Cleavage Close to the End of DNA Fragments (linearized
... double-stranded base pairs between the end of the recognition site and the terminus of the fragment; this number does not include the single-stranded overhang from the initial cut. Since it has not been demonstrated whether these singlestranded nucleotides contribute to cleavage efficiency, 4 bases ...
... double-stranded base pairs between the end of the recognition site and the terminus of the fragment; this number does not include the single-stranded overhang from the initial cut. Since it has not been demonstrated whether these singlestranded nucleotides contribute to cleavage efficiency, 4 bases ...
Synthesis and Biophysical Studies of Nucleic Acid-Binding
... a triplex. These two hybridization mechanisms are only possible through relatively weak hydrogen bond interactions, resulting in highly variable hybridisation efficiencies depending on the number of the nucleotides in each strand sequence. Currently, DNA and RNA oligonucleotides have found only limi ...
... a triplex. These two hybridization mechanisms are only possible through relatively weak hydrogen bond interactions, resulting in highly variable hybridisation efficiencies depending on the number of the nucleotides in each strand sequence. Currently, DNA and RNA oligonucleotides have found only limi ...
Friedrich Miescher and the discovery of DNA
... In a first step, Miescher separated the leucocytes from the bandaging material and the serum (Miescher, 1869a, 1871d). This separation posed a problem for Miescher. Solutions of NaCl or a variety of alkaline or alkaline earth salt solutions used to wash the pus resulted in a bslimy swellingQ of the ...
... In a first step, Miescher separated the leucocytes from the bandaging material and the serum (Miescher, 1869a, 1871d). This separation posed a problem for Miescher. Solutions of NaCl or a variety of alkaline or alkaline earth salt solutions used to wash the pus resulted in a bslimy swellingQ of the ...
TaqMan® SNP Genotyping Assays User Guide
... APPENDIX C About the Drug Metabolism Enzyme genes . . . . . . . . . . . 68 Introduction to the Drug Metabolism Enzyme genes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 Drug Metabolism Enzyme classifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...
... APPENDIX C About the Drug Metabolism Enzyme genes . . . . . . . . . . . 68 Introduction to the Drug Metabolism Enzyme genes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 Drug Metabolism Enzyme classifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...
2.1.databases_intro - T
... •Write down the PubMed Identifier (the number in the PMID field) of that interesting paper you just find. It could be very useful in your subsequent search for related items such as associated gene and protein sequences ...
... •Write down the PubMed Identifier (the number in the PMID field) of that interesting paper you just find. It could be very useful in your subsequent search for related items such as associated gene and protein sequences ...
Wolbachia: master manipulators of invertebrate biology
... these8. All Wolbachia supergroups are monophyletic compared with other Rickettsiales (FIG. 1). Two supergroups (C and D) are commonly found in filarial nematodes, whereas the other six supergroups are found primarily in arthropods, in which A and B are the most common. Nematode-associated Wolbachia ...
... these8. All Wolbachia supergroups are monophyletic compared with other Rickettsiales (FIG. 1). Two supergroups (C and D) are commonly found in filarial nematodes, whereas the other six supergroups are found primarily in arthropods, in which A and B are the most common. Nematode-associated Wolbachia ...
VAAST 2 User`s Guide
... Fasta files are used within VAAST to provide a copy of the reference genome sequence. The requirements of the Fasta format for VAAST are pretty straightforward. Each sequence (a chromosome or a ...
... Fasta files are used within VAAST to provide a copy of the reference genome sequence. The requirements of the Fasta format for VAAST are pretty straightforward. Each sequence (a chromosome or a ...
Neurospora Spore Killers Sk-2 and Sk
... alleles do not affect crossover frequencies and can therefore be mapped precisely. Both r(Sk-2) and pr(Sk-2) are located within the recombination block region, while mod(pr) is located just outside of it (Figure 1). We are interested in another seemingly unrelated ascus-dominant phenomenon called me ...
... alleles do not affect crossover frequencies and can therefore be mapped precisely. Both r(Sk-2) and pr(Sk-2) are located within the recombination block region, while mod(pr) is located just outside of it (Figure 1). We are interested in another seemingly unrelated ascus-dominant phenomenon called me ...
Genetics Practice Test
... ____ 33. Exposing bacteria to radiation or certain chemicals can increase the frequency of mutations that occur within the population. _________________________ ____ 34. A polyploid plant has more than two copies of each gene. _________________________ ____ 35. Scientists use genetic markers to dete ...
... ____ 33. Exposing bacteria to radiation or certain chemicals can increase the frequency of mutations that occur within the population. _________________________ ____ 34. A polyploid plant has more than two copies of each gene. _________________________ ____ 35. Scientists use genetic markers to dete ...
The presence of an RHD pseudogene containing a
... hybrid genes encoding partial D antigens may predict a D-negative phenotype, and the presence of some RHD regions in genes encoding no D antigen may predict a D-positive phenotype. In order to avoid these complications, methods where more than 1 region of RHD is detected in a single PCR were introdu ...
... hybrid genes encoding partial D antigens may predict a D-negative phenotype, and the presence of some RHD regions in genes encoding no D antigen may predict a D-positive phenotype. In order to avoid these complications, methods where more than 1 region of RHD is detected in a single PCR were introdu ...
Rfam Documentation
... Our seed alignments are small, curated sets of representative sequences for each family, as opposed to an alignment of all known members. The seed alignment also has as a secondary structure annotation, which represents the conserved secondary structure for these sequences. The ideal basis for a new ...
... Our seed alignments are small, curated sets of representative sequences for each family, as opposed to an alignment of all known members. The seed alignment also has as a secondary structure annotation, which represents the conserved secondary structure for these sequences. The ideal basis for a new ...
Critical Factors for Successful Real-Time PCR
... In multiplex, real-time PCR, several genomic DNA targets are quantified simultaneously in the same reaction. Multiplex, real-time RT-PCR is a similar method, allowing simultaneous quantification of several RNA targets in the same reaction. The procedure can be performed either as two-step RT-PCR or ...
... In multiplex, real-time PCR, several genomic DNA targets are quantified simultaneously in the same reaction. Multiplex, real-time RT-PCR is a similar method, allowing simultaneous quantification of several RNA targets in the same reaction. The procedure can be performed either as two-step RT-PCR or ...
a method for detecting and typing of salmonella by multiplex pcr
... selling ready-made food is growing throughout the country. The veterinary service should have time to check the quality of all of these products. Only traditional bacteriological methods of isolation and identification of pathogens of toxicoinfection, which is not enough in terms of increasing turno ...
... selling ready-made food is growing throughout the country. The veterinary service should have time to check the quality of all of these products. Only traditional bacteriological methods of isolation and identification of pathogens of toxicoinfection, which is not enough in terms of increasing turno ...
Identification of ornamental fishes for biosecurity
... tion for retail purposes (McDowall, 2004), while terspecific hybrid organisms (Aliabadian et al., interspecific hybrids may have unpredictable in- 2009; Dasmahapatra & Mallet, 2006). The matermediate phenotypes (Mallet, 2005), making trilineal inheritance of mtDNA means any hybrid them additionally ...
... tion for retail purposes (McDowall, 2004), while terspecific hybrid organisms (Aliabadian et al., interspecific hybrids may have unpredictable in- 2009; Dasmahapatra & Mallet, 2006). The matermediate phenotypes (Mallet, 2005), making trilineal inheritance of mtDNA means any hybrid them additionally ...
Unequal Crossing-over in Unique PABP2 Mutations in Japanese
... repeats are responsible for a number of hereditary neuromuscular diseases.7,14,15 These pathologic repeat expansions can be explained by the slippage model. However, it has been proposed that tracts of approximately 25 to 35 perfect trinucleotide repeats are required for instability and expansion vi ...
... repeats are responsible for a number of hereditary neuromuscular diseases.7,14,15 These pathologic repeat expansions can be explained by the slippage model. However, it has been proposed that tracts of approximately 25 to 35 perfect trinucleotide repeats are required for instability and expansion vi ...
Unipro UGENE Manual
... You're going to analyze ChIP-Seq data with the Cistrome pipeline Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched from the UGENE graphical interface, an external tool needs a corresponding executable file. The list of the external tools can be found on thi ...
... You're going to analyze ChIP-Seq data with the Cistrome pipeline Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched from the UGENE graphical interface, an external tool needs a corresponding executable file. The list of the external tools can be found on thi ...
Unipro UGENE Manual
... You're going to analyze ChIP-Seq data with the Cistrome pipeline Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched from the UGENE graphical interface, an external tool needs a corresponding executable file. The list of the external tools can be found on thi ...
... You're going to analyze ChIP-Seq data with the Cistrome pipeline Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched from the UGENE graphical interface, an external tool needs a corresponding executable file. The list of the external tools can be found on thi ...
Attachment 2.2 Sequencing results
... and the presence in many tissues has been detected, the relative level of expression remains to be measured in the different tissues. The possibility of this transcript undergoing nonsense mediated decay also needs further research. For a more complete picture of the conservation of the AHR gene, mo ...
... and the presence in many tissues has been detected, the relative level of expression remains to be measured in the different tissues. The possibility of this transcript undergoing nonsense mediated decay also needs further research. For a more complete picture of the conservation of the AHR gene, mo ...
COLEOPTILE PHOTOTROPISM1 Gene Encoding
... maize (Zea mays), had been the major materials of phototropism research. The results indicated that the phototropism of coleoptiles is multiphasic in its dependence on light fluences and involves spatially and temporally separable response components (Iino, 2001). Detailed molecular genetic analysis ...
... maize (Zea mays), had been the major materials of phototropism research. The results indicated that the phototropism of coleoptiles is multiphasic in its dependence on light fluences and involves spatially and temporally separable response components (Iino, 2001). Detailed molecular genetic analysis ...
TaqMan® Drug Metabolism Genotyping Assays
... – Indicates a potentially hazardous situation that, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices. – Indicates a potentially hazardous situation that, if not avoided, could result in death or serious injury. – Indicates an imminently ha ...
... – Indicates a potentially hazardous situation that, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices. – Indicates a potentially hazardous situation that, if not avoided, could result in death or serious injury. – Indicates an imminently ha ...
wmgenes2 - Cucurbit Breeding
... they have dgdg genotype (Rhodes, 1986). The juvenile albino ja (Zhang et al., 1996b) gene causes reduced chlorophyll in seedling tissues, as well as leaf margins and fruit rind when plants are grown under short day conditions. The dominant gene Sp (Poole, 1944) causes round yellow spots to form on c ...
... they have dgdg genotype (Rhodes, 1986). The juvenile albino ja (Zhang et al., 1996b) gene causes reduced chlorophyll in seedling tissues, as well as leaf margins and fruit rind when plants are grown under short day conditions. The dominant gene Sp (Poole, 1944) causes round yellow spots to form on c ...
Section 1 The application - Office of the Gene Technology Regulator
... must follow when considering an application for a licence to intentionally release a genetically modified organism (GMO) into the environment. For a licence to be issued, the Regulator must be satisfied that the release will not pose any risks to human health and safety and the environment that can ...
... must follow when considering an application for a licence to intentionally release a genetically modified organism (GMO) into the environment. For a licence to be issued, the Regulator must be satisfied that the release will not pose any risks to human health and safety and the environment that can ...
PCR and qPCR product guide
... For more information visit: www.thermoscientific.com/pcr © 2011 Thermo Fisher Scientific Inc. All rights reserved. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in a ...
... For more information visit: www.thermoscientific.com/pcr © 2011 Thermo Fisher Scientific Inc. All rights reserved. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in a ...