Role of base-backbone and base-base interactions
... helical twist than the average (Table 1). In contrast, the YR steps in B-DNA and in the protein complexes are characterised with high standard deviations in roll, helical twist, and slide parameters, and thus these are more flexible than the others. The slide parameter at YR steps is positive on the ...
... helical twist than the average (Table 1). In contrast, the YR steps in B-DNA and in the protein complexes are characterised with high standard deviations in roll, helical twist, and slide parameters, and thus these are more flexible than the others. The slide parameter at YR steps is positive on the ...
Three-dimensional Structures of Bulge
... frame. This mutation, termed a frameshift, generally produces a protein with an incorrect sequence following the site of mutation, usually resulting in a non-functioning gene product. Frameshifts that result from unpaired nucleotides can arise from recombination processes or from displacement of bas ...
... frame. This mutation, termed a frameshift, generally produces a protein with an incorrect sequence following the site of mutation, usually resulting in a non-functioning gene product. Frameshifts that result from unpaired nucleotides can arise from recombination processes or from displacement of bas ...
Smoking, Genes, and Health - The Center for Experimental Social
... polymorphisms, or SNPs) in nicotinic receptor genes that impact measured smoking behavior.1 While the genetics literature has produced a set of credible gene-smoking associations, little is known about how the biological processes affected by these genes map into the behavioral mechanisms that deter ...
... polymorphisms, or SNPs) in nicotinic receptor genes that impact measured smoking behavior.1 While the genetics literature has produced a set of credible gene-smoking associations, little is known about how the biological processes affected by these genes map into the behavioral mechanisms that deter ...
how to solve genetics problems
... from their mother. Since we know the mother has a C allele, the other one must be c. Therefore, the mother’s genotype is Ccbb. The checkered brown pigeon got one b allele from his father and the other b allele from his mother. Since we know his father has one B allele, the other one must be b. There ...
... from their mother. Since we know the mother has a C allele, the other one must be c. Therefore, the mother’s genotype is Ccbb. The checkered brown pigeon got one b allele from his father and the other b allele from his mother. Since we know his father has one B allele, the other one must be b. There ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
... TEUF0537, and TEUF0056 hybridized only with band XVII (Table 1). These results could be explained by the presence of nonhomologous chromosomes in both bands XVI and XVII. To test this hypothesis, the chromosomal bands XVI and XVII were digested with NotI and hybridized with a T. cruzi telomeric sequ ...
... TEUF0537, and TEUF0056 hybridized only with band XVII (Table 1). These results could be explained by the presence of nonhomologous chromosomes in both bands XVI and XVII. To test this hypothesis, the chromosomal bands XVI and XVII were digested with NotI and hybridized with a T. cruzi telomeric sequ ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... protect a plasmid repair intermediate that would otherwise be destroyed by cellular nucleases. An inverted dimer plasmid could be formed from one plasmid molecule that is replicated after its entry into thecell. Such a mechanism might account for the head-to-head union of the daughter DNA duplexes. ...
... protect a plasmid repair intermediate that would otherwise be destroyed by cellular nucleases. An inverted dimer plasmid could be formed from one plasmid molecule that is replicated after its entry into thecell. Such a mechanism might account for the head-to-head union of the daughter DNA duplexes. ...
Echinomycin binding to alternating AT
... surrounding blocks of alternating AT, dramatically increasing the cleavage of some, but not all, the intervening TpA steps. These results are qualitatively similar to those reported in the preceding paper for T(AT)8CG(AT),5 [10]. This can be seen more clearly in the cleavage histograms presented in ...
... surrounding blocks of alternating AT, dramatically increasing the cleavage of some, but not all, the intervening TpA steps. These results are qualitatively similar to those reported in the preceding paper for T(AT)8CG(AT),5 [10]. This can be seen more clearly in the cleavage histograms presented in ...
Computer Simulation using AlleleA1
... recessive state by changing the fitness of the A1A1 genotype to 0.9. Notice that allele A1, although it is harmful, is not eliminated from this population but instead is maintained at a very low frequency. (Note: You can confirm this by running this simulation over 100,000 generations). There are tw ...
... recessive state by changing the fitness of the A1A1 genotype to 0.9. Notice that allele A1, although it is harmful, is not eliminated from this population but instead is maintained at a very low frequency. (Note: You can confirm this by running this simulation over 100,000 generations). There are tw ...
Molecular Characterization of a Powdery Mildew Resistance Gene
... Wheat powdery mildew, caused by the obligate biotrophic parasitic fungus Blumeria graminis (DC) E.O. Speer f. sp. tritici, is an important foliar disease of wheat worldwide and is promulgated by the heavy use of high-yielding semidwarf cultivars, irrigation, and nitrogen fertilizer (1). Resistance t ...
... Wheat powdery mildew, caused by the obligate biotrophic parasitic fungus Blumeria graminis (DC) E.O. Speer f. sp. tritici, is an important foliar disease of wheat worldwide and is promulgated by the heavy use of high-yielding semidwarf cultivars, irrigation, and nitrogen fertilizer (1). Resistance t ...
Deoxyribonucleic Acid Base Compositions and Nucleotide
... guanine-plus-cytosine (G+C) frequency curves (6), describe an additional physicochemical quality of the bacterial genome. The DNA nucleotide distributions of some 2,500 bacterial strains were found to be “quite similar within each genus,” but varied considerably between genera (6), thus providing us ...
... guanine-plus-cytosine (G+C) frequency curves (6), describe an additional physicochemical quality of the bacterial genome. The DNA nucleotide distributions of some 2,500 bacterial strains were found to be “quite similar within each genus,” but varied considerably between genera (6), thus providing us ...
the association of chloroplast dna with photosynthetic membrane
... Further autoradiographs of chloroplast photosynthetic membranes are shown in Figs. 16 and 17 from a separate experiment. The underlying membranes can be seen more readily in these photographs. Fig. 16 is the type of pattern shown in Figs. 1015 where there are peripheral grana rich regions. Fig. 17 s ...
... Further autoradiographs of chloroplast photosynthetic membranes are shown in Figs. 16 and 17 from a separate experiment. The underlying membranes can be seen more readily in these photographs. Fig. 16 is the type of pattern shown in Figs. 1015 where there are peripheral grana rich regions. Fig. 17 s ...
DNA Three Way Junction Core Decorated with Amino Acids
... in the active site [1,2]. Therefore, it has been tempting to search for nucleic acid catalysts that could perform the reverse reaction, i.e., peptide bond hydrolysis that is commonly assumed by protease under mild conditions. Most of the efforts towards DNA-based artificial protease have been conduc ...
... in the active site [1,2]. Therefore, it has been tempting to search for nucleic acid catalysts that could perform the reverse reaction, i.e., peptide bond hydrolysis that is commonly assumed by protease under mild conditions. Most of the efforts towards DNA-based artificial protease have been conduc ...
HLA matching, compatibility testing and donor selection
... Medium resolution typing implies that a DNA based test has been used, but it not discriminatory enough to tell us the exact allele i.e. it can define specific allele groups (often as a ‘string’ of possible alleles). For example an HLA-A*0201 allele may be typed as an A*0201/0205/0209/0240 with the t ...
... Medium resolution typing implies that a DNA based test has been used, but it not discriminatory enough to tell us the exact allele i.e. it can define specific allele groups (often as a ‘string’ of possible alleles). For example an HLA-A*0201 allele may be typed as an A*0201/0205/0209/0240 with the t ...
Non-invasive prenatal assessment of trisomy 21 by multiplexed
... markers in maternal plasma, has been proposed for non-invasive detection of fetal aneuploidies.15-19 The advantages and disadvantages of these approaches have been reviewed in detail.20 An alternative approach for non-invasive prenatal diagnosis of fetal trisomy 21 is to show the presence of an elev ...
... markers in maternal plasma, has been proposed for non-invasive detection of fetal aneuploidies.15-19 The advantages and disadvantages of these approaches have been reviewed in detail.20 An alternative approach for non-invasive prenatal diagnosis of fetal trisomy 21 is to show the presence of an elev ...
Analysis and nucleotide sequence of an origin of DNA replication in
... Fig.2. Physical maps of deletion derivatives from pWHI266. The A. calcoaceticus.derived portion of pWH1266 (see Fig. !) with some restriction sites is displayed on the top. The pBR322 derived sequences of the deleted plasmids are omitted, pWH1269, 1270, 1273 and 1274 contain the pBR322 portion in op ...
... Fig.2. Physical maps of deletion derivatives from pWHI266. The A. calcoaceticus.derived portion of pWH1266 (see Fig. !) with some restriction sites is displayed on the top. The pBR322 derived sequences of the deleted plasmids are omitted, pWH1269, 1270, 1273 and 1274 contain the pBR322 portion in op ...
DNA methylation controls histone H3 lysine 9 methylation
... 2001; Lachner and Jenuwein, 2002; Richards and Elgin, 2002). The interactions between DNA methylation, histone modi®cations and chromatin structure have mainly been studied at the molecular level for speci®c DNA sequences. Integrated genetic, molecular and cytological approaches can provide new insi ...
... 2001; Lachner and Jenuwein, 2002; Richards and Elgin, 2002). The interactions between DNA methylation, histone modi®cations and chromatin structure have mainly been studied at the molecular level for speci®c DNA sequences. Integrated genetic, molecular and cytological approaches can provide new insi ...
Phytopathology
... is an emerging methodology useful for identification of DNA fragments and may be applicable for rapid identification and detection of plant pathogens associated with plants (20,21,26). An array of species-specific oligonucleotide probes representing the various pathogens of potato, built on a solid ...
... is an emerging methodology useful for identification of DNA fragments and may be applicable for rapid identification and detection of plant pathogens associated with plants (20,21,26). An array of species-specific oligonucleotide probes representing the various pathogens of potato, built on a solid ...
Assaying … promoter activity
... nimB and ORF5468 gene. We tested the stability of the integrated plasmid via a true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic selection for approximately 10 generations. Following this period, aliquots from th ...
... nimB and ORF5468 gene. We tested the stability of the integrated plasmid via a true breeding experiment. In this experiment, recombinant cells were grown at the non-permissive temperature in the absence of antibiotic selection for approximately 10 generations. Following this period, aliquots from th ...
Misidentification of a genomovar of Burkholderia cepacia by recA
... Overall, it is important to recognise the consequences for the patient of the misinterpretation of RFLP profiles, for a combination of reasons, as stated above. Minor banding differences can result in changes from Genomovar V to IIIb status, as was illustrated in this case report, with important imp ...
... Overall, it is important to recognise the consequences for the patient of the misinterpretation of RFLP profiles, for a combination of reasons, as stated above. Minor banding differences can result in changes from Genomovar V to IIIb status, as was illustrated in this case report, with important imp ...
Efficient Family Based Association Tests
... Model for Association. For each of the genotyped SNP markers, we are interested in testing whether observed genotypes and phenotypes are associated. For the SNP being tested, we label the two alleles ‘A’ and ‘a’ and define a genotype score gijm as 0, 1 or 2 depending on whether Gijm = a/a, A/a or A/ ...
... Model for Association. For each of the genotyped SNP markers, we are interested in testing whether observed genotypes and phenotypes are associated. For the SNP being tested, we label the two alleles ‘A’ and ‘a’ and define a genotype score gijm as 0, 1 or 2 depending on whether Gijm = a/a, A/a or A/ ...
Molecular genotyping of ABO blood groups in some population
... glycosyltransferase sequence is considered as a reference sequence and compared to it B and O groups are identified. Initially specific restriction endonucleases were used to identify common ABO alleles2. Later on by using gene scanning techniques like denaturing gradient gel electrophoresis (DGGE ...
... glycosyltransferase sequence is considered as a reference sequence and compared to it B and O groups are identified. Initially specific restriction endonucleases were used to identify common ABO alleles2. Later on by using gene scanning techniques like denaturing gradient gel electrophoresis (DGGE ...
Accepted Version - CSIRO Research Publications Repository
... Variation in DNA sequence can cause variation in gene expression, which influences quantitative phenotypic variation in organisms and is an important factor in natural variation. Gene expression regulatory networks are comprised of cis- and trans-acting factors, and differences in gene expression ar ...
... Variation in DNA sequence can cause variation in gene expression, which influences quantitative phenotypic variation in organisms and is an important factor in natural variation. Gene expression regulatory networks are comprised of cis- and trans-acting factors, and differences in gene expression ar ...
Part 4 Student Handout - URMC
... Testing for the HD Gene Narrator: Jenny, Jeremy, and their father have decided to undergo genetic testing for Huntington’s disease. Today, our lab groups will act as gene testing laboratory technicians. We will use the gel electrophoresis laboratory procedure to analyze the results of the simulated ...
... Testing for the HD Gene Narrator: Jenny, Jeremy, and their father have decided to undergo genetic testing for Huntington’s disease. Today, our lab groups will act as gene testing laboratory technicians. We will use the gel electrophoresis laboratory procedure to analyze the results of the simulated ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.