Streptococcus faecium - International Journal of Systematic and
... durans”, and “Streptococcus casselifiavus”) had DNA G +C contents in the range from 37 to 43 mol%. These data are in good agreement with the data given recently by Farrow et al. (7); however, they are about 1to 2 mol% lower than the values reported by Kilpper-Balz et al. (12), who used a value for t ...
... durans”, and “Streptococcus casselifiavus”) had DNA G +C contents in the range from 37 to 43 mol%. These data are in good agreement with the data given recently by Farrow et al. (7); however, they are about 1to 2 mol% lower than the values reported by Kilpper-Balz et al. (12), who used a value for t ...
Unequal Crossing-over in Unique PABP2 Mutations in Japanese
... repeats are responsible for a number of hereditary neuromuscular diseases.7,14,15 These pathologic repeat expansions can be explained by the slippage model. However, it has been proposed that tracts of approximately 25 to 35 perfect trinucleotide repeats are required for instability and expansion vi ...
... repeats are responsible for a number of hereditary neuromuscular diseases.7,14,15 These pathologic repeat expansions can be explained by the slippage model. However, it has been proposed that tracts of approximately 25 to 35 perfect trinucleotide repeats are required for instability and expansion vi ...
Spinocerebellar ataxia type 1 (SCA1): Phenotype-genotype
... 35 ± 38 triplets are interrupted by the sequence CAT CAG CAT. In alleles with 39 ± 41 triplets the interruption is missing in six of seven cases. DNA sequences are summarised in Table 1. Alleles with 35 ± 38 triplets are present in patients with ataxia but without additional characteristic features ...
... 35 ± 38 triplets are interrupted by the sequence CAT CAG CAT. In alleles with 39 ± 41 triplets the interruption is missing in six of seven cases. DNA sequences are summarised in Table 1. Alleles with 35 ± 38 triplets are present in patients with ataxia but without additional characteristic features ...
Journal of Applied Microbiology
... (Darrasse et al. 1994), were also used. PCR amplification was carried out as described but with the following thermal regime: 94C/10 min, 25x (94C/60 s, 67C/60 s, 72C/ 30 s) and 72C/10 min. Amplicons were resolved by agarose gel electrophoresis on a 1Æ5% agarose gel in TBE containing 2Æ5 ll ml) ...
... (Darrasse et al. 1994), were also used. PCR amplification was carried out as described but with the following thermal regime: 94C/10 min, 25x (94C/60 s, 67C/60 s, 72C/ 30 s) and 72C/10 min. Amplicons were resolved by agarose gel electrophoresis on a 1Æ5% agarose gel in TBE containing 2Æ5 ll ml) ...
Principles and Practice of Agarose Gel Electrophoresis
... Samples are prepared for electrophoresis by mixing them with components that will give the mixture density, such as glycerol or sucrose. This makes the samples denser than the electrophoresis buffer. These samples can then be loaded with a micropipet or transfer pipet into wells that were created in ...
... Samples are prepared for electrophoresis by mixing them with components that will give the mixture density, such as glycerol or sucrose. This makes the samples denser than the electrophoresis buffer. These samples can then be loaded with a micropipet or transfer pipet into wells that were created in ...
Rapid Microscope Based Identification Method for Tuberculosis and
... may be caused by several parameters, such as low rRNA content due to low metabolism, or weak permeabiliziation of the cell so that the probe cannot penetrate into the cell. To increase the permeability in general, different types of chemical-physical treatments or enzymes (e.g. lysozyme, mutanolysin ...
... may be caused by several parameters, such as low rRNA content due to low metabolism, or weak permeabiliziation of the cell so that the probe cannot penetrate into the cell. To increase the permeability in general, different types of chemical-physical treatments or enzymes (e.g. lysozyme, mutanolysin ...
Analysis of the genetic potential of the spongederived fungus
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
Population Genetics - University of Evansville Faculty Web sites
... There are duplicated noncoding regions (or repetitive regions) of the DNA referred to as miniand microsatellite sequences This DNA is similar among closely related organisms – that is, there is a core sequence of nucleotides shared among closely related individuals However, each individual also has ...
... There are duplicated noncoding regions (or repetitive regions) of the DNA referred to as miniand microsatellite sequences This DNA is similar among closely related organisms – that is, there is a core sequence of nucleotides shared among closely related individuals However, each individual also has ...
New variants of the tet(M) gene in Clostridium
... tet(M)1, 2, 3 and 4 variants were found in C. difficile isolates resistant or inducibly resistant to tetracycline, and tet(M)5 and 6 were found in susceptible isolates. The organization of the element carrying tet(M)3, tet(M)6 and tet(M)1 of cd12 was similar to that of E. faecalis DS16. This analysi ...
... tet(M)1, 2, 3 and 4 variants were found in C. difficile isolates resistant or inducibly resistant to tetracycline, and tet(M)5 and 6 were found in susceptible isolates. The organization of the element carrying tet(M)3, tet(M)6 and tet(M)1 of cd12 was similar to that of E. faecalis DS16. This analysi ...
1 FRQ-interacting RNA Helicase (FRH) Mediates Negative and
... a region with ~5 map units between markers SNP6 and SNP72 near centromere I (CENI) (Figure 3A). Unexpectedly however, two out of 10 recombinant progeny showed recombination with both SNP markers, suggesting either an exceptional frequency of double recombination, gene conversion, or the presence of ...
... a region with ~5 map units between markers SNP6 and SNP72 near centromere I (CENI) (Figure 3A). Unexpectedly however, two out of 10 recombinant progeny showed recombination with both SNP markers, suggesting either an exceptional frequency of double recombination, gene conversion, or the presence of ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... to note that the very dyes that were used in Peacocke’s pioneering studies with DNA structure are still used in most molecular biology laboratories today as dyes to detect DNA and RNA when they have been separated into different size fragments using a technique called gel electrophoresis. As Peacock ...
... to note that the very dyes that were used in Peacocke’s pioneering studies with DNA structure are still used in most molecular biology laboratories today as dyes to detect DNA and RNA when they have been separated into different size fragments using a technique called gel electrophoresis. As Peacock ...
FilmArray Blood Culture Panel Procedure in CLSI Template
... the 2nd stage PCR array. The individual wells of the array contain primers for different assays (each present in triplicate) that target specific nucleic acid sequences from each of the pathogens detected, as well as control template material. These primers are ‘nested’ or internal to the specific p ...
... the 2nd stage PCR array. The individual wells of the array contain primers for different assays (each present in triplicate) that target specific nucleic acid sequences from each of the pathogens detected, as well as control template material. These primers are ‘nested’ or internal to the specific p ...
Analysis of the Molecular Basis of Flowering Time Variation in
... major determinant of vernalization requirement and flowering time variation (Napp-Zinn, 1985; Burn et al., 1993; Lee et al., 1993; Clarke and Dean, 1994). FRI functions to increase RNA levels of the floral repressor FLC (FLOWERING LOCUS C), which represses the expression of genes required for the tr ...
... major determinant of vernalization requirement and flowering time variation (Napp-Zinn, 1985; Burn et al., 1993; Lee et al., 1993; Clarke and Dean, 1994). FRI functions to increase RNA levels of the floral repressor FLC (FLOWERING LOCUS C), which represses the expression of genes required for the tr ...
The Nicotiana tabacum genome encodes two cytoplasmic
... and Abel 1975). Preparation of a partial library and isolation of a genomic clone. DNA was digested with EcoRI and electrophoresed on a preparative 0.8% agarose gel at 35 V. Fragments were collected according to their size in five fractions : 2.7-4.0 kb, 3.4-4.9 kb, 3.7-9.0 kb, 5.0-9.5 kb and 6.5-23 ...
... and Abel 1975). Preparation of a partial library and isolation of a genomic clone. DNA was digested with EcoRI and electrophoresed on a preparative 0.8% agarose gel at 35 V. Fragments were collected according to their size in five fractions : 2.7-4.0 kb, 3.4-4.9 kb, 3.7-9.0 kb, 5.0-9.5 kb and 6.5-23 ...
Consistency analysis of redundant probe sets on Affymetrix three
... Expression arrays from Affymetrix contain hundreds of thousands of oligonucleotide probes grouped into functional units called probe sets, where each probe set is designed to measure the expression of a known or computationally-predicted mRNA target molecule. Affymetrix sells two commonly-used types ...
... Expression arrays from Affymetrix contain hundreds of thousands of oligonucleotide probes grouped into functional units called probe sets, where each probe set is designed to measure the expression of a known or computationally-predicted mRNA target molecule. Affymetrix sells two commonly-used types ...
POPULATION GENETICS – BIOL 101
... Attached Ear Lobe (ee): In many people, the ear lobes hang free, but when a person is homozygous recessive for an allele (ee), the ear lobes are attached directly on to the side of the head so that there is no lobe hanging freely. Widow's Peak (W): If the hairline drops downward and forms a distinct ...
... Attached Ear Lobe (ee): In many people, the ear lobes hang free, but when a person is homozygous recessive for an allele (ee), the ear lobes are attached directly on to the side of the head so that there is no lobe hanging freely. Widow's Peak (W): If the hairline drops downward and forms a distinct ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... acceptors on DNA, which was achieved by bending the DNA over spermine, a process that encases the polyamine in a deepened, narrowed, and highly electronegative major groove. Thus there are considerable data that support specific interactions of polyamines with nucleic acids. More importantly, when p ...
... acceptors on DNA, which was achieved by bending the DNA over spermine, a process that encases the polyamine in a deepened, narrowed, and highly electronegative major groove. Thus there are considerable data that support specific interactions of polyamines with nucleic acids. More importantly, when p ...
Single-stranded heteroduplex intermediates in l Red homologous
... [31,32,45], the host cells contained only Redb. In parallel as a positive control, we performed a recombination reaction with single-stranded oligonucleotides to repair a mutation in the kanamycin resistance gene (neo) and so restore kanamycin resistance. As expected from previous observations, the ...
... [31,32,45], the host cells contained only Redb. In parallel as a positive control, we performed a recombination reaction with single-stranded oligonucleotides to repair a mutation in the kanamycin resistance gene (neo) and so restore kanamycin resistance. As expected from previous observations, the ...
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
... Apple proliferation (AP), pear decline (PD) and European stone fruit yellows (ESFY) are among the most economically important plant diseases that are caused by phytoplasmas. Phylogenetic analyses revealed that the 16S rDNA sequences of strains of each of these pathogens were identical or nearly iden ...
... Apple proliferation (AP), pear decline (PD) and European stone fruit yellows (ESFY) are among the most economically important plant diseases that are caused by phytoplasmas. Phylogenetic analyses revealed that the 16S rDNA sequences of strains of each of these pathogens were identical or nearly iden ...
ABO genotyping in leukemia patients reveals new ABO variant alleles
... (DiaMed Latino America), polyclonal anti-A, anti-B, anti-AB (Fresenius Hemocare, SP, Brazil), and lectin anti-A1 and monoclonal anti-H (Gamma Biologicals, Houston, TX, USA). All reagents were used as recommended by the manufacturer’s instructions. ABO reverse typing was conducted by agglutination te ...
... (DiaMed Latino America), polyclonal anti-A, anti-B, anti-AB (Fresenius Hemocare, SP, Brazil), and lectin anti-A1 and monoclonal anti-H (Gamma Biologicals, Houston, TX, USA). All reagents were used as recommended by the manufacturer’s instructions. ABO reverse typing was conducted by agglutination te ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... bacterial genome without bacterial cell lysis. Bactericidal Phage therapy is an application of phages in the treatment of bacterial infections based on the concept the bacteriophages are bacterial specific and bactericidal. The arising bacterial resistance to traditionally used antibacterial and the ...
... bacterial genome without bacterial cell lysis. Bactericidal Phage therapy is an application of phages in the treatment of bacterial infections based on the concept the bacteriophages are bacterial specific and bactericidal. The arising bacterial resistance to traditionally used antibacterial and the ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.