The female-killing chromosome of the silkworm, Bombyx mori, was
... pSa+pW+od chromosome, Tazima isolated the ‘Z101 strain’; this strain has a paternally transmitted mutant W chromosome, Df(pSa+pW+od)Fem. Based on genetic analysis, Tazima (1952) thought that the Df(pSa+pW+od)Fem chromosome was generated by the deletion of the Fem-containing region of the pSa+pW+od c ...
... pSa+pW+od chromosome, Tazima isolated the ‘Z101 strain’; this strain has a paternally transmitted mutant W chromosome, Df(pSa+pW+od)Fem. Based on genetic analysis, Tazima (1952) thought that the Df(pSa+pW+od)Fem chromosome was generated by the deletion of the Fem-containing region of the pSa+pW+od c ...
TRANSLOCATIONS INVOLVING T H E THIRD AND THE FOURTH
... the third chromosome, one fragment being attached to the fourthchromosome. These gametes carry, therefore, the gene D and the normal allelomorph of ev. The other kind of gametes contains the normal (that is, the unbroken) third chromosome andthe free fourth chromosome. These gametes carry ey but do ...
... the third chromosome, one fragment being attached to the fourthchromosome. These gametes carry, therefore, the gene D and the normal allelomorph of ev. The other kind of gametes contains the normal (that is, the unbroken) third chromosome andthe free fourth chromosome. These gametes carry ey but do ...
Chromosome Variation
... over, a duplication-generating process ( ◗ FIGURE 9.8; see also Figure 17.17). How does a chromosome duplication alter the phenotype? After all, gene sequences are not altered by duplications, and no genetic information is missing; the only change is the presence of additional copies of normal seque ...
... over, a duplication-generating process ( ◗ FIGURE 9.8; see also Figure 17.17). How does a chromosome duplication alter the phenotype? After all, gene sequences are not altered by duplications, and no genetic information is missing; the only change is the presence of additional copies of normal seque ...
Product description P018-G1 SHOX-v03 - MRC
... To be used in combination with a SALSA® MLPA® reagent kit, available for various number of reactions. MLPA reagent kits are either provided with FAM or Cy5.0 dye-labelled PCR primer, suitable for Applied Biosystems and Beckman capillary sequencers, respectively (see www.mlpa.com). Certificate of Ana ...
... To be used in combination with a SALSA® MLPA® reagent kit, available for various number of reactions. MLPA reagent kits are either provided with FAM or Cy5.0 dye-labelled PCR primer, suitable for Applied Biosystems and Beckman capillary sequencers, respectively (see www.mlpa.com). Certificate of Ana ...
TaqMan-Based Real-Time PCR for Genotyping Common
... and HP2 for typing (14, 15 ). More recently, a singlechain antibody-based ELISA test was also established (16 ). In addition, several HP-genotyping methods based on conventional PCR have been developed; however, these PCR-based methods need to amplify a relatively large DNA fragment to detect the 1. ...
... and HP2 for typing (14, 15 ). More recently, a singlechain antibody-based ELISA test was also established (16 ). In addition, several HP-genotyping methods based on conventional PCR have been developed; however, these PCR-based methods need to amplify a relatively large DNA fragment to detect the 1. ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... DNA damage. More importantly, these dyes and how they bind to DNA gave important clues about the actual structure-function relationship of the DNA molecule. As an aside, it is interesting to note that the very dyes that were used in Peacocke’s pioneering studies with DNA structure are still used in ...
... DNA damage. More importantly, these dyes and how they bind to DNA gave important clues about the actual structure-function relationship of the DNA molecule. As an aside, it is interesting to note that the very dyes that were used in Peacocke’s pioneering studies with DNA structure are still used in ...
Analyzing Loss of Heterozygosity in in Partek Genomics Suite™ v6.6
... may be used in conjunction with duo or trio workflows to detect identity by descent (IBD). To learn more on duo and trio workflows, please consult the respective documentation. An advantage of the LOH analysis is that it provides a solution to a problem associated with the copy number approach: the ...
... may be used in conjunction with duo or trio workflows to detect identity by descent (IBD). To learn more on duo and trio workflows, please consult the respective documentation. An advantage of the LOH analysis is that it provides a solution to a problem associated with the copy number approach: the ...
Real-time PCR Handbook
... The Ct is used to calculate the initial DNA copy number, because the Ct value is inversely related to the starting amount of target. For example, in comparing real-time PCR results from samples containing different amounts of target, a sample with twice the starting amount will yield a Ct one cycle ...
... The Ct is used to calculate the initial DNA copy number, because the Ct value is inversely related to the starting amount of target. For example, in comparing real-time PCR results from samples containing different amounts of target, a sample with twice the starting amount will yield a Ct one cycle ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... Present address: Code 6030, Laboratory for the Structure of Matter, Naval Research Laboratory, Washington DC 20375-5000, USA ...
... Present address: Code 6030, Laboratory for the Structure of Matter, Naval Research Laboratory, Washington DC 20375-5000, USA ...
Designing synthetic MLPA probes - MRC
... Hybridising parts of LPO and RPO. Bind to adjacent target DNA sequences. Probe oligonucleotide that is situated on the left when probe is shown from 5’ to 3’. Consists of forward PCR primer sequence (5’ end) and hybridising sequence (3’ end). 3’ nucleotide of LPO and 5’ nucleotide of RPO, where liga ...
... Hybridising parts of LPO and RPO. Bind to adjacent target DNA sequences. Probe oligonucleotide that is situated on the left when probe is shown from 5’ to 3’. Consists of forward PCR primer sequence (5’ end) and hybridising sequence (3’ end). 3’ nucleotide of LPO and 5’ nucleotide of RPO, where liga ...
Good quality blastocyst from non-/mono
... Furthermore, there is limited research on the morphology of healthy embryos [Palini et al. 2015]. We did not investigate the implantation rate and clinical parameter for these blastocysts. Thus, further studies are needed before ‘abnormal embryos’ can be used for transfer in the clinic. In this stud ...
... Furthermore, there is limited research on the morphology of healthy embryos [Palini et al. 2015]. We did not investigate the implantation rate and clinical parameter for these blastocysts. Thus, further studies are needed before ‘abnormal embryos’ can be used for transfer in the clinic. In this stud ...
Control of DNA excision efficiency in Paramecium
... within F1 progeny relative to F2 progeny demonstrates that Paramecium IES excision efficiency is sensitive either to a conjugation-specific trans-acting factor provided by the zygotic genome, or to homologous chromosome cross-talk. INTRODUCTION Programmed DNA rearrangements occur in a wide range of ...
... within F1 progeny relative to F2 progeny demonstrates that Paramecium IES excision efficiency is sensitive either to a conjugation-specific trans-acting factor provided by the zygotic genome, or to homologous chromosome cross-talk. INTRODUCTION Programmed DNA rearrangements occur in a wide range of ...
Figures 1
... and feet grow slowly and are generally below the fifth percentile by age 10 years. Data from at least 15 studies involving more than 300 affected children document reduced GH secretion in PWS.33 Best practice in early intervention for PWS includes recommendations for GH therapy.4,34 GH therapy decre ...
... and feet grow slowly and are generally below the fifth percentile by age 10 years. Data from at least 15 studies involving more than 300 affected children document reduced GH secretion in PWS.33 Best practice in early intervention for PWS includes recommendations for GH therapy.4,34 GH therapy decre ...
Properties of Mitotic and Meiotic Recombination in the
... most 5-FOAR HygS GenS CanR derivatives are a consequence of crossovers rather than chromosome loss. We also examined 5-FOAR HygS GenS CanR derivatives of YZ113 to determine whether these strains were monosomic. Since both homologs of YZ113 were derived from W303-1A, we could not use the PCR-based di ...
... most 5-FOAR HygS GenS CanR derivatives are a consequence of crossovers rather than chromosome loss. We also examined 5-FOAR HygS GenS CanR derivatives of YZ113 to determine whether these strains were monosomic. Since both homologs of YZ113 were derived from W303-1A, we could not use the PCR-based di ...
SALSA MLPA probemix P018-F1 SHOX - MRC
... This P018-F1 SHOX probemix contains probes for each exon of the human SHOX gene, as well as a probe just before the SHOX promoter region. In addition, several probes are present detecting sequences in a region downstream of SHOX which has been implicated in regulation of SHOX transcription. Furtherm ...
... This P018-F1 SHOX probemix contains probes for each exon of the human SHOX gene, as well as a probe just before the SHOX promoter region. In addition, several probes are present detecting sequences in a region downstream of SHOX which has been implicated in regulation of SHOX transcription. Furtherm ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... • Gel electrophoresis is a method used to size fractionate, visualise and document DNA samples, such as those resulting from PCR • As DNA molecules are negatively charged, a current can be applied to a gel to draw the fragments toward the cathode (positive pole) • Larger fragments move more slowly t ...
... • Gel electrophoresis is a method used to size fractionate, visualise and document DNA samples, such as those resulting from PCR • As DNA molecules are negatively charged, a current can be applied to a gel to draw the fragments toward the cathode (positive pole) • Larger fragments move more slowly t ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.