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RNA Editing, ADAR1, and the Innate Immune Response
RNA Editing, ADAR1, and the Innate Immune Response

... ADAR1 was originally thought to be the enzyme responsible for GluR-B mRNA editing [41,42] and through this function significantly affect neurological functions [43–45]. However, this important editing was subsequently attributed to ADAR2 [24]. ADAR1 indeed participates in the editing of many other s ...
A survey on computational methods for enhancer and
A survey on computational methods for enhancer and

... also depends on factors other than the sequence, sequence motifs can be considered a weak feature for enhancer prediction. As discussed above, using these static features to predict enhancers could at best identify regions with a potential to be an enhancer, without telling the contexts in which the ...
Chromosomes Carrying Meiotic Avoidance Loci
Chromosomes Carrying Meiotic Avoidance Loci

... et al., 2004; Conner et al., 2008). By contrast, two independent dominant loci control apomixis in Hieracium praealtum. Genetic markers linked to these loci have been determined using a deletion mapping approach (Catanach et al., 2006). The roles of these two loci and their interactions with the sex ...
Rh phenotype prediction by DNA typing and its
Rh phenotype prediction by DNA typing and its

Selection for TnlO Tet Repressor Binding to tet Operator
Selection for TnlO Tet Repressor Binding to tet Operator

... at 42” and white at 28 O with frequencies ranging from 2 to85%. The tetR genes from onecandidate of each of the sevenselectionswere sequenced. The obtained mutations are displayed in Figure 3. Temperature-sensitive Tet repressor mutants contained either a glycine to glutamic acid exchange at positio ...
Phylogenetic analysis of three complete gap
Phylogenetic analysis of three complete gap

... from the zebrafish genome, some are found as single relatives, and others are found in multiple copies. In addition, the zebrafish has 14 apparently novel connexins, several of which arose by recent tandem duplication events. This analysis provides evidence that the connexin gene family is increasin ...
Molecular Identification of Vibrio harveyi From Larval Stage of
Molecular Identification of Vibrio harveyi From Larval Stage of

`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and

... Apple proliferation (AP), pear decline (PD) and European stone fruit yellows (ESFY) are among the most economically important plant diseases that are caused by phytoplasmas. Phylogenetic analyses revealed that the 16S rDNA sequences of strains of each of these pathogens were identical or nearly iden ...
A MIAME-compliant Microarray Database
A MIAME-compliant Microarray Database

... International Transcriptomic Projects • Many places, like NASC, have been funded to do run a lot of microarrays • If you are going to run lots of microarrayswhy not form a coordinated project to use all of them? • Creation of coordinated transcriptome ...
Human mutations in glucose 6-phosphate dehydrogenase reflect
Human mutations in glucose 6-phosphate dehydrogenase reflect

... evolutionary history. FASEB J. 14, 485– 494 (2000) ...
A Comparative Study on the Recovery of EcoRI Endonuclease from
A Comparative Study on the Recovery of EcoRI Endonuclease from

... Restriction enzymes have extensive applications in recombinant DNA technology. They are used in the preparation of recombinant molecules, and they provide an attractive system for the analysis of sequence specific DNA-protein interactions1 . Escherichia coli RI (EcoRI) endonuclease is a well-known r ...
The Rad50 Signature Motif: Essential to ATP Binding and
The Rad50 Signature Motif: Essential to ATP Binding and

... The repair of double-strand breaks in DNA is an essential process in all organisms, and requires the coordinated activities of evolutionarily conserved protein assemblies. One of the most critical of these is the Mre11/ Rad50 (M/R) complex, which is present in all three biological kingdoms, but is n ...
Control of DNA excision efficiency in Paramecium
Control of DNA excision efficiency in Paramecium

... alleles has demonstrated that the 5′-TA-3′ dinucleotide invariably flanking IES ends is strictly required for IES excision. A mutation in the 5′-TA-3′ dinucleotide is associated with either defective excision of the mutated IES (6) or a shift of the IES end towards the next two 5′-TA-3′ dinucleotide ...
List of references - UC Davis Plant Sciences
List of references - UC Davis Plant Sciences

... Kawagoe, Y. and U. Kikuta. 1991. Chloroplast DNA evolution in potato (Solanum tuberosum L.). TAG 81:13-20. Kemble, R.J. et al. 1986. Mitochondrial DNA rearrangements in somatic hybrids of Solanum tuberosum and S. brevidens. TAG 72:787-793. Kidane-Marian, H.M. et al. 1985. Comparisons between 4X x 2X ...
Telomere maintenance without telomerase
Telomere maintenance without telomerase

... ability to grow but also displayed dramatic changes at their chromosomal termini, due to global ampli®cation of both telomeric and sub-telomeric repeat sequences. These extensive rearrangements arose as a result of recombination, as the appearance of survivors was blocked if the est1-D strain was al ...
Introduction to GO Annotation
Introduction to GO Annotation

... In this study, we report the isolation and molecular characterization of the B. napus PERK1 cDNA, that is predicted to encode a novel receptor-like kinase. We have shown that like other plant RLKs, the kinase domain of PERK1 has serine/threonine kinase activity, In addition, the location of a PERK1- ...
illuminaHumanv3.db October 28, 2014
illuminaHumanv3.db October 28, 2014

... indicates the chromosome. Due to inconsistencies that may exist at the time the object was built, these vectors may contain more than one chromosome and/or location. If the chromosomal location is unknown, the vector will contain an NA. Chromosomal locations on both the sense and antisense strands a ...
Gene Section MLL (myeloid/lymphoid or
Gene Section MLL (myeloid/lymphoid or

... Transcription ...
Maintenance of genomic integrity by p53: complementary
Maintenance of genomic integrity by p53: complementary

... repair processes, despite some evidence pointing to this possibility. Last but not least, a possible role of p53 in the control of genomic integrity in its non-induced state, i.e., in the absence of signals indicating DNA damage or other potentially harmful conditions, so far has not yet been consid ...
Standards and guidelines for the interpretation of sequence
Standards and guidelines for the interpretation of sequence

... often lead to confusion because of incorrect assumptions of pathogenic and benign effects, respectively. Thus, it is recommended that both terms be replaced by the term “variant” with the following modifiers: (i) pathogenic, (ii) likely pathogenic, (iii) uncertain significance, (iv) likely benign, o ...
multicopy gene family evolution on primate y chromosomes
multicopy gene family evolution on primate y chromosomes

... Contrary to previous theories, the Y chromosome is an important determinant of male phenotype [15–17]. The Y chromosome has been found to be associated with male fertility in humans [18] and even less obvious traits like lifespan [19] and locomotive activity [20] in Drosophila species. Therefore, an ...
Evolutionary dynamics of nematode operons
Evolutionary dynamics of nematode operons

... 2006), and several models have been proposed to explain the prevalence of operons in prokaryotes (Lawrence and Roth 1996; Lawrence 1999). However, these models are not applicable to nematode operons. For example, the natal model claims that genes found in operons are in situ duplicates (Lawrence 199 ...
Bacteriophage resistance mechanisms
Bacteriophage resistance mechanisms

DNA
DNA

Primer on Molecular Biology
Primer on Molecular Biology

... More Terminology • The genome is an organism’s complete set of DNA. ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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