Download Ciliary Neurotrophic Factor (CNTF).

Ciliary Neurotrophic Factor (CNTF). Mouse Monoclonal Antibody
hCNTF; Ciliary Neurotrophic Factor; CNTF
BACKGROUND
CNTF is a survival factor for various neuronal cell types. Seems to prevent
the degeneration of motor axons after axotomy. CNTF is a polypeptide
hormone and nerve growth factor whose actions have mainly been studied
in the nervous system where it promotes neurotransmitter synthesis and
neurite outgrowth in certain neuronal populations including astrocytes. The
protein is a potent survival factor for neurons and oligodendrocytes and
may be relevant in reducing tissue destruction during inflammatory
attacks. A mutation in this gene, which results in aberrant splicing, leads to
ciliary neurotrophic factor deficiency, but this phenotype is not causally
related to neurologic disease. In addition to the predominant
monocistronic transcript originating from this locus, the gene is also cotranscribed with the upstream ZFP91 gene. Co-transcription from the two
loci results in a transcript that contains a complete coding region for the
zinc finger protein but lacks a complete coding region for ciliary
neurotrophic factor. CNTF has also been shown to be expressed by cells
on the bone surface, and to reduce the activity of bone forming cells,
osteoblasts.
ORDERING INFORMATION
CATALOG NUMBER
L100M
SIZE
500 µg
FORM
Unconjugated
HOST/CLONE
Mouse Clone AS23
FORMULATION
Provided as solution in phosphate
buffered saline with 0.08% sodium
azide
CONCENTRATION
See vial for concentration
ISOTYPE
IgG1
APPLICATIONS
Western Blot, Immunoprecipitation,
Enzyme Immunoassay
SPECIES REACTIVITY
Human
ACCESSION NUMBER
P26441, Human
IMMUNOGEN
Human recombinant ciliary neurotrophic factor (hCNTF).
POSITIVE CONTROL/TISSUE EXPRESSION
Specificity: Recognizes human CNTF, does not recognize human NGF or a panel of other human cytokines.
COMMENTS
Applications: ELISA: This antibody has been used as a coating antibody at 10 ug/ml in a two site ELISA with
PS2-B. Typically the ELISA has a working range of 0-20000 pg/ml and a sensitivity of 10 pg/ml when used with
biotin conjugated CNTF antibody (Cat. No. L120P). Recommended sample volumes are 25-200 μl. Recovery
of hCNTF in human plasma is typically > 85% in the ELISA (Kenney, JS, Masada, MP, and Allison AC,
Laboratory Methods in Immunology, Vol. I, 231, 1990).
Western Blot: This antibody can be used at a range
from1 to 5 μg/ml to visualize hCNTF in Western Blots with appropriate secondary reagents.
Immunoprecipitation: This antibody has been used to immunoprecipitate > 90% hCNTF in solution using a
double-antibody method. (Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980).
Last Modified
6/19/2012
PURIFICATION
SHIP CONDITIONS
For research use only. Not for use in human diagnostics or therapeutics.
Exalpha Biologicals, Inc.
2 Shaker Road, Bldg. B101
Shirley, MA 01464
Tel: 800.395.1137 Fax: 866.924.5100 www.exalpha.com [email protected]
Page 1 of 2
Cat. No. L100M
PURIFICATION
Protein A/G Chromatography
SHIP CONDITIONS
Room Temperature
STORAGE CUSTOMER
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
STABILITY
Products are stable for one year from purchase when stored properly
REFERENCES
1. Lam, A., et al. “Sequence and structural organization of the human gene encoding ciliary neurotrophic factor.”
Gene, 1991, 102, 271-276
2. Cargill, M., et al. “Characterization of single-nucleotide polymorphisms in coding regions of human genes.” Nat
Genet 1999, 22, 231-238
Last Modified
6/19/2012
For research use only. Not for use in human diagnostics or therapeutics.
Exalpha Biologicals, Inc.
2 Shaker Road, Bldg. B101
Shirley, MA 01464
Tel: 800.395.1137 Fax: 866.924.5100 www.exalpha.com [email protected]
Page 2 of 2
Cat. No. L100M