Download 國立嘉義大學九十七學年度

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Document related concepts

Nucleic acid analogue wikipedia, lookup

Deoxyribozyme wikipedia, lookup

Point mutation wikipedia, lookup

Artificial gene synthesis wikipedia, lookup

Cre-Lox recombination wikipedia, lookup

Gel electrophoresis of nucleic acids wikipedia, lookup

Polycomb Group Proteins and Cancer wikipedia, lookup

X-inactivation wikipedia, lookup

Genome (book) wikipedia, lookup

Microevolution wikipedia, lookup

Dominance (genetics) wikipedia, lookup

Designer baby wikipedia, lookup

Genomic imprinting wikipedia, lookup

Gene expression programming wikipedia, lookup

Epigenetics of human development wikipedia, lookup

Gene wikipedia, lookup

Nutriepigenomics wikipedia, lookup

Gene expression profiling wikipedia, lookup

Site-specific recombinase technology wikipedia, lookup

History of genetic engineering wikipedia, lookup

Genetic engineering wikipedia, lookup

Genome evolution wikipedia, lookup

RNA-Seq wikipedia, lookup

Gene nomenclature wikipedia, lookup

Primary transcript wikipedia, lookup

Therapeutic gene modulation wikipedia, lookup

NEDD9 wikipedia, lookup

Helitron (biology) wikipedia, lookup

Non-coding DNA wikipedia, lookup

Vectors in gene therapy wikipedia, lookup

Epigenetics in learning and memory wikipedia, lookup

Epigenetics of neurodegenerative diseases wikipedia, lookup

Epigenomics wikipedia, lookup

Cancer epigenetics wikipedia, lookup

Epigenetics of diabetes Type 2 wikipedia, lookup

Nucleosome wikipedia, lookup

DNA vaccination wikipedia, lookup

Genomics wikipedia, lookup

Extrachromosomal DNA wikipedia, lookup

Cell-free fetal DNA wikipedia, lookup

DNA supercoil wikipedia, lookup

Gene therapy wikipedia, lookup

Human genome wikipedia, lookup

No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia, lookup

Genome editing wikipedia, lookup

Genomic library wikipedia, lookup

Zinc finger nuclease wikipedia, lookup

Molecular cloning wikipedia, lookup

Nucleic acid double helix wikipedia, lookup

DNA damage theory of aging wikipedia, lookup

Oncogenomics wikipedia, lookup

SNP genotyping wikipedia, lookup

Transcript
國立嘉義大學 99 學年度
生化科技學系碩士班招生考試試題
科目:分子生物學
3. (1) On chromosome 22 (4.8 x 107 base pairs), there are about 700 genes. If the average size of the
genes is 19,000 base pairs, each gene on average contains 5.4 exons, and the average size of the
1. Define the following terms: (25%)
(1) Holliday junction
(2) Klenow fragment
(3) Okazaki fragment
(4) Kozak’s rules
(5) Shine-Dalgarno Sequence
2. Match each tem on the left with a statement on the right (25%)
Term
Statement
(1) Gene
a. Has no effect on phenotype in a heterozygote
(2) Allele
(3) Character
(4) Trait
(5) Recessive allele
(6) Genotype
(7) Phenotype
(8) Heterozygous
(9) Testcross
(10) Monohybrid cross
b. A variant for a character
c. A cross between individuals heterozygous for a single character
d. An alternative version of a gene
e. Having two different alleles for a gene
f. A heritable feature that varies among individuals
g. An organism's appearance or observable traits
h. A cross between an individual with an unknown genotype and a
homozygous recessives individual
i. The genetic makeup of an individual
j. A heritable unit that determines a character; can exist in different
forms
exons is 266 base pairs. (i) Calculate the fraction of nucleotides in a gene that will be present in
mRNA? (ii) What the fraction of the chromosome that is occupied by genes? (iii) Propose a simple
procedure that would help us to establish the intron/exon structure of a gene. (9%)
(2) How many different DNA fragments would you expect to obtain if you completely digest a bacterial
genomic DNA (4 x 106 nucleotide pairs) with HaeIII (4-base recognition site)? or with EcoR I (6base recognition site)? (6%)
(3) Which of the following statements are correct? For the incorrect statements, correct them
specifically (hint: the correction should not be simply from “can” to “cannot”, or from “is” to
“isn’t”). (10%)
(i) Restriction endonucleases cut DNA at specific sites that always located between genes.
(ii) DNA migrates toward the positive electrode during electrophoresis.
(iii) DNA ligase can join two DNA fragments with protruding end on one fragment and blunt end
on the other.
(iv) In situ hybridization can be used to locate nucleic acid sequences in cells or on chromosomes.
(v) DNA microarray is an application of restriction nuclease cleavage of DNA.
4. Anti-cancer Drug A could decrease protein X expression in breast cancer cells lines. Our hypothesis is
the protein X could be regulated by Drug A in the transcriptional levels and post-translational levels.
(1) Please describe experiments to verify the hypothesis. (10%)
(2) We assume protein X is an oncogene. Protein X is overexpressed in breast cancer. Give an example
for oncogene activation. (10%)
(3) Describe the techniques to knockdown Protein X expression. (5%)