Comprehensive analysis of thiopurine S
... prometheuslabs.com) to individualize treatment with thiopurine drugs. However, the prerequisition for replacing phenotyping by genotyping strategy depends on two requirements: (i) all relevant mutations must be known to explain the different phenotypes and (ii) when genotyping is used in clinical pr ...
... prometheuslabs.com) to individualize treatment with thiopurine drugs. However, the prerequisition for replacing phenotyping by genotyping strategy depends on two requirements: (i) all relevant mutations must be known to explain the different phenotypes and (ii) when genotyping is used in clinical pr ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... compression. Retrieving from compressed data without loss is defined as lossless while recovering from compressed data with data loss is called lossy. Since all the data in a DNA sequence cannot be sacrificed, only lossless compression is applied in DNA compression. The structural information of DNA ...
... compression. Retrieving from compressed data without loss is defined as lossless while recovering from compressed data with data loss is called lossy. Since all the data in a DNA sequence cannot be sacrificed, only lossless compression is applied in DNA compression. The structural information of DNA ...
Molecular insights into mitochondrial transcription and its
... mitochondrial genome (Meisinger et al., 2008). All nuclear encoded factors present in the mitochondrion are translated in the cytoplasm and transported into the mitochondrion by protein import machineries present in the mitochondrial membranes (Figure 2) (Dolezal et al., 2006). Most of these protein ...
... mitochondrial genome (Meisinger et al., 2008). All nuclear encoded factors present in the mitochondrion are translated in the cytoplasm and transported into the mitochondrion by protein import machineries present in the mitochondrial membranes (Figure 2) (Dolezal et al., 2006). Most of these protein ...
MA112 Expresso® Rhamnose SUMO Cloning and
... Vector, the PCR product is simply mixed with the pre-processed Vector and transformed directly into the provided high-efficiency chemically competent cells. Recombination within the host cells seamlessly joins the insert to the vector. Unlike other ligation-independent cloning systems, no enzymatic ...
... Vector, the PCR product is simply mixed with the pre-processed Vector and transformed directly into the provided high-efficiency chemically competent cells. Recombination within the host cells seamlessly joins the insert to the vector. Unlike other ligation-independent cloning systems, no enzymatic ...
Array-based sequencing of filaggrin gene for
... with minimal generation of non-specific products (Figure 1A). Overlapping primer pair assays were designed to provide redundancy in sequencing reads across primer binding sites. In particular, the regions between repeat 7 and repeat 8 or 81 were heavily overlapped with amplicon design to improve cov ...
... with minimal generation of non-specific products (Figure 1A). Overlapping primer pair assays were designed to provide redundancy in sequencing reads across primer binding sites. In particular, the regions between repeat 7 and repeat 8 or 81 were heavily overlapped with amplicon design to improve cov ...
Nucleotide sequence and taxonomic value of the
... 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they contained the same number of characters including padding cha ...
... 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they contained the same number of characters including padding cha ...
Electrophoresis Basi..
... Sample Types Proteins and Electrophoresis Isozymes (isoenzymes): enzymes that differ in amino acid sequence but catalyze the same chemical reaction. Isozymes have different molecular weights and chemical properties and thus can be separated from each other during electrophoresis. Isozymes are coded ...
... Sample Types Proteins and Electrophoresis Isozymes (isoenzymes): enzymes that differ in amino acid sequence but catalyze the same chemical reaction. Isozymes have different molecular weights and chemical properties and thus can be separated from each other during electrophoresis. Isozymes are coded ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... There is also a global property called secondary structure. For ds-DNA, for instance, there exist several different types of helices. The most common ones are called A-DNA and B-DNA. What changes the global structure of DNA between A- and B-DNA is the ionic and water environment. B-DNA is preferred ...
... There is also a global property called secondary structure. For ds-DNA, for instance, there exist several different types of helices. The most common ones are called A-DNA and B-DNA. What changes the global structure of DNA between A- and B-DNA is the ionic and water environment. B-DNA is preferred ...
SCI 30 UA CH 2.2 Inheritance
... Although people have long understood that characteristics are inherited from their parents, they did not understand the mechanisms that enable inheritance to happen. As Mendel’s work became more well known and understood, scientists were able to use his observations, terminology, and the results o ...
... Although people have long understood that characteristics are inherited from their parents, they did not understand the mechanisms that enable inheritance to happen. As Mendel’s work became more well known and understood, scientists were able to use his observations, terminology, and the results o ...
Can my homozygous polled bull give me scurred calves?
... from the cow to make its pair. If a calf gets a polled allele from either parent then it will be polled. If it gets two polled alleles it is considered homozygous polled; if it gets one polled and one horn allele it will be physically polled, but it will be referred to as heterozygous polled or ...
... from the cow to make its pair. If a calf gets a polled allele from either parent then it will be polled. If it gets two polled alleles it is considered homozygous polled; if it gets one polled and one horn allele it will be physically polled, but it will be referred to as heterozygous polled or ...
Supplementary Materials and methods (doc 154K)
... strains carrying the different plasmids during flow cytometry, and controlled using confocal microscopy. One possible factor that would have altered the result of the competitions would be plasmid transfer by conjugation from PAO1 to PAO1-GFP. If plasmids were transferred to PAO1-GFP they would prod ...
... strains carrying the different plasmids during flow cytometry, and controlled using confocal microscopy. One possible factor that would have altered the result of the competitions would be plasmid transfer by conjugation from PAO1 to PAO1-GFP. If plasmids were transferred to PAO1-GFP they would prod ...
Application Note: Targeted sequencing and chromosomal haplotype
... Single Molecule, Real-Time (SMRT®) Sequencing provides high consensus accuracy and long read lengths. As such, it enables end-to-end sequencing of multi-kilobase TLA amplicons or unamplified TLA templates. The combination of Cergentis’ TLA and SMRT Sequencing technologies allows for sequencing and h ...
... Single Molecule, Real-Time (SMRT®) Sequencing provides high consensus accuracy and long read lengths. As such, it enables end-to-end sequencing of multi-kilobase TLA amplicons or unamplified TLA templates. The combination of Cergentis’ TLA and SMRT Sequencing technologies allows for sequencing and h ...
Developing expressed sequence tag libraries and
... 3 bp repeats, are more common repeat lengths for gene coding regions, likely because their increase or decrease in repeat number does not cause a reading frame shift [37]. This dataset did demonstrate this tendency with 30 % dimers (2 bp repeat motif ), 44 % trimers (3 bp repeat motif ), 20 % tetram ...
... 3 bp repeats, are more common repeat lengths for gene coding regions, likely because their increase or decrease in repeat number does not cause a reading frame shift [37]. This dataset did demonstrate this tendency with 30 % dimers (2 bp repeat motif ), 44 % trimers (3 bp repeat motif ), 20 % tetram ...
Investigating the DNA-Binding Interactions of Small
... and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a highly sensitive methodology to determine the DNA-binding mode of small molecules unambiguously unlike other conventional techniques. Furthermore, we established struc ...
... and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a highly sensitive methodology to determine the DNA-binding mode of small molecules unambiguously unlike other conventional techniques. Furthermore, we established struc ...
Stage-specific expressions of four different ribonuclease H genes in
... protocols (see below). Full-length cDNA for each gene was generated by recombination PCR (Sambrook and Russell 2001) using the 5′- and 3′-RACE products as templates and the miniexon-specific primer and AUAP as primers (see below). Following terminal primers were used to amplify the coding sequence o ...
... protocols (see below). Full-length cDNA for each gene was generated by recombination PCR (Sambrook and Russell 2001) using the 5′- and 3′-RACE products as templates and the miniexon-specific primer and AUAP as primers (see below). Following terminal primers were used to amplify the coding sequence o ...
Electrophoretic karyotypes of clinically isolated yeasts
... (PFGE) (Kaufmann & Merz, 1989;Mahrous et al., 1990; Merz et al., 1988; Monod et al., 1990). These polymorphisms have made it possible to distinguish strains efficiently. C. albicans is one of the organisms in which karyotypes analysed by PFGE have been most studied (Scherer & Abbreviation: PFGE, pul ...
... (PFGE) (Kaufmann & Merz, 1989;Mahrous et al., 1990; Merz et al., 1988; Monod et al., 1990). These polymorphisms have made it possible to distinguish strains efficiently. C. albicans is one of the organisms in which karyotypes analysed by PFGE have been most studied (Scherer & Abbreviation: PFGE, pul ...
Application of whole genome sequencing to fully characterise
... Campylobacter is the most common cause of acute bacterial gastroenteritis worldwide. In the UK alone it causes an estimated 500,000 infections each year. There have been two large studies of Infectious Intestinal Disease in the UK community (IID1 in the mid 1990s and IID2 in 2008-2009). In both stud ...
... Campylobacter is the most common cause of acute bacterial gastroenteritis worldwide. In the UK alone it causes an estimated 500,000 infections each year. There have been two large studies of Infectious Intestinal Disease in the UK community (IID1 in the mid 1990s and IID2 in 2008-2009). In both stud ...
HLA-A, -B
... A*0101, *0301, Cw*0701,*0702, B*0702,*0801, DRB1*0301,*1501, DQA1*0501,*0102, DQB1*0201,*0602 composed from 2 haplotypes from the parents: A*0101 : Cw*0701 : B*0801 : DRB1*0301 : DQA1*0501 : DQB1*0201 (by serotyping A1-Cw7-B8-DR3-DQ2) A*0301 : Cw*0702 : B*0702 : DRB1*1501 : DQA1*0102 : DQB1*0602 (by ...
... A*0101, *0301, Cw*0701,*0702, B*0702,*0801, DRB1*0301,*1501, DQA1*0501,*0102, DQB1*0201,*0602 composed from 2 haplotypes from the parents: A*0101 : Cw*0701 : B*0801 : DRB1*0301 : DQA1*0501 : DQB1*0201 (by serotyping A1-Cw7-B8-DR3-DQ2) A*0301 : Cw*0702 : B*0702 : DRB1*1501 : DQA1*0102 : DQB1*0602 (by ...
Procedure - Peevyhouse
... actually occurs in the frequency of gene alleles in the population. William Castle, an American scientist; Geoffrey Hardy, a British mathematician; and Wilhelm Weinberg, a German physician, independently determined that the frequencies of genes in a population remain constant unless certain forces a ...
... actually occurs in the frequency of gene alleles in the population. William Castle, an American scientist; Geoffrey Hardy, a British mathematician; and Wilhelm Weinberg, a German physician, independently determined that the frequencies of genes in a population remain constant unless certain forces a ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.