Biophysics 101 Genomics and Computational Biology
... Selection and characterization of amino acid substitutions at residues 237-240 of TEM-1 beta-lactamase with altered substrate specificity Selection strategy for site-directed mutagenesis based on altered beta-lactamase specificity. Site-directed mutagenesis of yeast eEF1A. Viable mutants with altere ...
... Selection and characterization of amino acid substitutions at residues 237-240 of TEM-1 beta-lactamase with altered substrate specificity Selection strategy for site-directed mutagenesis based on altered beta-lactamase specificity. Site-directed mutagenesis of yeast eEF1A. Viable mutants with altere ...
Short Tandem Repeat Polymorphism and Cancer Risk: Influence of
... repeats and prostate cancer risk, whereas two thirds did not find the association. The inconsistency has been attributed to the differences in study populations, which include patient age as well as the time and method of disease detection. Whereas these variables are possible explanations for the i ...
... repeats and prostate cancer risk, whereas two thirds did not find the association. The inconsistency has been attributed to the differences in study populations, which include patient age as well as the time and method of disease detection. Whereas these variables are possible explanations for the i ...
"big IB objectives"-use the blank paper technique
... 3.4.1 – explain DNA replication in terms of unwinding the double helix and separation of the strands by helicase, followed by formation of the new complementary strands by DNA polymerase 3.4.2 – explain the significance of complementary base pairing in the conservation of the base sequence of DNA 3 ...
... 3.4.1 – explain DNA replication in terms of unwinding the double helix and separation of the strands by helicase, followed by formation of the new complementary strands by DNA polymerase 3.4.2 – explain the significance of complementary base pairing in the conservation of the base sequence of DNA 3 ...
Isolation, Identification, and Enumeration of Pathogenic Salmonella
... Multiplex PCR Kit (Qiagen, Hilden, Germany) along with the appropriate primer set, all of which is contained in a sterilized 0.5 mL PCR tube (modified from Hassanein et al. 2011). PCR kits typically contain Taq polymerase (an enzyme that anneals nucleotides to DNA strands; capable of surviving the h ...
... Multiplex PCR Kit (Qiagen, Hilden, Germany) along with the appropriate primer set, all of which is contained in a sterilized 0.5 mL PCR tube (modified from Hassanein et al. 2011). PCR kits typically contain Taq polymerase (an enzyme that anneals nucleotides to DNA strands; capable of surviving the h ...
Hd6, a rice quantitative trait locus involved in photoperiod
... series of genes and show continuous variation in progeny. Many efforts have been made to map such genes, known as quantitative trait loci (QTLs), because of their biological and agronomic importance. The development of a high-density linkage map based on DNA markers made it possible to map QTLs with ...
... series of genes and show continuous variation in progeny. Many efforts have been made to map such genes, known as quantitative trait loci (QTLs), because of their biological and agronomic importance. The development of a high-density linkage map based on DNA markers made it possible to map QTLs with ...
Abbott RealTime HIV-1
... A total of 88 clinical specimens, ten of each Group M subtype (A, B, C, D, CRF01-AE, F, CRF02-AG, G) and eight of Group O, were tested with the RealTime HIV-1 assay and by two other HIV-1 quantitative assays referred to as Comparator 1 and Comparator 2. The numbers in parentheses are the number of s ...
... A total of 88 clinical specimens, ten of each Group M subtype (A, B, C, D, CRF01-AE, F, CRF02-AG, G) and eight of Group O, were tested with the RealTime HIV-1 assay and by two other HIV-1 quantitative assays referred to as Comparator 1 and Comparator 2. The numbers in parentheses are the number of s ...
Glycolysis Cell-Based Assay Kit
... The vial contains a lyophilized powder of enzymes. Immediately prior to use, reconstitute the contents of the vial with 150 µl of Assay Buffer. If you are not using all of the mixture at one time, make small aliquots and store at -80°C. Freezing and thawing of this solution should be limited to a si ...
... The vial contains a lyophilized powder of enzymes. Immediately prior to use, reconstitute the contents of the vial with 150 µl of Assay Buffer. If you are not using all of the mixture at one time, make small aliquots and store at -80°C. Freezing and thawing of this solution should be limited to a si ...
Towards Programmable Molecular Machines
... a molecule of the form ABC into AB and BC but can only make the cut at a fixed location with respect to the restriction site. We also assume that the restriction site can be two regions separated by a fixed distance, since this type of enzyme exists in nature and is used in previous constructions [1 ...
... a molecule of the form ABC into AB and BC but can only make the cut at a fixed location with respect to the restriction site. We also assume that the restriction site can be two regions separated by a fixed distance, since this type of enzyme exists in nature and is used in previous constructions [1 ...
Finishing the Human Genome
... Doug Brutlag Professor Emeritus of Biochemistry & Medicine Stanford University School of Medicine Doug Brutlag 2011 ...
... Doug Brutlag Professor Emeritus of Biochemistry & Medicine Stanford University School of Medicine Doug Brutlag 2011 ...
Thermodynamic analysis of DNA binding by a Bacillus single
... Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high affinity comparable to other prokaryotic SSBs. Thermodynamic analysis indicated both hydrophobic and ionic contributions to ssDNA binding. FRET analysis of oligo(dT)70 binding suggested that SSBBA forms a tetrameric assemb ...
... Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high affinity comparable to other prokaryotic SSBs. Thermodynamic analysis indicated both hydrophobic and ionic contributions to ssDNA binding. FRET analysis of oligo(dT)70 binding suggested that SSBBA forms a tetrameric assemb ...
Cloning and Molecular Analysis of the Plasma ... Paramecium tetraurelia
... chain reaction (PCR) and sequencing (except the T,and T,primers from Stratagene, San Diego, CA) were made in an Applied Biosystems DNA (Foster City, CA) synthesizer model 391. Cloning of polymerase chain reaction products. The first sets of primers were designed to amplify an internal segment of P t ...
... chain reaction (PCR) and sequencing (except the T,and T,primers from Stratagene, San Diego, CA) were made in an Applied Biosystems DNA (Foster City, CA) synthesizer model 391. Cloning of polymerase chain reaction products. The first sets of primers were designed to amplify an internal segment of P t ...
Molecular Analysis of the Coprinus cinereus Mating Type A Factor
... DAY(1 960, 1963b) that the least two closely linked subunits, termed (Y and B. T h e functions of(Y and B appear redundantbecause genetic analysis has shown that an allelic difference at only a single subunit is sufficient for compatibility at A. Because the subunits themselves have many allelic for ...
... DAY(1 960, 1963b) that the least two closely linked subunits, termed (Y and B. T h e functions of(Y and B appear redundantbecause genetic analysis has shown that an allelic difference at only a single subunit is sufficient for compatibility at A. Because the subunits themselves have many allelic for ...
Equilibrium Statistics of Channel-confined DNA
... Introduction Chapter 1. Introduction This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels wi ...
... Introduction Chapter 1. Introduction This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels wi ...
The Large Loop Repair and Mismatch Repair Pathways
... mitotic growth. NER functions to repair bulky DNA lesions, such as thymine dimers and other helix-distorting lesions. During NER the damaged nucleotide is recognized and bound by several NER proteins, and the DNA surrounding the lesion is unwound. The single-stranded DNA containing the lesion is rem ...
... mitotic growth. NER functions to repair bulky DNA lesions, such as thymine dimers and other helix-distorting lesions. During NER the damaged nucleotide is recognized and bound by several NER proteins, and the DNA surrounding the lesion is unwound. The single-stranded DNA containing the lesion is rem ...
PDF - Molecular Cytogenetics
... spermatogenesis, characterized by azoospermia or severe oligozoospermia [7]. The causes of this type of spermatogenesis failure are not fully understood. However, spermatogenesis is generally much more likely to involve meiotic disruption, when compared with oogenesis, due to the more efficient meio ...
... spermatogenesis, characterized by azoospermia or severe oligozoospermia [7]. The causes of this type of spermatogenesis failure are not fully understood. However, spermatogenesis is generally much more likely to involve meiotic disruption, when compared with oogenesis, due to the more efficient meio ...
a nine-base pair deletion distinguishes two en/spm
... The En insertions in the distinguishable a1-m(papu) and a1-m(Au) alleles reside at the same site in exon 2 of the A1 gene. Their phenotypic difference (Figures 1 and 2) can be ascribed to the nine-base pair deletion in the distal side (3') of the insert, 45 bases away from the site of the insert. Ea ...
... The En insertions in the distinguishable a1-m(papu) and a1-m(Au) alleles reside at the same site in exon 2 of the A1 gene. Their phenotypic difference (Figures 1 and 2) can be ascribed to the nine-base pair deletion in the distal side (3') of the insert, 45 bases away from the site of the insert. Ea ...
Identification of loci affecting teat number by
... and F2) were used to track the QTL genotype in the F2 animals; while the SNP genotypes were considered as causative SNP genotypes in GWAS. Although we detected few significant loci in the GWAS, we obtained important clues for the most likely locations of these loci by using high-density SNPs across ...
... and F2) were used to track the QTL genotype in the F2 animals; while the SNP genotypes were considered as causative SNP genotypes in GWAS. Although we detected few significant loci in the GWAS, we obtained important clues for the most likely locations of these loci by using high-density SNPs across ...
Aminoacyl-tRNA Synthetase Production for Unnatural Amino Acid
... technique, rather than using living cells to make protein, simply extracts the cells’ natural protein-making machinery and then uses it to produce protein in vitro. Because living cells are no longer involved, scientists can freely adapt the protein production environment in ways not otherwise possi ...
... technique, rather than using living cells to make protein, simply extracts the cells’ natural protein-making machinery and then uses it to produce protein in vitro. Because living cells are no longer involved, scientists can freely adapt the protein production environment in ways not otherwise possi ...
The rapidly evolving field of plant centromeres
... differ in their capacity to promote chromosome segregation [11], and several trans-acting factors that affect knob activation have been identified [39]. At least four distinct genetic functions mapping to Abnormal chromosome 10 (Ab10) play a role in the segregation of knobs; in some cases, these hav ...
... differ in their capacity to promote chromosome segregation [11], and several trans-acting factors that affect knob activation have been identified [39]. At least four distinct genetic functions mapping to Abnormal chromosome 10 (Ab10) play a role in the segregation of knobs; in some cases, these hav ...
Complete Thyroxine-Binding Globulin (TBG) Deficiency Produced
... Two consecutive PCRs were needed to amplify a TBG cDNA fragment across exon 2, Oligonucleotide primers for the first PCR were: 59-GCATCTGATCTGTTCACTGAATTTC-39 sense, nucleotide (nt) 339 – 364 and 59-GTTTCAGACCATTGTCCTCT-39 antisense, nt 2798 –2817. A second, nested PCR, used the following oligonucle ...
... Two consecutive PCRs were needed to amplify a TBG cDNA fragment across exon 2, Oligonucleotide primers for the first PCR were: 59-GCATCTGATCTGTTCACTGAATTTC-39 sense, nucleotide (nt) 339 – 364 and 59-GTTTCAGACCATTGTCCTCT-39 antisense, nt 2798 –2817. A second, nested PCR, used the following oligonucle ...
Nucleosides, Nucleotides,Nucleic Acids
... mechanism of DNA replication. Transcription begins at the 5' end of DNA and is catalyzed by the enzyme RNA polymerase. Dr. Wolf's CHM 424 ...
... mechanism of DNA replication. Transcription begins at the 5' end of DNA and is catalyzed by the enzyme RNA polymerase. Dr. Wolf's CHM 424 ...
Introduction - bei DuEPublico
... concept. In general this concept assumes that a cell that is defective in only one of its two alleles behaves as a normal cell, i.e. there is no loss of control until both gene copies are put out of action. However, loss-of-function of one allele may enhance the susceptibility to cancer. The first a ...
... concept. In general this concept assumes that a cell that is defective in only one of its two alleles behaves as a normal cell, i.e. there is no loss of control until both gene copies are put out of action. However, loss-of-function of one allele may enhance the susceptibility to cancer. The first a ...
NanoString™: User Guide | nCounter® Expression Data Analysis
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.