Real-time PCR Handbook
... can withstand is typically used (usually 95°C). The denaturation time can be increased if template GC content is high. 2. Annealing: During annealing, complementary sequences have an opportunity to hybridize, so an appropriate temperature is used that is based on the calculated melting temperature ...
... can withstand is typically used (usually 95°C). The denaturation time can be increased if template GC content is high. 2. Annealing: During annealing, complementary sequences have an opportunity to hybridize, so an appropriate temperature is used that is based on the calculated melting temperature ...
Additional file 1
... ARNT, Myc and RTG3, whose consensus of DNA binding sites recorded in their PWMs is “cacgtg”. Therefore, we initially assign “cacgtg” as the DNA binding sites of bh2b. The same search is performed on Ipmyb1, but no clear consensus can be draw from Jaspar database. The following search is based on the ...
... ARNT, Myc and RTG3, whose consensus of DNA binding sites recorded in their PWMs is “cacgtg”. Therefore, we initially assign “cacgtg” as the DNA binding sites of bh2b. The same search is performed on Ipmyb1, but no clear consensus can be draw from Jaspar database. The following search is based on the ...
Peptide nucleic acids 2
... Peptide nucleic acids were first described by Nielsen et al. in 1991.12 It was shown that replacement of the natural deoxyribose phosphate backbone of DNA by repeating N-(2-aminoethyl)glycine units with the ...
... Peptide nucleic acids were first described by Nielsen et al. in 1991.12 It was shown that replacement of the natural deoxyribose phosphate backbone of DNA by repeating N-(2-aminoethyl)glycine units with the ...
Transvection Is Common Throughout the Drosophila
... larval ventral nervous system. (A) Schematic of a CRMGAL4 transgene inserted into an attP site. Red-shaded boxes indicate sequences that originate from the attBcontaining CRM-GAL4 vector. Yellow-shaded boxes indicate sequences from the attP-containing P-element. Gray boxes indicate the surrounding g ...
... larval ventral nervous system. (A) Schematic of a CRMGAL4 transgene inserted into an attP site. Red-shaded boxes indicate sequences that originate from the attBcontaining CRM-GAL4 vector. Yellow-shaded boxes indicate sequences from the attP-containing P-element. Gray boxes indicate the surrounding g ...
Creating order from chaos: epigenome dynamics in
... Lizarazu et al., 1996). However, fluorescence in situ hybridization with maize repetitive ...
... Lizarazu et al., 1996). However, fluorescence in situ hybridization with maize repetitive ...
Virulence factors of Salmonella enterica serovar Enteritidis
... 61). Once ingested, the bacteria first need to pass the acidic environment of the stomach and thus bacterial resistance against these acidic conditions plays an important role in infection (23). Upon reaching the alimentary tract of the chicken, the bacteria are able to colonize the gut without caus ...
... 61). Once ingested, the bacteria first need to pass the acidic environment of the stomach and thus bacterial resistance against these acidic conditions plays an important role in infection (23). Upon reaching the alimentary tract of the chicken, the bacteria are able to colonize the gut without caus ...
ABO genotyping in leukemia patients reveals new ABO variant alleles
... individuals into four blood groups (A, B, O, and AB). Numerous phenotypes with weak expression of A and B on red cells have been detected and different terminologies have been adopted for ABO subgroup classification (Issitt and Anstee, 1998). The sequence of nucleotides comprising the ABO gene, its ...
... individuals into four blood groups (A, B, O, and AB). Numerous phenotypes with weak expression of A and B on red cells have been detected and different terminologies have been adopted for ABO subgroup classification (Issitt and Anstee, 1998). The sequence of nucleotides comprising the ABO gene, its ...
Endonucleolytic processing of CCAless tRNA precursors by RNase
... in vivo, none of which have a CCA motif encoded in their genes, and YqjK cleaves tRNA precursors with the same speci®city as plant RNase Z in vitro. We have thus renamed the gene rnz. A CCA motif downstream of the discriminator base inhibits RNase Z activity in vitro, with most of the inhibition due ...
... in vivo, none of which have a CCA motif encoded in their genes, and YqjK cleaves tRNA precursors with the same speci®city as plant RNase Z in vitro. We have thus renamed the gene rnz. A CCA motif downstream of the discriminator base inhibits RNase Z activity in vitro, with most of the inhibition due ...
GFF files
... GFF files On common way for the annotations to be uploaded to annotation servers is through GFF files. GFF (‘Gene-Finding Format’ or ‘General Feature Format’). GFF is a format for describing genes and other features associated with DNA, RNA and protein sequences. It was firstly developed at the Sang ...
... GFF files On common way for the annotations to be uploaded to annotation servers is through GFF files. GFF (‘Gene-Finding Format’ or ‘General Feature Format’). GFF is a format for describing genes and other features associated with DNA, RNA and protein sequences. It was firstly developed at the Sang ...
Methods Five 1536-SNP GoldenGate assays (Fig. 1) Three pilot
... followed by the position of the SNP. For example, 3897-578 is a SNP at position 578 in the consensus sequence of assembly #32 unigene 3897. Assembly #32 contained 351,645 sequences from 267,439 clones and was made using CAP3 [37] with parameter settings p = 75, d = 240, f = 250, h = 90. These relat ...
... followed by the position of the SNP. For example, 3897-578 is a SNP at position 578 in the consensus sequence of assembly #32 unigene 3897. Assembly #32 contained 351,645 sequences from 267,439 clones and was made using CAP3 [37] with parameter settings p = 75, d = 240, f = 250, h = 90. These relat ...
Ret/PTC3 is the most frequent form of gene rearrangement
... papillary thyroid carcinomas only for ret/PTC1 rearrangements and found no rearrangement; Ishizaka et al. (1991) found one ret/PTC1 rearrangement among 11 carcinomas, and Wajjwalku et al. (1992) found only one such alteration among 38 carcinomas. On the basis of those observations, RET gene rearrang ...
... papillary thyroid carcinomas only for ret/PTC1 rearrangements and found no rearrangement; Ishizaka et al. (1991) found one ret/PTC1 rearrangement among 11 carcinomas, and Wajjwalku et al. (1992) found only one such alteration among 38 carcinomas. On the basis of those observations, RET gene rearrang ...
In Memoriam: Jacques Monod (1910–1976)
... into the bacteria, was born. Several years later, a galactoside transacetylase (coded by the a gene) and induced at the same time as b-galactosidase and permease, was discovered, thus facilitating later studies on the genetic determinism of induction (Zabin et al. 1962). The physiological function o ...
... into the bacteria, was born. Several years later, a galactoside transacetylase (coded by the a gene) and induced at the same time as b-galactosidase and permease, was discovered, thus facilitating later studies on the genetic determinism of induction (Zabin et al. 1962). The physiological function o ...
Gene Section MLL (myeloid/lymphoid or mixed lineage leukemia) Atlas of Genetics and Cytogenetics
... Local order: telomeric to PLZF, centromeric from RCK. ...
... Local order: telomeric to PLZF, centromeric from RCK. ...
Microscopic Mechanics of Hairpin DNA Translocation through
... large persistence length (~50 nm), the double helix of 10 basepairs was modeled to a good approximation as a rigid cylinder with a length of 3.4 nm, which was attached to one end of the freely jointed chain. A Markov chain of states was generated from a random initial conformation by the Metropolis ...
... large persistence length (~50 nm), the double helix of 10 basepairs was modeled to a good approximation as a rigid cylinder with a length of 3.4 nm, which was attached to one end of the freely jointed chain. A Markov chain of states was generated from a random initial conformation by the Metropolis ...
HIV-1 Reverse Transcriptase-associated
... competition with the natural substrate. Two active site RNase H inhibitors are studied in detail, β-thujaplicinol and GSK5750. Both bind to the RNase H active site through a metal ion-chelating mechanism; however neither is capable of accessing their binding sites in the presence of a pre-formed enz ...
... competition with the natural substrate. Two active site RNase H inhibitors are studied in detail, β-thujaplicinol and GSK5750. Both bind to the RNase H active site through a metal ion-chelating mechanism; however neither is capable of accessing their binding sites in the presence of a pre-formed enz ...
Persistence and dispersion of Acinetobacter spp. in the urban water
... other similar bacteria (Bergogne-Berezin & Towner, 1996). Bacteria of the genus Acinetobacter are found in many environments, including water, soil, sewage and food products. At least 0.001% of the total culturable, heterotrophic, aerobic bacterial population in water and soil is estimated to be Aci ...
... other similar bacteria (Bergogne-Berezin & Towner, 1996). Bacteria of the genus Acinetobacter are found in many environments, including water, soil, sewage and food products. At least 0.001% of the total culturable, heterotrophic, aerobic bacterial population in water and soil is estimated to be Aci ...
Document
... 10. A DNA nucleotide is composed of three parts. These three parts may be: a. phosphate, adenine, and thymine b. phosphate, deoxyribose, and thymine * c. phosphate, glucose, and cytosine d. adenine, thymine, and cytosine ...
... 10. A DNA nucleotide is composed of three parts. These three parts may be: a. phosphate, adenine, and thymine b. phosphate, deoxyribose, and thymine * c. phosphate, glucose, and cytosine d. adenine, thymine, and cytosine ...
MyGene.info Documentation
... common species (human, mouse, rat, fruitfly, nematode, zebrafish, thale-cress, frog and pig). All other species, you can provide their taxonomy ids. See more details here. Multiple species can be passed using comma as a separator. Passing “all” will query against all available species. Default: huma ...
... common species (human, mouse, rat, fruitfly, nematode, zebrafish, thale-cress, frog and pig). All other species, you can provide their taxonomy ids. See more details here. Multiple species can be passed using comma as a separator. Passing “all” will query against all available species. Default: huma ...
Asymmetries in Retrieval of Gene Function Information
... of the source followed by a slash followed by an accession number and can be searched with one or both components, e.g., genbank [si], AF001892 [si], genbank/AF001892 [si]. The SI field and the Entrez sequence database links are not linked. The PubMed links to these databases are created from the re ...
... of the source followed by a slash followed by an accession number and can be searched with one or both components, e.g., genbank [si], AF001892 [si], genbank/AF001892 [si]. The SI field and the Entrez sequence database links are not linked. The PubMed links to these databases are created from the re ...
A General Model of Codon Bias Due to GC Mutational Bias
... patterns of codons used without recourse to the added complexity of including non-synonymous mutations. Consider the codons relating to any particular amino acid. For any two codons X and Y that differ by a single nucleotide, we assume that the rates of mutation from X to Y and from Y to X are the s ...
... patterns of codons used without recourse to the added complexity of including non-synonymous mutations. Consider the codons relating to any particular amino acid. For any two codons X and Y that differ by a single nucleotide, we assume that the rates of mutation from X to Y and from Y to X are the s ...
Metatranscriptome_Pipeline_Tutorial.doc
... the use of the pipeline by going through the various steps to illustrate the complexity of the process and the underlying tools and files used and generated by the pipeline. To illustrate the process we are going to use sequence reads generated from the rumen of a cow. These are 100bp paired end rea ...
... the use of the pipeline by going through the various steps to illustrate the complexity of the process and the underlying tools and files used and generated by the pipeline. To illustrate the process we are going to use sequence reads generated from the rumen of a cow. These are 100bp paired end rea ...
Optimal estimation of diffusion coefficients from single
... derived based on the much simpler statistics of the singletime-lapse displacements [19,20]. This estimator is known to be optimal, asymptotically, in the limit of infinitely long time series. There it is unbiased and as precise as is possible: Its variance is as small as the Cramér-Rao bound [18]. ...
... derived based on the much simpler statistics of the singletime-lapse displacements [19,20]. This estimator is known to be optimal, asymptotically, in the limit of infinitely long time series. There it is unbiased and as precise as is possible: Its variance is as small as the Cramér-Rao bound [18]. ...
Methods for Improving Aptamer Binding Affinity
... conducted a few truncations on the stem-loop regions to understand the significance of stem-loop regions, and one of the truncated aptamers showed a significant increase in the binding affinity for the target [16]. Shangguan and coworkers identified binding sites of selected aptamers by sequence tru ...
... conducted a few truncations on the stem-loop regions to understand the significance of stem-loop regions, and one of the truncated aptamers showed a significant increase in the binding affinity for the target [16]. Shangguan and coworkers identified binding sites of selected aptamers by sequence tru ...
Crimes Act 1914 - Federal Register of Legislation
... judges, magistrates or other court employed officers a function or power that is neither judicial nor incidental to a judicial function or power. For example, the power of a magistrate to order the carrying out of a forensic procedure on a suspect under section 23WS of the Crimes Act 1914. The propo ...
... judges, magistrates or other court employed officers a function or power that is neither judicial nor incidental to a judicial function or power. For example, the power of a magistrate to order the carrying out of a forensic procedure on a suspect under section 23WS of the Crimes Act 1914. The propo ...
34386 - PubAg
... Volicitin Induction of Igl Expression Is Fast and Transient. The kinetics of Igl induction were investigated by Northern blot analysis with the bx1bx1 mutant maize line. GAP C, a gene not inf luenced by volicitin treatment (Fig. 2), was used to normalize for loading differences on the gels. The low ...
... Volicitin Induction of Igl Expression Is Fast and Transient. The kinetics of Igl induction were investigated by Northern blot analysis with the bx1bx1 mutant maize line. GAP C, a gene not inf luenced by volicitin treatment (Fig. 2), was used to normalize for loading differences on the gels. The low ...