A Pneumocystis carinii multi-gene family with
... was carried out using both probes, and secondary and tertiary screens were carried out using only the 9/4r product. The number of positive clones when screening the cDNA library with the two probes appeared to be relatively high when compared to the number obtained using a single copy gene. Four rec ...
... was carried out using both probes, and secondary and tertiary screens were carried out using only the 9/4r product. The number of positive clones when screening the cDNA library with the two probes appeared to be relatively high when compared to the number obtained using a single copy gene. Four rec ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... diaminobutane moiety into a fully extended conformation and produces a single distance (approximately 6.3 A) between the secondary amino groups (data not shown). The aminopropyl groups attached to the secondary amino groups assume a variety of conformations, however, and there are a variety of dista ...
... diaminobutane moiety into a fully extended conformation and produces a single distance (approximately 6.3 A) between the secondary amino groups (data not shown). The aminopropyl groups attached to the secondary amino groups assume a variety of conformations, however, and there are a variety of dista ...
Next-Generation Sequencing: an overview of technologies and
... with sequencing adapters – Well suited for 454 and bench top sequencers – Deep sequencing for detection of somatic mutations – 16S Sequencing for microbial diversity ...
... with sequencing adapters – Well suited for 454 and bench top sequencers – Deep sequencing for detection of somatic mutations – 16S Sequencing for microbial diversity ...
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic
... plasmids includes new shuttle vectors that can be used with histidine and adenine auxotrophic laboratory yeast strains carrying mutations in the genes HIS2 and ADE1, respectively. Our pRSII plasmids also include updated versions of commonly used pRS plasmids from which common restriction sites that ...
... plasmids includes new shuttle vectors that can be used with histidine and adenine auxotrophic laboratory yeast strains carrying mutations in the genes HIS2 and ADE1, respectively. Our pRSII plasmids also include updated versions of commonly used pRS plasmids from which common restriction sites that ...
Detection of GM Papaya Event 55-1 in Fresh
... the more than 130 GMOs developed in other countries have been approved in Korea [Kim et al., 2010]. Therefore, it is necessary to develop effective GMO detection methods to monitor and identify approved as well as unapproved GMOs. In this study, we designed a set of primers to amplify an endogenous ...
... the more than 130 GMOs developed in other countries have been approved in Korea [Kim et al., 2010]. Therefore, it is necessary to develop effective GMO detection methods to monitor and identify approved as well as unapproved GMOs. In this study, we designed a set of primers to amplify an endogenous ...
World Index of BioMolecular Visualization Resources
... Cross-indexing terms: antibody; antibodies; multiple sequence alignment; sequence variability; homology modelling; immunoglobulin; domains; color-coded multiple sequence alignment. This site contains extensive tutorials and lectures on antibody structure coupled with analyses of sequence variability ...
... Cross-indexing terms: antibody; antibodies; multiple sequence alignment; sequence variability; homology modelling; immunoglobulin; domains; color-coded multiple sequence alignment. This site contains extensive tutorials and lectures on antibody structure coupled with analyses of sequence variability ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... fragments toward the cathode (positive pole) • Larger fragments move more slowly through the agarose gel matrix than small fragments and hence the fragments are size fractionated • The resolution of separation depends on the type and concentration of gel and the length of the run: ‚mini‘ gels are fa ...
... fragments toward the cathode (positive pole) • Larger fragments move more slowly through the agarose gel matrix than small fragments and hence the fragments are size fractionated • The resolution of separation depends on the type and concentration of gel and the length of the run: ‚mini‘ gels are fa ...
Biology Final Exam Review Questions Answer Section SHORT
... Because the offspring of asexual reproduction are genetically identical to parents, they have the characteristics that help them survive in the conditions in which the parent cells survived. They might not have characteristics to survive should the conditions change. PTS: 1 DIF: L3 REF: p. 278 OBJ: ...
... Because the offspring of asexual reproduction are genetically identical to parents, they have the characteristics that help them survive in the conditions in which the parent cells survived. They might not have characteristics to survive should the conditions change. PTS: 1 DIF: L3 REF: p. 278 OBJ: ...
Selection for TnlO Tet Repressor Binding to tet Operator
... We have constructed a genetic assay which selects positively for a functional interaction between Tet repressor and its cognate operator in Escherichia coli. In this strain Tet repressor blocks expression of lacl and lacZ. This leads to derepression of a lacPO controlled galK gene. The strain can be ...
... We have constructed a genetic assay which selects positively for a functional interaction between Tet repressor and its cognate operator in Escherichia coli. In this strain Tet repressor blocks expression of lacl and lacZ. This leads to derepression of a lacPO controlled galK gene. The strain can be ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... reactions are singleplex. Please note that the “multiplex” in the GMO kit is slightly different than the “multiplexing” described above. In the GMO Investigator kit, primer pairs are mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I ...
... reactions are singleplex. Please note that the “multiplex” in the GMO kit is slightly different than the “multiplexing” described above. In the GMO Investigator kit, primer pairs are mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I ...
Plant Molecular Biology
... investigated for the presence of additional nodulation genes. Complementation experiments indicated that the D N A region to the left (3' side) of nodE is functionally homologous between R. leguminosarum bv. vieiae and R. leguminosarum bv. trifolii. In this D N A region, three nodulation genes were ...
... investigated for the presence of additional nodulation genes. Complementation experiments indicated that the D N A region to the left (3' side) of nodE is functionally homologous between R. leguminosarum bv. vieiae and R. leguminosarum bv. trifolii. In this D N A region, three nodulation genes were ...
Sequencing breakthroughs for genomic ecology and
... and to identify unculturable microorganisms from their DNA sequence (reviewed by Handelsman 2004) have all allowed great advances in their respective fields and in many cases had much broader impacts on society. However, all these techniques are based on the analysis of a tiny fragment of an organis ...
... and to identify unculturable microorganisms from their DNA sequence (reviewed by Handelsman 2004) have all allowed great advances in their respective fields and in many cases had much broader impacts on society. However, all these techniques are based on the analysis of a tiny fragment of an organis ...
Base-pair neutral homozygotes can be discriminated by calibrated
... This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is proper ...
... This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is proper ...
Nucleic acid vaccines against rickettsial diseases and methods of use
... This application is a continuation-in-part of Ser. No. 09/337,827, ?led Jun. 22, 1999 noW abandoned; Which is a divisional of Ser. No. 08/953,326, ?led Oct. 17, 1997, now US. Pat. No. 6,251,872; Which is a continuation-in-part of ...
... This application is a continuation-in-part of Ser. No. 09/337,827, ?led Jun. 22, 1999 noW abandoned; Which is a divisional of Ser. No. 08/953,326, ?led Oct. 17, 1997, now US. Pat. No. 6,251,872; Which is a continuation-in-part of ...
Diagnostic protocol for
... centrifuged at 5000 g for 5 min, 450 µl of the supernatant is transferred and 450 µl isopropanol is added. The suspension is mixed gently and left at room temperature for 1 h. Precipitation can be improved by the use of Pellet Paint Coprecipitant (Cubero et al., 2001). The suspension is centrifuged ...
... centrifuged at 5000 g for 5 min, 450 µl of the supernatant is transferred and 450 µl isopropanol is added. The suspension is mixed gently and left at room temperature for 1 h. Precipitation can be improved by the use of Pellet Paint Coprecipitant (Cubero et al., 2001). The suspension is centrifuged ...
Foundations of Biology
... Pharming is the use of recombinant domestic animals to produce proteins and other bioactive compounds One of the most common strategies in pharming involves engineering mammals to produce desired proteins in their milk This strategy was first used to produce t-PA in the milk of goats It is not coinc ...
... Pharming is the use of recombinant domestic animals to produce proteins and other bioactive compounds One of the most common strategies in pharming involves engineering mammals to produce desired proteins in their milk This strategy was first used to produce t-PA in the milk of goats It is not coinc ...
Conjugative plasmids: vessels of the communal gene pool
... These two factors, compactness and gene clustering, mean that many conferrable traits can be transferred between discrete replicating elements (chromosomes or plasmids) by the movement of relatively small fragments of DNA. Phylogenetic analyses of complete genomes have also shown that some prokaryot ...
... These two factors, compactness and gene clustering, mean that many conferrable traits can be transferred between discrete replicating elements (chromosomes or plasmids) by the movement of relatively small fragments of DNA. Phylogenetic analyses of complete genomes have also shown that some prokaryot ...
HL7 V2.5.1 Genetic Test Result Message - HL7 Wiki
... 2.5.1. This implementation guide is modeled after the HL7 Version 2.5.1 Implementation Guide: Orders And Observations; Interoperable Laboratory Result Reporting To EHR (US Realm), Release 1 and covers the reporting of genetic test results for sequencing and genotyping based tests where identified DN ...
... 2.5.1. This implementation guide is modeled after the HL7 Version 2.5.1 Implementation Guide: Orders And Observations; Interoperable Laboratory Result Reporting To EHR (US Realm), Release 1 and covers the reporting of genetic test results for sequencing and genotyping based tests where identified DN ...
Natural Computing - Computer Science
... and categorize them as self or non-self • Innate (non-specific) immune system • Adaptive (acquired) immune system ...
... and categorize them as self or non-self • Innate (non-specific) immune system • Adaptive (acquired) immune system ...
WOTD - Brookwood High School
... two or more alleles are expressed at the same time, both are expressed in patches on the organism ...
... two or more alleles are expressed at the same time, both are expressed in patches on the organism ...
Epigenetic Regulation ofbdnfGene Transcription in the
... PCRs were in an iQ5 real-time PCR system (Bio-Rad) using the following cycling conditions: 95°C for 3 min, 40 cycles of 95°C for 15 s, and 58 – 60°C for 1 min. Detection of the fluorescent products occurred at the end of the 15 s temperature step. For a melting curve analysis, PCR products were melt ...
... PCRs were in an iQ5 real-time PCR system (Bio-Rad) using the following cycling conditions: 95°C for 3 min, 40 cycles of 95°C for 15 s, and 58 – 60°C for 1 min. Detection of the fluorescent products occurred at the end of the 15 s temperature step. For a melting curve analysis, PCR products were melt ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.