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Chromosome and genome 11-16-2015 Genome: whole set of DNA to store all genetic information Circular versus linear chromsome Human Genome • How many chromosomes does each human cell have? – 22 pairs of autosomal chromosome and 1 pair of sex chromosomes • What is the size of the human genome? – The human genome is made up of 3 billion base pairs • How many genes does the Human Genome code for? – Define the meaning of gene first! Chromosomes p Centromere q Chromosome 5 Chromosomes as seen at metaphase during cell division Telomere DNA and protein cap Ensures replication to tip Tether to nuclear membrane Light bands Replicate early in S phase Less condensed chromatin Transcriptionally active Gene and GC rich Short arm p (petit) Centromere Joins sister chromatids Long arm q Essential for chromosome segregation at cell division 100s of kilobases of repetitive DNA: some nonspecific, some chromosome specific Dark (G) bands Telomere Replicate late Contain condensed chromatin AT rich Total Genes On Chromosome 17: 723 373 genes in region marked red, 20 are shown FZD2 AKAP10 ITGB4 KRTHA8 WD1 SOST Genes are arranged in linear order on chromosomes MPP3 MLLT6 STAT3 BRCA1 GFAP NRXN4 NSF NGFR Chromosome 17 source: Human Genome Project CACNB1 HOXB9 HTLVR ABCA5 CDC6 ITGB3 breast cancer 1, early onset Genome Comparison Organism # of Genes Genome Size (bp) E.coli 4,200 4,600,200 S. cervisiae (yeast) 6,000 12,000,000 C. elgans (nematode) 14,000 100,000,000 D. melanogaster (fruit fly) 14,000 140,000,000 A. thaliana (mustard plant) 24,000 120,000,000 Human 19,000 3,000,000,000 Human Genome • What is a gene? – A gene is segment of a genome that encodes a single specific end product (a protein or RNA) or a functional unit or information • How much of the genome are “genes”? – ~1.5% of the genome for protein coding • What accounts for the rest of the DNA in the genome? Human Genome • Other items in genome – Introns – Repeated sequences (telomeres, centromeres) – Non-coding RNAs • • • • tRNA, rRNA, snRNA. miRNA Long non-coding RNA Functional or noise???? – Transposons (SINES and LINES) – Psuedogenes (genes that no longer produce functional products) Transposons: Mobile DNA • Class II transposons: DNA (transposons) that moves directly from place to place. • Class I transposons: retrotransposons – That first transcribe the DNA into RNA and then – use reverse transcriptase to make a DNA copy of the RNA to insert in a new location Discovery of transposons Barbara McClintock (1902-1992) Nobel prize in Physiology and Medicine 1983 Mobile genetic elements in maize 1940-1950 Discovery of TE - study of chromosomal breakage - increased frequency in certain site (= marker „dissociation“ Ds) - location of Ds (dissociator) was unstable after crossing with some lines with Ac (activator) (= line carrying „activator“ Ac) Discovery of TE - in one location – Ds insertion was connected with loss of purple pigment of endosperm - after crossing with activator line pigment synthesis was recovered in some cells Three of the many types of mobile genetic elements found in bacteria Transposase gene: encoding enzymes for DNA breakage and joining Red segments: DNA sequences as recognition sites for enzymes Yellow segments: antibiotic genes Transposition: Cut and Paste (command/control-X and command/control-V in your computer) DNA-only What is wrong about class I element? How to handle such problem? The transposable element content of the human genome What we should know about Alu element! • All Alus are approximately 300 bp in length. • A single recognition site for the restriction enzyme AluI located near the middle of the Alu element. • Alu elements are found only in primates. • Human chromosomes contain about 1,000,000 Alu copies (10% of the total genome). • Alu is a "jumping gene" – a transposable DNA sequence that "reproduces" by copying itself and inserting into new chromosome locations. What we should know about Alu element! • No evidence that it is ever excised or lost from a chromosome locus. Each Alu insertion is the "fossil" of a unique transposition event that occurred only once in primate evolution. • Each Alu element has an internal promoter for RNA polymerase III. • However, Alu lacks the enzyme functions to produce a DNA copy of itself and to integrate into a new chromosome position Alu transposition via an RNA intermediate: L1 provides RT for reverse transcription and a nick on the host chromosome! Alu is a parasite of L1, which, in turn, is a relic of a retrovirus ancestor. What we should know about Alu element! • The rate of Alu transposition is about one in every 200 newborns today. • The vast majority of Alu insertions occur in noncoding regions and are thought to be neutral. • An Alu insertion in the NF-1 gene is responsible for neurofibromatosis I. • Alu insertions in introns of genes for tissue plasminogen activator (TPA) and angiotensin converter enzyme (ACE) are associated with heart disease. PV92, a human-specific Alu insertion on chromosome 16 How Alu jump! http://labcenter.dnalc.org/labs/dnafinge rprintalu/dnafingerprintalu_d.html DNA Nucleosome 細胞所有的遺傳資訊都完全登 錄在DNA上的ATGC序列嗎? Epigenetics: the study of mitotically and/or meiotically heritable changes in gene function that cannot be explained by changes in DNA sequence (Riggs et al. 1996) Epigenetic chromatin regulation A. Modification at the DNA level 1. cytosine methylation B. Histone modification - the histone code 1. Histone acetylation 2. Histone methylation 3. Histone phosphorylation 4. Histone ubiquitilation 5. Different types of histones The five nucleotides that make up the DNA Maintenance of methylation Brand eis, M., Ariel, M. & Cedar, H. ( 1 99 3 ) Bioessays 1 5 , 70 9-71 3. Genomic imprinting Some genes are expressed only from the maternal genome and some only from the paternal genome It is estimated that about 40 genes are imprinted and they can be found on several different chromosomes Imprinting is maintained by DNA methylation Why gene imprinting? Parent Offspring Conflict Hypothesis (Haig hypothesis) Conflict between male and female over allocation of maternal resources to offspring Paternally expressed genes would promote growth, maternally expressed genes should slow it down. For example: IGF2 (growth factor) / IGF2 receptor (make IGF2 inactive) IGF2 gene is paternal or maternal imprinted? How about IFG2 receptor? Epigenetic chromatin regulation A. Modification at the DNA level 1. cytosine methylation B. Histone modification - the histone code 1. Histone acetylation 2. Histone methylation 3. Histone phosphorylation 4. Histone ubiquitilation 5. Different types of histones Features of Histone Modifications • Covalently attached groups (usually to histone tails) Methyl Acetyl Phospho • Reversible and Dynamic – Enzymes that add/remove modification – Signals • Have diverse biological functions Ubiquitin SUMO Cell, 111:285-91, Nov. 1, 2002 Features of Histone Modifications • • Small vs. Large groups One or up to three groups per residue Ub = ~8.5 kDa H4 = 14 kDa Jason L J M et al. J. Biol. Chem. 2005 Types of Histone Modifications Bhaumik, Smith, and Shilatifard, 2007. Histone Modifications and Modifers • Writers: enzymes that add a mark • Readers: proteins that bind to and “interpret” the mark • Erasers: enzymes that remove a mark Tarakhovsky, A., Nature Immunology, 2010. Dynamic of chromosome structure • Copy Number Variation (CNV) in human genome • Total 1,447 CNV were identified across the 270 samples • Established average length of CNV regions per genome was over 20 million base pairs Nature 444:445 (2006) Copy number variation of amylase gene among different people with different diet! EXPANDING THE GENE CONCEPT BEYOND THE PROTEIN ENCODING SEQUENCESES of DNA: TRANSCRIPTION OF SOME GENES PRODUCES NONCODING RNAs Non-Coding RNA: Formerly known as “JUNK” A Key to Eukaryotic Complexity? Types of RNA CODING In translation (mRNA) NON-CODING In translation (tRNAs and rRNAs) In RNA processing: ribozymes Regulatory RNAs: Riboswitch; microRNA and lincRNA Capping by Branching: A New Ribozyme Makes Tiny Lariats Science 309: 1350-1; 2005 A decade of riboswitch Cell 152: 17-24; 2013 What is miRNA? 51 Characteristics of miRNAs • • • • • • Small non-coding double stranded RNAs Approximately 19-22 nt long Repress activity of complementary mRNAs Regulate 30% of mammalian gene products 1 miRNA = hundreds of mRNAs Many are conserved between vertebrates and invertebrates Genomic Organization miRNA processing Microprocessor Complex Differences in miRNA Mode of Action CORRELATION OF MIR EXPRESSION WITH PROGRESSION AND PROGNOSIS OF GASTRIC CANCER* PATIENTS: 181 patients from 2 cohorts (Japan) CLASSIFICATION: Stages I-IV Diffuse vs. Intestinal type ANALYSIS: • Custom miR microarray chip (Ohio State Univ.) • miR expression in 160 paired samples (tumor vs. non-tumor) • Correlations of miR expression vs. stage, type and prognosis (survival) * Lancet Oncol. 11,136, 2010 MiRs AS PROGNOSTIC FACTORS: GASTRIC CANCER SURVIVAL* Intestinal-Type Gastric Cancer miR-495 HAZARD RATIO (disease free survival) 5 4 3.2 3 2 1 0 Stages I-II Stages III-IV HAZARD RATIO (disease free survival) 10 9 8 7 6 5 4 3 2 1 0 miR-199 Let-7g high low high low low high I-II III-IV I-II III-IV I-II III-IV ANTIMETASTATIC ACTIVITY OF AHR AGONISTS IN ER BREAST CANCER (Mol Cancer Therap. 11, 108-118, 2012). Ligand activated Ahr AhR arnt miR-335 Normal cells Preneoplastic miR-335cells Cancer cells (Invasive carcinoma) Metastasis SOX4 SOX4 and other miR-335 regulated metastatic mRNAs SOX4 SOX4 and other miR-335 regulated proteins What is lincRNAs? Large intergenic noncoding RNAs (lincRNAs) are emerging as key regulators of diverse cellular processes. Determining the function of individual lincRNAs remains a challenge. Recent advances in RNA sequencing (RNA-seq) and computational methods allow for an unprecedented analysis of such transcripts. Cell July 3, 2013. Page 26 Cell nucleus is a highly organized structure just like a Rome city! Long Noncoding RNAs May Alter Chromosome’s 3D Structure 24 MAY 2013 VOL 340 SCIENCE page 910 RNA world • Carry information (DNA) • Catalyze chemical reaction (protein enzyme) • Nutrient sensor to control gene expression (protein receptor) • Broadly control gene expression through mRNA stability, translational efficiency etc. (protein activator or repressor) • Global control nuclear and chromosome structure.(Histone code)