DNA Base Composition, DNA-DNA Homology and Long
... respectively. These data are in good agreement with the previous reports of about 38 to 42 mol % G + C within these species (Deibel & Seeley, 1974; Kilpper-Balz et al., 1982). The results of DNA-DNA hybridization experiments are shown in Table 2. Streptococcus salivarius and S. therrnophilus strains ...
... respectively. These data are in good agreement with the previous reports of about 38 to 42 mol % G + C within these species (Deibel & Seeley, 1974; Kilpper-Balz et al., 1982). The results of DNA-DNA hybridization experiments are shown in Table 2. Streptococcus salivarius and S. therrnophilus strains ...
Gluconacetobacter entanii sp. nov., isolated from submerged high
... vinegar fermentations in the Southern part of Germany. Four strains (LTH 4560T, LTH 4341, LTH 4551 and LTH 4637) were characterized in more detail and it was revealed that they have in common certain properties such as requirement of acetic acid, ethanol and glucose for growth, and no overoxidation ...
... vinegar fermentations in the Southern part of Germany. Four strains (LTH 4560T, LTH 4341, LTH 4551 and LTH 4637) were characterized in more detail and it was revealed that they have in common certain properties such as requirement of acetic acid, ethanol and glucose for growth, and no overoxidation ...
DNA Base Composition, DNA-DNA Homology and Long
... respectively. These data are in good agreement with the previous reports of about 38 to 42 mol % G + C within these species (Deibel & Seeley, 1974; Kilpper-Balz et al., 1982). The results of DNA-DNA hybridization experiments are shown in Table 2. Streptococcus salivarius and S. therrnophilus strains ...
... respectively. These data are in good agreement with the previous reports of about 38 to 42 mol % G + C within these species (Deibel & Seeley, 1974; Kilpper-Balz et al., 1982). The results of DNA-DNA hybridization experiments are shown in Table 2. Streptococcus salivarius and S. therrnophilus strains ...
FEBS Letters
... suitable gene probe was developed. The deduced dxr sequence of E. coli (P45568) was therefore compared to the hypothetical Dxr-like protein sequence of the sequenced genome of Synechocystis sp. (Q55663) [25] to localize conserved regions. This allowed to design suitable oligonucleotides (dxr-for, dx ...
... suitable gene probe was developed. The deduced dxr sequence of E. coli (P45568) was therefore compared to the hypothetical Dxr-like protein sequence of the sequenced genome of Synechocystis sp. (Q55663) [25] to localize conserved regions. This allowed to design suitable oligonucleotides (dxr-for, dx ...
Transcriptional analysis of the gene for glutamine synthetase II and
... Key words Streptomyces coelicolor á Nitrogen metabolism á Glutamine synthetase ...
... Key words Streptomyces coelicolor á Nitrogen metabolism á Glutamine synthetase ...
No Slide Title
... anomalies in cell-cycle regulators e.g. L.O.H. of p16, cyclin over-expression, amplification of a gene • Detection of relapse/minimal residual disease • Use in prognosis: e.g. tumors with high S phase fraction detected by flow cytometry have poorer prognosis • Use in predicting responsiveness to a p ...
... anomalies in cell-cycle regulators e.g. L.O.H. of p16, cyclin over-expression, amplification of a gene • Detection of relapse/minimal residual disease • Use in prognosis: e.g. tumors with high S phase fraction detected by flow cytometry have poorer prognosis • Use in predicting responsiveness to a p ...
No Slide Title - Oklahoma State University
... • Isolates will be identified by – Morphology – Gram-Staining – Genetics ...
... • Isolates will be identified by – Morphology – Gram-Staining – Genetics ...
transgenic animals - Worcester Polytechnic Institute
... purpose of giving it a new trait. The technology to create one has come about fairly recently in man‟s history. The first transgenic organism was created in the 1970‟s; using recombinant DNA techniques, scientists were able to take deoxyribonucleic acid (DNA) (the genetic material that makes up all ...
... purpose of giving it a new trait. The technology to create one has come about fairly recently in man‟s history. The first transgenic organism was created in the 1970‟s; using recombinant DNA techniques, scientists were able to take deoxyribonucleic acid (DNA) (the genetic material that makes up all ...
The KIebsieIIa pneumoniae cytochrome bd
... cytochrome bd’ has been extensive. Subunit I (cytochrome b5,&is encoded by cydA, and subunit I1 (which contains haem b,,, and haem d ) is encoded by cydB (Green et al., 1988). The cydAB genes are co-transcribed from a promoter upstream of cydA and are separated by 18 bp (Fang & Gennis, 1993). In sub ...
... cytochrome bd’ has been extensive. Subunit I (cytochrome b5,&is encoded by cydA, and subunit I1 (which contains haem b,,, and haem d ) is encoded by cydB (Green et al., 1988). The cydAB genes are co-transcribed from a promoter upstream of cydA and are separated by 18 bp (Fang & Gennis, 1993). In sub ...
TAP Express® Kit
... This step generates the TAP Primary® fragment, a DNA fragment that contains the gene-ofinterest with the added 5’- and 3’-TAP Ends sequences. These 5’- and 3’-TAP Ends sequences are necessary for adding the TAP Express® promoter and terminator fragments in the second PCR step. The conditions recomme ...
... This step generates the TAP Primary® fragment, a DNA fragment that contains the gene-ofinterest with the added 5’- and 3’-TAP Ends sequences. These 5’- and 3’-TAP Ends sequences are necessary for adding the TAP Express® promoter and terminator fragments in the second PCR step. The conditions recomme ...
Microbial Ecology: Where are we now?
... techniques capable of large scale multiplexing are required. Even though as compared to conventional sequencing technologies, the cost of large scale sequencing has drastically reduced (Stein 2010), it is still very expensive for WGS of complex organisms, such as humans. For this reason, emphasis is ...
... techniques capable of large scale multiplexing are required. Even though as compared to conventional sequencing technologies, the cost of large scale sequencing has drastically reduced (Stein 2010), it is still very expensive for WGS of complex organisms, such as humans. For this reason, emphasis is ...
(base) sequence of the genome might reflect biological information
... were the same due to the double helix structure of DNA. However, in this case, this coincidence of base numbers occurred in the genome, so it had nothing to do with the double helix structure. Therefore, the coincidence of base numbers occurred when the base sequence size was very large even in a si ...
... were the same due to the double helix structure of DNA. However, in this case, this coincidence of base numbers occurred in the genome, so it had nothing to do with the double helix structure. Therefore, the coincidence of base numbers occurred when the base sequence size was very large even in a si ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... Restriction enzymes applications Restriction enzymes have diversity of applications in the field of science. Gene cloning where the gene of interest (from a large genome) and the vector (plasmid) are cut with the same restriction enzyme and followed by ligation and then cloning in a bacterial cell t ...
... Restriction enzymes applications Restriction enzymes have diversity of applications in the field of science. Gene cloning where the gene of interest (from a large genome) and the vector (plasmid) are cut with the same restriction enzyme and followed by ligation and then cloning in a bacterial cell t ...
Application to Modify an Approved Exempt or Notifiable Low Risk
... This compilation was prepared on 21 September 2012 taking into account amendments up to SLI 2011 No. ...
... This compilation was prepared on 21 September 2012 taking into account amendments up to SLI 2011 No. ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... average methylation levels, more variation between subjects, and higher heritability compared to promoter regions in adipose tissue 34. To summarize, there is great interest in unraveling the factors that contribute to variation in DNA methylation between persons, but most previous twin studies of D ...
... average methylation levels, more variation between subjects, and higher heritability compared to promoter regions in adipose tissue 34. To summarize, there is great interest in unraveling the factors that contribute to variation in DNA methylation between persons, but most previous twin studies of D ...
PPT - Altogen Biosystems
... The NCI-H1299 line originates from the lung cells of a 43-year old Caucasian male patient suffering from non-small cell lung cancer. The patient received radiation prior to cell line establishment. The cell line is specifically derived from a lymph node metastatic site. The NCI-H1299 cell line displ ...
... The NCI-H1299 line originates from the lung cells of a 43-year old Caucasian male patient suffering from non-small cell lung cancer. The patient received radiation prior to cell line establishment. The cell line is specifically derived from a lymph node metastatic site. The NCI-H1299 cell line displ ...
In the near future, EU thresholds for adventitious
... GM DNA (both haploid genomes GM) will also be 100% GM seeds. However, for heterozygous GM seeds and seeds with more complex genomes (e.g. maize) results will be inconsistent using different units. Figure 1 shows how these inconsistencies can arise in a simple system. In practice, more complicated mi ...
... GM DNA (both haploid genomes GM) will also be 100% GM seeds. However, for heterozygous GM seeds and seeds with more complex genomes (e.g. maize) results will be inconsistent using different units. Figure 1 shows how these inconsistencies can arise in a simple system. In practice, more complicated mi ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... the host cell. Under some conditions, persistent infection is noted, both clinically and in tissue culture, although the mechanism by which this occurs is unknown (4). Several gene products that are temporally regulated and associated with RB:EB differentiation have been characterized. We have previ ...
... the host cell. Under some conditions, persistent infection is noted, both clinically and in tissue culture, although the mechanism by which this occurs is unknown (4). Several gene products that are temporally regulated and associated with RB:EB differentiation have been characterized. We have previ ...
Molecular Characterization of a Hamster Oviduct
... BabOGP cDNA probe [20]. The BabOGP cDNA was partially amplified by the polymerase chain reaction (PCR) in the presence of DIG-11-dUTP. Based on the DNA sequence data described by Donnelly et al. [20], two oligonucleotides (a part of the sense or anti-sense sequence of a baboon oviduct-specific glyco ...
... BabOGP cDNA probe [20]. The BabOGP cDNA was partially amplified by the polymerase chain reaction (PCR) in the presence of DIG-11-dUTP. Based on the DNA sequence data described by Donnelly et al. [20], two oligonucleotides (a part of the sense or anti-sense sequence of a baboon oviduct-specific glyco ...
GENtle, a free multi-purpose molecular biology tool
... molecular biology, especially biochemistry and genetics. Once a new domain restricted to a few experts, applications of bioinformatics today have penetrated into the daily routine of every work group in the field of molecular biology. Searching in online sequence databases have become a vital tool a ...
... molecular biology, especially biochemistry and genetics. Once a new domain restricted to a few experts, applications of bioinformatics today have penetrated into the daily routine of every work group in the field of molecular biology. Searching in online sequence databases have become a vital tool a ...
Sequence requirements for function of the
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
Practice exam 3 key
... (3 pts) Leaves single-stranded overhangs (or 'sticky ends') (1 pt); these ends are complementary (will hybridize) (1 pt); can be used to join 2 DNA fragments cut with EcoRI (with the same restriction enzyme) (1 pt). ...
... (3 pts) Leaves single-stranded overhangs (or 'sticky ends') (1 pt); these ends are complementary (will hybridize) (1 pt); can be used to join 2 DNA fragments cut with EcoRI (with the same restriction enzyme) (1 pt). ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.