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Transcript
March 15, 2006
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Multiple choice questions
(numbers in brackets indicate the number of correct answers)
Insulators
Delimit functional domains
Delimit structural domains
Stimulate gene expression
are usually smaller than 1000 bp
overcome positional effects in gene expression
Locus control regions
Are located close to genes
Stimulate gene expression
Are usually smaller than 1000 bp
Contain Dnase I hypersensitive sites
Are present in prokaryotes
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Nuclear gene expression can be regulated by
Histone methylation
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Histone acetylation
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Histone remodeling
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Histone re-arrangement
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Histone glycosylation
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Histone deamination
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Genomic imprinting
Activates genes
Involves DNA methylation
Is only found in mammals
Inactivates one of the sex chromosomes
Has been found for hundreds of genes
Is always maternally inherited
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DNA-binding proteins
Are usually monomeric
Interact with DNA by ionic bonds
Contain DNA-binding motifs
Can regulate gene expression
Can be isolated by affinity chromatography
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Promoters
Are DNA sequences
Are always in front of genes
Consist of consensus sequences
Are binding sites for RNA polymerases
Are rich in G and C nucleotides
Are only found with protein-coding genes
Are very similar in prokaryotes and eukaryotes
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Enhancers
Are proteins
Are DNA sequences
Activate transcription
Are located next to a gene
Bind to RNA polymerase
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Transcription termination
Occurs at the ends of coding regions
Can be induced by specific RNA stem-loops
Is similar in prokaryotes and in the nucleus of eukaryotes
Can involve the action of several proteins
Is always linked to translation
Can be regulated
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Exons
Are always found in the same positions in homologous genes
Are always flanked by two introns
Are protein-coding sequences
Outnumber the introns in an RNA
Are common in ribosomal RNAs
Splicing
Removes introns
Removes exons
Always requires spliceosomes
Occurs primarily in the cytoplasm
May require more than 100 proteins
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