The Parasexual Cycle in Candida albicans Provides an
... been observed. Instead, tetraploid products of mating can be induced to undergo efficient, random chromosome loss, often producing strains that are diploid, or close to diploid, in ploidy. Using SNP and comparative genome hybridization arrays we have now analyzed the genotypes of products from the C ...
... been observed. Instead, tetraploid products of mating can be induced to undergo efficient, random chromosome loss, often producing strains that are diploid, or close to diploid, in ploidy. Using SNP and comparative genome hybridization arrays we have now analyzed the genotypes of products from the C ...
Forche et al. 2008 PLoS Biology
... been observed. Instead, tetraploid products of mating can be induced to undergo efficient, random chromosome loss, often producing strains that are diploid, or close to diploid, in ploidy. Using SNP and comparative genome hybridization arrays we have now analyzed the genotypes of products from the C ...
... been observed. Instead, tetraploid products of mating can be induced to undergo efficient, random chromosome loss, often producing strains that are diploid, or close to diploid, in ploidy. Using SNP and comparative genome hybridization arrays we have now analyzed the genotypes of products from the C ...
Tag-ItTM Mutation Detection Kit for CFTR 70+6
... Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
... Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
Post-PCR sterilization: a method to control carryover contamination
... the introduction of automated DNA synthesizers. Short, synthetic oligonucleotides provided new tools for the advancement of cloning techniques, the creation of allele-specific hybridization protocols and, most importantly, template-dependent polymerase extension reactions were developed into nucleic ...
... the introduction of automated DNA synthesizers. Short, synthetic oligonucleotides provided new tools for the advancement of cloning techniques, the creation of allele-specific hybridization protocols and, most importantly, template-dependent polymerase extension reactions were developed into nucleic ...
BIO 402 - National Open University of Nigeria
... is was the first drawing ever made of cells. Microscopes at that time magnified 100 to 200 times with a distortion of shape and color that increased with magnification. Nevertheless, these microscopes revealed many new things. Still, it was necessary to wait for better lenses to see anything more. S ...
... is was the first drawing ever made of cells. Microscopes at that time magnified 100 to 200 times with a distortion of shape and color that increased with magnification. Nevertheless, these microscopes revealed many new things. Still, it was necessary to wait for better lenses to see anything more. S ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... detection. The two pairs of GMO primers in the kit will amplify two DNA sequences, a 203 base pair (bp) fragment of the cauliflower mosaic virus (CaMV) 35S promoter and a 225 bp fragment of the nopaline synthase (NOS) terminator. The kit is designed to use multiplex PCR with the GMO reactions so tha ...
... detection. The two pairs of GMO primers in the kit will amplify two DNA sequences, a 203 base pair (bp) fragment of the cauliflower mosaic virus (CaMV) 35S promoter and a 225 bp fragment of the nopaline synthase (NOS) terminator. The kit is designed to use multiplex PCR with the GMO reactions so tha ...
background of the invention
... type biosensor executing a DNA sequence amplification in accordance with an embodiment of the present invention, the single-stranded DNA nanotemplate 51 is situated on a surface 54 of a gate 53 of a field-effect transistor 52 of a field-effect transistor chip manufactured according to the manufactur ...
... type biosensor executing a DNA sequence amplification in accordance with an embodiment of the present invention, the single-stranded DNA nanotemplate 51 is situated on a surface 54 of a gate 53 of a field-effect transistor 52 of a field-effect transistor chip manufactured according to the manufactur ...
Detection of GM Papaya Event 55-1 in Fresh
... Duplex PCR analysis was developed to detect a genetically modified (GM) papaya event 55-1 in both fresh and processed papaya fruit. GM papaya event 55-1 is a genetically modified organism (GMO) not currently approved for food in Korea. Using a primer set specific to papain, an endogenous papaya gene ...
... Duplex PCR analysis was developed to detect a genetically modified (GM) papaya event 55-1 in both fresh and processed papaya fruit. GM papaya event 55-1 is a genetically modified organism (GMO) not currently approved for food in Korea. Using a primer set specific to papain, an endogenous papaya gene ...
8 VARIATION IN CHROMOSOME STRUCTURE AND NUMBER
... variety of shapes and sizes, let’s consider how the structures of normal chromosomes can be modified. In some cases, the total amount of genetic material within a single chromosome can be increased or decreased significantly. Alternatively, the genetic material in one or more chromosomes may be rear ...
... variety of shapes and sizes, let’s consider how the structures of normal chromosomes can be modified. In some cases, the total amount of genetic material within a single chromosome can be increased or decreased significantly. Alternatively, the genetic material in one or more chromosomes may be rear ...
Locked Nucleic Acid (LNA™)
... and other small RNA molecules. Exiqon offers a range of LNA™-based products for microRNA detection, quantitation and antisense inhibition (see box to the left). The unique ability of LNA™ oligonucleotides to discriminate between highly similar sequences has been exploited in a number of applications ...
... and other small RNA molecules. Exiqon offers a range of LNA™-based products for microRNA detection, quantitation and antisense inhibition (see box to the left). The unique ability of LNA™ oligonucleotides to discriminate between highly similar sequences has been exploited in a number of applications ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
... containing MLH1 or MSH2 exon deletions or amplifications: http://www.nibsc.org/documents/ifu/11-218xxx.pdf. As an alternative, an artificial positive duplication DNA sample for MLH1 and MSH2 (product name SD027) can be ordered from MRC-Holland. This FOR RESEARCH USE ONLY SD027 DNA will show a duplic ...
... containing MLH1 or MSH2 exon deletions or amplifications: http://www.nibsc.org/documents/ifu/11-218xxx.pdf. As an alternative, an artificial positive duplication DNA sample for MLH1 and MSH2 (product name SD027) can be ordered from MRC-Holland. This FOR RESEARCH USE ONLY SD027 DNA will show a duplic ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
The Deletion Stocks of Common Wheat
... deletion chromosome. deletion L arm tion chromosome had some chromosomal progeny for homozygous plants with deaberrations. For the production of deleletion chromosomes and the least degree The arm ratios were used to compensate of aberrations in the other chromosomes, for the difference in contracti ...
... deletion chromosome. deletion L arm tion chromosome had some chromosomal progeny for homozygous plants with deaberrations. For the production of deleletion chromosomes and the least degree The arm ratios were used to compensate of aberrations in the other chromosomes, for the difference in contracti ...
ARTICLES - Weizmann Institute of Science
... Indeed, several types of regions had markedly high or low predicted occupancy. The highest predicted occupancy was over centromeres, indicating that centromere function requires enhanced stability of histone–DNA interactions that are encoded in the genomic sequence. One might think that genomes woul ...
... Indeed, several types of regions had markedly high or low predicted occupancy. The highest predicted occupancy was over centromeres, indicating that centromere function requires enhanced stability of histone–DNA interactions that are encoded in the genomic sequence. One might think that genomes woul ...
Resolving Individuals Contributing Trace Amounts of DNA to Highly
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
22q12 and 22q13 duplications
... shows gains and losses of tiny amounts of DNA throughout the chromosomes. Microarrays can also show whether particular genes are duplicated or not. The break points in chromosome 22 vary from person to person, so each person will have a different number of genes duplicated. The smallest duplications ...
... shows gains and losses of tiny amounts of DNA throughout the chromosomes. Microarrays can also show whether particular genes are duplicated or not. The break points in chromosome 22 vary from person to person, so each person will have a different number of genes duplicated. The smallest duplications ...
uncorrected page proofs
... A small plant (Colchicum autumnale) that grows across southern Europe has the common names meadow saffron, autumn crocus and naked lady. The name ‘naked lady’ is due to the fact that after the leaves of the plant appear in spring they die off, and the flowers appear in autumn on their own (see figure ...
... A small plant (Colchicum autumnale) that grows across southern Europe has the common names meadow saffron, autumn crocus and naked lady. The name ‘naked lady’ is due to the fact that after the leaves of the plant appear in spring they die off, and the flowers appear in autumn on their own (see figure ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.