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Inhibition of RNA Synthesis by Anthracycline Analogs
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
mMESSAGE mMACHINE® Kit User Guide
... to be transcribed. Circular plasmid templates will generate extremely long, heterogeneous RNA transcripts because RNA polymerases are very processive. It is generally worthwhile to examine the linearized template DNA on a gel to confirm that cleavage is complete. Since initiation of transcription is ...
... to be transcribed. Circular plasmid templates will generate extremely long, heterogeneous RNA transcripts because RNA polymerases are very processive. It is generally worthwhile to examine the linearized template DNA on a gel to confirm that cleavage is complete. Since initiation of transcription is ...
Nucleic Acids - Farmasi Unand
... suggested that the L-form may be transported into the cell by means of a L -phenylalanine active transport system. ...
... suggested that the L-form may be transported into the cell by means of a L -phenylalanine active transport system. ...
2008 HSC Notes from the Marking Centre Biology
... • look at the structure of the whole question and note that in some questions the parts follow from each other (ie responses in part (a) lead to the required response in part (b)). In Section II, the option question is divided into a number of parts. Candidates should clearly label each part of the ...
... • look at the structure of the whole question and note that in some questions the parts follow from each other (ie responses in part (a) lead to the required response in part (b)). In Section II, the option question is divided into a number of parts. Candidates should clearly label each part of the ...
2004-011: Draft Annex to ISPM 27:2006 – Xanthomonas citri subsp
... multi-window microscope slide, allowed to air dry and then gently heat-fixed over a flame. Separate slides are set up for each test bacterium, and also for positive and negative controls as are used for enzyme-linked immunosorbent assay (ELISA). Commercially available antiserum is diluted with PBS ( ...
... multi-window microscope slide, allowed to air dry and then gently heat-fixed over a flame. Separate slides are set up for each test bacterium, and also for positive and negative controls as are used for enzyme-linked immunosorbent assay (ELISA). Commercially available antiserum is diluted with PBS ( ...
DNA cytosine methylation in plant development
... opinion that cytosine methylation is indispensible for normal plant development has been reinforced by using single or combinations of diverse loss-of-function mutants for DNA methyltransferases, DNA glycosylases, components involved in siRNA biogenesis and chromatin remodeling factors. Patterns of ...
... opinion that cytosine methylation is indispensible for normal plant development has been reinforced by using single or combinations of diverse loss-of-function mutants for DNA methyltransferases, DNA glycosylases, components involved in siRNA biogenesis and chromatin remodeling factors. Patterns of ...
Molecular Identification of Nematodes Manual
... PCR run.) Normally, one minute at 94oC is sufficient. *Annealing involves the matching of the two primers to their specific “binding sites.” We use this particular form of PCR where two primers are used in the reaction. They were specifically designed from a DNA template sequence (previously obtaine ...
... PCR run.) Normally, one minute at 94oC is sufficient. *Annealing involves the matching of the two primers to their specific “binding sites.” We use this particular form of PCR where two primers are used in the reaction. They were specifically designed from a DNA template sequence (previously obtaine ...
Polymerase Chain Reaction In Ophthalmology
... • PCR cannot detect the organism for which primers have not been provided. So a narrow and well defined differential diagnosis is required for PCR to be effectively useful. PCR and Koch's postulates 6, 7 Koch's postulates include isolation of suspected pathogen from all cases of a disease, successfu ...
... • PCR cannot detect the organism for which primers have not been provided. So a narrow and well defined differential diagnosis is required for PCR to be effectively useful. PCR and Koch's postulates 6, 7 Koch's postulates include isolation of suspected pathogen from all cases of a disease, successfu ...
Resolving Individuals Contributing Trace Amounts of DNA to Highly
... arrays typically have 3 to 4 probes for the A allele and B allele respectively, whereas Illumina arrays have a random number of probes averaging approximately 18 probes per allele. With 500,000+ SNPs, there are millions of probes (or features) on a SNP genotyping array. One should note that there ar ...
... arrays typically have 3 to 4 probes for the A allele and B allele respectively, whereas Illumina arrays have a random number of probes averaging approximately 18 probes per allele. With 500,000+ SNPs, there are millions of probes (or features) on a SNP genotyping array. One should note that there ar ...
rec-mediated recombinational hot spot activity in bacteriophage
... I n order to survey die entire A chromosome in a single experiment for a possible recombinational hot spot, crosses are performed which in this manuscript are termed “hot spot survey crosses.” These crosses are carried out using conditions which block nearly all DNA synthesis (MCMILINand Russo 1972) ...
... I n order to survey die entire A chromosome in a single experiment for a possible recombinational hot spot, crosses are performed which in this manuscript are termed “hot spot survey crosses.” These crosses are carried out using conditions which block nearly all DNA synthesis (MCMILINand Russo 1972) ...
Maintenance of genomic integrity by p53: complementary
... the control of genomic integrity in its non-induced state, i.e., in the absence of signals indicating DNA damage or other potentially harmful conditions, so far has not yet been considered at all. In contrast, the general assumption is that a non-activated p53 is a non-functional one (Hupp and Lane, ...
... the control of genomic integrity in its non-induced state, i.e., in the absence of signals indicating DNA damage or other potentially harmful conditions, so far has not yet been considered at all. In contrast, the general assumption is that a non-activated p53 is a non-functional one (Hupp and Lane, ...
Mitochondrial DNA (mtDNA) Biogenesis: Visualization and Duel
... PBS. In some cases, cells were incubated with Image-iT FX Signal Enhancer (Invitrogen; Eugene, OR) for 30 min at room temperature to reduce background staining of Alexa Fluor secondary antibodies. For standard immunofluorescence, cells were blocked in PBS containing 0.1% Triton X-100 and 5% goat ser ...
... PBS. In some cases, cells were incubated with Image-iT FX Signal Enhancer (Invitrogen; Eugene, OR) for 30 min at room temperature to reduce background staining of Alexa Fluor secondary antibodies. For standard immunofluorescence, cells were blocked in PBS containing 0.1% Triton X-100 and 5% goat ser ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
... sequence analysis of exon VI11 showed the presence of two bands at the same migration distance, corresponding to Lys (AAC + AAA). amino acid substitution y 337 Asn The purified double-stranded PCR-derived fragment was cloned and subjected to sequence analysis. Normal and mutated alleles of fibrinoge ...
... sequence analysis of exon VI11 showed the presence of two bands at the same migration distance, corresponding to Lys (AAC + AAA). amino acid substitution y 337 Asn The purified double-stranded PCR-derived fragment was cloned and subjected to sequence analysis. Normal and mutated alleles of fibrinoge ...
transposon
... but we can distinguish three different types of mechanism by which a transposon moves: replicative, nonreplicative, and conservative ...
... but we can distinguish three different types of mechanism by which a transposon moves: replicative, nonreplicative, and conservative ...
A Novel CpG Island Set Identifies Tissue-Specific
... resulting library was sequenced to define a novel human blood CGI set that includes many that are not detected by current algorithms. Approximately half of CGIs were associated with annotated gene transcription start sites, the remainder being intra- or intergenic. Using an array representing over 1 ...
... resulting library was sequenced to define a novel human blood CGI set that includes many that are not detected by current algorithms. Approximately half of CGIs were associated with annotated gene transcription start sites, the remainder being intra- or intergenic. Using an array representing over 1 ...
Taxonomic characterization of Ochrobactrum sp. isolates from soil
... scales of taxonomic resolution and for an evaluation of exogenous influences on microbial diversity in soil. It is, however, an essential prerequisite for such studies to unambiguously identify and characterize the investigated strains at and below the species level. The objective of the present stu ...
... scales of taxonomic resolution and for an evaluation of exogenous influences on microbial diversity in soil. It is, however, an essential prerequisite for such studies to unambiguously identify and characterize the investigated strains at and below the species level. The objective of the present stu ...
Molecular genetics of the extracellular lipase of
... lipase gene. Vectors: pBluescript SK II/pUC19Ps. Line drawings of inserts are to scale. Restriction sites are indicated by capital letters B : BurnHI; E, EcoRI; S, Sun; X, XhoI. Genes IipA and IipH are included as boxes; arrows within indicate direction of transcription. ...
... lipase gene. Vectors: pBluescript SK II/pUC19Ps. Line drawings of inserts are to scale. Restriction sites are indicated by capital letters B : BurnHI; E, EcoRI; S, Sun; X, XhoI. Genes IipA and IipH are included as boxes; arrows within indicate direction of transcription. ...
Pet_SUMO manual
... ProBond™ or Ni-NTA resins to purify your recombinant fusion protein. You may also use ProBond™ or Ni-NTA resins to remove the SUMO fusion protein and SUMO Protease from the cleavage reaction once you have generated native protein. Ordering information for these products is provided below. ...
... ProBond™ or Ni-NTA resins to purify your recombinant fusion protein. You may also use ProBond™ or Ni-NTA resins to remove the SUMO fusion protein and SUMO Protease from the cleavage reaction once you have generated native protein. Ordering information for these products is provided below. ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... had isolated resembled P22Tc-10. Thus, we wondered if P22Tc-10 were a special case. The electron microscopy of P22Tc-10 DNA seemed to confirm this notion: TYE,CHANand BOTSTEIN(1974) found that the ends of the Tc-10 insertion contained an inverted repeat which was apparently identical to the inverted ...
... had isolated resembled P22Tc-10. Thus, we wondered if P22Tc-10 were a special case. The electron microscopy of P22Tc-10 DNA seemed to confirm this notion: TYE,CHANand BOTSTEIN(1974) found that the ends of the Tc-10 insertion contained an inverted repeat which was apparently identical to the inverted ...
... smaller and interacts with the non-polar core via van der Waals by a lesser extent. (+5 pts) 10. (12 pts) Allosteric effects play a predominate role in many aspects of biochemistry. Briefly define the term allosteric, and discuss the role of allosteric effects in any one of the following. a) Oxygen ...
Glycolysis Lecture
... no reason to suppose that Biochemistry is intrinsically uninteresting, difficult to understand or an obstacle to be overcome during your progress to a professional qualifications. In the contrary you can enjoy its study if you remember the simple biological principals that the body is formed from or ...
... no reason to suppose that Biochemistry is intrinsically uninteresting, difficult to understand or an obstacle to be overcome during your progress to a professional qualifications. In the contrary you can enjoy its study if you remember the simple biological principals that the body is formed from or ...
New peptide and gene coding for same
... Figure 7 represents a result of electrophoresis showing expression of a 7-hANP gene. Figure 8 represents a construction process of plasmid pS223-3 from plasmid pS83-3 and a DNA fragment containing a base sequence coding for amino acid sequence of 7-hANP, and a construction process of plasmid pS225-3 ...
... Figure 7 represents a result of electrophoresis showing expression of a 7-hANP gene. Figure 8 represents a construction process of plasmid pS223-3 from plasmid pS83-3 and a DNA fragment containing a base sequence coding for amino acid sequence of 7-hANP, and a construction process of plasmid pS225-3 ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.