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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIOTECHNOLOGY
THIRD SEMESTER – NOV 2006
BT 3812 - PLANT BIOTECHNOLOGY
Date & Time : 25-10-2006/9.00-12.00
AE 18
Dept. No.
Max. : 100 Marks
ANSWER ALL THE QUESTIONS
1. Choose the correct answer:
1. .
2.
Genome of an organism contains
(a) haploid set of chromosome(n)
(c) triploid set of chromosome(3n)
(5 x 1= 5)
(b) diploid set of chromosome(2n)
(d) tetraploid set of chromosome (4n)
Cytoplasmic male sterility is due to
(a) chromosomal inheritance (b) extrachromosomal inheritance
(c) mitochondrial inheritance (d) cytoplasmic factors
3.
T DNA means
(a) Transfer DNA (b) Transformed DNA (c) Transposable DNA (d) Transduced
DNA
4.
USDA stands for
(a) United States Division of Agriculture (b) United States Department of
Agriculture
(c) United States Department of Administration (d) United States Division of
Administration
5.
Co-ordinated gene expression is seen in
(a) Histidine gene regulation (b) lac operon (c) Tryptophan operon (d) none of the
above
II. State True or False, if false give reasons
6.
7.
8.
9.
10.
Chlamydomonas contains chloroplast DNA
Expression vector is used for protein expression.
Agrobacterium is mainly used for gene transformation in dicots.
-gal is used as a selectable marker
Fruit ripening hormone is ethylene.
III. Complete the following:
11.
12.
13.
14.
15.
(5 x 1= 5)
Inducer present in lac operon is ________________________.
First recombinant vector was consrtucted by _______________.
First biotechnology company in India is ___________________.
Jumping gene was discovered by _________________________.
The lac operon was discovered by _____________________.
(5 x 1=5)
IV. Answer the following, not more than 50 words each
16.
17.
18.
19.
20.
(5 x 1=5)
Give an example of a natural genetic engineer and explain very briefly
Describe selectable marker
What is an aporepressor?
What is constitutive gene expression
Define signal transduction.
PART B
V. Answer any five of the following, each within 350 words only:
(5 x 8= 40)
21. Explain co-ordinated gene expression.
22. Explain transgene analysis.
23. What is a genome project? When was it started, where, by whom and give an example?
24. Explain inducible gene expression
25. Describe the methodology for somatic hybridization.
26. Explain the process involved in synthetic seed production.
27. Give an account on haploid plant production through tissue culture.
28. Describe the organization of nif genes.
PART C
VI. Answer the following in detail, not morebthan 1500 words each
29. (a) Explain in detail the transcriptional control of gene action in bacteria
OR
(b) Explain in detail extrachromosomal inheritance
30. (a) Explain the regulation of gene action at the level of transcription
OR
(b) Describe the roles of mineral ions in tissue culture media.
____________________
(2 x 20= 40)