by Attila Mokanszki Supervisor: Prof. Dr. Eva Olah
... cancers play a central role in determining the high mortality rate. The Hungarian population started to decrease at the beginning of 80s years, after the millenary it became 40 000 fewer every year. Today the number of Hungarian population is fewer than 10 million. In the background of this decline ...
... cancers play a central role in determining the high mortality rate. The Hungarian population started to decrease at the beginning of 80s years, after the millenary it became 40 000 fewer every year. Today the number of Hungarian population is fewer than 10 million. In the background of this decline ...
Press release
... protocol and secondly also on the starting weight or volume. It also depends to a large extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which i ...
... protocol and secondly also on the starting weight or volume. It also depends to a large extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which i ...
Bacteriophage A cloning system for the construction of
... We have developed a bacteriophage A cloning vector, XORF8, that can be used for the construction of cDNA libraries. The wild-type A genome contains five BamHI, five EcoRI, and seven Hindu restriction sites that have all been removed from the genome of AORF8. Sites for these endonucleases are present ...
... We have developed a bacteriophage A cloning vector, XORF8, that can be used for the construction of cDNA libraries. The wild-type A genome contains five BamHI, five EcoRI, and seven Hindu restriction sites that have all been removed from the genome of AORF8. Sites for these endonucleases are present ...
CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI The
... Nature. Author manuscript; available in PMC 2016 February 04. Published in final edited form as: ...
... Nature. Author manuscript; available in PMC 2016 February 04. Published in final edited form as: ...
Structural changes following the reversal of a Y chromosome to an
... translocations can also reverse sex chromosomes back into autosomes, which is especially intriguing given that autosomes and sex chromosomes differ in gene regulation and chromatin environment. While researchers are beginning to understand X chromosomes reversals to autosomes at a genomic level, it ...
... translocations can also reverse sex chromosomes back into autosomes, which is especially intriguing given that autosomes and sex chromosomes differ in gene regulation and chromatin environment. While researchers are beginning to understand X chromosomes reversals to autosomes at a genomic level, it ...
Recall Questions
... Polyploidy: Gain of entire sets of chromosomes so the chromosome number changes from 2n to 3n (triploid), 4n (tetraploid), and so on. *2. Why do extra copies of genes sometimes cause drastic phenotypic effects? The expression of some genes is balanced with the expression of other genes; the ratios o ...
... Polyploidy: Gain of entire sets of chromosomes so the chromosome number changes from 2n to 3n (triploid), 4n (tetraploid), and so on. *2. Why do extra copies of genes sometimes cause drastic phenotypic effects? The expression of some genes is balanced with the expression of other genes; the ratios o ...
2004-011: Draft Annex to ISPM 27:2006 – Xanthomonas citri subsp
... Aliquots of 25 µl of each bacterial preparation or plant sample to be tested are pipetted onto a plastic-coated multi-window microscope slide, allowed to air dry and then gently heat-fixed over a flame. Separate slides are set up for each test bacterium, and also for positive and negative controls a ...
... Aliquots of 25 µl of each bacterial preparation or plant sample to be tested are pipetted onto a plastic-coated multi-window microscope slide, allowed to air dry and then gently heat-fixed over a flame. Separate slides are set up for each test bacterium, and also for positive and negative controls a ...
Electrophoretic karyotypes of clinically isolated yeasts
... range in a single gel, and also under conditions (Fig. 1b) in which chromosomes smaller than 2.0 Mb were better separated. The karyotypes of many isolates were similar to that of FC18, whose chromosome sizes have been calibrated as 2.86, 2-66, 2.20, 1.90, 1.70, 1.36, 1-18 and 1.08 Mb. These chromoso ...
... range in a single gel, and also under conditions (Fig. 1b) in which chromosomes smaller than 2.0 Mb were better separated. The karyotypes of many isolates were similar to that of FC18, whose chromosome sizes have been calibrated as 2.86, 2-66, 2.20, 1.90, 1.70, 1.36, 1-18 and 1.08 Mb. These chromoso ...
Female Male Human chromosomal abnormalities may be numerical
... Consider the following results with two chromosome 21 SSRs in a child with Down syndrome and ...
... Consider the following results with two chromosome 21 SSRs in a child with Down syndrome and ...
DNA Evidence: Probability, Population Genetics, and the Courts
... n.17 (1991). A case that is representative of this epoch is Cobey v. State, 559 A.2d 391 (Md. Ct. Spec. App. 1988). A man forced a woman jogging in a park into the woods, where, as the court of appeals put it, he "ravished" her and drove away in her car. A policeman issued a traffic citation to Kenn ...
... n.17 (1991). A case that is representative of this epoch is Cobey v. State, 559 A.2d 391 (Md. Ct. Spec. App. 1988). A man forced a woman jogging in a park into the woods, where, as the court of appeals put it, he "ravished" her and drove away in her car. A policeman issued a traffic citation to Kenn ...
2. Assessing the probative value of DNA evidence
... probative value in criminal trials. The remaining two Guides will give detailed consideration to: (3) networks for structuring evidence; and (4) principles of forensic case assessment and interpretation. Each of these topics has major practical importance, and therefore merits sustained investigatio ...
... probative value in criminal trials. The remaining two Guides will give detailed consideration to: (3) networks for structuring evidence; and (4) principles of forensic case assessment and interpretation. Each of these topics has major practical importance, and therefore merits sustained investigatio ...
Assessing the Probative Value of DNA Evidence
... Criminal adjudication in the UK’s legal jurisdictions is strongly wedded to the principle of lay fact-finding by juries and magistrates employing their ordinary common sense reasoning. Notwithstanding the unquestionable merits of lay involvement in criminal trials, it cannot be assumed that jurors o ...
... Criminal adjudication in the UK’s legal jurisdictions is strongly wedded to the principle of lay fact-finding by juries and magistrates employing their ordinary common sense reasoning. Notwithstanding the unquestionable merits of lay involvement in criminal trials, it cannot be assumed that jurors o ...
MycoplasMa Quality control
... These adverse effects can lead to the misinterpretation of results and compromise the validity of data generated for research and/or development projects. Importance of routine testing Because mycoplasma can affect the phenotypic and functional characteristics of cells in vitro, the implementation o ...
... These adverse effects can lead to the misinterpretation of results and compromise the validity of data generated for research and/or development projects. Importance of routine testing Because mycoplasma can affect the phenotypic and functional characteristics of cells in vitro, the implementation o ...
The whole paper can be downloaded here if you like.
... The third and most important portion of the study was DNA fingerprinting. This consisted of two parts. The first part was the restriction enzyme digest. The restriction enzyme digest used on the plasmid had two enzymes, Hind III and Ava II. This was a good combination of enzymes because it cut all o ...
... The third and most important portion of the study was DNA fingerprinting. This consisted of two parts. The first part was the restriction enzyme digest. The restriction enzyme digest used on the plasmid had two enzymes, Hind III and Ava II. This was a good combination of enzymes because it cut all o ...
Equilibrium Statistics of Channel-confined DNA
... This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels with a width of about 100 nm. The resea ...
... This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels with a width of about 100 nm. The resea ...
one-step and stepwise magnification of a bobbed lethal
... Squashes of larval neuroblasts were prepared, stained with the fluorochrome DAPI and scanned under fluorescence for metaphase figures. The y bb' chromosome is a typical rod-shaped X chromosome (Figure la) in which the short arm is sometimes visible. The Ybby+chromosome consists of a long arm with th ...
... Squashes of larval neuroblasts were prepared, stained with the fluorochrome DAPI and scanned under fluorescence for metaphase figures. The y bb' chromosome is a typical rod-shaped X chromosome (Figure la) in which the short arm is sometimes visible. The Ybby+chromosome consists of a long arm with th ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... last four codons of the repressor gene fused to the 25th codon of the lacZ gene. The frameshift mutation (an extra ‘‘C’’ in the 320th ‘‘CCC’’ codon of lacI) lies at the end the lacI gene, creating a ⫹1 frameshift polar to lacZ. The phenotype of a strain bearing the lacI33 allele is therefore Lac⫺, b ...
... last four codons of the repressor gene fused to the 25th codon of the lacZ gene. The frameshift mutation (an extra ‘‘C’’ in the 320th ‘‘CCC’’ codon of lacI) lies at the end the lacI gene, creating a ⫹1 frameshift polar to lacZ. The phenotype of a strain bearing the lacI33 allele is therefore Lac⫺, b ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.