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Genetic polymorphisms among C57BL/6 mouse inbred strains
Genetic polymorphisms among C57BL/6 mouse inbred strains

... examining the genotype because of the possible effects on phenotype. Thus, knockout and transgenic mice produced in C57BL/6J and C57BL/6N inbred substrains might co-exist in a given animal house and, therefore great care should be taken to accurately monitor the genotypes of mouse colonies or when a ...
TaqMan® SNP Genotyping Assays User Guide
TaqMan® SNP Genotyping Assays User Guide

... particular level of observation or actions as described below: Note: Provides information that may be of interest or help but is not critical to the use of the product. ...
2.1.databases_intro - T
2.1.databases_intro - T

... Fully-annotated (manually), non-redundant, crossreferenced, documented protein sequence database. ~100 ’000 sequences from more than 6’800 different species; 70 ’000 references (publications); 550 ’000 crossreferences (databases); ~200 Mb of annotations. ...
Genetics Practice Test
Genetics Practice Test

... 58. An organism’s gametes have ____________________ the number of chromosomes found in the organism’s body cells. 59. Crossing-over occurs during the stage of meiosis called ____________________. 60. The relative locations of each known gene can be shown on a ____________________ map. 61. In humans, ...
ontology design patterns for the formalisation of biological ontologies
ontology design patterns for the formalisation of biological ontologies

... that range from plain DNA sequences to complex protein 3D structures. All those data are annotated, including information about origin, reliability, structural interpretation, possible drug targets, etc. The first Bioinformatics resources that stored those data were built on flat files, and then in ...
Creating order from chaos: epigenome dynamics in
Creating order from chaos: epigenome dynamics in

... aspects of chromatin regulation that might be variable, or drive variation among closely ...
VAAST 2 User`s Guide
VAAST 2 User`s Guide

... annotating  genomic  features.    Although  various  versions  of  GTF  and  GFF  formats   have  been  in  use  for  many  years,  GFF3  was  introduced  by  Lincoln  Stein  to   standardize  the  various  gene  annotation  formats  to ...
a method for detecting and typing of salmonella by multiplex pcr
a method for detecting and typing of salmonella by multiplex pcr

... service should have time to check the quality of all of these products. Only traditional bacteriological methods of isolation and identification of pathogens of toxicoinfection, which is not enough in terms of increasing turnover of products, are used today. The one of the most dangerous toxicoinfec ...
Product description P018-G1 SHOX-v03 - MRC
Product description P018-G1 SHOX-v03 - MRC

... To be used in combination with a SALSA® MLPA® reagent kit, available for various number of reactions. MLPA reagent kits are either provided with FAM or Cy5.0 dye-labelled PCR primer, suitable for Applied Biosystems and Beckman capillary sequencers, respectively (see www.mlpa.com). Certificate of Ana ...
Asymmetries in Retrieval of Gene Function Information
Asymmetries in Retrieval of Gene Function Information

... of the source followed by a slash followed by an accession number and can be searched with one or both components, e.g., genbank [si], AF001892 [si], genbank/AF001892 [si]. The SI field and the Entrez sequence database links are not linked. The PubMed links to these databases are created from the re ...
Real-time PCR Handbook
Real-time PCR Handbook

... Primer design software programs, such as OligoPerfect™ designer and Primer Express® software and sequence analysis software, such as Vector NTI® Software can automatically evaluate a target sequence and design primers for it based on the criteria listed above. At a minimum, using primer design softw ...
Transvection Is Common Throughout the Drosophila
Transvection Is Common Throughout the Drosophila

... populations of neurons, we have been taking advantage of a recently developed toolset composed of transgenes in which GAL4 is driven by cis-regulatory modules (CRMs) taken from various gene loci (Pfeiffer et al. 2008). These transgenes have been inserted into the commonly used attP2 integration site ...
To on your computer
To on your computer

... Compilation of protein analysis tools@ Expasy: http://www.expasy.org/proteomics Compilation of protein analysis tools @ CBS : http://www.cbs.dtu.dk/services/ Compute PI/MW@expasy: http://web.expasy.org/compute_pi/ Find a protein sequence based on the AA composition@Expasy: http://web.expasy.org/aaco ...
NO sensing by FNR: regulation of the Escherichia coli NO
NO sensing by FNR: regulation of the Escherichia coli NO

... cluster. A minor species appearing at g = 2.15 may arise from a thiyl radical (data not shown; Kennedy et al., 1997). The EPR spectra measured at low temperature (not shown) revealed a trace amount of a [3Fe±4S]1+ cluster at g = 2.01. In a parallel set of experiments, sequential additions of NO solu ...
hhmi resource for new ap bio curriculum
hhmi resource for new ap bio curriculum

... applicable for the animations, video clips, etc… so it is easy to see how long a video resource will run if used in the classroom. AP® Biology Correlations: This column correlates with the new AP® Biology Curriculum Framework. Each Big Idea (1-4) is at the heading of a sub-column. As a teacher scans ...
The landscape of Neandertal ancestry in present
The landscape of Neandertal ancestry in present

... ancestry: on a 10 Megabase (Mb) scale on the autosomes, there are 4 windows in Europeans and 14 in East Asians with Neandertal ancestry <0.1% (Extended Data Fig. 2, SI 8). Two analyses show that these deserts are not artifacts of reduced power to detect ancestry. First, when we lower the probability ...
Attachment 2.2 Sequencing results
Attachment 2.2 Sequencing results

... Hypotheses: two hypotheses were formulated, namely: - The AHR gene is highly conserved. - There are multiple transcripts of the AHR gene in the dog Conservation: the protein coding transcripts of human, dog, mouse, rat, horse, chicken, rabbit and zebrafish were compared by blasting them in NCBI Blas ...
Additional file 1
Additional file 1

... 1、 The protein sequence of bh2b may be queried against transcription factor database of Jaspar[3] with blastp to gather information on likely DNA binding sites of bh2b, which is based on the PWMs of transcription factors that are similar to bh2b. The blastp result shows that bh2b is similar with tra ...
SALSA MLPA probemix P018-F1 SHOX - MRC
SALSA MLPA probemix P018-F1 SHOX - MRC

... Data normalisation should be performed within one experiment. Only samples purified by the same method should be compared. Confirmation of most exons deletions and amplifications can be done by e.g. Southern blots or long range PCR. Note that Coffalyser, the MLPA analysis tool developed at MRC-Holla ...
Rfam Documentation
Rfam Documentation

... secondary structure for these sequences. The ideal basis for a new family is an RNA element that: • has some known functional classification • is evolutionarily conserved • has evidence for a secondary structure In order to build a new family, we must first obtain at least one experimentally validat ...
The Parasexual Cycle in Candida albicans Provides an
The Parasexual Cycle in Candida albicans Provides an

... been observed. Instead, tetraploid products of mating can be induced to undergo efficient, random chromosome loss, often producing strains that are diploid, or close to diploid, in ploidy. Using SNP and comparative genome hybridization arrays we have now analyzed the genotypes of products from the C ...
Quantitative analysis of SMN1 and SMN2 genes based on DHPLC
Quantitative analysis of SMN1 and SMN2 genes based on DHPLC

Forche et al. 2008 PLoS Biology
Forche et al. 2008 PLoS Biology

... been observed. Instead, tetraploid products of mating can be induced to undergo efficient, random chromosome loss, often producing strains that are diploid, or close to diploid, in ploidy. Using SNP and comparative genome hybridization arrays we have now analyzed the genotypes of products from the C ...
Abstracts - Parthenon Management Group
Abstracts - Parthenon Management Group

... The field of epigenetics explores the molecular mechanisms behind differential gene expression and the way in which related molecular marks are passed on during cell division. The most studied of these marks involve DNA methylation and histone modifications. The study of brain epigenetics can advanc ...
Product description P048-C1-0315 LMNA-MYOT - MRC
Product description P048-C1-0315 LMNA-MYOT - MRC

... Data normalisation should be performed within one experiment. Only samples purified by the same method should be compared. Confirmation of most exons deletions and amplifications can be done by e.g. Southern blotting, long range PCR, qPCR, FISH. Note that Coffalyser, the MLPA analysis tool developed ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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