Determining the Transcription Factor Genes Populating a Fruit Fly
... The methodological goal of the RNAi screen was to reduce the expression of Drosophila melanogaster transcription factor genes individually along the dorsal midline of the abdomen. The reduction of gene expression occurs through the exploitation of the endogenous fruit fly RNA interference pathway. I ...
... The methodological goal of the RNAi screen was to reduce the expression of Drosophila melanogaster transcription factor genes individually along the dorsal midline of the abdomen. The reduction of gene expression occurs through the exploitation of the endogenous fruit fly RNA interference pathway. I ...
Alternatively Spliced Genes
... Fig. 3 Sequences of human spliceosomal UsnRNAs and their interactions with splice sites. Panel (a) Sequences and secondary structures of spliceosomal snRNAs: U1, U2, U4/6 (for the U2 type), and U11, U12, U4atac/U6atac snRNA (for the U12 type) as well as U5snRNAs (for both classes). The 5 to 3 orie ...
... Fig. 3 Sequences of human spliceosomal UsnRNAs and their interactions with splice sites. Panel (a) Sequences and secondary structures of spliceosomal snRNAs: U1, U2, U4/6 (for the U2 type), and U11, U12, U4atac/U6atac snRNA (for the U12 type) as well as U5snRNAs (for both classes). The 5 to 3 orie ...
Regulation of limb bud initiation and limbtype
... LPM of the flank and are thought to specify the levels at which the limb bud will appear and, thus, their identity (Cohn et al., 1997; Cohn and Tickle, 1999) (Fig. 1A). For example, Hoxc4-5 is expressed mostly in the mesenchyme of the presumptive forelimb area (Savard et al., 1988; Tabin, 1989; Molve ...
... LPM of the flank and are thought to specify the levels at which the limb bud will appear and, thus, their identity (Cohn et al., 1997; Cohn and Tickle, 1999) (Fig. 1A). For example, Hoxc4-5 is expressed mostly in the mesenchyme of the presumptive forelimb area (Savard et al., 1988; Tabin, 1989; Molve ...
Transvection Is Common Throughout the Drosophila
... et al. 2004). These lineages are further subdivided into hemilineages based on Notch activity (Truman et al. 2010), and these hemilineages can also be recognized by their neuron position and morphology. To have a genetic handle on these populations of neurons, we have been taking advantage of a rece ...
... et al. 2004). These lineages are further subdivided into hemilineages based on Notch activity (Truman et al. 2010), and these hemilineages can also be recognized by their neuron position and morphology. To have a genetic handle on these populations of neurons, we have been taking advantage of a rece ...
Mouse Lefty2 and Zebrafish Antivin Are Feedback
... and display a phenotype identical to sqt;cyc double mutants (Gritsman et al., 1999). These results indicate that Nodal signals not only require TGFb-like receptors, but are also dependent on extracellular cofactors of the EGF-CFC family. A novel family of TGFb molecules implicated in vertebrate embr ...
... and display a phenotype identical to sqt;cyc double mutants (Gritsman et al., 1999). These results indicate that Nodal signals not only require TGFb-like receptors, but are also dependent on extracellular cofactors of the EGF-CFC family. A novel family of TGFb molecules implicated in vertebrate embr ...
Modified uridine at wobble position in tRNA of
... which are derivatives of the four normal nucleosides, adenosine (A), guanosine (G), cytidine (C), and uridine (U). Although modified nucleosides are present at many positions in tRNAs, two positions in the anticodon region, position 34 (wobble position) and position 37, show the largest variety of m ...
... which are derivatives of the four normal nucleosides, adenosine (A), guanosine (G), cytidine (C), and uridine (U). Although modified nucleosides are present at many positions in tRNAs, two positions in the anticodon region, position 34 (wobble position) and position 37, show the largest variety of m ...
The Homothorax homeoprotein activates the nuclear localization of
... have P-tagged mutants with the posterior eye pigmentation phenotype (Pradip Sinha, pers. comm.) Complementation tests showed that tss and hth are different loci (data not shown). Mutant hth and tss embryos have similar head defects. Two tss mutants (tss47 and tss470) were also defective for hth. The ...
... have P-tagged mutants with the posterior eye pigmentation phenotype (Pradip Sinha, pers. comm.) Complementation tests showed that tss and hth are different loci (data not shown). Mutant hth and tss embryos have similar head defects. Two tss mutants (tss47 and tss470) were also defective for hth. The ...
as Adobe PDF - Edinburgh Research Explorer
... the specification of subtypes of VM in mice (Lettice et al. 1999) and flies (Azpiazu and Frasch 1993). This high level of functional conservation suggests that the underlying transcriptional circuitry governing VM development may also be conserved. To date, most progress has been made in elucidating ...
... the specification of subtypes of VM in mice (Lettice et al. 1999) and flies (Azpiazu and Frasch 1993). This high level of functional conservation suggests that the underlying transcriptional circuitry governing VM development may also be conserved. To date, most progress has been made in elucidating ...
Par-1
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
Endonucleolytic processing of CCAless tRNA precursors by RNase
... residue. Lastly, tRNAs with long 5¢ extensions are poor substrates for cleavage, suggesting that for some tRNAs, processing of the 5¢ end by RNase P may have to precede RNase Z cleavage. Keywords: CCA/endonuclease/tRNA/RNase Z/3¢ tRNase ...
... residue. Lastly, tRNAs with long 5¢ extensions are poor substrates for cleavage, suggesting that for some tRNAs, processing of the 5¢ end by RNase P may have to precede RNase Z cleavage. Keywords: CCA/endonuclease/tRNA/RNase Z/3¢ tRNase ...
Identity elements in tRNA-mediated transcription
... whereas the specifier (AAC) mutant was induced at only a very low level by Asn depletion (Fig. 3, lane 2). However, under conditions of overexpression of the lactococcal tRNATrp in the specifier (AAC) mutant, it became evident that induction was still possible, albeit at a low level, by Trp depletio ...
... whereas the specifier (AAC) mutant was induced at only a very low level by Asn depletion (Fig. 3, lane 2). However, under conditions of overexpression of the lactococcal tRNATrp in the specifier (AAC) mutant, it became evident that induction was still possible, albeit at a low level, by Trp depletio ...
A MIAME-compliant Microarray Database
... • In the first experiment, they found some genes regulated by addition of GA-4 • They made GFP fusions to three of these genes ...
... • In the first experiment, they found some genes regulated by addition of GA-4 • They made GFP fusions to three of these genes ...
p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA
... of p68 protein levels (Barta and Iggo 1995), again suggesting that this intron is functionally important. However, in mammalian tissues, there is no obvious positive, or negative, correlation between levels of intron-containing p68 RNA and p68 protein (Stevenson et al. 1998). Therefore, the function ...
... of p68 protein levels (Barta and Iggo 1995), again suggesting that this intron is functionally important. However, in mammalian tissues, there is no obvious positive, or negative, correlation between levels of intron-containing p68 RNA and p68 protein (Stevenson et al. 1998). Therefore, the function ...
Evolutionary dynamics of nematode operons
... abundant in nematode genomes. The “one-way street” hypothesis asserts that once formed by chance, operons are very difficult to break, because the breakage would leave downstream genes in an operon without a promoter, and hence, unexpressed. To test this hypothesis, we analyzed the presence and abse ...
... abundant in nematode genomes. The “one-way street” hypothesis asserts that once formed by chance, operons are very difficult to break, because the breakage would leave downstream genes in an operon without a promoter, and hence, unexpressed. To test this hypothesis, we analyzed the presence and abse ...
Tracing the Thread of Plastid Diversity through the Tapestry of Life
... the gram-negative cyanobacterial cell. This view is bolstered by the recent determination that Toc75, a key component of the plant plastid’s protein import apparatus, is homologous to a cyanobacterial secretory protein, both localized to the outer plastid envelope (reported in two papers submitted w ...
... the gram-negative cyanobacterial cell. This view is bolstered by the recent determination that Toc75, a key component of the plant plastid’s protein import apparatus, is homologous to a cyanobacterial secretory protein, both localized to the outer plastid envelope (reported in two papers submitted w ...
X-Chromosome dosage compensation
... 3. Dosage compensation proteins assemble onto hermaphrodite X chromosomes to regulate gene expression and also function separately in chromosome segregation ..................................................................... 3 4. Sex-specific targeting of the dosage compensation complex to hermaph ...
... 3. Dosage compensation proteins assemble onto hermaphrodite X chromosomes to regulate gene expression and also function separately in chromosome segregation ..................................................................... 3 4. Sex-specific targeting of the dosage compensation complex to hermaph ...
Bridging the transgenerational gap with epigenetic memory
... in mammals during the 1980s provided the first indication that epigenetic modifications, such as DNA methylation, were not entirely erased between generations and could underlie transgenerational epigenetic inheritance [6–11]. It was also around this time that several cases of transgenerational epig ...
... in mammals during the 1980s provided the first indication that epigenetic modifications, such as DNA methylation, were not entirely erased between generations and could underlie transgenerational epigenetic inheritance [6–11]. It was also around this time that several cases of transgenerational epig ...
of Lactobacillus pentosus
... Davis et al., 1984, Davis and Henderson, 1987). This xylE gene was not found within the cluster of xyl genes but is present on a separate locus on theE. coli chromosome. The xylR gene codes for a regulatory protein involved in the activation of expression of the xylose genes in the presence of D-xyl ...
... Davis et al., 1984, Davis and Henderson, 1987). This xylE gene was not found within the cluster of xyl genes but is present on a separate locus on theE. coli chromosome. The xylR gene codes for a regulatory protein involved in the activation of expression of the xylose genes in the presence of D-xyl ...
Nonsensemediated decay of glutathione peroxidase 1 mRNA in the
... Maquat, 1998). The ®nding for T-cell receptor-b transcripts that NMD is elicited by a nonsense codon located as close as eight nucleotides upstream of an unnatural 3¢most exon±exon junction (Carter et al., 1996) may provide an exception to the rule or re¯ect the presence of an intronindependent dest ...
... Maquat, 1998). The ®nding for T-cell receptor-b transcripts that NMD is elicited by a nonsense codon located as close as eight nucleotides upstream of an unnatural 3¢most exon±exon junction (Carter et al., 1996) may provide an exception to the rule or re¯ect the presence of an intronindependent dest ...
Computational Identification of Plant MicroRNAs and
... MicroRNAs (miRNAs) are ⵑ21-nucleotide RNAs, some of which have been shown to play important generegulatory roles during plant development. We developed comparative genomic approaches to systematically identify both miRNAs and their targets that are conserved in Arabidopsis thaliana and rice (Oryza s ...
... MicroRNAs (miRNAs) are ⵑ21-nucleotide RNAs, some of which have been shown to play important generegulatory roles during plant development. We developed comparative genomic approaches to systematically identify both miRNAs and their targets that are conserved in Arabidopsis thaliana and rice (Oryza s ...
Puffs and PCR: the in vivo dynamics of early gene
... developed in the tissue coordination model (Burtis et al., 1990; Thummel et al., 1990) which suggests that one aspect of the stage and tissue-specific response to hormone may consist of differences in the levels of the different isoforms of the transcripts of the early puffs induced by ecdysone in e ...
... developed in the tissue coordination model (Burtis et al., 1990; Thummel et al., 1990) which suggests that one aspect of the stage and tissue-specific response to hormone may consist of differences in the levels of the different isoforms of the transcripts of the early puffs induced by ecdysone in e ...
informe tecnológico de patentes
... disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant al ...
... disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant al ...
a complex voyage to the X chromosome
... entry site (CES) recognition (Scott et al., 2000; Morales et al., 2004; Li et al., 2005). The coiled coil (CC) domain and PEHE domain (named for its characteristic amino acid composition) are shown (Marin, 2003). (B) The RING finger motif of the MSL2 protein is involved in interactions with MSL1, wh ...
... entry site (CES) recognition (Scott et al., 2000; Morales et al., 2004; Li et al., 2005). The coiled coil (CC) domain and PEHE domain (named for its characteristic amino acid composition) are shown (Marin, 2003). (B) The RING finger motif of the MSL2 protein is involved in interactions with MSL1, wh ...
Inhibition of RNA Synthesis by Anthracycline Analogs
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...