injection of plasmid DNA into recipient cells • Plasmids can be injected into fertilized eggs in mice • Expression is usually variable, because the gene is inserted randomly • Growth hormone transfection produces mice that are very large! ...
... by transfection - injection of plasmid DNA into recipient cells • Plasmids can be injected into fertilized eggs in mice • Expression is usually variable, because the gene is inserted randomly • Growth hormone transfection produces mice that are very large! ...
Clin Infect Dis. - Repositorio Académico UPC
... peaks were automatically calculated by the Opticon Monitor software 115, which plotted the negative derivative of fluorescence with respect to temperature (–d(F)/dT vs T). To make sure that we did not have inhibition of amplification because of the presence of contaminants in stool samples, we rando ...
... peaks were automatically calculated by the Opticon Monitor software 115, which plotted the negative derivative of fluorescence with respect to temperature (–d(F)/dT vs T). To make sure that we did not have inhibition of amplification because of the presence of contaminants in stool samples, we rando ...
The genomic landscape of meiotic crossovers and gene
... tetrads were generated by crossing qrt1 in a Col background to qrt1 in a Ler background, and then using single pollen tetrads from the F1 hybrids to fertilize a Cvi male sterile pollen receptor. Tetrad offspring are heterozygous, with one recombinant Col-Ler genome and one Cvi genome. 10 DH lines we ...
... tetrads were generated by crossing qrt1 in a Col background to qrt1 in a Ler background, and then using single pollen tetrads from the F1 hybrids to fertilize a Cvi male sterile pollen receptor. Tetrad offspring are heterozygous, with one recombinant Col-Ler genome and one Cvi genome. 10 DH lines we ...
Applied Microbiology and Biotechnology
... 46,239 Da. The first 27 N-terminal amino acids form a cleavable signal peptide with a putative processing site AAA↓ADWQ (Nielsen et al. 1997). The amino acid sequence of the mature protein contains the active site motif RHGXRXP that is shared by other histidine acid phosphatases and phytases (Mitche ...
... 46,239 Da. The first 27 N-terminal amino acids form a cleavable signal peptide with a putative processing site AAA↓ADWQ (Nielsen et al. 1997). The amino acid sequence of the mature protein contains the active site motif RHGXRXP that is shared by other histidine acid phosphatases and phytases (Mitche ...
Hailey Spelman - Determining Cellular Fate: Pre- and Postnatal Methylation Effects on Gene Expression
... off genes that are functional and thus contribute to the final phenotype. Examples of the effect of simple measures on methylation patterns during prenatal development are clear in their ability to change phenotypic expression. Based on this idea, if scientists could target methylation of specific g ...
... off genes that are functional and thus contribute to the final phenotype. Examples of the effect of simple measures on methylation patterns during prenatal development are clear in their ability to change phenotypic expression. Based on this idea, if scientists could target methylation of specific g ...
Full-Text PDF
... to different antibiotic classes and can be used to direct therapy, but are slow and take up to 72 h. In the absence of rapid results, treatment will often use broad-spectrum antibiotics on an empirical basis, which may contribute to increased drug resistance. The emergence of multi-drug-resistant (M ...
... to different antibiotic classes and can be used to direct therapy, but are slow and take up to 72 h. In the absence of rapid results, treatment will often use broad-spectrum antibiotics on an empirical basis, which may contribute to increased drug resistance. The emergence of multi-drug-resistant (M ...
biology 160 laboratory objectives practical one
... BIOLOGY 160 LABORATORY OBJECTIVES PRACTICAL TWO ...
... BIOLOGY 160 LABORATORY OBJECTIVES PRACTICAL TWO ...
Corning® Epoxide Coated Slides Instruction Manual
... print tests must be done in order to ensure that the desired spot density and array uniformity is achievable. Once probe DNA is dissolved in a spotting medium, it is very difficult to recover it for reconstitution in a different solvent. The Pronto! Epoxide Spotting Solution (Cat. No. 40047) is prov ...
... print tests must be done in order to ensure that the desired spot density and array uniformity is achievable. Once probe DNA is dissolved in a spotting medium, it is very difficult to recover it for reconstitution in a different solvent. The Pronto! Epoxide Spotting Solution (Cat. No. 40047) is prov ...
Enzyme Mechanisms - Illinois Institute of Technology
... Incorporates DNA replication as an analytical tool for determining sequence Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesi ...
... Incorporates DNA replication as an analytical tool for determining sequence Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesi ...
Quantitative Analysis of the Kinetics of End
... structure. The ATP concentration is maintained at high levels so that each DNA-bound RecA monomer is functioning at its kcat. The kcat employed is that observed in the presence of longer random sequence ssDNA molecules. Under conditions used in this series of experiments, the kcat for ATP hydrolysis ...
... structure. The ATP concentration is maintained at high levels so that each DNA-bound RecA monomer is functioning at its kcat. The kcat employed is that observed in the presence of longer random sequence ssDNA molecules. Under conditions used in this series of experiments, the kcat for ATP hydrolysis ...
background of the invention
... important subject for biosensor developers to separate, purify or design a biologically active molecule by a biochemical method, and integrate a precise and quick responding physical transducer to form a biosensor reaction system. At present, the development of biosensors has not reached the mature ...
... important subject for biosensor developers to separate, purify or design a biologically active molecule by a biochemical method, and integrate a precise and quick responding physical transducer to form a biosensor reaction system. At present, the development of biosensors has not reached the mature ...
Microsoft Word (Chapter 3) - DORAS
... siderophores, haem and haemoglobin. After incubation for 24 – 48 hours at 30oC, halos of growth appear around wells where the test solutions are being utilised. Ferric chloride is generally added to one of the wells for each bioassay as a positive control. Strains with the ability to produce and uti ...
... siderophores, haem and haemoglobin. After incubation for 24 – 48 hours at 30oC, halos of growth appear around wells where the test solutions are being utilised. Ferric chloride is generally added to one of the wells for each bioassay as a positive control. Strains with the ability to produce and uti ...
ARTICLES - Weizmann Institute of Science
... binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it is not known whether these sequence preferences have a significant influence on nucleosome position in vivo, a ...
... binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it is not known whether these sequence preferences have a significant influence on nucleosome position in vivo, a ...
pyrimidine
... Laser fluorescence detection allows for primer identification in real time An automated sequencing machine can handle 4500 bases/hour That’s one of the technologies that has made large-scale sequencing projects like the human genome project possible ...
... Laser fluorescence detection allows for primer identification in real time An automated sequencing machine can handle 4500 bases/hour That’s one of the technologies that has made large-scale sequencing projects like the human genome project possible ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.