Document
... monitored from the PCR reactions preparation step to bioinformatics analysis avoiding sample-crossing throughout the protocol. ...
... monitored from the PCR reactions preparation step to bioinformatics analysis avoiding sample-crossing throughout the protocol. ...
Nucleotide Metabolism
... Nucleotide Metabolism Proceeds Through de novo and Salvage Pathways Purine Nucleotides are Built de novo Starting with Ribose-5-phosphate PRPP is Made From it and Then it is Aminated Simple Compounds, Such as Amino Acids and 1-Carbon Donors Make the Bases IMP is a Branch Point for Synthesis of GMP a ...
... Nucleotide Metabolism Proceeds Through de novo and Salvage Pathways Purine Nucleotides are Built de novo Starting with Ribose-5-phosphate PRPP is Made From it and Then it is Aminated Simple Compounds, Such as Amino Acids and 1-Carbon Donors Make the Bases IMP is a Branch Point for Synthesis of GMP a ...
Ribotyping of Clostridium perfringens from industrially produced
... the reproducibility of a typing method, which is the percentage of strains with the same subtype on repeated testing, preferably after a period of a few months. Second, the discriminatory power of a method is an estimate of its ability to differentiate between two unrelated strains (Hunter 1990; Swa ...
... the reproducibility of a typing method, which is the percentage of strains with the same subtype on repeated testing, preferably after a period of a few months. Second, the discriminatory power of a method is an estimate of its ability to differentiate between two unrelated strains (Hunter 1990; Swa ...
The effecT of chlorinaTion of nucleoTide bases on The
... Recent studies on Escherichia coli bacteria cultivation, in which DNA thymine was replaced with 5-chlorouracil have refreshed the problem of understanding the changes to physical properties of DNA monomers resultant from chemical modifications. These studies have shown that the replacement did not a ...
... Recent studies on Escherichia coli bacteria cultivation, in which DNA thymine was replaced with 5-chlorouracil have refreshed the problem of understanding the changes to physical properties of DNA monomers resultant from chemical modifications. These studies have shown that the replacement did not a ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... Phage yields were computed without correction for losses from titrations of the low speed supernatants. These were frequently checked against titers obtained by the cyanide dilution method (Doermann, 1952), which usually ran 10 to 30 per cent higher. Finally, both low and high speed sediments were s ...
... Phage yields were computed without correction for losses from titrations of the low speed supernatants. These were frequently checked against titers obtained by the cyanide dilution method (Doermann, 1952), which usually ran 10 to 30 per cent higher. Finally, both low and high speed sediments were s ...
Plant Functional Genomics Plant Functional Genomics
... 1. Set up 4–6 restriction digestions, each digesting 5 µg pCUGIBAC1 plasmid DNA (with HindIII, EcoRI, or BamHI depending on which enzyme is selected for BAC library construction) in 150 µL 1× TA buffer at 37°C for 2 h. Check 1 µL on a 1% agarose minigel to determine if the plasmid is digested. 2. He ...
... 1. Set up 4–6 restriction digestions, each digesting 5 µg pCUGIBAC1 plasmid DNA (with HindIII, EcoRI, or BamHI depending on which enzyme is selected for BAC library construction) in 150 µL 1× TA buffer at 37°C for 2 h. Check 1 µL on a 1% agarose minigel to determine if the plasmid is digested. 2. He ...
Epigenetic Interactions among Three dTph1
... (A) Sequence analysis of PCR-amplified excision products generated by the transposon in class 1 an3 alleles. For each an3 allele, the position of the insertion is indicated by an arrow, and the direction is as given in Figure 1. The target site duplications flanking the transposon are underlined. Ex ...
... (A) Sequence analysis of PCR-amplified excision products generated by the transposon in class 1 an3 alleles. For each an3 allele, the position of the insertion is indicated by an arrow, and the direction is as given in Figure 1. The target site duplications flanking the transposon are underlined. Ex ...
WOTD - Brookwood High School
... V: incomplete dominance neither allele is dominant to the other, both are expressed equally, often a blending of the two traits occurs ...
... V: incomplete dominance neither allele is dominant to the other, both are expressed equally, often a blending of the two traits occurs ...
Epigenetic Interactions among Three dTph1 Transposons in Two
... (A) Sequence analysis of PCR-amplified excision products generated by the transposon in class 1 an3 alleles. For each an3 allele, the position of the insertion is indicated by an arrow, and the direction is as given in Figure 1. The target site duplications flanking the transposon are underlined. Ex ...
... (A) Sequence analysis of PCR-amplified excision products generated by the transposon in class 1 an3 alleles. For each an3 allele, the position of the insertion is indicated by an arrow, and the direction is as given in Figure 1. The target site duplications flanking the transposon are underlined. Ex ...
Novel pathogen-specific primers for the detection of Agrobacterium
... crown gall (BURR et al. 1998). Besides A. vitis, Agrobacterium tumefaciens may also occur on grapevines as causative agent of crown gall disease (SZEGEDI et al. 2005). The polymerase chain reaction (PCR) has widely been used to test for the presence of various plant pathogens to select clean plant m ...
... crown gall (BURR et al. 1998). Besides A. vitis, Agrobacterium tumefaciens may also occur on grapevines as causative agent of crown gall disease (SZEGEDI et al. 2005). The polymerase chain reaction (PCR) has widely been used to test for the presence of various plant pathogens to select clean plant m ...
Performance Characteristics of Three Assays for the Therapeutic
... analyzers: (i) The Abbott TDx/FLx Methotrexate II assay on the TDx/FLx analyzer as the reference method. This assay utilizes a fluorescence polarization immunoassay technique. (ii) Siemens Syva® Emit® Methotrexate assay on the Vitros 5,1 Fusion from Ortho Clinical Diagnostics. This assay utilizes a ...
... analyzers: (i) The Abbott TDx/FLx Methotrexate II assay on the TDx/FLx analyzer as the reference method. This assay utilizes a fluorescence polarization immunoassay technique. (ii) Siemens Syva® Emit® Methotrexate assay on the Vitros 5,1 Fusion from Ortho Clinical Diagnostics. This assay utilizes a ...
Identification of Novel Non-Metal Haloperoxidases from the Marine
... However, clones in group B, including CS19 HPO clone, showed relatively higher homology of approximately 80% with 70% identity as shown in Fig. 3. ...
... However, clones in group B, including CS19 HPO clone, showed relatively higher homology of approximately 80% with 70% identity as shown in Fig. 3. ...
Localization and structural analysis of the ribosomal RNA operons of
... identify and localize the position of this DNA fragment within the rRNA operons. Sequence comparisons indicated that the common PvuU fragment was internal to the rRNA operons, specifically it contained the 5' end of the 23S rRNA gene. Regions flanking the PvuU fragment were then isolated from the co ...
... identify and localize the position of this DNA fragment within the rRNA operons. Sequence comparisons indicated that the common PvuU fragment was internal to the rRNA operons, specifically it contained the 5' end of the 23S rRNA gene. Regions flanking the PvuU fragment were then isolated from the co ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher average methylation levels, more variation between subjects, and highe ...
... restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher average methylation levels, more variation between subjects, and highe ...
Genomic variations and distinct evolutionary rate of rare alleles in
... also interprets the comprehensive view of genetic architecture of the traits [11]. The genome-wide surveys of nucleotide polymorphisms in Arabidopsis thaliana have elucidated a significant departure from a neutral evolutionary model, due, in part, to an excess of rare variants [7, 12]. Substantially ...
... also interprets the comprehensive view of genetic architecture of the traits [11]. The genome-wide surveys of nucleotide polymorphisms in Arabidopsis thaliana have elucidated a significant departure from a neutral evolutionary model, due, in part, to an excess of rare variants [7, 12]. Substantially ...
Single Nucleotide Polymorphism (SNP) of the Endothelial Nitric
... accession number NP_000594) was identified using polymerase chain reaction (PCR) and sequencing analysis. The specific genotype was identified with PCR followed by restriction fragment length polymorphism using the restriction enzymes MboI and BanII (New England Bio Labs, Ipswich, Massachusetts) to ...
... accession number NP_000594) was identified using polymerase chain reaction (PCR) and sequencing analysis. The specific genotype was identified with PCR followed by restriction fragment length polymorphism using the restriction enzymes MboI and BanII (New England Bio Labs, Ipswich, Massachusetts) to ...
Control of DNA excision efficiency in Paramecium
... Therefore, at least 8 of the 15 DNAs (DNAs 156/1–7 and 156/15) harbored a ψG pseudogene macronuclear content large enough to analyze IES 156ψG-11 excision. No ∼300 bp PCR product was detected for DNAs 156/1–7, 156/9, 156/11–12 and 156/15 (Fig. 2B); instead, a PCR product of ∼330 bp was detected. In ...
... Therefore, at least 8 of the 15 DNAs (DNAs 156/1–7 and 156/15) harbored a ψG pseudogene macronuclear content large enough to analyze IES 156ψG-11 excision. No ∼300 bp PCR product was detected for DNAs 156/1–7, 156/9, 156/11–12 and 156/15 (Fig. 2B); instead, a PCR product of ∼330 bp was detected. In ...
Molecular Biology and Applied Genetics
... process that are studying in vivo, but it doesn’t necessarily tell how direct that role is. Biochemistry, by contrast, tells what a factor can do in vitro, but it doesn’t necessarily mean that it does it in vivo. The genetic and biochemical approaches tell you different things: Genetics ...
... process that are studying in vivo, but it doesn’t necessarily tell how direct that role is. Biochemistry, by contrast, tells what a factor can do in vitro, but it doesn’t necessarily mean that it does it in vivo. The genetic and biochemical approaches tell you different things: Genetics ...
Biology 32: Evolutionary Biology Computer simulations of
... Allele A1 will be lost. This is because mutation of the deleterious allele A1 into the beneficial allele A2 will only hasten the loss of allele A1 in the population. Both selection and mutation work to eliminate allele A1. 13. Reset the parameters to the default settings, but change the number of ge ...
... Allele A1 will be lost. This is because mutation of the deleterious allele A1 into the beneficial allele A2 will only hasten the loss of allele A1 in the population. Both selection and mutation work to eliminate allele A1. 13. Reset the parameters to the default settings, but change the number of ge ...
Identification of a Cis-Acting Element of ART1, a
... was able to be bound with ART1 protein and super shifted in the presence of anti-ART1 antibody (Fig. 2B). To further narrow the target region of STAR1 promoter, we divided STAR1-3 region into three parts (Fig. 1A, probes 1–3). Gel-shift assay showed that only probe 2 (2358 to 2319) was able to bind ...
... was able to be bound with ART1 protein and super shifted in the presence of anti-ART1 antibody (Fig. 2B). To further narrow the target region of STAR1 promoter, we divided STAR1-3 region into three parts (Fig. 1A, probes 1–3). Gel-shift assay showed that only probe 2 (2358 to 2319) was able to bind ...
Microsoft Word (Chapter 3) - DORAS
... Analysis of the results indicated that all siderophores tested were utilised by S. meliloti. Some background growth was evident on the plates culturing S. meliloti 2011 which was due to the production and utilisation of the endogenous siderophore rhizobactin 1021. The indicator strain S. meliloti 20 ...
... Analysis of the results indicated that all siderophores tested were utilised by S. meliloti. Some background growth was evident on the plates culturing S. meliloti 2011 which was due to the production and utilisation of the endogenous siderophore rhizobactin 1021. The indicator strain S. meliloti 20 ...
SNP genotyping
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation. An SNP is a single base pair mutation at a specific locus, usually consisting of two alleles (where the rare allele frequency is >1%). SNPs are found to be involved in the etiology of many human diseases and are becoming of particular interest in pharmacogenetics. Because SNPs are conserved during evolution, they have been proposed as markers for use in quantitative trait loci (QTL) analysis and in association studies in place of microsatellites. The use of SNPs is being extended in the HapMap project, which aims to provide the minimal set of SNPs needed to genotype the human genome. SNPs can also provide a genetic fingerprint for use in identity testing. The increase in interest in SNPs has been reflected by the furious development of a diverse range of SNP genotyping methods.