* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download DNA technology notes
Metagenomics wikipedia , lookup
Epigenetics wikipedia , lookup
Zinc finger nuclease wikipedia , lookup
Mitochondrial DNA wikipedia , lookup
Human genome wikipedia , lookup
DNA paternity testing wikipedia , lookup
Comparative genomic hybridization wikipedia , lookup
Genome (book) wikipedia , lookup
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia , lookup
SNP genotyping wikipedia , lookup
DNA polymerase wikipedia , lookup
Nutriepigenomics wikipedia , lookup
DNA profiling wikipedia , lookup
Primary transcript wikipedia , lookup
Cancer epigenetics wikipedia , lookup
Bisulfite sequencing wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Genomic library wikipedia , lookup
DNA damage theory of aging wikipedia , lookup
Point mutation wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
DNA vaccination wikipedia , lookup
Microsatellite wikipedia , lookup
Genome editing wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
Genealogical DNA test wikipedia , lookup
Epigenomics wikipedia , lookup
Designer baby wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
Genetic engineering wikipedia , lookup
Cell-free fetal DNA wikipedia , lookup
Nucleic acid double helix wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Non-coding DNA wikipedia , lookup
DNA supercoil wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Molecular cloning wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Helitron (biology) wikipedia , lookup
DNA Technology Notes Journal 3 • Compare/contrast replication, transcription and translation. Define Genetic Engineering • Process where DNA is split into fragments and new DNA pieces are inserted • Restriction enzymes cut DNA at certain places • Carriers are then used to insert DNA splices • Now referred to as recombinant DNA or transgenic Applications of recombinant DNA • Bacteria: used to reduce frost damage to crops, break down pollutants, produce hormones and antibiotics • Plants: resistant to herbicides, increase protein content in grains • Animals: cloning of endangered species, replacement of genes which cause disorders Describe DNA fingerprinting • Samples of DNA is exposed to restriction enzyme which cuts it into pieces • DNA is loaded into a gel and run through an electrophoresis machine • Since DNA is cut at certain sequences, each piece is a different length and weight • Pieces that are heavier stay at the top of the gel, lighter will “run” to the bottom • Every person is genetically different, so DNA fingerprints are different DNA fingerprinting applications • Can be used to identify or eliminate suspects when genetic material has been collected at a crime scene • Can be used to determine paternity of a child, or establish other family relationships • Can be used to identify a deceased person Describe karyotyping • Picture of chromosomes are taken from a cell sample, cut out and matched up in pairs • Humans have 23 pairs of chromosomes • Karyotypes can be used to determine if genetic disorder is present • If too many are present can indicate Down’s syndrome • If some are missing can indicate Turner’s syndrome Gene therapy • Insertion of normal gene into human cell to correct genetic disorders • Being used now to treat cystic fibrosis Quiz 3 1. In genetic ? DNA is split and new fragments of DNA are inserted. a. deletion b. engineering c. mutation 2. Gene ? Involves the insertion of normal genes in the place of genes which control genetic disorders. a. cloning b. mutation c.therapy 3. DNA fingerprinting works because everyone’s DNA is different except for ? a. fraternal twins b. identical twins 4. The structure of DNA was discovered by ? a. Watson & Crick b. Wilson & Crockett c. Schleiden & Schwann 5. DNA gels can be used for ? a. determination of parents b. elimination of suspects c. identification of body d. all of these **