... is inhibited. As the ncRNA is not produced maternally, most genes within the domain are free to be expressed from that chromosome (Figure 1). Recent experiments have strongly suggested that the silencing mechanism of Kcnq1ot1 RNA involves a spreading activity in cis, recruitment of Polycomb group pr ...
Human Growth Hormone
... The Hayflick Limit is named for the scientist who discovered it. Cells may normally only undergo a finite number of divisions. After each division the protective piece of DNA at the end of each chromosome called the telomere gets shorter, and once the telomere's length is reduced to a certain size, ...
... The Hayflick Limit is named for the scientist who discovered it. Cells may normally only undergo a finite number of divisions. After each division the protective piece of DNA at the end of each chromosome called the telomere gets shorter, and once the telomere's length is reduced to a certain size, ...
Chpt10_TxnRNAPol.doc
... In living cells, RNA polymerases usually start transcription at the beginning of genes. The segment of DNA required for specific initiation of transcription by RNA polymerase is called a promoter; it is commonly adjacent to the 5’ end of a gene. (Promoters will be covered in more detail in the next ...
... In living cells, RNA polymerases usually start transcription at the beginning of genes. The segment of DNA required for specific initiation of transcription by RNA polymerase is called a promoter; it is commonly adjacent to the 5’ end of a gene. (Promoters will be covered in more detail in the next ...
Application Note 129 – Eppendorf MixMate
... yesterday they were still using 0.5 ml PCR tubes, now they are using 96- or 384-well PCR plates. Whereas only a few years ago, 10 ml tubes were still being used for bacterial cultures, now deepwell plates are the norm. However, these new formats and their special geometries, together with the smalle ...
... yesterday they were still using 0.5 ml PCR tubes, now they are using 96- or 384-well PCR plates. Whereas only a few years ago, 10 ml tubes were still being used for bacterial cultures, now deepwell plates are the norm. However, these new formats and their special geometries, together with the smalle ...
Universal chloroplast integration and expression vectors
... The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants ...
... The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants ...
Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
Snorks Lab File
... DNA TO PROTEINS Demonstrate how the genetic information in DNA molecules provides instructions for assembling protein molecules and that this is virtually the same mechanism for all life forms. ...
... DNA TO PROTEINS Demonstrate how the genetic information in DNA molecules provides instructions for assembling protein molecules and that this is virtually the same mechanism for all life forms. ...
PCR Primer Design
... Primer secondary structures: Presence of the primer secondary structures adversely affect primer template annealing and thus the amplification. They greatly reduce the availability of primers to the reaction. ...
... Primer secondary structures: Presence of the primer secondary structures adversely affect primer template annealing and thus the amplification. They greatly reduce the availability of primers to the reaction. ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... use of radiation for cancer treatment, but it is also the basis for the harmful side-effects of radiation exposure. It has also been shown in cells that double-strand breaks can be repaired in an error-free and error-prone fashion, and that error-prone repair often leads to mutations associated with ...
... use of radiation for cancer treatment, but it is also the basis for the harmful side-effects of radiation exposure. It has also been shown in cells that double-strand breaks can be repaired in an error-free and error-prone fashion, and that error-prone repair often leads to mutations associated with ...
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
... group, 2004). According to these recommendations, ‘a phytoplasma can be described as a novel ‘Candidatus Phytoplasma’ species if its 16S rDNA sequence has <97?5 % similarity to that of any previously described ‘Candidatus Phytoplasma’ species’. In cases where phytoplasmas share ¢97?5 % 16S rDNA sequ ...
... group, 2004). According to these recommendations, ‘a phytoplasma can be described as a novel ‘Candidatus Phytoplasma’ species if its 16S rDNA sequence has <97?5 % similarity to that of any previously described ‘Candidatus Phytoplasma’ species’. In cases where phytoplasmas share ¢97?5 % 16S rDNA sequ ...
COMPUTATIONAL MODELING OF CHARGE TRANSFER IN NUCLEOBASE-AROMATIC AMINO ACID COMPLEXES Cristina BUTCHOSA ROBLES
... states are also called electron ”holes”. Electron holes can migrate long distances through the nucleobases stack, due to conductivity properties of DNA. Finally, the cationic charge could be trapped and most probably a mutagenic lesion will be initiated. However, if DNA interacts with a protein or p ...
... states are also called electron ”holes”. Electron holes can migrate long distances through the nucleobases stack, due to conductivity properties of DNA. Finally, the cationic charge could be trapped and most probably a mutagenic lesion will be initiated. However, if DNA interacts with a protein or p ...
Short-Chain-Length Polyhydroxyalkanoates: Synthesis in
... 3-, 4-, 5-, or 6-carbon position with various side groups have been found to be incorporated into PHAs [55]. Since PHAs exhibit biodegradable/biocompatible plastic and elastomeric properties, depending on the monomer constituents, their commercial applications have been extensively studied. Accordin ...
... 3-, 4-, 5-, or 6-carbon position with various side groups have been found to be incorporated into PHAs [55]. Since PHAs exhibit biodegradable/biocompatible plastic and elastomeric properties, depending on the monomer constituents, their commercial applications have been extensively studied. Accordin ...
Genetic tools for manipulating Acinetobacter baumannii genome: an
... as a temperature-sensitive replicon. The vector must also carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer me ...
... as a temperature-sensitive replicon. The vector must also carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer me ...
Characterization of the chimeric seven
... Recently, many PR-like genes were found in non-marine environments. The goal of this study is to explore the function of rhodopsins that exist only as partial proteoopsin genes using chimeras with marine green PR (GPR). We isolated nine partial genes of PR homologues using polymerase chain reaction ...
... Recently, many PR-like genes were found in non-marine environments. The goal of this study is to explore the function of rhodopsins that exist only as partial proteoopsin genes using chimeras with marine green PR (GPR). We isolated nine partial genes of PR homologues using polymerase chain reaction ...
pdf
... In living cells, RNA polymerases usually start transcription at the beginning of genes. The segment of DNA required for specific initiation of transcription by RNA polymerase is called a promoter; it is commonly adjacent to the 5’ end of a gene. (Promoters will be covered in more detail in the next ...
... In living cells, RNA polymerases usually start transcription at the beginning of genes. The segment of DNA required for specific initiation of transcription by RNA polymerase is called a promoter; it is commonly adjacent to the 5’ end of a gene. (Promoters will be covered in more detail in the next ...
Single-Nucleotide Polymorphism Mapping
... SNP, this may be an increase in cut or in uncut DNA. The ratio is the relevant parameter, not the absolute amount of product. When using eight SNPs per chromosome, a mutation will show tight linkage to at least one SNP and noticeable linkage to adjacent SNPs. Weak linkage to one SNP, but not to adja ...
... SNP, this may be an increase in cut or in uncut DNA. The ratio is the relevant parameter, not the absolute amount of product. When using eight SNPs per chromosome, a mutation will show tight linkage to at least one SNP and noticeable linkage to adjacent SNPs. Weak linkage to one SNP, but not to adja ...
MycoplasMa Quality control
... Because mycoplasma can affect the phenotypic and functional characteristics of cells in vitro, the implementation of early detection methods that are rapid and sensitive is an important step towards preventing the deleterious and costly effects of mycoplasma on research and development projects. The ...
... Because mycoplasma can affect the phenotypic and functional characteristics of cells in vitro, the implementation of early detection methods that are rapid and sensitive is an important step towards preventing the deleterious and costly effects of mycoplasma on research and development projects. The ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... The food industry is interested in the production of esters for use as flavouring compounds; for example, esters are responsible for the fruity character of fermented alcoholic beverages such as beer and wine. The ability to create large quantities of an ester or to genetically alter a host to produ ...
... The food industry is interested in the production of esters for use as flavouring compounds; for example, esters are responsible for the fruity character of fermented alcoholic beverages such as beer and wine. The ability to create large quantities of an ester or to genetically alter a host to produ ...
Identification and isolation of active N2O reducers in rice paddy soil
... the occurrence of N2O reduction in rice paddy fields; however, identity of the N2O reducers is largely unknown. In this study, we employed both culture-dependent and culture-independent approaches to identify N2O reducers in rice paddy soil. In a soil microcosm, N2O and succinate were added as the e ...
... the occurrence of N2O reduction in rice paddy fields; however, identity of the N2O reducers is largely unknown. In this study, we employed both culture-dependent and culture-independent approaches to identify N2O reducers in rice paddy soil. In a soil microcosm, N2O and succinate were added as the e ...
One-stop polymerase chain reaction (PCR): An improved PCR
... Figure 2. Efficiency and effect of one-stop PCR on nucleic acid amplification. (A) One-stop PCR is comparable to commercially available PCR kits. A 0.4 kb fragment of human β-actin mRNA was amplified with gradient template amount at dilutions of 1/1, 1/10, 1/100. All the PCR products were separated ...
... Figure 2. Efficiency and effect of one-stop PCR on nucleic acid amplification. (A) One-stop PCR is comparable to commercially available PCR kits. A 0.4 kb fragment of human β-actin mRNA was amplified with gradient template amount at dilutions of 1/1, 1/10, 1/100. All the PCR products were separated ...
using polymerase chain reaction for detection of enterotoxigenic
... cholera toxin, this result indicated that sequence of gene was at location 1-22 of target gene with length 564 bp of ctxA gene. Sequence of primers and length of target gene were consistent with the previous studies of Olsvik et al., (1993) and the name of this primer is ctxA’. Study specificity of ...
... cholera toxin, this result indicated that sequence of gene was at location 1-22 of target gene with length 564 bp of ctxA gene. Sequence of primers and length of target gene were consistent with the previous studies of Olsvik et al., (1993) and the name of this primer is ctxA’. Study specificity of ...
The Rad50 Signature Motif: Essential to ATP Binding and
... for the ATP-induced DNA unwinding catalyzed by the M/R/N complex.16 In this study, we further investigate the role of the signature motif in Rad50 function by crystallizing a form of the Rad50cd containing a serine to arginine mutation in the signature motif (Rad50cdS793R). This S793R in the ABC ATP ...
... for the ATP-induced DNA unwinding catalyzed by the M/R/N complex.16 In this study, we further investigate the role of the signature motif in Rad50 function by crystallizing a form of the Rad50cd containing a serine to arginine mutation in the signature motif (Rad50cdS793R). This S793R in the ABC ATP ...
International Plant Protection Convention Draft annex to ISPM 27
... control or from positive samples. If required, the positive control used in the lab should be sequenced so that this sequence can be readily compared to sequence obtained from PCR amplicons of the correct size. Alternatively, synthetic positive controls can be made with a known sequence which again ...
... control or from positive samples. If required, the positive control used in the lab should be sequenced so that this sequence can be readily compared to sequence obtained from PCR amplicons of the correct size. Alternatively, synthetic positive controls can be made with a known sequence which again ...
hag expression in Bacillus subtilis is both negatively
... ml21 and incubation continued for 2.5 h. Cells were harvested by centrifugation and the pellet was washed with 1 ml buffer containing 25 mM Tris/HCl, pH 7.4. The pellet was resuspended in 500 ml buffer containing 25 mM Tris/HCl, pH 7.4, and cells were lysed by lysozyme treatment at 30 uC for 15 min, ...
... ml21 and incubation continued for 2.5 h. Cells were harvested by centrifugation and the pellet was washed with 1 ml buffer containing 25 mM Tris/HCl, pH 7.4. The pellet was resuspended in 500 ml buffer containing 25 mM Tris/HCl, pH 7.4, and cells were lysed by lysozyme treatment at 30 uC for 15 min, ...
Introduction - Stellenbosch University
... Pevsner J. Bioinformatics and Functional Genomics 3rd Edition Wiley-Blackwell 2015 Bioinformatics, Stellenbosch University ...
... Pevsner J. Bioinformatics and Functional Genomics 3rd Edition Wiley-Blackwell 2015 Bioinformatics, Stellenbosch University ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.