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Download Dendritic organization of sensory input to cortical neurons in vivo
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Transcript
Journal Club Yu Kai 2010/09/27 1 Introduction In sensory cortical areas, neurons are turned to specific stimulus features. In the present paper, the authers investigate the characteristics of the synaptic input that cortical neurons receive to generate their output firing pattern in the visual cortex of mouse. 2 The mechanism by which neurons generate signals: Input in dendritic synapase Integration in neuron soma Output as spikes 3 Isolation of input from neuron in vivo They use the approach combining high-speed two-photon imaging with whole-cell patch recordings to identify local sub-threshold calcium signals that correspond to orientationspecific synaptic inputs 4 Isolation of input from neuron in vivo Whole-cell current-clamp recordings of responses to drifting gratings of different orientations. Three trials were superimposed. 5 Isolation of input from neuron in vivo: Calcium transient in distal dendrites 6 Isolation of input from neuron in vivo: Calcium recordings from five neighbouring dendritic regions 7 Isolation of input from neuron in vivo: Exclusive the dendritic NMDA spike 8 Results: Visually evoked action potentials, subthreshold depolarizations 9 Results:Distribution of dendritic hotspots (red dots) to the prefered orientation stimuli 10 Results: Orientation perfermance different in the same dentrite 11 Conclusion: The results reveal basic insights into the dendritic organization of sensory inputs to neurons of the visual cortex in vivo. • Identified discrete dendritic hotspots as synaptic entry sites for specific sensory features • Afferent sensory inputs with the same orientation preference are widely dispersed over thedendritic tree and do not converge on single dendrites • Neurons with a highly tuned output signal receive input signals that are heterogeneous The results support a neuronal integration model involving summation of distributed inputs, rather than models that stress the role of convergent inputs to single dendrites. 12 Progress Report 13 Verify responsibilities of MGB locus which very close to the injection position 14 Injetion Lidocaine into the MGB while continuous recording in A1 Sweep # F:\yukai data\20100915\20100915_006G_RX5-1_002.src Lidocaine Injection 0 600 1200 1800 2400 3000 msec Raster Plot indicate the lidocaine injection cause inversible change on the firing pattern of responses to acoustic stimulation. 15 Corresponding recording before lidocaine injection 16 After 17 Intracellular Recording of cortical neuron in layer2/3 Before lidocaine into MGB 18 After 19
