Download Anti-Rat Ciliary Neurotrophic Factor (CNTF) Developed in Goat IgG

Anti-Rat Ciliary Neurotrophic Factor (CNTF)
Developed in Goat
IgG Fraction of Antiserum
Product No. C4085
Product Description
Anti-Rat Ciliary Neurotrophic Factor was developed in
goat using recombinant, rat ciliary neurotrophic factor
(CNTF), expressed in E. coli, as the immunogen. The
antibody is purified by Protein G affinity chromatography. Goat Anti-Rat CNTF is provided lyophilized
from phosphate buffered saline, to which no
preservatives have been added.
CNTF was initially identified as a trophic factor for
embryonic chick ciliary parasympathetic neurons. CNTF
is also a survival factor for developing ciliary ganglionic
neurons.1 Rat CNTF was initially purified from rat
sciatic nerve extract.1 The rat CNTF coding domain
consists of one intron. 1 The amino acid sequence of rat
CNTF has 85% homology to human and rabbit CNTF.1
Ciliary Neurotrophic Factor (CNTF) is produced in E.
coli by a DNA sequence encoding the rat CNTF
protein. 2
Performance
Anti-Rat CNTF is tested for its ability to neutralize the
biological activity of recombinant rat CNTF activity on
the human cell line, TF-1. The ND50 of the antibody is
defined as the concentration of antibody resulting in a
one-half maximal inhibition of bioactivity of rat,
recombinant CNTF that is present at a concentration
just high enough to elicit a maximum response.
Endotoxin:
<10 ng/vial by LAL method
Bioactivity:
ND50 = 50-100 µg/ml
Indirect ELISA: 1 µg/ml detects 0.2 ng/well of recombinant, rat CNTF.
Indirect
Immunoblotting: 2 µg/ml antibody detects recombinant,
rat CNTF at 2 ng/lane under both
reducing and non-reducing conditions
Sterility:
0.2 µm-filtered, aseptic fill
Reconstitution and Use
To one vial of lyophilized powder, add 1 ml of sterilefiltered PBS to produce a 1.0 mg/ml stock solution of
Anti-Rat CNTF. If aseptic technique is used, no further
filtration should be needed for use in cell culture
environments.
Storage
Prior to reconstitution, store at -20ºC. Reconstituted
product may be stored at 2-8ºC for a maximum of one
month. For prolonged storage, freeze in working aliquots
at -20ºC. Avoid repeated freezing and thawing.
References
1. Manthorpe, M., et al., Neurotrophic Factors,
Loughlin, S., et al., (eds.), Academic Press, California, 443 (1993).
2. Stockli, K., et al., Nature, 342, 920 (1989).
Pcs2/01
Product Information
Mass/vial:
1 mg
Immunogen:
Rat, recombinant CNTF
Host Animal:
Goat
Formulation:
Lyophilized from PBS without additives.
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