Xq28 duplications
... chromosome analysis was ‘normal’. A laboratory technique called FISH (fluorescence in situ hybridisation) enables sections of the chromosome to be analysed in more detail and can help detect a duplication. This technique uses fluorescently labelled pieces of DNA that match the DNA in specific places ...
... chromosome analysis was ‘normal’. A laboratory technique called FISH (fluorescence in situ hybridisation) enables sections of the chromosome to be analysed in more detail and can help detect a duplication. This technique uses fluorescently labelled pieces of DNA that match the DNA in specific places ...
14 Chromosomes
... A small plant (Colchicum autumnale) that grows across southern Europe has the common names meadow saffron, autumn crocus and naked lady. The name ‘naked lady’ is due to the fact that after the leaves of the plant appear in spring they die off, and the flowers appear in autumn on their own (see figur ...
... A small plant (Colchicum autumnale) that grows across southern Europe has the common names meadow saffron, autumn crocus and naked lady. The name ‘naked lady’ is due to the fact that after the leaves of the plant appear in spring they die off, and the flowers appear in autumn on their own (see figur ...
2004-009_-Draft-Anne..
... Diagnosis of fire blight can be achieved using isolation and serological and molecular tests. The assays indicated below are recommended after having been evaluated in one or more of the following ring tests: in 2003 in a Diagnostic Protocols for Organisms Harmful to Plants (DIAGPRO) project involvi ...
... Diagnosis of fire blight can be achieved using isolation and serological and molecular tests. The assays indicated below are recommended after having been evaluated in one or more of the following ring tests: in 2003 in a Diagnostic Protocols for Organisms Harmful to Plants (DIAGPRO) project involvi ...
Chromosomes in Saccharomyces cerevisiae
... could reflect the shorter overall size of the artificial chromosome, the smaller separation between its centromere and telomeres, or the presence on natural chromosomes of previously unidentified specialized sequences that are required for accurate chromosome segregation. We examined the role of tel ...
... could reflect the shorter overall size of the artificial chromosome, the smaller separation between its centromere and telomeres, or the presence on natural chromosomes of previously unidentified specialized sequences that are required for accurate chromosome segregation. We examined the role of tel ...
Loss of heterozygosity at D8S262: an early genetic event of
... that surveillance of the at-risk cirrhotic population could aid earlier detection of the disease and decrease the cancer-related mortality rate, but we are limited by the lack of sensitive biomarkers and reliable histopathological features of precancerous lesions. ...
... that surveillance of the at-risk cirrhotic population could aid earlier detection of the disease and decrease the cancer-related mortality rate, but we are limited by the lack of sensitive biomarkers and reliable histopathological features of precancerous lesions. ...
International Plant Protection Convention Draft annex to ISPM 27
... Alternatively, synthetic positive controls can be made with a known sequence which again can be compared to PCR amplicons of the correct size. ...
... Alternatively, synthetic positive controls can be made with a known sequence which again can be compared to PCR amplicons of the correct size. ...
Further manipulation by centric misdivision of the 1RS.1BL
... 1RSv .1BLp were recovered. The new centric translocations 1RSv .1BLp differ from the original translocation 1RS.1BL of ‘Kavkaz’ by the 1BL arm. The reconstructed chromosome 1RSv .1RLe was moved by monosomic shift from its original position in substitution for chromosome 1B to substitutions for chrom ...
... 1RSv .1BLp were recovered. The new centric translocations 1RSv .1BLp differ from the original translocation 1RS.1BL of ‘Kavkaz’ by the 1BL arm. The reconstructed chromosome 1RSv .1RLe was moved by monosomic shift from its original position in substitution for chromosome 1B to substitutions for chrom ...
Apolipoprotein E Allele Distribution in Trisomy
... apoE alleles in the development of trisomies needs further study. ...
... apoE alleles in the development of trisomies needs further study. ...
Gene Detection Systems Catalog
... the particular changes to be made and the effect, if any, of such changes on the price and time of delivery. Buyer may not cancel this order unless such cancellation is expressly agreed to in writing by Seller. In such event, Seller will advise Buyer of the total charge for such cancellation, and Bu ...
... the particular changes to be made and the effect, if any, of such changes on the price and time of delivery. Buyer may not cancel this order unless such cancellation is expressly agreed to in writing by Seller. In such event, Seller will advise Buyer of the total charge for such cancellation, and Bu ...
1 - IPPC
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
Polymerase Chain Reaction In Ophthalmology
... the basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khurana in 19712. The PCR is superior in terms of sensitivity, specificity and rapidity of other diagnostic tests in the armamentarium. The presence of DNA or RNA of the pathogen can directly be ...
... the basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khurana in 19712. The PCR is superior in terms of sensitivity, specificity and rapidity of other diagnostic tests in the armamentarium. The presence of DNA or RNA of the pathogen can directly be ...
Characterisation of interstitial duplications and triplications of
... showed that, in all cases, the duplications and triplications involved the PWACR and were not pseudogene expansions. Retrospective cytogenetic analysis in families 7 and 13 did not identify these duplications clearly. The size of the pericentromeric area of 15q varies greatly within the normal popul ...
... showed that, in all cases, the duplications and triplications involved the PWACR and were not pseudogene expansions. Retrospective cytogenetic analysis in families 7 and 13 did not identify these duplications clearly. The size of the pericentromeric area of 15q varies greatly within the normal popul ...
DNA sentences How are proteins coded for by DNA?
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a doublestranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribon ...
... Deoxyribonucleic acid (DNA) is the molecule of life. DNA is one of the most recognizable nucleic acids, a doublestranded helix. The process by which DNA codes for proteins involves enzymes and additional single-stranded nucleic acids, specifically messenger ribonucleic acid (mRNA) and transfer ribon ...
DNA sentences - seed2stem.org
... Choose one person to be the transcriber. Find the DNA strands assigned located at the table in the center of the room. 2. On the data sheet provided, transcribe the mRNA codons from the DNA strand (without moving the DNA). 3. At the group table, choose a different person to translate the mRNA codo ...
... Choose one person to be the transcriber. Find the DNA strands assigned located at the table in the center of the room. 2. On the data sheet provided, transcribe the mRNA codons from the DNA strand (without moving the DNA). 3. At the group table, choose a different person to translate the mRNA codo ...
Chromosomes Carrying Meiotic Avoidance Loci
... BACs from contig A and another pool of four BACs from contig B (Fig. 1). The Hieracium genome has not been sequenced, and the absence of a reference genome coupled with the repetitive sequence nature of the BACs hampered the assembly of DNA sequences. A total of 379 nonredundant contigs were assembl ...
... BACs from contig A and another pool of four BACs from contig B (Fig. 1). The Hieracium genome has not been sequenced, and the absence of a reference genome coupled with the repetitive sequence nature of the BACs hampered the assembly of DNA sequences. A total of 379 nonredundant contigs were assembl ...
Isolation, Characterization, and Annotation: The Search for Novel
... genomic DNA was then eluted from each column by adding pre-warmed 80°C TE to the resin in the column. After 30 seconds, the columns were centrifuged for 1 minute in microcentrifuge tubes. The DNA samples were then transferred into clean tubes and stored at 4°C.To check the quantity of DNA, the resea ...
... genomic DNA was then eluted from each column by adding pre-warmed 80°C TE to the resin in the column. After 30 seconds, the columns were centrifuged for 1 minute in microcentrifuge tubes. The DNA samples were then transferred into clean tubes and stored at 4°C.To check the quantity of DNA, the resea ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.