ARTICLES - Weizmann Institute of Science
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
... Eukaryotic genomes are packaged into nucleosome particles that occlude the DNA from interacting with most DNA binding proteins. Nucleosomes have higher affinity for particular DNA sequences, reflecting the ability of the sequence to bend sharply, as required by the nucleosome structure. However, it ...
New Host Plants of Erwinia amylovora in Bulgaria
... were used: one was based on plasmid pEA29 DNA [A (5⬘-CGGTTTTTAACGCTGGG-3⬘) and B (5⬘GGGCAAATACTCGGATT-3⬘) (Bereswill et al., 1992)] and the other was based on genome ams-region [AJ245 (5⬘-AGCTGGCGGGCACTTCACT3⬘) and AJ246 (5⬘-CCCCGCACCGTTCAGTTTT3⬘) (Jones and Geider, 2001)]. The PCR reaction mixture ...
... were used: one was based on plasmid pEA29 DNA [A (5⬘-CGGTTTTTAACGCTGGG-3⬘) and B (5⬘GGGCAAATACTCGGATT-3⬘) (Bereswill et al., 1992)] and the other was based on genome ams-region [AJ245 (5⬘-AGCTGGCGGGCACTTCACT3⬘) and AJ246 (5⬘-CCCCGCACCGTTCAGTTTT3⬘) (Jones and Geider, 2001)]. The PCR reaction mixture ...
A Physical Gene Map of the Bacteriophage P22 Late
... DNA molecules are linear and circularly permuted, the cleavage map is circular (Jackson et al., 1978b). These mature chromosomes are generated by sequential headful packaging from a unique initiation site called pat (Jackson et al., 1978a; Tye et al., 1974). The fragment present in low molar yield i ...
... DNA molecules are linear and circularly permuted, the cleavage map is circular (Jackson et al., 1978b). These mature chromosomes are generated by sequential headful packaging from a unique initiation site called pat (Jackson et al., 1978a; Tye et al., 1974). The fragment present in low molar yield i ...
Processing the RefSeq and CCDS Annotation Datasets Using the SAS System: Creation of Gene Reference
... POINT= option to the SET statement, it is not allowed to be in the output data set. Each time a base is added to the reference gene data set, we increment the value of NN, which is the relative position of the base in the reference gene sequence. Two separated reference gene sequences may contain th ...
... POINT= option to the SET statement, it is not allowed to be in the output data set. Each time a base is added to the reference gene data set, we increment the value of NN, which is the relative position of the base in the reference gene sequence. Two separated reference gene sequences may contain th ...
Maintenance of genomic integrity by p53: complementary
... result, DNA damage will be repaired during a growth arrest, or the damaged cells will be eliminated, thereby preventing ®xation of DNA damage as mutations. This function of p53 led to the now famous coining of p53 as the `guardian of the genome' by Lane (1992). Although the main features of p53's ro ...
... result, DNA damage will be repaired during a growth arrest, or the damaged cells will be eliminated, thereby preventing ®xation of DNA damage as mutations. This function of p53 led to the now famous coining of p53 as the `guardian of the genome' by Lane (1992). Although the main features of p53's ro ...
Weird Alleles, and Intro to Punnett Squares
... will come back a bit next week, and MCAS may choose to use it. So just be aware that these are alleles just like any other, they still stand for different base sequences coding for different versions of a protein, they’re just written by scientists to be more specific. ...
... will come back a bit next week, and MCAS may choose to use it. So just be aware that these are alleles just like any other, they still stand for different base sequences coding for different versions of a protein, they’re just written by scientists to be more specific. ...
Characterisation of Aeromonas species isolated from diseased fish
... further research to test for Minimal Inhibitory Concentrations (MIC) are needed in future to assess the clinical relevance of above mentioned strains. On the basis of biochemical and morphological tests, several of these isolates have been demonstrated to be identical, but genetic studies like PCR-b ...
... further research to test for Minimal Inhibitory Concentrations (MIC) are needed in future to assess the clinical relevance of above mentioned strains. On the basis of biochemical and morphological tests, several of these isolates have been demonstrated to be identical, but genetic studies like PCR-b ...
Directionality in FLP Protein-promoted Site
... efficiency when a mutant site was reacted with an unaltered reaction is detected in this assay only if it occurs between DNA site, reactions involving two identical mutant sites exhibited molecules cut with different restriction enzymes. Reaction between nearly normal levels of recombination (4).Thi ...
... efficiency when a mutant site was reacted with an unaltered reaction is detected in this assay only if it occurs between DNA site, reactions involving two identical mutant sites exhibited molecules cut with different restriction enzymes. Reaction between nearly normal levels of recombination (4).Thi ...
Inhibition of RNA Synthesis by Anthracycline Analogs
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
1 Transmission Disequilibrium Methods for Family
... M 2 / M 2 , then the alleles not transmitted from these parents to the child are used to create a pseudo-control. Since an M 2 allele is not transmitted from each parent, the artificial “genotype” for the pseudo-control is M 2 / M 2 . Then, the frequency of cases that carry the M1 allele can be com ...
... M 2 / M 2 , then the alleles not transmitted from these parents to the child are used to create a pseudo-control. Since an M 2 allele is not transmitted from each parent, the artificial “genotype” for the pseudo-control is M 2 / M 2 . Then, the frequency of cases that carry the M1 allele can be com ...
Comparison of different PCR tests for detecting Shiga toxin
... may arise from more than one strain, hindering interpretation. Ambiguity arises when both stx and rfbO157 genes are detected in a sample containing different strains of E. coli. In this situation, it is not possible to ensure that the signal obtained in multiplex PCR is displayed by STEC O157. The m ...
... may arise from more than one strain, hindering interpretation. Ambiguity arises when both stx and rfbO157 genes are detected in a sample containing different strains of E. coli. In this situation, it is not possible to ensure that the signal obtained in multiplex PCR is displayed by STEC O157. The m ...
... heart. The Kcnq1 promoter strongly associated instead with fragment 2 (Figure 3B, blue line, peak at -12 kb) and showed no contact with fragment 12 in the intron. These data show that there are distinct three-dimensional conformations that regulate expression in each tissue and identify the contact ...
hag expression in Bacillus subtilis is both negatively
... 200 mM of each dNTP. In general, the PCR was performed for 30 cycles with an initial denaturation step at 95 uC for 5 min and a final extension step at 72 uC for 10 min. The temperature and time for the denaturation, annealing and extension steps varied depending on the form of DNA, the Tm of the pr ...
... 200 mM of each dNTP. In general, the PCR was performed for 30 cycles with an initial denaturation step at 95 uC for 5 min and a final extension step at 72 uC for 10 min. The temperature and time for the denaturation, annealing and extension steps varied depending on the form of DNA, the Tm of the pr ...
Distribution and Concordance of N-Acetyltransferase Genotype and
... and therapeutic drugs. Many of the drugs are commonly used and include isoniazid, dapsone, procainamide, and sulfamethazine (5). Carcinogens metabolized by N-acetyltransferase include 2-naphythylamine, 2-aminofluorene, 4-aminobiphenyl, and benzidine (6 – 8). Thus, N-acetylation can modulate drug act ...
... and therapeutic drugs. Many of the drugs are commonly used and include isoniazid, dapsone, procainamide, and sulfamethazine (5). Carcinogens metabolized by N-acetyltransferase include 2-naphythylamine, 2-aminofluorene, 4-aminobiphenyl, and benzidine (6 – 8). Thus, N-acetylation can modulate drug act ...
A homozygous double mutation in SMN1
... available. (b) DNA sequencing results indicate a frameshift in exon 1 due to the 8 bp duplication. The point mutation in exon 5 is shown as a single heterozygous site; normal control is shown for reference. (c) RT-PCR sequencing chromatograms of exons 1, 5, and 7 of SMN1 and SMN2 products in patient ...
... available. (b) DNA sequencing results indicate a frameshift in exon 1 due to the 8 bp duplication. The point mutation in exon 5 is shown as a single heterozygous site; normal control is shown for reference. (c) RT-PCR sequencing chromatograms of exons 1, 5, and 7 of SMN1 and SMN2 products in patient ...
Quantitative Analysis of the Kinetics of End
... All three methods provide reasonably consistent results, but only the last is suf®ciently accurate to determine rate constants. The most convenient method is to monitor declines in the DNA-dependent ATP hydrolysis of RecA protein using the coupled spectrophotometric assay. Although indirect, the met ...
... All three methods provide reasonably consistent results, but only the last is suf®ciently accurate to determine rate constants. The most convenient method is to monitor declines in the DNA-dependent ATP hydrolysis of RecA protein using the coupled spectrophotometric assay. Although indirect, the met ...
New peptide and gene coding for same
... (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisting of synthesized 14 meroligonucleotides labeled with 32P coding for an amino acid sequence of from 12 to 16 of a-hANP (Fig. 9), and 23 clones were selected which hybridized with the probes. Among the 23 c ...
... (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisting of synthesized 14 meroligonucleotides labeled with 32P coding for an amino acid sequence of from 12 to 16 of a-hANP (Fig. 9), and 23 clones were selected which hybridized with the probes. Among the 23 c ...
Taxonomic characterization of Ochrobactrum sp. isolates from soil
... from isolate 1a, SCII24T, OgA9aT, OiC8a, OiC8-6, LMG 5140, CLM14 and CLM18 was amplified by PCR using primers rD1 and fD1 (Weisburg et al., 1991). Amplificates of isolate 1a, SCII24T, OgA9aT, OiC8a and OiC8-6 were purified by low-melting agarose gel electrophoresis and sequenced following the dideox ...
... from isolate 1a, SCII24T, OgA9aT, OiC8a, OiC8-6, LMG 5140, CLM14 and CLM18 was amplified by PCR using primers rD1 and fD1 (Weisburg et al., 1991). Amplificates of isolate 1a, SCII24T, OgA9aT, OiC8a and OiC8-6 were purified by low-melting agarose gel electrophoresis and sequenced following the dideox ...
Instruction Manual for “ChromoPainter: a copying model for
... instance if you condition a subset of haplotypes (or individuals) on every other haplotype (or individual) using the ’-a’ switch, but is useful for tidying the output files. There should be one row in donor list infile for each donor population. There are 2-4 columns per row. The first column gives ...
... instance if you condition a subset of haplotypes (or individuals) on every other haplotype (or individual) using the ’-a’ switch, but is useful for tidying the output files. There should be one row in donor list infile for each donor population. There are 2-4 columns per row. The first column gives ...
Downloaded - Laboratoire de Chimie Théorique
... re-optimized TSs point to the first TS (proton abstraction from O3 0 on the primer base) as rate limiting. This is similar to the exonuclease reaction catalyzed by the e subunit of DNA polymerase III, where once the nucleophile is formed the reaction proceeds downhill.30 The potential energy barriers ...
... re-optimized TSs point to the first TS (proton abstraction from O3 0 on the primer base) as rate limiting. This is similar to the exonuclease reaction catalyzed by the e subunit of DNA polymerase III, where once the nucleophile is formed the reaction proceeds downhill.30 The potential energy barriers ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.