Anti-Rat Ciliary Neurotrophic Factor (CNTF) Developed in Goat IgG
... CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons. CNTF is also a survival factor for developing ciliary ganglionic neurons.1 Rat CNTF was initially purified from rat sciatic nerve extract.1 The rat CNTF coding domain consists of one intron. 1 The ...
... CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons. CNTF is also a survival factor for developing ciliary ganglionic neurons.1 Rat CNTF was initially purified from rat sciatic nerve extract.1 The rat CNTF coding domain consists of one intron. 1 The ...
A New Species of Actinomycete, Amycolata alni
... Cultural properties. The cultural characteristics of Amycolata autotrophica and Amycolata alni strains were similar, corresponding to those given in the description of Amycolata autotrophica (19). All of the strains produced aerial mycelia which were white to cream colored. However, the color of the ...
... Cultural properties. The cultural characteristics of Amycolata autotrophica and Amycolata alni strains were similar, corresponding to those given in the description of Amycolata autotrophica (19). All of the strains produced aerial mycelia which were white to cream colored. However, the color of the ...
Expressway™ Cell-Free E. coli Expression System
... The Expressway™ Cell-Free E. coli Expression System uses an optimized E. coli extract, a reaction buffer containing an ATP regenerating system, and amino acids to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis ...
... The Expressway™ Cell-Free E. coli Expression System uses an optimized E. coli extract, a reaction buffer containing an ATP regenerating system, and amino acids to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis ...
The msh2 Gene of Schizosaccharomyces pombe Is
... DNA-binding domain. Cytological analysis revealed that during meiotic prophase of msh2-defective cells, chromosomal structures were frequently formed; such structures are rarely found in the wild type. Our data show that besides having a function in mismatch repair, S. pombe msh2 is required for cor ...
... DNA-binding domain. Cytological analysis revealed that during meiotic prophase of msh2-defective cells, chromosomal structures were frequently formed; such structures are rarely found in the wild type. Our data show that besides having a function in mismatch repair, S. pombe msh2 is required for cor ...
Assessment of the mosaic structure in the
... single amino acid mutations in the EPIYA/T segments (Supplement S1). H. pylori strain HJM6 and HJM12 possess an EPIYA/T-AABC genotype, whereas H. pylori strain HJM11 has an EPIYA/T-ABD genotype corresponding to the East Asian type (Supplement S1), including and a 39 bp-deletion in the pre-EPIYA/T re ...
... single amino acid mutations in the EPIYA/T segments (Supplement S1). H. pylori strain HJM6 and HJM12 possess an EPIYA/T-AABC genotype, whereas H. pylori strain HJM11 has an EPIYA/T-ABD genotype corresponding to the East Asian type (Supplement S1), including and a 39 bp-deletion in the pre-EPIYA/T re ...
Protein Folding and Expression
... protein in secreted form. This system allows high yield of active proteins and is wellsuited for expression of eukaryotic proteins. The Brevibacillus system is nearly free of proteases, which facilitates production of intact protein products. Examples of successfully expressed proteins can be seen i ...
... protein in secreted form. This system allows high yield of active proteins and is wellsuited for expression of eukaryotic proteins. The Brevibacillus system is nearly free of proteases, which facilitates production of intact protein products. Examples of successfully expressed proteins can be seen i ...
Single-Molecule PCR in a Picowell Array Simultaneously
... ABSTRACT For the first time, we amplified single DNA-molecules (“Digital PCR”) randomly distributed in a picowell array and simultaneously immobilized the generated PCR-products to the surface of a PDMS coverslide (“solid-phase PCR”) which was used as sealing of the picowells during PCR. First, by t ...
... ABSTRACT For the first time, we amplified single DNA-molecules (“Digital PCR”) randomly distributed in a picowell array and simultaneously immobilized the generated PCR-products to the surface of a PDMS coverslide (“solid-phase PCR”) which was used as sealing of the picowells during PCR. First, by t ...
Chromatin DNA Methylayion
... Model 2: The steady state methylation pattern is a dynamic equilibrium between methylase and demethylase activities CH3 ...
... Model 2: The steady state methylation pattern is a dynamic equilibrium between methylase and demethylase activities CH3 ...
The plant genome`s methylation status and response to stress
... Differences in DNA methylation within a single genotype can also contribute to heritable trait differences that can be selected. In maize, alleles of B1 and the pericarp color gene P1, another transcriptional regulator of the flavonoid biosynthetic pathway, can have identical sequences but different ...
... Differences in DNA methylation within a single genotype can also contribute to heritable trait differences that can be selected. In maize, alleles of B1 and the pericarp color gene P1, another transcriptional regulator of the flavonoid biosynthetic pathway, can have identical sequences but different ...
Sequence Heterogeneities Among 16s
... different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons within a single organism (Escherichia coli). These sequences have been analyzed by neighbor-joining, maximum ...
... different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons within a single organism (Escherichia coli). These sequences have been analyzed by neighbor-joining, maximum ...
Gene Detection Systems Catalog
... Our mission is to be one of the most reliable suppliers of reagents and reagent systems used in genetic research technology applications worldwide. Gene Link, Inc. is privately held biotechnology company incorporated in the State of New York dedicated to fundamental and applied research on genetic/b ...
... Our mission is to be one of the most reliable suppliers of reagents and reagent systems used in genetic research technology applications worldwide. Gene Link, Inc. is privately held biotechnology company incorporated in the State of New York dedicated to fundamental and applied research on genetic/b ...
fulltext
... and the dehydrated state. It is known that these factors may cause damage to DNA. It has recently been shown that single and double DNA strand breaks occur when tardigrades are maintained for a long time in the anhydrobiotic state. This may suggest that perhaps tardigrades rely on efficient DNA repa ...
... and the dehydrated state. It is known that these factors may cause damage to DNA. It has recently been shown that single and double DNA strand breaks occur when tardigrades are maintained for a long time in the anhydrobiotic state. This may suggest that perhaps tardigrades rely on efficient DNA repa ...
Biotechnology Timeline
... engineering techniques to cut and paste DNA using restriction enzymes. (1977 sees the first expression of a human gene in bacteriahuman insulin in E. coli.) ...
... engineering techniques to cut and paste DNA using restriction enzymes. (1977 sees the first expression of a human gene in bacteriahuman insulin in E. coli.) ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
... events because the telomere reFigure 1 Probes H49 and JL8 define a pair of size-polymorphic homologous chromosomes. (A) peat arrays of the T. cruzi chroSeparation of T. cruzi chromosomal bands by PFGE and staining with ethidium bromide. The mosomes are short, ranging in arabic and roman numerals ind ...
... events because the telomere reFigure 1 Probes H49 and JL8 define a pair of size-polymorphic homologous chromosomes. (A) peat arrays of the T. cruzi chroSeparation of T. cruzi chromosomal bands by PFGE and staining with ethidium bromide. The mosomes are short, ranging in arabic and roman numerals ind ...
MicroReview Paradigms of plasmid organization
... bacterial cell will tend to recombine, especially when prompted by DNA damage and, for circular molecules, this is a problem because it can cause dimerization, which prevents separation of the monomers at partitioning. So, for both high- and low-copy-number plasmids, a multimer resolution system (mr ...
... bacterial cell will tend to recombine, especially when prompted by DNA damage and, for circular molecules, this is a problem because it can cause dimerization, which prevents separation of the monomers at partitioning. So, for both high- and low-copy-number plasmids, a multimer resolution system (mr ...
7nQ Jj I f NO "7^07 - UNT Digital Library
... chapters concerning topics about DNA, mitosis and meiosis, recombinant DNA ...
... chapters concerning topics about DNA, mitosis and meiosis, recombinant DNA ...
INFINITI CYP450 2C19+ Assay
... Cytochrome P450 (CYP450) 2C19 is a member of the hepatic microsomal enzymes which is involved in the xenobiotics metabolism. CYP450 2C19 (CYP2C19) is a highly polymorphic liver enzyme of the liver and is involved in the metabolism and elimination of many commonly prescribed drugs including antidepre ...
... Cytochrome P450 (CYP450) 2C19 is a member of the hepatic microsomal enzymes which is involved in the xenobiotics metabolism. CYP450 2C19 (CYP2C19) is a highly polymorphic liver enzyme of the liver and is involved in the metabolism and elimination of many commonly prescribed drugs including antidepre ...
CTY Genetics Syllabus
... • HexBot Battles of the lac operon (30 minutes) Robot simulation of effect of inducers/repressors • Scientific Drawing: Tracing genes from DNA-RNA-Protein Structure with ID of regulator locations in each step (20 minutes) • Lecture/Class Notes- Assays for gene expression- microarray and qPCR (20 m ...
... • HexBot Battles of the lac operon (30 minutes) Robot simulation of effect of inducers/repressors • Scientific Drawing: Tracing genes from DNA-RNA-Protein Structure with ID of regulator locations in each step (20 minutes) • Lecture/Class Notes- Assays for gene expression- microarray and qPCR (20 m ...
Review A model for chromosome structure during the mitotic
... Figures 1^11 (overleaf). Diagrammatic representations of chromosome structure through the mitotic cell cycle. Drawings are not to scale. Telophase/G1 . (A) Longitudinal view of a segment of a decondensing chromosome during telophase or a decondensed chromosome during G1. The anaphase chromosome core ...
... Figures 1^11 (overleaf). Diagrammatic representations of chromosome structure through the mitotic cell cycle. Drawings are not to scale. Telophase/G1 . (A) Longitudinal view of a segment of a decondensing chromosome during telophase or a decondensed chromosome during G1. The anaphase chromosome core ...
A-level Biology Previous essay titles and mark schemes
... E. Evolution of antibiotic-resistance in Bacteria DNA. Genetic diversity because of ...
... E. Evolution of antibiotic-resistance in Bacteria DNA. Genetic diversity because of ...
K - Romanian Biotechnological Letters
... time interval and the ramping. Using a computer controlled device, only few parameters must be defined, every cycle consisting of a succession of steps (forward migration, pause, reverse migration, pause) being controlled by computer, according to the chosen ramp and parameters. It is obvious that, ...
... time interval and the ramping. Using a computer controlled device, only few parameters must be defined, every cycle consisting of a succession of steps (forward migration, pause, reverse migration, pause) being controlled by computer, according to the chosen ramp and parameters. It is obvious that, ...
Nanopore Unzipping of Individual DNA Hairpin Molecules
... not entail the physical coupling of the molecules under test to a force transducer, very high throughput can be achieved. We used our method to study DNA unzipping kinetics at small forces, which have not been accessed before. We find that in this regime the static unzipping times decrease exponentia ...
... not entail the physical coupling of the molecules under test to a force transducer, very high throughput can be achieved. We used our method to study DNA unzipping kinetics at small forces, which have not been accessed before. We find that in this regime the static unzipping times decrease exponentia ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.