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Anti-C. tetani Tetanus Toxin Monoclonal antibody, Clone UfuI7
Cat.No: DCABY-466
Lot. No. (See product label)
PRODUCT INFORMATION
Specificity
Mouse anti Clostridium tetani Tetanus Toxin antibody, clone UfuI7 recognizes the tetanus toxin, a
neurotoxin produced by the vegetative spore of Clostridium tetani.
Target
C. tetani Tetanus Toxin
Immunogen
Supernatant from Clostridium tetani.
Isotype
IgG1
Source/Host
Mouse
Species Reactivity
C. tetani
Clone
UfuI7
Conjugate
Unconjugated
Applications
ELISA, Functional Assays, Western Blot
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Format
Purified IgG - liquid
Size
200 μg
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide (NaN3)
Storage
Store at +4°C or at -20°C if preferred.Storage in frost-free freezers is not recommended.This product
should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Background
Creative Diagnostics. All rights reserved
45-1 Ramsey Road Shirley, NY 11967, USA
Tel: 1-631-624-4882 Fax: 1-631-938-8221
E-mail: [email protected]
www.creative-diagnostics.com
1
Introduction
Clostridium tetani?is a box-car shaped,?anaerobic?bacterium?of the genus species?Clostridium. Like
other?Clostridium?genus species, it is?Gram-positive, and its appearance on a?gram
stain?resemblestennis rackets?or drumsticks. C. tetani?is found as?spores?in soil or in
the?gastrointestinal tractof animals.?C. tetani?produces a potent biological toxin,?tetanospasmin, and
is the causative agent oftetanus, a disease characterized by painful muscular spasms that can lead to
respiratory failure and, in up to 40% of cases, death.Tetanospasmin released in the wound is
absorbed into the circulation and reaches the ends of motor neurons all over the body. The toxin acts
at several sites within the central nervous system, including nerve terminals, the spinal cord, and brain,
and within the sympathetic nervous system. By binding to peripheral motor neuron terminals, the toxin
enters the nerve axons, and is transported across synaptic junctions to the nerve-cell body in the brain
stem and spinal cord by retrograde intraneuronal transport, until it reaches the central nervous system,
where it rapidly binds to gangliosides at the presynaptic membrane of inhibitory motor nerve endings.
The clinical manifestations of tetanus are caused when tetanus toxin blocks inhibitory impulses, by
interfering with the release of neurotransmitters, including glycine and gamma-aminobutyric acid.
These inhibitory neurotransmitters inhibit the alpha motor neurons. With diminished inhibition, the
resting firing rate of the alpha motor neuron increases, producing rigidity, unopposed muscle
contraction and spasm. Characteristic features are risus sardonicus (a rigid smile), trismus (commonly
known as 'lock-jaw'), and opisthotonus (rigid, arched back). Seizures may occur, and the autonomic
nervous system may also be affected. Tetanospasmin appears to prevent the release of
neurotransmitters by selectively cleaving a component of synaptic vesicles called synaptobrevin II.
Loss of inhibition also affects preganglionic sympathetic neurons in the lateral gray matter of the spinal
cord and produces sympathetic hyperactivity and high circulating catecholamine levels. Hypertension
and tachycardia alternating with hypotension and bradycardia may develop.
Keywords
Clostridium tetani Tetanus Toxin
Antigen Gene Information
Pathway
Cell adhesion molecules (CAMs); Intestinal immune network for IgA production; Toll-like receptor
signaling pathway
Creative Diagnostics. All rights reserved
45-1 Ramsey Road Shirley, NY 11967, USA
Tel: 1-631-624-4882 Fax: 1-631-938-8221
E-mail: [email protected]
www.creative-diagnostics.com
2