Development of Co-Dominant Amplified Polymorphic Sequence
... The original cross to generate the RILs utilized upland japonica cv. Moroberekan and susceptible indica cv. CO39. The intersubspecies cross was predicted to possess sufficiently high rates of polymorphism to efficiently generate CAPS markers. Eight CAPS markers, including S12886, were found at the P ...
... The original cross to generate the RILs utilized upland japonica cv. Moroberekan and susceptible indica cv. CO39. The intersubspecies cross was predicted to possess sufficiently high rates of polymorphism to efficiently generate CAPS markers. Eight CAPS markers, including S12886, were found at the P ...
An improved protocol for pulsed-field gel electrophoresis typing of
... Typing of the micro-organism can be useful in the detection and control of epidemic outbreaks and endemic situations, and in the characterization of recurrences (Alonso et al., 2001; Kato et al., 1996). Different approaches have been used for typing bacteria, although PFGE has traditionally been the ...
... Typing of the micro-organism can be useful in the detection and control of epidemic outbreaks and endemic situations, and in the characterization of recurrences (Alonso et al., 2001; Kato et al., 1996). Different approaches have been used for typing bacteria, although PFGE has traditionally been the ...
A Physical Gene Map of the Bacteriophage P22 Late
... of P22 DNA by EcoRI has been shown to be a consequence of P22 DNA packaging (Jackson et al. 1978a). Because mature P22 DNA molecules are linear and circularly permuted, the cleavage map is circular (Jackson et al., 1978b). These mature chromosomes are generated by sequential headful packaging from a ...
... of P22 DNA by EcoRI has been shown to be a consequence of P22 DNA packaging (Jackson et al. 1978a). Because mature P22 DNA molecules are linear and circularly permuted, the cleavage map is circular (Jackson et al., 1978b). These mature chromosomes are generated by sequential headful packaging from a ...
Jigsaw handout - the Biology Scholars Program Wiki
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
Structure, expression and chromosomal location of the Oct
... kb. It is unclear what the larger fragment represents because there is no BssHI1 site in the gene. However, there is a BssHII site in a cosmid containing Oct-4 (see below). The same pulsed field gel was then probed with pH2IIa, a generic probe for class I genes (Steinmetz et al., 1981). Although pre ...
... kb. It is unclear what the larger fragment represents because there is no BssHI1 site in the gene. However, there is a BssHII site in a cosmid containing Oct-4 (see below). The same pulsed field gel was then probed with pH2IIa, a generic probe for class I genes (Steinmetz et al., 1981). Although pre ...
Parallel Genetic and Phenotypic Evolution of DNA Superhelicity in
... Losos et al. 1998; Huey et al. 2000). Parallel changes sometimes extend even to the genome, as has been described for some viral and bacterial pathogens (Crandall et al. 1999; Reid et al. 2000). The parallel evolution of DNA superhelicity in the long-term experiment with E. coli provides therefore h ...
... Losos et al. 1998; Huey et al. 2000). Parallel changes sometimes extend even to the genome, as has been described for some viral and bacterial pathogens (Crandall et al. 1999; Reid et al. 2000). The parallel evolution of DNA superhelicity in the long-term experiment with E. coli provides therefore h ...
Genomic Analysis of Arabidopsis Thaliana
... In addition to the many advantages described above, the rapid rise of Arabidopsis as a preferred plant model is based upon its small genome size and few repetitive sequences. The framework for Arabadopsis genomics was set by early kinetic analyses of total Arabidopsis DNA in 1984. Most of the genome ...
... In addition to the many advantages described above, the rapid rise of Arabidopsis as a preferred plant model is based upon its small genome size and few repetitive sequences. The framework for Arabadopsis genomics was set by early kinetic analyses of total Arabidopsis DNA in 1984. Most of the genome ...
File
... processes responsible for the replication and expression of genetic information. Furthermore, the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most signi ...
... processes responsible for the replication and expression of genetic information. Furthermore, the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most signi ...
Transcription
... metabolic activities. In present day cells, DNA stores information and proteins perform catalysis, with RNA as the intermediate between DNA and protein. One can imagine a time when there was no DNA or protein, just RNA performing both functions: this is the RNA World hypothesis. • Very long ago, at ...
... metabolic activities. In present day cells, DNA stores information and proteins perform catalysis, with RNA as the intermediate between DNA and protein. One can imagine a time when there was no DNA or protein, just RNA performing both functions: this is the RNA World hypothesis. • Very long ago, at ...
PPT - Altogen Biosystems
... Figure 2. Protein expression of Lamin A in PC-12 cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into PC-12 cells following Altogen Biosystems transfection protocol. At 72 hours posttransfection the cells were analyzed by Western Blot for protein expression levels ( ...
... Figure 2. Protein expression of Lamin A in PC-12 cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into PC-12 cells following Altogen Biosystems transfection protocol. At 72 hours posttransfection the cells were analyzed by Western Blot for protein expression levels ( ...
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria
... Küpferet al., 2006).In addition,V. anguillarumhas a very close phylogenetic relationshipwith other Vibrio species based on genetic analysis of 16S rDNA and recA regions (Kita-Tsukamoto et al., 1993; Urakawa et al., 1997; Thompson et al., 2004).Thus, the 16S rDNA region may not be the most suitable f ...
... Küpferet al., 2006).In addition,V. anguillarumhas a very close phylogenetic relationshipwith other Vibrio species based on genetic analysis of 16S rDNA and recA regions (Kita-Tsukamoto et al., 1993; Urakawa et al., 1997; Thompson et al., 2004).Thus, the 16S rDNA region may not be the most suitable f ...
Repair of Site-Specific DNA Double-Strand Breaks in
... restore the I-SceI recognition site. We detected 33.42% of reads (2563 reads) from GU.US, 17.56% (1672 reads) from DU.GUS, and 24.86% (1783 reads) from IU.GUS that displayed the original I-SceI site (Figure 4B; Supplemental Tables 1 and 2). Previously, transgenic barley plants showed constitutive ex ...
... restore the I-SceI recognition site. We detected 33.42% of reads (2563 reads) from GU.US, 17.56% (1672 reads) from DU.GUS, and 24.86% (1783 reads) from IU.GUS that displayed the original I-SceI site (Figure 4B; Supplemental Tables 1 and 2). Previously, transgenic barley plants showed constitutive ex ...
Real time PCR and it`s role in diagnosis
... Improving our understanding of the biology of the Plasmodium falciparum parasite is of extreme importance if we are to combat human malaria.This parasite uses the process of antigenic variation to expose the human immune system to continually changing antigens on the surface of infected red blood ce ...
... Improving our understanding of the biology of the Plasmodium falciparum parasite is of extreme importance if we are to combat human malaria.This parasite uses the process of antigenic variation to expose the human immune system to continually changing antigens on the surface of infected red blood ce ...
Acetate kinase from CIostridiurn acetobutylicurn : a highly specific
... phosphotransbutyrylase have been purified and characterized (Hartmanis, 1987; Wiesenborn et al., 1989). The purified butyrate kinase also shows a low reaction rate with acetate as substrate, but the high level of acetate and butyrate kinase activities in crude extracts (Andersch et al., 1983; Hartma ...
... phosphotransbutyrylase have been purified and characterized (Hartmanis, 1987; Wiesenborn et al., 1989). The purified butyrate kinase also shows a low reaction rate with acetate as substrate, but the high level of acetate and butyrate kinase activities in crude extracts (Andersch et al., 1983; Hartma ...
Structure and function of the GINS complex, a key component of the
... [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 and Ser622 is essential for cell viability, substituting these two residues with non-phosphorylatable alanine residues blocks entry into S-phase. The effect of the phosphorylation of Sld3 is to generate a binding site from another essential replica ...
... [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 and Ser622 is essential for cell viability, substituting these two residues with non-phosphorylatable alanine residues blocks entry into S-phase. The effect of the phosphorylation of Sld3 is to generate a binding site from another essential replica ...
Fragaria multicipita - DigitalCommons@University of Nebraska
... F2n/R2. Fig. 1 (lanes labeled A through F) shows results from TaqI RFLP analysis of the cloned rDNA inserts. The three separate experiments yielded the same results; two classes of TaqI RFLP patterns were observed. The class I pattern is seen in Fig. 1, lanes A, C and E, and the class II pattern is ...
... F2n/R2. Fig. 1 (lanes labeled A through F) shows results from TaqI RFLP analysis of the cloned rDNA inserts. The three separate experiments yielded the same results; two classes of TaqI RFLP patterns were observed. The class I pattern is seen in Fig. 1, lanes A, C and E, and the class II pattern is ...
CH Zinc Fingers As DNA Binding Domains
... key for zinc finger peptides to specifically bind to the DNA duplexes. Indeed, the arginine-guanine contact, whose association is the greatest of all the combinations, is quite often present in zinc finger-DNA complexes (Fig. 4B and C). However, each amino acid residue of the zinc finger peptides do ...
... key for zinc finger peptides to specifically bind to the DNA duplexes. Indeed, the arginine-guanine contact, whose association is the greatest of all the combinations, is quite often present in zinc finger-DNA complexes (Fig. 4B and C). However, each amino acid residue of the zinc finger peptides do ...
Alus
... and Alus • An estimated 500-2,000 Alu elements are restricted to the human genome. The vast majority of Alu insertions occur in non-coding regions and are thought to be evolutionarily neutral. However, an Alu insertion in the NF-1 gene is responsible for neurofibromatosis I, Alu insertions in intron ...
... and Alus • An estimated 500-2,000 Alu elements are restricted to the human genome. The vast majority of Alu insertions occur in non-coding regions and are thought to be evolutionarily neutral. However, an Alu insertion in the NF-1 gene is responsible for neurofibromatosis I, Alu insertions in intron ...
DNA Base Composition, DNA-DNA Homology and Long
... respectively. These data are in good agreement with the previous reports of about 38 to 42 mol % G + C within these species (Deibel & Seeley, 1974; Kilpper-Balz et al., 1982). The results of DNA-DNA hybridization experiments are shown in Table 2. Streptococcus salivarius and S. therrnophilus strains ...
... respectively. These data are in good agreement with the previous reports of about 38 to 42 mol % G + C within these species (Deibel & Seeley, 1974; Kilpper-Balz et al., 1982). The results of DNA-DNA hybridization experiments are shown in Table 2. Streptococcus salivarius and S. therrnophilus strains ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.