molecular marker-based characterization of barley powdery mildew
... salinity (Stanca, 2003), as well as biotic stresses, such as fungal pathogens (Weibull et al., 2003). The control of pathogens is usually achieved by the application of fungicides during the growing season, but such practice has associated cost penalties as well as often unpredictable effects on wil ...
... salinity (Stanca, 2003), as well as biotic stresses, such as fungal pathogens (Weibull et al., 2003). The control of pathogens is usually achieved by the application of fungicides during the growing season, but such practice has associated cost penalties as well as often unpredictable effects on wil ...
Product description P018-G1 SHOX-v03 - MRC
... SHOX gene function. - Please note that single exon deletions have a considerable chance of being a false positive result, either due to non-pathogenic copy number variants (Benito-Sanz et al. 2011a), mutations within the probe binding site (Barca-Tierno et al. 2011) or due to impurities in the DNA. ...
... SHOX gene function. - Please note that single exon deletions have a considerable chance of being a false positive result, either due to non-pathogenic copy number variants (Benito-Sanz et al. 2011a), mutations within the probe binding site (Barca-Tierno et al. 2011) or due to impurities in the DNA. ...
Gel immobilization of acrylamide-modified single
... [18]. For parallel pyrosequencing, Biotage AB (Uppsala, Sweden) developed the PyroMark MD that features an integrated vacuum prep workstation for the fully automated preparation of ssDNA templates immobilized on streptavidin-coated beads. A recently reported third method to prepare ssDNA template in ...
... [18]. For parallel pyrosequencing, Biotage AB (Uppsala, Sweden) developed the PyroMark MD that features an integrated vacuum prep workstation for the fully automated preparation of ssDNA templates immobilized on streptavidin-coated beads. A recently reported third method to prepare ssDNA template in ...
Chapter 11 Slides
... • Bacteria have learned to "restrict" the possibility of attack from foreign DNA by means of "restriction enzymes" • Type II and III restriction enzymes cleave DNA chains at selected sites • Enzymes may recognize 4, 6 or more bases in selecting sites for cleavage • An enzyme that recognizes a 6-base ...
... • Bacteria have learned to "restrict" the possibility of attack from foreign DNA by means of "restriction enzymes" • Type II and III restriction enzymes cleave DNA chains at selected sites • Enzymes may recognize 4, 6 or more bases in selecting sites for cleavage • An enzyme that recognizes a 6-base ...
The American University in Cairo School of Science and Engineering
... using a wide variety of techniques such as insertional mutagenesis, site-directed and sitesaturation mutagenesis, random replacement mutagenesis, DNA shuffling, and random insertional deletional strand exchange mutagenesis (Babic et al., 2006). These experiments are mainly used in order to show how ...
... using a wide variety of techniques such as insertional mutagenesis, site-directed and sitesaturation mutagenesis, random replacement mutagenesis, DNA shuffling, and random insertional deletional strand exchange mutagenesis (Babic et al., 2006). These experiments are mainly used in order to show how ...
NO sensing by FNR: regulation of the Escherichia coli NO
... the ¯avohaemoglobin-encoding gene of E.coli, hmp, is upregulated by NO and RNS; this appears not to involve SoxRS (Poole et al., 1996). We have reported (MembrilloHernaÂndez et al., 1998) a mechanism of hmp gene regulation that involves interaction between S-nitrosothiols and Hcy. Intracellular Hcy ...
... the ¯avohaemoglobin-encoding gene of E.coli, hmp, is upregulated by NO and RNS; this appears not to involve SoxRS (Poole et al., 1996). We have reported (MembrilloHernaÂndez et al., 1998) a mechanism of hmp gene regulation that involves interaction between S-nitrosothiols and Hcy. Intracellular Hcy ...
Integrated mapping and characterization of the gene underlying the
... homeodomain leucine-zipper class I protein, whose closely related genes in several other plant species have been shown to be involved in regulating leaf morphology. The transcript levels of GhOKRA in shoot apices were positively correlated with the phenotypic expression of the okra leaf trait. Of th ...
... homeodomain leucine-zipper class I protein, whose closely related genes in several other plant species have been shown to be involved in regulating leaf morphology. The transcript levels of GhOKRA in shoot apices were positively correlated with the phenotypic expression of the okra leaf trait. Of th ...
PO 4
... The amino acids are joined together in the correct sequence to make part of a protein Ala ...
... The amino acids are joined together in the correct sequence to make part of a protein Ala ...
Strains of Salmonella typhimurium and Other
... subdivided, and the decision not to greatly subdivide the Escherichia group is no easier to justify than the decision to do so in Salmonella. CHARACTERISTICS OF STRAIN LT2 AND SOME OF ITS DERIVATIVES Strain LT2 has proven to be suitable for use as a representative of the wild type of S. typhimurium. ...
... subdivided, and the decision not to greatly subdivide the Escherichia group is no easier to justify than the decision to do so in Salmonella. CHARACTERISTICS OF STRAIN LT2 AND SOME OF ITS DERIVATIVES Strain LT2 has proven to be suitable for use as a representative of the wild type of S. typhimurium. ...
De novo DNA methylation through the 5′-segment
... Because allelic DNA methylation of ICRs is acquired during gametogenesis, ICRs are also called germline differentially methylated regions (gDMRs) (Ferguson-Smith, 2011; Kelsey and Feil, 2013; Tomizawa and Sasaki, 2012). Recent genome-wide profiling has, however, revealed that the number of oocyte- o ...
... Because allelic DNA methylation of ICRs is acquired during gametogenesis, ICRs are also called germline differentially methylated regions (gDMRs) (Ferguson-Smith, 2011; Kelsey and Feil, 2013; Tomizawa and Sasaki, 2012). Recent genome-wide profiling has, however, revealed that the number of oocyte- o ...
PDF
... Because allelic DNA methylation of ICRs is acquired during gametogenesis, ICRs are also called germline differentially methylated regions (gDMRs) (Ferguson-Smith, 2011; Kelsey and Feil, 2013; Tomizawa and Sasaki, 2012). Recent genome-wide profiling has, however, revealed that the number of oocyte- o ...
... Because allelic DNA methylation of ICRs is acquired during gametogenesis, ICRs are also called germline differentially methylated regions (gDMRs) (Ferguson-Smith, 2011; Kelsey and Feil, 2013; Tomizawa and Sasaki, 2012). Recent genome-wide profiling has, however, revealed that the number of oocyte- o ...
factor involved in dorsal-ventral axis formation and neurogenesis
... solid boxes numbered 1-6 represent the exons from cDNAs encoding the putative spi protein; the hatched region within exon 6 represents the coding sequence (see also Fig. 3). All of the cDNAs examined that encode spi are composed of two or more exons. The location of exon 1 in the genomic region has ...
... solid boxes numbered 1-6 represent the exons from cDNAs encoding the putative spi protein; the hatched region within exon 6 represents the coding sequence (see also Fig. 3). All of the cDNAs examined that encode spi are composed of two or more exons. The location of exon 1 in the genomic region has ...
Original article MOLECULAR CHARACTERISATION OF LUMPY
... characterisation of lumpy skin disease virus and sheeppox virus based on P32 gene. Bulg. J. Vet. Med. (online first). Lumpy skin disease virus (LSDV) and sheeppox virus (SPV) have a considerable economic impact on the cattle and small ruminant industry. They are listed in group A of contagious disea ...
... characterisation of lumpy skin disease virus and sheeppox virus based on P32 gene. Bulg. J. Vet. Med. (online first). Lumpy skin disease virus (LSDV) and sheeppox virus (SPV) have a considerable economic impact on the cattle and small ruminant industry. They are listed in group A of contagious disea ...
International Agency for Research on Cancer (IARC)
... aflatoxin B1 to form DNA adducts in vitro provide evidence that humans have the biochemical pathways required for aflatoxin-induced carcinogenesis. The following evidence is consistent with those biochemical mechanisms. Studies with bacteria show that activated aflatoxin B1 specifically induces G to ...
... aflatoxin B1 to form DNA adducts in vitro provide evidence that humans have the biochemical pathways required for aflatoxin-induced carcinogenesis. The following evidence is consistent with those biochemical mechanisms. Studies with bacteria show that activated aflatoxin B1 specifically induces G to ...
Parallel Session Abstracts
... Over the past decade, DNA barcoding has transitioned from academic battleground to major research enterprise. For the first few years, reviewers were unkind, critics were vocal, and funding was scarce. The situation has shifted; our community now oversees the largest research program ever undertaken ...
... Over the past decade, DNA barcoding has transitioned from academic battleground to major research enterprise. For the first few years, reviewers were unkind, critics were vocal, and funding was scarce. The situation has shifted; our community now oversees the largest research program ever undertaken ...
Molecular cloning of a laccase isozyme gene from
... which is typically unstable and may undergo a second enzyme-catalysed oxidation or a non-enzymic reaction such as hydration or spontaneous disproportionation and\or may partake in a polymerization reaction (Thurston, 1994). Laccases are members of the blue copper oxidase enzyme family that typically ...
... which is typically unstable and may undergo a second enzyme-catalysed oxidation or a non-enzymic reaction such as hydration or spontaneous disproportionation and\or may partake in a polymerization reaction (Thurston, 1994). Laccases are members of the blue copper oxidase enzyme family that typically ...
Understanding Reads in RNA-Seq Analysis
... exonic, the read is labeled “exonic”, and the same goes for partially overlaps exon, intronic and intergenic. In the other words, exonic takes precedence over partially overlaps exon, partially overlaps exon takes precedence over intronic, and intronic takes precedence over intergenic. Exonic: A rea ...
... exonic, the read is labeled “exonic”, and the same goes for partially overlaps exon, intronic and intergenic. In the other words, exonic takes precedence over partially overlaps exon, partially overlaps exon takes precedence over intronic, and intronic takes precedence over intergenic. Exonic: A rea ...
Selecting Degenerate Multiplex PCR Primers
... It is important to note that the degeneracy of a given _ -primer increases or remains the same, with the addition of additional sequence fragments. This observation permits us to employ a strategy which ignores degenerate primers with high degeneracy, in order to speed up the algorithm. Therefore, t ...
... It is important to note that the degeneracy of a given _ -primer increases or remains the same, with the addition of additional sequence fragments. This observation permits us to employ a strategy which ignores degenerate primers with high degeneracy, in order to speed up the algorithm. Therefore, t ...
TaqMan-Based Real-Time PCR for Genotyping Common
... frequencies of the HP1 and HP2 genotypes vary worldwide depending on the ethnic group, with the HP1 frequency varying from about 0.07 in parts of India to ⬎0.7 in parts of West Africa and South America (3 ). Because of its polymorphic nature, HP has been used as a genetic marker for identifying pare ...
... frequencies of the HP1 and HP2 genotypes vary worldwide depending on the ethnic group, with the HP1 frequency varying from about 0.07 in parts of India to ⬎0.7 in parts of West Africa and South America (3 ). Because of its polymorphic nature, HP has been used as a genetic marker for identifying pare ...
A survey on computational methods for enhancer and
... also depends on factors other than the sequence, sequence motifs can be considered a weak feature for enhancer prediction. As discussed above, using these static features to predict enhancers could at best identify regions with a potential to be an enhancer, without telling the contexts in which the ...
... also depends on factors other than the sequence, sequence motifs can be considered a weak feature for enhancer prediction. As discussed above, using these static features to predict enhancers could at best identify regions with a potential to be an enhancer, without telling the contexts in which the ...
What is p53
... cancers!! The function of p53 is critical to the way that many cancer treatments kill cells since radiotherapy and chemotherapy act in part by triggering cell suicide in response to DNA damage. This successful response to therapy is greatly reduced in tumours where p53 is mutant so these tumours are ...
... cancers!! The function of p53 is critical to the way that many cancer treatments kill cells since radiotherapy and chemotherapy act in part by triggering cell suicide in response to DNA damage. This successful response to therapy is greatly reduced in tumours where p53 is mutant so these tumours are ...
CHUKRASIA TABULARIS OXIDATIVE STRESS Research Article
... species (ROS) and reactive nitrogen species (RNS) [1]. These free radicals, when present in balanced conditions, serve as signalling and regulatory molecules at physiological levels [2]. It has been anticipated over the past three decades that overproduction of free radicals causes metabolic and mor ...
... species (ROS) and reactive nitrogen species (RNS) [1]. These free radicals, when present in balanced conditions, serve as signalling and regulatory molecules at physiological levels [2]. It has been anticipated over the past three decades that overproduction of free radicals causes metabolic and mor ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... studies on effects of gamma-radiation on DNA structure. Around this time it had just been determined that DNA was the hereditary (genetic) material, and the fact that DNA is the molecular bases of heredity peaked Peacocke’s interest in this molecule (Drysdale and Peacocke 1961), although exactly how ...
... studies on effects of gamma-radiation on DNA structure. Around this time it had just been determined that DNA was the hereditary (genetic) material, and the fact that DNA is the molecular bases of heredity peaked Peacocke’s interest in this molecule (Drysdale and Peacocke 1961), although exactly how ...
Analysis of the Molecular Basis of Flowering Time Variation in
... our conditions as had been reported previously (Karlsson et al., 1993; Nordborg and Bergelson, 1999), but the F1 plants from the crosses to Col flowered even later, suggesting that Kondara and Kz-9 FRI alleles are fully functional and confer very late flowering in the presence of the strong Col FLC ...
... our conditions as had been reported previously (Karlsson et al., 1993; Nordborg and Bergelson, 1999), but the F1 plants from the crosses to Col flowered even later, suggesting that Kondara and Kz-9 FRI alleles are fully functional and confer very late flowering in the presence of the strong Col FLC ...
Critical Factors for Successful Real-Time PCR
... cDNA using a reverse transcriptase. Reverse transcriptases are enzymes generally derived from RNA-containing retroviruses. RT-PCR can take place in a two-step or one-step reaction. With two-step RT-PCR, the RNA is first reverse transcribed into cDNA using oligo-dT primers, random oligomers, or gene- ...
... cDNA using a reverse transcriptase. Reverse transcriptases are enzymes generally derived from RNA-containing retroviruses. RT-PCR can take place in a two-step or one-step reaction. With two-step RT-PCR, the RNA is first reverse transcribed into cDNA using oligo-dT primers, random oligomers, or gene- ...