molecular marker-based characterization of barley powdery mildew
... The control of pathogens is usually achieved by the application of fungicides during the growing season, but such practice has associated cost penalties as well as often unpredictable effects on wildlife biodiversity. Moreover, the use of pesticides is unacceptable in organic agriculture. The combin ...
... The control of pathogens is usually achieved by the application of fungicides during the growing season, but such practice has associated cost penalties as well as often unpredictable effects on wildlife biodiversity. Moreover, the use of pesticides is unacceptable in organic agriculture. The combin ...
Serotype IX, a Proposed New Streptococcus agalactiae
... precipitation tests (46). The Lancefield antigen extract was prepared as follows. A freeze-dried culture of GBS was suspended in serum broth, and a drop was plated onto 5% horse blood agar and incubated at 36°C overnight. The next day, a few individual colonies were inoculated into 5 ml glucose brot ...
... precipitation tests (46). The Lancefield antigen extract was prepared as follows. A freeze-dried culture of GBS was suspended in serum broth, and a drop was plated onto 5% horse blood agar and incubated at 36°C overnight. The next day, a few individual colonies were inoculated into 5 ml glucose brot ...
In Memoriam: Jacques Monod (1910–1976)
... time by the b-galactosides. A new category of enzymes, a ‘‘pump’’ responsible for accumulation of small molecules ...
... time by the b-galactosides. A new category of enzymes, a ‘‘pump’’ responsible for accumulation of small molecules ...
Analysis of the Molecular Basis of Flowering Time Variation in
... alleles are fully functional and confer very late flowering in the presence of the strong Col FLC allele. Given the identical amino acid sequence, it is likely that the three accessions Shakhdara, Kondara, and Kz-9 carry the same functional FRI allele. The F1 plants from the cross of Kz-9 to FRI(Sf2 ...
... alleles are fully functional and confer very late flowering in the presence of the strong Col FLC allele. Given the identical amino acid sequence, it is likely that the three accessions Shakhdara, Kondara, and Kz-9 carry the same functional FRI allele. The F1 plants from the cross of Kz-9 to FRI(Sf2 ...
Molecular cloning of a laccase isozyme gene from
... his4) were purchased from Invitrogen. Cloning procedures were performed using a laboratory stock of E. coli DH5α and One Shot INVαFh Chemically Competent E. coli (Invitrogen). PCR products were cloned into the pCR2.1-Topo vector (Invitrogen). The vectors pPIC3.5 and pPIC9 (Invitrogen) were used for ...
... his4) were purchased from Invitrogen. Cloning procedures were performed using a laboratory stock of E. coli DH5α and One Shot INVαFh Chemically Competent E. coli (Invitrogen). PCR products were cloned into the pCR2.1-Topo vector (Invitrogen). The vectors pPIC3.5 and pPIC9 (Invitrogen) were used for ...
Parallel Evolution of Copy-Number Variation across Continents in
... varying selection. In Drosophila melanogaster, the latitudinal clines across the eastern coasts of Australia and North America appear to be examples of this type of selection, with recent studies showing that a substantial portion of the D. melanogaster genome exhibits allele frequency differentiati ...
... varying selection. In Drosophila melanogaster, the latitudinal clines across the eastern coasts of Australia and North America appear to be examples of this type of selection, with recent studies showing that a substantial portion of the D. melanogaster genome exhibits allele frequency differentiati ...
Integrated mapping and characterization of the gene underlying the
... as normal or palmate with from three to five rather shallow sinuses, but other leaf shapes such as subokra, okra and superokra with variable depth of indentations also exist. The leaf shape of G. barbadense is defined as Sea Island-type, and is similar to subokra observed in G. hirsutum, having mode ...
... as normal or palmate with from three to five rather shallow sinuses, but other leaf shapes such as subokra, okra and superokra with variable depth of indentations also exist. The leaf shape of G. barbadense is defined as Sea Island-type, and is similar to subokra observed in G. hirsutum, having mode ...
SALSA MLPA probemix P222-A2 LCA mix-2 - MRC
... aforementioned genes in a DNA sample. Heterozygous deletions of recognition sequences should give a 3550% reduced relative peak area of the amplification product of that probe. Note that a mutation or polymorphism in the sequence detected by a probe can also cause a reduction in relative peak area, ...
... aforementioned genes in a DNA sample. Heterozygous deletions of recognition sequences should give a 3550% reduced relative peak area of the amplification product of that probe. Note that a mutation or polymorphism in the sequence detected by a probe can also cause a reduction in relative peak area, ...
Differential Enzyme Targeting As an Evolutionary Adaptation to
... alignment (fig. 3B) of the 55–amino acid sequence encoded by exon 1 downstream of, and including, the second translation start site revealed a high degree of conservation with an 84% sequence identity across all seven species. In contrast, a similar analysis of the 22–amino acid sequence (19 amino a ...
... alignment (fig. 3B) of the 55–amino acid sequence encoded by exon 1 downstream of, and including, the second translation start site revealed a high degree of conservation with an 84% sequence identity across all seven species. In contrast, a similar analysis of the 22–amino acid sequence (19 amino a ...
Evaluation of computational metabolic
... by the genome center that annotated the genome. We call this matching process ‘linking enzymes to reactions’. The motivations behind this process are as follows. (1) Reactions are the bridges between enzymes and pathways, in the sense that our pathway ontology (Karp and Paley, 1994) directly links a ...
... by the genome center that annotated the genome. We call this matching process ‘linking enzymes to reactions’. The motivations behind this process are as follows. (1) Reactions are the bridges between enzymes and pathways, in the sense that our pathway ontology (Karp and Paley, 1994) directly links a ...
Identity elements in tRNA-mediated transcription
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
Male-killing i n two species of insect Wolbachia 1~ THE ROYAL
... Interestingly, these two sequences differed from each other in 38 nucleotides (6.92% divergence, uncorrected for multiple hits). The three sequences (one from A. encedon, two from A. bipunctata: EMBL accession numbers AJ130714~AJ130716) were aligned to published sequences of B-group Wolbachia and tw ...
... Interestingly, these two sequences differed from each other in 38 nucleotides (6.92% divergence, uncorrected for multiple hits). The three sequences (one from A. encedon, two from A. bipunctata: EMBL accession numbers AJ130714~AJ130716) were aligned to published sequences of B-group Wolbachia and tw ...
Fig. - DIGITAL.CSIC, el repositorio institucional
... than the filaments elicited by GTP16. Two additional proteins, FtsA and ZipA, are required to attach the FtsZ polymers to the cytoplasmic membrane, forming the first complex of the divisome – the proto-ring. The position of the Z-ring is regulated by two negative control systems that independently i ...
... than the filaments elicited by GTP16. Two additional proteins, FtsA and ZipA, are required to attach the FtsZ polymers to the cytoplasmic membrane, forming the first complex of the divisome – the proto-ring. The position of the Z-ring is regulated by two negative control systems that independently i ...
PPT
... The number of interactions for each fold = the number of other folds it is found to contact in the PDB ...
... The number of interactions for each fold = the number of other folds it is found to contact in the PDB ...
informe tecnológico de patentes
... disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant al ...
... disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant al ...
Computational Definition of
... sequence families comprising 2069 putative exonic enhancers and 974 putative exonic silencers. Representatives of each class indeed functioned as enhancers or silencers when inserted into a test exon and assayed in transfected mammalian cells. As a class, the enhancer sequencers were more prevalent ...
... sequence families comprising 2069 putative exonic enhancers and 974 putative exonic silencers. Representatives of each class indeed functioned as enhancers or silencers when inserted into a test exon and assayed in transfected mammalian cells. As a class, the enhancer sequencers were more prevalent ...
Methods Five 1536-SNP GoldenGate assays (Fig. 1) Three pilot
... Relationships between SCRI or IPK contigs and HarvEST:Barley assembly #32 unigenes were determined by finding within assembly #32 unigenes ESTs that were the origination points for SCRI and IPK amplicon sequencing, or in a few cases using BLAST to find a strong match to the amplicon consensus sequen ...
... Relationships between SCRI or IPK contigs and HarvEST:Barley assembly #32 unigenes were determined by finding within assembly #32 unigenes ESTs that were the origination points for SCRI and IPK amplicon sequencing, or in a few cases using BLAST to find a strong match to the amplicon consensus sequen ...
Mapping Mendelian Factors Underlying Quantitative ... Using RFLP Linkage Maps Eric
... differences may also merit attention. Despite the lack of a mathematical guarantee, QTLs with large effects may nonetheless besegregating.When there is no prior evidence of both high and low alleles in the same strain, one maywish to proceed as in the previous paragraph. When there is evidence (as w ...
... differences may also merit attention. Despite the lack of a mathematical guarantee, QTLs with large effects may nonetheless besegregating.When there is no prior evidence of both high and low alleles in the same strain, one maywish to proceed as in the previous paragraph. When there is evidence (as w ...
What is p53
... initiates apoptosis if the damage to the cell is severe (this protects the organism from the growth of damaged cells, and so loss of p53 function is a key step in the neoplastic cascade). Mediators of this effect: bax [Ref.] often as a tumour suppressor: Mutations in p53 can cause cells to become on ...
... initiates apoptosis if the damage to the cell is severe (this protects the organism from the growth of damaged cells, and so loss of p53 function is a key step in the neoplastic cascade). Mediators of this effect: bax [Ref.] often as a tumour suppressor: Mutations in p53 can cause cells to become on ...
Xq28 duplications
... outwards starting from where the short and long arms meet, at a point called the centromere (the area coloured yellow in the diagram above). Region q28 is at the bottom end of the long arm of chromosome X (marked with a red bar in the diagram). With any duplication, the amount of duplicated DNA can ...
... outwards starting from where the short and long arms meet, at a point called the centromere (the area coloured yellow in the diagram above). Region q28 is at the bottom end of the long arm of chromosome X (marked with a red bar in the diagram). With any duplication, the amount of duplicated DNA can ...
Single-stranded heteroduplex intermediates in l Red homologous
... Background: The Red proteins of lambda phage mediate probably the simplest and most efficient homologous recombination reactions yet described. However the mechanism of dsDNA recombination remains undefined. Results: Here we show that the Red proteins can act via full length single stranded intermed ...
... Background: The Red proteins of lambda phage mediate probably the simplest and most efficient homologous recombination reactions yet described. However the mechanism of dsDNA recombination remains undefined. Results: Here we show that the Red proteins can act via full length single stranded intermed ...