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Examining the Gm18 and m G Modification Positions in tRNA
Examining the Gm18 and m G Modification Positions in tRNA

... RNA, and in tRNA alone 92 such modifications have been recorded [12]. Prominent among those are 2'-OH ribose methylated nucleoside, which is commonly found in tRNAs. The posttranscriptional modification in RNA is mediated through specific methyltransferase enzymes [13]. These modifications can occur ...
VAAST 2 User`s Guide
VAAST 2 User`s Guide

... VAAST,  the  Variant  Annotation,  Analysis  and  Search  Tool  is  a  probabilistic  search   tool  for  identifying  damaged  genes  and  their  disease-­‐causing  variants  in  personal   genome  sequences.  VAAST  builds  upon  existing   ...
Attachment 2.2 Sequencing results
Attachment 2.2 Sequencing results

... On the other hand, AHR deficient mice have a reduced body weight, display reduced fecundity and are defective in development of both liver and immune system. In these mice fetal vascular structures are found in the eyes, kidneys and liver. [1] Objective: to study the conservation of the AHR gene and ...
Rfam Documentation
Rfam Documentation

Genetics Practice Test
Genetics Practice Test

... 80. Some plasmids have genes that make bacteria resistant to ____________________ and allow them serve as genetic markers. ...
Rapid Microscope Based Identification Method for Tuberculosis and
Rapid Microscope Based Identification Method for Tuberculosis and

... mycobacteria, they are laborious and time-consuming. Fortunately, several improvements have been made. For example, the recently developed culture methods based on radiometric BACTEC and non-radiometric Mycobacteria Growth Indicator Tube (MGIT) have reduced the time for the identification from weeks ...
Creating order from chaos: epigenome dynamics in
Creating order from chaos: epigenome dynamics in

... We hypothesize that the current structure of a genome is the result of various TE bursts ...
Specificity of Insertion by the Translocatable Tetracycline Resistance Element Tn10.
Specificity of Insertion by the Translocatable Tetracycline Resistance Element Tn10.

... Genetic analysis of 131 independent transpositions of the tetracyclineresistance element TnlO from a single site in phage P22 into the histidine operon of Salmonella typhimurium reveals that TnZO insertions are not randomly distributed along this chromosomal target. The insertions occur in 22 differ ...
Unipro UGENE Manual
Unipro UGENE Manual

... You're going to analyze ChIP-Seq data with the Cistrome pipeline Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched from the UGENE graphical interface, an external tool needs a corresponding executable file. The list of the external tools can be found on thi ...
Unipro UGENE Manual
Unipro UGENE Manual

... You're going to analyze ChIP-Seq data with the Cistrome pipeline Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched from the UGENE graphical interface, an external tool needs a corresponding executable file. The list of the external tools can be found on thi ...
Asymmetries in Retrieval of Gene Function Information
Asymmetries in Retrieval of Gene Function Information

... of the source followed by a slash followed by an accession number and can be searched with one or both components, e.g., genbank [si], AF001892 [si], genbank/AF001892 [si]. The SI field and the Entrez sequence database links are not linked. The PubMed links to these databases are created from the re ...
A family of thermostable fungal cellulases created by structure-guided recombination
A family of thermostable fungal cellulases created by structure-guided recombination

... of contiguous amino acid ‘‘blocks’’ that minimize 具E典, the library average number of amino acid sidechain contacts that are broken when the blocks are swapped among different parents. Meyer et al. (4) found that the probability that a ␤-lactamase chimera was folded and active was inversely related t ...
Expression of a Maize Sucrose Phosphate Synthase
Expression of a Maize Sucrose Phosphate Synthase

... is used for initiation). The derived d068-amino acid polypeptide contained all 5 peptide sequences obtained by direct protein sequencing of the SPS30 and SPS90 tryptic peptides(Figure 1). This confirmed our immunological data that had indicated that SPS30 and SPS90 had arisen from 1 polypeptide. In ...
Product description P018-G1 SHOX-v03 - MRC
Product description P018-G1 SHOX-v03 - MRC

... SHOX gene function. - Please note that single exon deletions have a considerable chance of being a false positive result, either due to non-pathogenic copy number variants (Benito-Sanz et al. 2011a), mutations within the probe binding site (Barca-Tierno et al. 2011) or due to impurities in the DNA. ...
PROGRAM PROGRAM
PROGRAM PROGRAM

... plant growth control (R. Archacki) – 30 min • HD2C histone deacetylase and a SWI/SNF complex are both involved in mediating the heat stress response in Arabidopsis (D. Buszewicz) – 15 min • The evolutionarily conserved histone acetylatransferase complex NuA4 is crucial for plant development (T. Bi ...
Virulence factors of Salmonella enterica serovar Enteritidis
Virulence factors of Salmonella enterica serovar Enteritidis

... humans, the infection can manifest as a non-bloody diarrhea with abdominal pain, nausea, vomiting and fever. The disease (non-typhoidal fever) is usually self-limiting and recovery follows within a few days to a week but, occasionally, systemic infection may occur in vulnerable human patients such a ...
Resistance to hydrogen peroxide in Helicobacter pylori
Resistance to hydrogen peroxide in Helicobacter pylori

... HP0874 are transcribed independently. However, there are no recognizable RNA polymerase binding sites upstream of HP0874 (Manos, 1998 ; Odenbreit et al., 1996). Using a yeast two-hybrid model, Rain et al. (2001) have indicated that the protein encoded by HP0874 interacts with KatA. This possible int ...
ABO genotyping in leukemia patients reveals new ABO variant alleles
ABO genotyping in leukemia patients reveals new ABO variant alleles

... The sequence of nucleotides comprising the ABO gene, its structure and some of its common variants were determined in the early 1990s (Yamamoto and Hakomori, 1990; Yamamoto et al., 1990a). This gene, mapped on chromosome 9q34.1-34.2, consists of 7 exons ranging from 28 to 688 bp. More than 90% of th ...
Real-time PCR-based detection of Bordetella pertussis and
Real-time PCR-based detection of Bordetella pertussis and

In Memoriam: Jacques Monod (1910–1976)
In Memoriam: Jacques Monod (1910–1976)

... E. coli. Later, among a number of mutants that had been isolated, several seemed to be lactose and were yet able to synthesize b-galactosidase. The explanation for such mysterious mutants, referred to as ‘‘cryptic,’’ was found in 1956: such mutants were lacking a specific protein, which, in wildtyp ...
Epigenetic Interactions among Three dTph1 Transposons in Two
Epigenetic Interactions among Three dTph1 Transposons in Two

... elements of maize are thought to transpose by a (quasi)replicative mechanism in germinal cells, and the structure of Mu excision products fits with a DSB repair model (Lisch et al., 1995; Hsia and Schnable, 1996; Mathern and Hake, 1997). Even though reversion of Ac insertion alleles in maize does no ...
Epigenetic Interactions among Three dTph1
Epigenetic Interactions among Three dTph1

... elements of maize are thought to transpose by a (quasi)replicative mechanism in germinal cells, and the structure of Mu excision products fits with a DSB repair model (Lisch et al., 1995; Hsia and Schnable, 1996; Mathern and Hake, 1997). Even though reversion of Ac insertion alleles in maize does no ...
Barley genetic variation: implications for crop improvement
Barley genetic variation: implications for crop improvement

... Genetic variation is crucial for successful barley improvement. Genomic technologies are improving dramatically and are providing access to the genetic diversity within this important crop species. Diverse collections of barley germplasm are being assembled and mined via genome-wide association stud ...
Transvection Is Common Throughout the Drosophila
Transvection Is Common Throughout the Drosophila

... Figure 1 Independent labeling of two hemilineages in the larval ventral nervous system. (A) Schematic of a CRMGAL4 transgene inserted into an attP site. Red-shaded boxes indicate sequences that originate from the attBcontaining CRM-GAL4 vector. Yellow-shaded boxes indicate sequences from the attP-c ...
TaqMan-Based Real-Time PCR for Genotyping Common
TaqMan-Based Real-Time PCR for Genotyping Common

... and HP2 for typing (14, 15 ). More recently, a singlechain antibody-based ELISA test was also established (16 ). In addition, several HP-genotyping methods based on conventional PCR have been developed; however, these PCR-based methods need to amplify a relatively large DNA fragment to detect the 1. ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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