Download Recombinant Rat Ciliary Neurotrophic Factor (CNTF)

ReliaTech GmbH
Specification/DATA SHEET
Recombinant Rat Ciliary Neurotrophic Factor (CNTF)
20140814BB
FOR RESEARCH ONLY! NOT FOR HUMAN USE!
Cat.-no:
Size:
Lot. No.:
Country of origin:
R20-001S
5 µg
According to product label
Germany
Scientific Background
Gene:
Cntf
Synonyms:
Ciliary Neurotrophic Factor, CNTF
Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified
from chick embryo ocular tissue and identified as a trophic factor for
embryonic chick ciliary parasympathetic neurons in culture.
Subsequent studies have demonstrated that CNTF is a survival factor
for additional neuronal cell types including: dorsal root ganglion
sensory neurons, sympathetic ganglion neurons, embryonic motor
neurons, major pelvic ganglion neurons and hippocampal neurons.
CNTF has also been shown to prevent the degeneration of motor axons
after axotomy.
The cDNA for CNTF encodes a 199 amino acid residue polypeptide
that lacks a signal sequence. CNTF is highly conserved across species
and exhibits cross-species activities. Human and rat CNTF share
approximately 83% homology in their protein sequence. CNTF is
structurally related to IL6, IL11, LIF, and OSM. All of these four helix
bundle cytokines share gp130 as a signal transducing subunit in their
receptor complexes.
.
Sequence
AFAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLDSV
DGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPTEGDF
HQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGLFEKKLW
GLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM
Database References
Protein RefSeq:
NP_037298.1
Uniprot ID:
P20294
mRNA RefSeq:
NM_013166.1
Product Specifications
Expressed in
E.coli
Purity
> 98% by SDS-PAGE & silver stain
Endotoxin
< 0.1ng per µg of CNTF
Buffer
5 mM sodium acetate, pH 6.5
Stabilizer
None
Formulation
lyophilized
Length (aa):
199
MW:
22,7 kDa
Result by Nterminal
sequencing
AFAEQTPLTHR
Stability: The lyophilized powder although stable at room
temperature for 3 weeks, is best stored desiccated at -20°C.
Reconstituted rat CNTF should be stored in working aliquots at –
20°C. For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA).
Reconstitution: Rat CNTF should be reconstituted in PBS to a
concentration of 0.1 mg/ml. This solution can be diluted in water or
other buffer solutions or stored at -20°C.
AVOID REPEATED FREEZE AND THAW CYCLES!
References
1. Finn T and Nishi R, Perspect Dev Neurobiol 4, 1996.
2. Sendtner M et al, J Neurol Sci 124 Suppl:77-83, 1994.
3. Sendtner M et al, J Cell Sci Suppl 15, 1991.
Biological Activity: Measured in a cell proliferation assay using TF1
human erythroleukemic cells [Kitamura T et al, J Cell Physiol 140,
1989). The ED50 for this effect is typically 3 - 15 ng/mL.
ReliaTech GmbH
Phone: +49 (0)5331 8586 987  Fax: +49 (0)5331 8586 989  E-mail: [email protected]  web: www.reliatech.de
Location: Lindener Str. 15, 38300 Wolfenbüttel, Germany
1
ReliaTech GmbH
Specification/DATA SHEET
Recombinant Rat Ciliary Neurotrophic Factor (CNTF)
rC
NT
F
Handling/Application
M
50 40 -
30 -
25 20 -
15 (SDS-PAGE 15%, red.)
Fig. 1: SDS-PAGE analysis of recombinant rat CNTF. Sample was loaded in
15% SDS-polyacrylamide gel under reducing condition and stained with
Coomassie blue.
cells/ml (% of control)
250
200
150
100
RELIATech
Competitor
0
5
10
15
20
25
rCNTF [ng/ml]
Fig. 2: Proliferation assay with TF-1 cells.
ReliaTech GmbH
Phone: +49 (0)5331 8586 987  Fax: +49 (0)5331 8586 989  E-mail: [email protected]  web: www.reliatech.de
Location: Lindener Str. 15, 38300 Wolfenbüttel, Germany
2